3 3 2 3 Dimethyl indol 1 yl 2 hydroxy propylamino propan 1 ol citations for BioLegend

Quote: ... and interleukin-2 (IL-2) and interferon-ψ concentrations were determined by enzyme-linked immunosorbent assay (BioLegend). Triplicate wells were plated for each condition.

Evolutionary fingerprint in rodent PD1 confers weakened activity and enhanced tumor immunity compared to human PD1

Takeya Masubuchi, et al., bioRxiv - Immunology 2024

Quote: ... Secreted IL-2 in the supernatant was measured using Human or Mouse IL-2 ELISA Kit (BioLegend). All incubations were performed at 37 °C / 5% CO2.

Evolutionary fingerprint in rodent PD1 confers weakened activity and enhanced tumor immunity compared to human PD1

Takeya Masubuchi, et al., bioRxiv - Immunology 2024

Quote: ... the amount of IL-2 in the medium was quantified using Human IL-2 ELISA kit (Biolegend) or CD69 expression on Jurkat cells was measured by FACS ...

Targets of influenza Human T cell response are mostly conserved in H5N1

John Sidney, et al., bioRxiv - Immunology 2024

Quote: ... while CD137 APC (2:100) and OX40 PE-Cy7 (2:100; Biolegend; clone Ber-ACT35; Cat# 350012) were used for CD4+ T cells.

Functional single-cell profiling identifies that exosomes are associated with increased immune cell infiltration in non-metastatic breast cancer

Mohsen Fathi, et al., bioRxiv - Cancer Biology 2020

Quote: ... catalog number L100-L115-01) in PBS with 1% BSA and incubated the beads with 3.5 µg/ml biotinylated anti-CD81 antibody (BioLegend, clone Eat-2) at the room temperature for 40 minutes ...

Genetic and compound screens uncover factors modulating cancer cell response to indisulam

Ziva Pogacar, et al., bioRxiv - Cancer Biology 2021

Quote: ... 10 μL of CytoC stain buffer (2% Tween20, 10% BSA (w/v) in PBS) + 1:400 Alexa Fluor 647 anti-cytochrome c antibody (Biolegend, cat#612310) + 1:100 DAPI (1 mg/mL ...

SARS-CoV-2 Omicron boosting induces de novo B cell response in humans

Wafaa B. Alsoussi, et al., bioRxiv - Immunology 2022

Quote: ... lymph node FNA samples collected 2 or 8 weeks post-boost were stained for 30 min on ice with purified CD16 (3G8, BioLegend, 1:100), CD32 (FUN-2 ...

Engineering the microstructure and spatial bioactivity of MAP scaffolds in vitro instructs neovascularization in vivo

Alexa R. Anderson, et al., bioRxiv - Bioengineering 2023

Quote: ... The slides were then incubated in the primary antibodies (rat anti-PECAM-1, clone 390, EMD Millipore; rabbit anti-NG-2 chondroitin sulfate, EMD Millipore; rabbit anti-Keratin-14, BioLegend) diluted 1:100 in the blocking solution overnight at 4 °C ...

Memory B cells dominate the early antibody-secreting cell response to SARS-CoV-2 mRNA vaccination in naïve individuals independently of their antibody affinity

Zhe Li, et al., bioRxiv - Immunology 2023

Quote: ... Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen ...

On-chip human lymph node stromal network for evaluating dendritic cell and T-cell trafficking

Brian J. Kwee, Adovi Akue, Kyung E. Sung, bioRxiv - Bioengineering 2023

Quote: ... and stained with the following antibodies in FACS staining buffer (PBS with 2% Fetal Bovine Serum, 1% Penicillin-Streptomycin): anti-human CD31 (BioLegend, WM59, FITC), anti-human CD45 (BioLegend ...

ATG5 suppresses type I IFN-dependent neutrophil swarming and NETosis

Rachel L. Kinsella, et al., bioRxiv - Immunology 2023

Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...

Conserved epigenetic programming and enhanced heme metabolism drive memory B cell reactivation

Madeline J. Price, et al., bioRxiv - Immunology 2021

Quote: ... anti-IgD-APC-Cy7 (Biolegend, IA6-2), anti-CD38-PE-Cy7 (Biolegend ...

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