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Citations for BioLegend :
601 - 650 of 1074 citations for 6 METHYLAMINO 5 NITROQUINOXALINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... NPCs were we pelleted and washed once with AutoMACS buffer before resuspending AutoMACS with in DAPI (dead cell dye DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 422801, BioLegend) and DRAQ5 (DRAQ™ Live cell dye ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... CD3+ T cells were purified from C57BL/6 spleen cells with MojoSort mouse CD3 T cell isolation kit (Biolegend, USA), while CD8+T cells were isolated by adding biotin-labeled anti-CD4 antibody into MojoSort mouse CD3 T cell isolation kit ...
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bioRxiv - Physiology 2023Quote: ... IL-6 and IL-10 in the homogenates were measured using LEGENDplexTM Mouse Custom Panel (BioLegend, San Diego, CA, USA). Assay was performed in the Attune NxT flow cytometer (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... from uninfected or MCMV-infected WT C57BL/6 mice were sorted as described and stained with barcoded antibodies (Total-Seq B, BioLegend). After hash-staining ...
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bioRxiv - Immunology 2024Quote: ... derived from OT-1 mice treated with either CATR or vehicle were restimulated with 1µg/ml OVA-peptide for 6 hours and Brefeldin A (Biolegend, 420601) for 4hr prior to analysis.
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bioRxiv - Molecular Biology 2024Quote: IL-6 KO mice with cast immobilization for 24 h were subjected to intraperitoneal administration of 400 ng of rIL-6 (575702, BioLegend) in saline or of saline alone ...
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bioRxiv - Cancer Biology 2019Quote: ... at concentration 2×10^6/ml in IMDM containing 10% FBS and 100U/ml of recombinant mouse IL-2 (BioLegend, CA). Cells were cultured for up to 7 days and used for functional experiments in vitro and in vivo.
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bioRxiv - Microbiology 2021Quote: ... and resuspended in RPMI with 10% FBS medium and were stimulated for 6 h with a cell activation cocktail containing PMA and ionomycin (Biolegend, # 423302) in the presence of protein transport inhibitor ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Developmental Biology 2021Quote: ... Surface staining was done by adding the flow cytometry antibody cocktail outlined in Extended Data Table 6 containing F4/80 (BioLegend, 123120), CD11b (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were induced to differentiate by culturing in the absence of β-Estradiol for 6 days before cells were stained for CD11b (Biolegend) and analyzed on the LSRII flow cytometer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... Splenocytes from naive or tumor-bearing AST;Cre-ERT2 mice were mixed with 2×106 C57BL/6 splenocytes and incubated in RPMI-10 for 4 hours at 37°C in the presence of brefeldin A (Biolegend 420601) and TAG peptide (SAINNYAQKL [0.5 μM] ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently with biotinylated α- CADM1 antibody (MBL, Cat. No. CM004-6) and streptavidin-conjugated secondary antibody (BioLegend, Cat. No. 405226). Cells were resuspended in 4◦C PBS 2% FCS before cell sorting.
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bioRxiv - Cancer Biology 2023Quote: For inflammatory activation colon fibroblasts were plated in 6-wells and after 24 hours treated with 1 ng/ml murine recombinant IL-1α (Biolegend, 575002) in PBS for the indicated time periods ...
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bioRxiv - Cancer Biology 2023Quote: Plasma Ig were analysed using the LEGENDplex Mouse Immunoglobulin Isotyping panel (6-plex) by flow cytometry (Cat #740493, BioLegend, CA, USA). Plasma sample and standards was prepared using assay buffer as per the manufacturer’s instruction ...
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bioRxiv - Physiology 2023Quote: The levels of TNF-α and IL-6 in the serum and the supernatant of the crushed muscle tissues were measured using a commercial kit (mouse TNF-α and IL-6 ELISA max standard set, BioLegend). The values of TA and GA muscles were revised to the protein concentration measured by BCA kit and expressed as pg/mg.
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... Serum IL-6 and TNF-α concentrations were determined with enzyme-linked immunosorbent assay (ELISA) kits (Legend Max Mouse IL-6 ELISA Kit, BioLegend, and Mouse TNF Alpha Uncoated ELISA ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used: CD4 (Clone RM4-5, BioLegend, Cat 100527), GMCSF (Clone MP1-22E9 ...
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bioRxiv - Immunology 2019Quote: ... cells were stained with biotin-conjugated anti-CD2 (clone RM2-5, BioLegend) antibody and negative selection was performed by using an EasySep Biotin Selection Kit (Stemcell Technologies ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated with 5 μL APC Annexin V (Biolegend: 640920) and 10 μL PI solution (Biolegend ...
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bioRxiv - Bioengineering 2022Quote: ... 5 μL of 25 μg/mL anti-CD4 PE (Biolegend, Catalog: 344606) and 5 μL of 100 μg/mL anti-CD8 Alexa Fluor 488 (Biolegend ...
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bioRxiv - Bioengineering 2022Quote: ... and 5 μg/mL of CD40 antibody ligand (clone: HM40-3; BioLegend).
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bioRxiv - Immunology 2022Quote: ... Cells were treated with 5 µg/mL of Brefeldin A (BioLegend, 420601) and allowed to incubate for 6 hours in a 5% CO2 ...
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bioRxiv - Immunology 2019Quote: ... TNFα was blocked with 5 µg/mL anti-TNFα antibody (Mab11, BioLegend); IgG1 isotype control (MOPC-21 ...
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bioRxiv - Cancer Biology 2019Quote: ... Human CD96 blocking antibody (5 μg/ml) or isotype control (Biolegend, USA) was included in some experiments.
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bioRxiv - Genetics 2020Quote: ... We stimulated Jurkat cells with 5 μg/ml anti-CD3 (Biolegend-317315) and 100 ng/ml phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Neuroscience 2021Quote: ... and Rat IgG2a,k AF647 Isotype Control (RTK2758, 5 μg/mL, Biolegend).
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bioRxiv - Immunology 2021Quote: ... in the presence of plate- bound anti-CD3 (5 μg/mL, Biolegend), CpG (10 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... CD4 (RM4-5; BV 711) and PD-1 (J43; FITC; all BioLegend). After fixation and permeabilization (Foxp3 Transcription Factor Staining Buffer Set ...
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bioRxiv - Immunology 2022Quote: ... and blocked with 5 μg/mL anti-CD16/32 (clone 93; Biolegend) and heat inactivated normal mouse serum (1:10 ...
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bioRxiv - Immunology 2022Quote: ... single cell suspensions were incubated for 5 hours in 2μM monensin (BioLegend) to inhibit intracellular protein trafficking ...
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bioRxiv - Cancer Biology 2022Quote: ... and stimulated with 5 ug/mL Ultra-LEAF anti-human CD3 (Biolegend) and 2.5 ug/mL Ultra-LEAF anti-human CD28 (Biolegend) ...
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bioRxiv - Neuroscience 2024Quote: ... CD64-PE-594 (BioLegend, clone X54-5/7.1, cat #139319, 1:400), Ly6c-BV711 (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... keratin 5 (1:2000, KRT5 for basal cells; BioLegend, Catalog number: 905503), SCGB1A1 (1:200 ...
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bioRxiv - Molecular Biology 2024Quote: ... Brilliant Violet 605 anti-mouse CD4 (Biolegend, Cat# 100548, Clone RM4-5), Fluorescein isothiocyanate (FITC ...
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bioRxiv - Microbiology 2024Quote: ... 5 µl of APC anti-human Ki-67 Antibody (BioLegend, CAT# 350513) in 100 µL of cell solution ...
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bioRxiv - Immunology 2024Quote: ... anti-CD64 (X-54-5/7.1, Biolegend 139323 or BD Biosciences 558539), anti-MERTK (DS5MMER ...
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bioRxiv - Immunology 2024Quote: ... the cells were incubated with 5 μg/mL brefeldin A (#420601, BioLegend), 20 μM monensin (#420701 ...
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bioRxiv - Immunology 2024Quote: ... Samples were then blocked with 5 μg/ml αCD16/CD32 (2.4G2; BioLegend) in FACS buffer (PBS containing 2% FBS and 2 mM EDTA ...
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bioRxiv - Immunology 2023Quote: ... wells were pre-coated with 5 μg/mL ICAM-1 (Biolegend, #553006) for 2-3 hours at 37°C before being emptied for seeding ...
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bioRxiv - Microbiology 2023Quote: ... CD4 (mouse, FITC fluorochrome, clone RM4-5, BioLegend 100510, 1:200 dilution), CD8a (mouse ...
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bioRxiv - Cell Biology 2022Quote: ... anti-CD64-PE/Dazzle594 (1:50, clone X54-5/7.1, Biolegend, 139320), anti-CD11b-PE (1:100 ...
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bioRxiv - Immunology 2023Quote: ... for 5-7h in the presence of Brefeldin A (Biolegend, Cat#420601) in a 96-well plate ...
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bioRxiv - Bioengineering 2024Quote: ... NPC pellet was resuspended in 5 mL RBC lysis buffer (BioLegend, 420301) and incubated for 5 min at 4 ℃ ...
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bioRxiv - Molecular Biology 2024Quote: ... and anti-mouse CD40 antibody (5 µg ml-1; HM40-3, Biolegend), or anti-IgM F(ab’)₂ fragment (5 µg ml-1 ...
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bioRxiv - Immunology 2020Quote: ... KC and IL-6 in the cell culture supernatants and lung homogenates were determined by commercialized ELISA kit (IL-1β and KC were from R&D Systems BD, IL-6 was from Biolegend) and SAA3 in the lung homogenates and plasma were determined by ELISA kit (MilliporeSigma ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular P-STAT5 staining was then performed in PBS with wash steps performed at 1800 rpm for 6 min at 4°C.The following antibody clones and fluorochromes were used: CD3 (UCHT1, Biolegend; Bv510, Bv711, FITC), CD8 (SK1 ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4+ T cells were isolated from the spleens of C57BL/6 mice or IL17-GFP mice using a MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (BioLegend cat. 480040). T cells from C57BL/6 mice were further labeled with CellTrace™ Violet (Invitrogen cat ...