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Citations for BioLegend :
6351 - 6400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: The antibodies used for flow cytometry are Alexa Fluor 647 mouse anti-human BST2 (Biolegend; 348404) and HIV-1 core antigen-FITC (KC57 ...
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bioRxiv - Microbiology 2023Quote: ... from Biolegend) ...
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bioRxiv - Microbiology 2023Quote: ... PE/Cyanine7 anti-mouse CD45 Antibody (103114, BioLegend), APC anti-mouse TNF-α Antibody (506307 ...
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bioRxiv - Microbiology 2023Quote: ... APC anti-mouse TNF-α Antibody (506307, BioLegend), FITC anti-mouse IL-6 Monoclonal Antibody (MP5-20F3 ...
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bioRxiv - Microbiology 2023Quote: ... from Biolegend) ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently with biotinylated α- CADM1 antibody (MBL, Cat. No. CM004-6) and streptavidin-conjugated secondary antibody (BioLegend, Cat. No. 405226). Cells were resuspended in 4◦C PBS 2% FCS before cell sorting.
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bioRxiv - Microbiology 2023Quote: ... The following antibodies were used for flow cytometry analysis: FITC-conjugated anti-CD19 (6D5) (BioLegend, San Diego, CA, USA, cat# 115505), APC-conjugated anti-CD11b (M1/70 ...
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bioRxiv - Microbiology 2023Quote: ... IFNγ-treatment was performed with 1000 U/ml (BioLegend) for 16 hours ...
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bioRxiv - Microbiology 2023Quote: ... PE/Cy7-coupled anti-TNFα (BioLegend, 506323, AB_2204356), HRP-coupled goat anti-mouse IgG (Dianova ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed and stained with a viability dye (Zombie Aqua Fixable viability kit, BioLegend) and fixed with 100µl of pre-warmed Cytofix Fixation Buffer (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... and washed once with 1x Perm/Wash Buffer (PWB, BioLegend). Cells were incubated for 30 min at 37°C with the intracellular antibody cocktail in PWB ...
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bioRxiv - Microbiology 2023Quote: ... Total cell counts per mouse were calculated using Precision Count Beads (Biolegend, 424902) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... and Ly6G-BV421 (Biolegend,127628), CD11b-Alexa700 (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... for 5 minutes at 4°C and then fixed using commercial fixation buffer for 3 minutes (ref no. 420801, BioLegend). For intracellular staining of Granzyme B and Perforin ...
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bioRxiv - Microbiology 2023Quote: ... island Avidin-HRP (BioLegend) were diluted 1:200 and 1:500 ...
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bioRxiv - Microbiology 2023Quote: ... 1X Brefeldin-A (BFA solution 1000X, ref no.420601, BioLegend) was added into each well to block secretion of IFNγ for 4 hours ...
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bioRxiv - Microbiology 2023Quote: ... single cell suspensions are incubated with 1X Brefeldin-A for 4 hours at 37°C to block secretion of cytokines (ref no.420601, BioLegend). For all ...
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bioRxiv - Microbiology 2023Quote: ... Secreted levels of IFN-β were measured from BMDM supernatants twenty-four hours following the start of the infection following the Sandwich ELISA protocol published by BioLegend. Anti-IFN-β capture antibody (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... Anti-IFN-β capture antibody (BioLegend, SanDiego, CA) was diluted 1:200 in Carbonate Coating Buffer [0.84% w/v NaHCO3 (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... while the detection antibody (Biotin anti-mouse IFN-β; BioLegend) island Avidin-HRP (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed once with cold HMI-9 and resuspended in 200μl cold HMI-9 with rat anti-mouse IgM-FITC (1:500, Biolegend). After one wash with cold HMI-9 ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then stained for 30 min with CD45-BV510 (Biolegend, 103138) and Ly6G-BV421 (Biolegend,127628) ...
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bioRxiv - Microbiology 2023Quote: ... lysed in 1X red blood cell lysis buffer for 3 minutes (ref no. 420301, BioLegend), and passed successively through 70μm and 30μm strainers (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (CD45+ immune cells: 1:50 anti-CD45-VioGreen [Miltenyi Biotec, Germany]; CD31+ endothelial cells: 1:2000 anti-CD31-PE [BioLegend, CA]/1:50 anti-CD45-VioGreen [Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was incubated in 1% milk-PBS-T containing rabbit anti-LpxC antibody (a generous gift from the Doerrler lab) or mouse anti-RpoA (anti-E.coli RNA polymerase alpha from Biolegend, cat# 663104) diluted 1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... following manufacturer recommendations using a custom LEGENDplex™ (Biolegend) bead-based immunoassay for the following cytokines ...
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bioRxiv - Immunology 2023Quote: ... followed by treatment with RBC lysis buffer (Biolegend, Cat# 420301). BAL cells and single lung cells were first gated by (FSC-A x SSC-A ...
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bioRxiv - Immunology 2023Quote: ... Surfaced staining was conducted using Cell Staining Buffer (Biolegend, Cat# 420201). Intracellular and nuclear staining was carried out with eBioscience™ Intracellular Fixation & Permeabilization Buffer (Cat# 88-8823-88 ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... precision counting beads (BioLegend, San Diego, CA) were added to each sample before flow cytometry analysis ...
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bioRxiv - Immunology 2023Quote: ... Flow cytometry antibodies are from Biolegend [Alexa Fluor® 700 anti-mouse CD45 (30-F11) ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were obtained from Biolegend, Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were purchased from BioLegend (San Diego, CA, USA). The staining took 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were obtained from Biolegend, Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Staining of intracellular BCL2 was performed with True-Nuclear™ Transcription Factor Buffer Set (Biolegend) as per manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were first stained with viability stain (Zombie NIR fixable viability kit, BioLegend, San Diego, California, USA) followed by either BV510-αCD45 (clone ...
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bioRxiv - Immunology 2023Quote: ... Brefeldin A (#420601, BioLegend) were used as inhibitors.
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bioRxiv - Immunology 2023Quote: ... anti-IL-6 (clone MP5-20F3, BioLegend), and isotype control (#RTK2071 ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated from the spleen using Mojosort Magnetic Separation System CD8 Naïve T cell Isolation Kit (BioLegend). Anti-CD3ε Ab (clone 145-2111C ...
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bioRxiv - Immunology 2023Quote: ... All T cell populations were first enriched by positive selection with CD4 MojoSort™ beads (Biolegend) before FACS sorting.
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bioRxiv - Immunology 2023Quote: ... and isotype control (#RTK2071, BioLegend) Abs were used ...
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bioRxiv - Immunology 2023Quote: ... anti-IL-23p19 (clone MMp19B2, BioLegend), anti-IL-6 (clone MP5-20F3 ...
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bioRxiv - Immunology 2023Quote: ... Supernatant were collected and measured by ELISA kits according to the manufacturer’s instructions (catalog 438707, 440507, 431414; BioLegend). Absorbance was measured at 450 nm with a microplate reader (Molecular Devices).
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bioRxiv - Immunology 2023Quote: ... and ELISA MAX Deluxe Set mouse TNF-α (BioLegend), respectively ...
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bioRxiv - Immunology 2023Quote: ... brefeldin (Biolegend) and monensin (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... One sample received an irrelevant antibody (a mouse IgG1k against IFNγ from Biolegend) control ...
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bioRxiv - Immunology 2023Quote: ... the culture supernatants were evaluated using the ELISA MAX assay kit (BioLegend), according to the manufacturer’s specifications.
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bioRxiv - Immunology 2023Quote: ... CD138-PE/Cyanine7 (Syndecan-1, clone 281-2, BioLegend), CD27-APC (clone LG.3A10 ...
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bioRxiv - Immunology 2023Quote: ... IgM-APC/Cyanine7 (clone RMM-1, BioLegend), CD86-BV510 (clone GL1 ...
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bioRxiv - Immunology 2023Quote: ... and anti-CD49b antibodies (clone HMa2, BioLegend; Alexa 488), respectively ...
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bioRxiv - Immunology 2023Quote: ... 5 μg of fluorescently conjugated anti-CD31 (clone 390, BioLegend; either Alexa 594) and anti-CD49b antibodies (clone HMa2 ...