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6351 - 6400 of 8552 citations for 5 Pyrimidinecarbonitrile 1 2R 2 3 dihydroxypropyl 1 2 3 4 tetrahydro 3 methyl 2 4 dioxo 9CI since 2019

• EOGT Enables Residual Notch Signaling in Mouse Intestinal Cells Lacking POFUT1

  Mohd Nauman, et al., bioRxiv - Developmental Biology 2023

  Quote: ... pelleted and resuspended in Zombie NIR dye in PBS (1:7000; Biolegend). After 30 minutes rocking at 4°C ...
• Type I IFN signaling mediates NET release to promote Mycobacterium tuberculosis replication and granuloma caseation

  Chanchal Sur Chowdhury, et al., bioRxiv - Microbiology 2022

  Quote: ... neutrophils on cover slips were treated with Zombie NIR (BioLegend, 1:1000) for 5 min before PFA fixation and staining with Hoechst and other antibodies ...
• Fate and plasticity of SARS-CoV-2-specific B cells during memory and recall response in humans

  Yves Zurbuchen, et al., bioRxiv - Immunology 2022

  Quote: ... They were then stained with ZombieUV Live-Dead staining (1:400, Biolegend) and TruStain FcX (1:200 ...
• Reduction of aggressive behaviour following hypothalamic deep brain stimulation: involvement of 5-HT_1A and testosterone

  Flavia Venetucci Gouveia, et al., bioRxiv - Neuroscience 2023

  Quote: ... and anti-tubulin-β3 loading control (BioLegend #801202, mouse mAb, 1:2000). Membranes were then incubated with HRP-linked secondary antibodies (CST #7074 ...
• An interwoven network of transcription factors, with divergent influences from FoxP3, underlies Treg diversity

  Kaitavjeet Chowdhary, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were washed and stained with pSTAT5 (Tyr694) antibody (1:20, BioLegend) at room temperature for 40 minutes ...
• Ankyrin B Promotes Developmental Spine Regulation in the Mouse Prefrontal Cortex

  Kelsey E. Murphy, et al., bioRxiv - Neuroscience 2023

  Quote: ... Also used were antibodies to vesicular glutamate transporter-1 (vGlut1, #821301, Biolegend) and Homer-1 (#160002 ...
• Two birds with one stone: human SIRPα nanobodies for functional modulation and in vivo imaging of myeloid cells

  Teresa R. Wagner, et al., bioRxiv - Immunology 2023

  Quote: ... DLD-1 cells were labeled with CFSE Cell Division Tracker Kit (BioLegend) according to manufacturer’s instructions ...
• Antigen presentation by tumor-associated macrophages mediates progenitor to terminal exhaustion transition in GBM and other solid tumors

  Jessica Waibl Polania, et al., bioRxiv - Immunology 2023

  Quote: ... samples were resuspended in 50 µl TruStain FcX Plus (1:500, Biolegend) in FACS (PBS + 2% FBS ...
• Phosphoprotein dynamics of interacting tumor and T cells by HySic

  Sofía Ibáñez-Molero, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Cells were then stained with CFSE (1:6000 in PBS, Biolegend, 423801) for 20 min at 37°C and washed with 2% BSA in FBS ...
• Glioma-neuronal circuit remodeling induces regional immunosuppression

  Takahide Nejo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and 1% TruStain FcX PLUS anti-mouse CD16/32 Antibody (BioLegend, 156604) for 40 min at RT ...
• Phosphoprotein dynamics of interacting tumor and T cells by HySic

  Sofía Ibáñez-Molero, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The following antibodies were used: CXCL-9-APC (1:20, Biolegend, 357906), PD-L1/CD274-PECy7 (1:50 ...
• Acquisition of drug resistance in basal cell nevus syndrome tumors through basal to squamous cell carcinoma transition

  Anna R. Jussila, et al., bioRxiv - Cancer Biology 2023

  Quote: ... we used the chicken K14 antibody (1:500; BioLegend SIG-3476-100) with the anti-chicken Alexa647 (1:500 ...
• PARP12 is required to repress the replication of a Mac1 mutant coronavirus in a cell and tissue specific manner

  Catherine M. Kerr, et al., bioRxiv - Microbiology 2023

  Quote: ... MHC I (H-2Kb/Db) (FITC, Biolegend, 1:200, clone 28.8-6), Ly6C (BV785 ...
• Targeting pioneer transcription factor Ascl1 to promote optic nerve regeneration

  Bryan C Dong, et al., bioRxiv - Neuroscience 2023

  Quote: ... stained with primary antibodies overnight (1:500 mouse anti-Tubulin β3, BioLegend; 1:500 rabbit anti-Sox11 ...
• Androgen exposure impairs neutrophil maturation and function within the infected kidney

  Teri N. Hreha, et al., bioRxiv - Immunology 2023

  Quote: ... CD49b-PE/Dazzle 594 (1:50, for C3H/HeN mice, Biolegend #108924), NK1.1-PE/Dazzle 594 (1:400 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mPD-1 (RPM1-30, BioLegend, 109110, or 29F.1A12, BioLegend, 135218), anti-mCD44 (IM7 ...
• Autoimmunity associated allele of tyrosine phosphatase gene PTPN22 enhances anti-viral immunity

  Robin C. Orozco, et al., bioRxiv - Immunology 2023

  Quote: ... PD- L1 (PE/BV711, Tonbo Biosciences/Biolegend, 1:100, clone 10F.9G2), CD44 (AF700 ...
• PVN microglia via P2Y₁₂ transmit hemodynamic signal to promote sympathetic excitation in hypertension

  Bo Wei, et al., bioRxiv - Neuroscience 2023

  Quote: ... an Alexa488-conjugated anti-BrdU antibody (1:500, Biolegend, USA, Catalog #364105) was used.
• Microenvironmental Snail1 is a driver of immunosuppression in melanoma

  Marta Arumi-Planas, et al., bioRxiv - Cancer Biology 2022

  Quote: ... samples were blocked with Fc-block CD16/CD32 (Biolegend, 101320, 1:50) in FACS buffer for 10 min on ice to block nonspecific binding ...
• Palmitoyl transferase ZDHHC20 promotes pancreatic cancer metastasis

  Goran Tomić, et al., bioRxiv - Cancer Biology 2023

  Quote: ... followed with the addition of anti-CD45 antibody (1:600, PerCP, Biolegend) and Nkp46 antibody (1:40 ...
• Distinct tumor architectures for metastatic colonization of the brain

  Siting Gan, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 1:100 human TruStain FcX (Fc Receptor Blocking Solution) (BioLegend, Cat: 422301) and 1:100 TruStain FcX (anti-mouse CD16/32 ...
• Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies

  Katrin Fischer, et al., bioRxiv - Immunology 2023

  Quote: ... and anti-FLAG Alexa Fluor 594 (1:200 dilution, clone L5, BioLegend). The sample was incubated on ice for 30 min and washed once in FACS buffer ...
• Single-dose YF17D-vectored Ebola vaccine candidate protects mice against both lethal surrogate Ebola and yellow fever virus challenge

  Viktor Lemmens, et al., bioRxiv - Immunology 2023

  Quote: ... and APC/Cyanine7 anti-CD8 (1:100 dilution, clone 53-6.7, Biolegend) in Brilliant Staining Buffer (BD Biosciences ...
• Secretory IgM (sIgM) is an ancient master regulator of microbiota homeostasis and metabolism

  Yang Ding, et al., bioRxiv - Immunology 2023

  Quote: ... The stained cells were detected with streptavidin-BV421 (1 μg/ml; Biolegend) and goat anti-mouse IgG2b-PE pAbs (1 μg/ml ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... cells were incubated with Alexa Fluor 594 streptavidin (1:500, 405240; BioLegend) for an additional 30 min ...
• Isthmus progenitor cells contribute to homeostatic cellular turnover and support regeneration following intestinal injury

  E. Malagola, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Cells were stained for 20 minutes Epcam-APC (1:200, BioLegend 118214), washed and re-suspended in media containing DAPI (1:10’000 ...
• Dynamic mitochondrial transcription and translation in B cells control germinal centre entry and lymphomagenesis

  Yavuz F Yazicioglu, et al., bioRxiv - Immunology 2023

  Quote: ... and PE-conjugated Donkey anti-rabbit IgG (1:200, Biolegend, cat: 406421). Following wash with Perm/Wash buffer ...
• The Sequence Basis for Selectivity of Ephrin-B2 Ligand for Eph Receptors and Pathogenic Henipavirus G Glycoproteins

  Krishna K. Narayanan, et al., bioRxiv - Microbiology 2023

  Quote: ... and anti-human IgG-allophycocyanin (APC) (clone M1310G05, 1/250 dilution, BioLegend) in PBS-BSA for 20 minutes at 4°C ...
• Allogeneic Tumor Cell-Derived Extracellular Vesicles Stimulate CD8 T Cell Response in Colorectal Cancer

  Travis J Gates, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Antibodies in flow cytometry were CD3-FITC (cat#100204, Biolegend, 1:100), CD19FITC (cat#115506 ...
• Allogeneic Tumor Cell-Derived Extracellular Vesicles Stimulate CD8 T Cell Response in Colorectal Cancer

  Travis J Gates, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Antibodies for flow cytometry were CD3-APC (cat# 100236, Biolegend, 1:200) CD8-APCCy7 (cat# 100714 ...
• A rationally designed inactivated Salmonella Typhimurium vaccine induces strong and long-lasting immune responses in pigs

  Verena Lentsch, et al., bioRxiv - Immunology 2023

  Quote: ... and Brilliant Violet 421-anti-rabbit IgG (BioLegend, 1:100, 406410, AB_10897810)) ...
• Intestinal transit amplifying cells require METTL3 for growth factor signaling, KRAS expression, and cell survival

  Charles H. Danan, et al., bioRxiv - Cell Biology 2023

  Quote: ... Cell suspensions were then stained with 1:200 PE-EpCAM (BioLegend 118205), 1:200 FITC-CD45 (BioLegend 103107) ...
• In vivo screening of tumor-hepatocyte interactions identifies Plexin B2 as a gatekeeper of liver metastasis

  Costanza Borrelli, et al., bioRxiv - Cancer Biology 2023

  Quote: ... TruStain FcX™ (anti-mouse CD16/32) antibody (BioLegend #101320, 1:50), PE/Cy7 anti-mouse CD31 (BioLegend #102418 ...
• Loss of IRF5 increases ribosome biogenesis leading to alterations in mammary gland architecture and metastasis

  Zarina Brune, et al., bioRxiv - Cancer Biology 2023

  Quote: FITC rat anti-mouse CD45 (Biolegend, #103108, 0.5 mg/ml, 1:800)
• Loss of IRF5 increases ribosome biogenesis leading to alterations in mammary gland architecture and metastasis

  Zarina Brune, et al., bioRxiv - Cancer Biology 2023

  Quote: PE/Cy5 anti-mouse CD24 (Biolegend, #101812, 0.2 mg/ml, 1:250)
• Loss of IRF5 increases ribosome biogenesis leading to alterations in mammary gland architecture and metastasis

  Zarina Brune, et al., bioRxiv - Cancer Biology 2023

  Quote: FITC anti-mouse/rat CD61 (Biolegend, #104306, 0.5 mg/mL, 1:100)
• Loss of IRF5 increases ribosome biogenesis leading to alterations in mammary gland architecture and metastasis

  Zarina Brune, et al., bioRxiv - Cancer Biology 2023

  Quote: APC rat anti-mouse CD24 (Biolegend, #101814, 0.2 mg/ml, 1:250)
• Loss of IRF5 increases ribosome biogenesis leading to alterations in mammary gland architecture and metastasis

  Zarina Brune, et al., bioRxiv - Cancer Biology 2023

  Quote: Alexa Fluor® 647 anti-human/mouse CD49f (Biolegend, #313609, 1:100)
• Androgen exposure impairs neutrophil maturation and function within the infected kidney

  Teri N. Hreha, et al., bioRxiv - Immunology 2023

  Quote: ... NK1.1-PE/Dazzle 594 (1:400, for C57BL/6 mice, Biolegend #108748), Ly6C-PE/Cy5.5 (1:200 ...
• Engineered CD47 protects T cells for enhanced antitumor immunity

  Sean A. Yamada-Hunter, et al., bioRxiv - Bioengineering 2023

  Quote: ... NY-ESO-1 TCR was detected with antibodies specific for Vβ13.1 (BioLegend), the beta chain of the NY-ESO-1 TCR ...
• Selective inhibition of fibroblast-specific Domain Discoidin Receptor 1 (DDR1) reduces collagen deposition and modulates fibroblast-specific cytokine release within the breast microenvironment

  Daniel E.C. Fein, et al., bioRxiv - Cell Biology 2023

  Quote: ... the following antibodies were used: CD45.2-APC-Cy7 (BioLegend 109823; 1:200), CD11b-PE (BioLegend 101207 ...
• Targeting CD74-positive macrophages improves neoadjuvant therapy in cervical cancer as revealed by single-cell transcriptomics analysis

  Zixiang Wang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and PE anti-mouse CD279 (PD-1) Antibody (Biolegend, USA, Cat#135206). CD45 antibody is used to screen monocytes in cell suspension ...
• MarrowDLD: a microfluidic method for label-free retrieval of fragile bone marrow cells

  Gloria Porro, et al., bioRxiv - Bioengineering 2023

  Quote: ... and CD42b (PE, clone HIP1, Biolegend, Cat. No. 303906, Dilution 1:200) surface detection ...
• Heterotypic responses against nsp12/nsp13 from prior SARS-CoV-2 infection associates with lower subsequent endemic coronavirus incidence

  David J. Bean, et al., bioRxiv - Immunology 2023

  Quote: ... Brilliant Violet 421 anti-human CD134 (OX40) (1:25 dilution, BioLegend, 356147), PE anti-human CD137 (4-1BB (1:100 dilution ...
• Heterotypic responses against nsp12/nsp13 from prior SARS-CoV-2 infection associates with lower subsequent endemic coronavirus incidence

  David J. Bean, et al., bioRxiv - Immunology 2023

  Quote: ... APC/Fire™ 750 anti-human CD8a (1:50 dilution, BioLegend, 300931), PE/Cyanine7 anti-human CD69 (1:50 dilution ...
• Myeloid A20 is critical for type-2 immune mediated helminth resistance

  Ioanna Petta, et al., bioRxiv - Immunology 2023

  Quote: ... CD3 (eBioscience; 15-0031-82; 1/200) and NK1.1 (BioLegend; 108716;1/200) PE-Cy5 ...
• Myeloid A20 is critical for type-2 immune mediated helminth resistance

  Ioanna Petta, et al., bioRxiv - Immunology 2023

  Quote: ... CD3 (eBioscience; 15-0031-82; 1/200) and NK1.1 (BioLegend; 108716;1/200) PE-Cy5) ...
• Matrix architecture and mechanics regulate myofibril organization, costamere assembly, and contractility of engineered myocardial microtissues

  Samuel J. DePalma, et al., bioRxiv - Bioengineering 2023

  Quote: ... rabbit polyclonal anti-non-muscle myosin II-B (1:1000; Biolegend 909902), rabbit monoclonal anti-paxillin (1:500 ...
• Short-range interactions between fibrocytes and CD8⁺ T cells in COPD bronchial inflammatory response

  Edmée Eyraud, et al., bioRxiv - Pathology 2023

  Quote: ... the antibodies (Abs) directed against LFA-1 (clone HI111, BioLegend, 1µg/mL), CD54 (clone HA58 ...
• Multi-parameter phenotyping of platelets and characterisation of the effects of agonists using machine learning

  Ami Vadgama, et al., bioRxiv - Immunology 2023

  Quote: ... an antibody mix comprising anti-CD42b-BV421 (1:70; clone HIP1; BioLegend), PAC-1-FITC (1:10 ...