Labshake search
Citations for BioLegend :
601 - 650 of 752 citations for 6 amino 7H benzo e perimidin 7 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... Samples that were stained for cell surface staining only were incubated for 5 min on ice in 7-AAD (Biolegend, #420404). Samples that were stained for intracellular markers were fixed and permeabilized using the eBioscience™ FoxP3/Transcription Factor Staining Buffer Set (Thermofisher ...
-
bioRxiv - Immunology 2022Quote: CD4+ T cells (1 × 106) were stimulated for 7 days at 37°C with anti-mouse CD3 (BioLegend; 1 μg/mL) and CD28 (BioLegend ...
-
bioRxiv - Cell Biology 2019Quote: ... blocked with 1 mg/mL human IgG (Privigen, Behring CSL) and stained with mAb 7–239 α-Siglec-1-PE or matched isotype-PE control (Biolegend) at 4°C for 30 min ...
-
bioRxiv - Immunology 2022Quote: ... IL-2 and IL-7 were removed from ILC2 culture and additionally cultured for 4 hr before restimulation with IL-33 (Biolegend, #580508) plus NMU (R&D systems ...
-
bioRxiv - Developmental Biology 2023Quote: The flow cytometry staining for necrosis and apoptosis was was performed with the FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend, 640922). Once the cells were incubated in 10 µM of EdU ...
-
bioRxiv - Cancer Biology 2024Quote: Apoptotic and necrotic cell analysis was performed using Pacific Blue™ Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend, #640926) as per manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... one million cells were first resuspended in 100 μL buffer containing 5 μL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15 minutes ...
-
bioRxiv - Immunology 2019Quote: ... Supernatants were collected at 2 hours and 4 hours and analyzed using IL-6 (Biolegend). After removal of supernatant ...
-
bioRxiv - Immunology 2021Quote: ... The splenocytes were incubated for 6 hr at 37°C in vitro with BrefeldinA (Biolegend) under three conditions ...
-
bioRxiv - Microbiology 2020Quote: ... PBS supplemented with 0.1 µg/ml antifade-4 6-diamidino-2-phenylindole (DAPI) (BioLegend, USA) was added to the cells for at least 30 min before imaging ...
-
bioRxiv - Neuroscience 2022Quote: Enzyme-linked immunosorbent assays were performed using the mouse IL-6 ELISA-MAX kit (Biolegend), using manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2022Quote: NSG mice were administrated intraperitoneally with IL-6 (50 μg /mouse) neutralizing Ab (BioLegend 504512) for two days ...
-
bioRxiv - Immunology 2020Quote: ... and then stimulated with 10nM HyIL-6 or 0.1 μg/mL anti-CD3 (Biolegend #300438) and 20 ng/mL IL2 (Novartis #709421) ...
-
bioRxiv - Immunology 2021Quote: ... and supernatants were harvested for IL-6 and IL-1β ELISA (Biolegend, San Diego, CA). BMDCs were also utilized in the MLR and in vitro T cell differentiation assays.
-
bioRxiv - Immunology 2022Quote: ... and then stained with PE-conjugated anti-mouse IL-6 mAb (clone MP5-20F3, Biolegend). Stained cells were analysed using a BD LSR Fortessa cell analyser (BD Biosciences ...
-
bioRxiv - Immunology 2023Quote: ... The nuclei were stained with 3 µM DAPI (4’,6-diamidino-2-phenylindole; BioLegend, #422801) for 5 min ...
-
bioRxiv - Bioengineering 2023Quote: ... and IL-4 and IL-6 using a LEGENDplex™ mouse inflammation cytokine assay (Biolegend). Collected blood was incubated at room temperature for 1 hr and centrifuged for 7 min at 12,000 g at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... and PE anti-mouse H-2Kb/H2Db antibody (Clone 28-8-6; Biolegend; Cat #114607) on ice for 20 minutes in the dark ...
-
bioRxiv - Immunology 2023Quote: ... macrophages were challenged with CFSE (5-[and 6]-Carboxyfluorescein diacetate succinimidyl ester Kit, BioLegend,423801)-labeled apoptotic cells at a 4:1 (AC/Macrophage ...
-
bioRxiv - Immunology 2024Quote: WT C57Bl/6 mice were injected intraperitoneally with 250 ng recombinant mouse IL-33 (Biolegend) in 100 µl PBS for three consecutive days ...
-
bioRxiv - Immunology 2022Quote: Ten 10-week-old male wild type C57BL/6J mice were intravenously injected with an APC/Cyanine 7-conjugated anti-mouse CD45.2 antibody (Clone 104, BioLegend 109824, RRID: AB_830789) five minutes prior to euthanasia by CO2 inhalation ...
-
bioRxiv - Cancer Biology 2021Quote: ... and early/late apoptosis was measured by using the PE Annexin V apoptosis detection kit with 7-AAD (Biolegend, San Diego, USA) according to the manufacturer ...
-
bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
-
bioRxiv - Immunology 2023Quote: ... PerCP/Cy5.5-conjugated anti-mouse Ly6G mAb (Cat. #.: 127615) and 7-AAD Viability Staining Solution (Cat. #.: 420404) were purchased from BioLegend (CA, USA).
-
bioRxiv - Cell Biology 2021Quote: ... 0.1% Sodium Azide and with one of the following primary antibody dilutions: Mouse Anti-HA (1:2000; HA.11, Biolegend), Mouse Anti-V5 (1:2000 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Then the tissues were washed multiple times in PBS at room temperature for one day and put into a staining solution containing Alexa Fluor 488 anti-mouse CD41 antibody (clone MWReg30, BioLegend) at a 1:100 dilution and Alexa Fluor 555 donkey anti-goat antibody (ThermoFisher ...
-
bioRxiv - Cell Biology 2022Quote: ... Stained cells and “fluorescence minus one” controls were then resuspended in SB + 1:1000 DNase + 1:1000 Draq7 (Biolegend, #424001) as a live/dead stain ...
-
bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in TBS-T/5% BSA for one hour at RT and incubated overnight at 4 °C with anti-P62 (SQSTM1) (1:500, #814802, BioLegend), anti-CD107b (LAMP-2 ...
-
bioRxiv - Cell Biology 2022Quote: ... bone marrow cells were incubated with cell surface markers for one hour at 4 °C and then washed with PBS before being resuspended with Annexin V binding buffer (Biolegend). Cells were then incubated with FITC Annexin V (Biolegend ...
-
bioRxiv - Cell Biology 2022Quote: One million fixed BM and spleen cells were immunolabelled for one hour at room temperature with Ter119-PE (1:200, Biolegend) and 1ug/ml Hoechst 33342 (Thermofisher ...
-
bioRxiv - Immunology 2021Quote: ... Cells were stained with fluorescently-labeled antibodies directed against cell-specific surface marker for 30 min one ice in the dark (anti-mouse CD45-APC/Cy7, #103116, BioLegend; anti-mouse CD45-FITC ...
-
bioRxiv - Immunology 2022Quote: ... Each of the three biological replicates that were treated with PBS vehicle control or TGM was labeled with one of three unique TotalSeq Hashtags Antibody markers (Biolegend). The samples were individually sort-purified by gating CD45+ cells and then each treatment group was pooled with an even number of cells from each sample ...
-
bioRxiv - Immunology 2023Quote: ... Cells were incubated at 37°C and 5% CO2 for one hour to settle and then stimulated with 20 ng/mL recombinant IFNg (BioLegend), 100 ng/mL LPS-EB Ultrapure (Invivogen ...
-
bioRxiv - Immunology 2023Quote: ... Each sample was labelled with one specific hashtag (1:400 dilution) and CITE-seq antibodies (1:800 dilution, all Biolegend). After labelling for 30 min at 4 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... and then incubated for one hour in PE anti-human CD71 Antibody in the dark at 4°C (1:100; Biolegend). Cells were washed twice with PBS and then and then analysed using a flow cytometer (FACsFortessa ...
-
bioRxiv - Cancer Biology 2023Quote: ... target tumor cells and effector NK cells were prepared as described above and one microgram of Anti-NKG2D (clone C7, BioLegend) or Armenian Hamster IgG isotype control (clone HTK888 ...
-
bioRxiv - Molecular Biology 2021Quote: ... using a LEGEND MAX™ Human IL-6 Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) Kit (BioLegend) with pre-coated plates ...
-
bioRxiv - Immunology 2022Quote: ... IL-1α and IL-6 were quantified with Mouse IFN-γ ELISA MAXTM Deluxe Set (Biolegend), Mouse IL-1α ELISA MAXTM Deluxe Set (Biolegend ...
-
bioRxiv - Cancer Biology 2022Quote: ... using ELISA MAX Deluxe Set human (IFNγ #430104, IL-2 # 431804, and IL-6 #430504, BioLegend) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... the samples were incubated with 4’,6-diamidino-2-phenylindole (DAPI) (422801, BioLegend, San Diego, CA) for 2 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... All three groups were co-treated with IL-6 (10 ng/mL, BioLegend cat no. 575706). We included one control group that was treated with DMSO ...
-
bioRxiv - Immunology 2023Quote: ... in the presence of Th17 polarizing cocktails containing recombinant mouse IL-6 (10 ng/ml, Biolegend), mouse IL-1β (10 ng/ml ...
-
bioRxiv - Molecular Biology 2021Quote: ... 11 Satb1 cKO) of varying age 1-7 months by the LEGENDplex (13-plex) Mouse Th Cytokine Panel V02 (Biolegend, 740741; Lot B289245) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: BMDMs were generated by culturing total BM cells for 7 days in complete RPMI supplemented with 10 ng/mL recombinant mouse M-CSF (BioLegend, Cat# 576406M-CSF). C8-S cells (ATCC ...
-
bioRxiv - Cancer Biology 2022Quote: ... Single cell RNAseq data for the 7 PDA cell lines were generated as following: Cell lines were trypsinized and resuspended in Cell Staining Buffer (Biolegend, Cat. No. 420201) and incubated with proper Total Seq B antibodies using recommended protocol from Biolegend ...
-
bioRxiv - Cell Biology 2022Quote: ... and the cells were resuspended in 200 μL biding buffer with 2μL 7-AAD and 2μL annexin V-FITC (Biolegend Inc, San Diego, CA, USA). The plate was incubated for 15 min in darkness and measured in the flow cytometer FC 500 (Beckman-Coulter ...
-
bioRxiv - Cancer Biology 2022Quote: ... The cells were expanded for one week and those that were double negative for MHC class I (Biolegend, W6/32, AF700) and β2 microglobulin (Biolegend ...
-
bioRxiv - Neuroscience 2022Quote: ... Multiplexing was performed by washing and hashing nuclei from each sample with one of six unique TotalSeq™-A anti-nuclear pore complex antibodies (BioLegend). Two multiplexed sequencing batches were then sequenced by the Oxford Genomics Centre (Wellcome Centre for Human Genetics ...
-
bioRxiv - Bioengineering 2022Quote: ... fluorescence minus one (FMO) + isotype control antibody staining was performed using the following isotypes: PE/Cy7 Mouse IgG2a κ (cat # 400232, BioLegend), BV-421 Mouse IgG1 κ (cat # 400158 ...
-
bioRxiv - Cell Biology 2022Quote: ... EGFR knockout cells were also validated by testing for loss of tyrosine phosphorylation in response to a one-minute treatment with 50 ng/mL mouse EGF by immunoblotting with mouse monoclonal antibody (PY20, BioLegend, 309301).