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Citations for BioLegend :
6201 - 6250 of 7947 citations for Rat Caprin 1 CAPRIN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were isolated using the Mojosort Mouse CD4 Naïve T Cell Isolation Kit (Biolegend, San Diego, CA) using LS columns on a QuadroMACS magnet (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... 5×104 Tconv (CD4+CD25-) cells from TFAM control were labeled with CFSE Cell Division Tracker Kit (Cat# 423801, BioLegend) and cultured with mitomycin-treated wild type splenocytes ...
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bioRxiv - Neuroscience 2022Quote: ... CD4+ T cells were isolated from the spleens and draining lymph nodes of 2D2 mice using the CD4+ isolation kit (BioLegend) and co-cultured with irradiated wild-type splenocytes at a ratio of 1:5 ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were analyzed by using either the Legendplex Multi-Analyte Flow Assay Kit for human CD8/NK cells or the human Th cytokine panel (Biolegend).
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bioRxiv - Microbiology 2020Quote: To assess cell viability human neutrophils at indicated times were washed with PBS and incubated with Zombie Aqua Fixable Viability Kit (BioLegend) for 15 min at RT ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... About 0.3 million cells in 37.5 μl CSM were loaded in each well of a 96-well plate of the Human Cell Surface Marker Screening (phycoerythrin [PE]) Kit (Biolegend). Each well contained 12.5 μl of diluted PE-conjugated antibody in CSM ...
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bioRxiv - Immunology 2021Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed with PBS and stained with Zombie Aqua fixable live-dead kit as per supplier’s instructions (Biolegend, 423101) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Immunology 2020Quote: ... The percentage of apoptotic and necrotic cells were then determined by flow cytometry using the FITC Annexin V apoptosis detection kit with PI (BioLegend) following manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... and either directly fixed for ChIP or stained for dead cells using the Zombi NIR Fixable Viability Kit (Biolegend: #423106) as per the manufacturer’s instructions for subsequent FACS sorting (MHV-68-H2BYFP) ...
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bioRxiv - Cancer Biology 2020Quote: ... washed two times with ice cold PBS and processed for staining using FITC Annexin V Apoptosis Detection Kit with 7AAD (Cat # 640922, BioLegend), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD4 or CD8 T cell magnetic bead negative selection using the MojoSort Human CD4 or CD8 Selection Kit (BioLegend). Isolated CD4 or CD8 T cells were labeled with CellTrace Violet (CTV ...
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bioRxiv - Microbiology 2021Quote: ... Resting CD4+ T cells were isolated from total PBMCs by negative selection using the MojoSort Human CD4+ T Cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then labeled according to the manual of the FITC ANNEXIN V Apoptosis Detection Kit with 7-AAD (BioLegend) with a modification where the amount of 7-AAD was doubled.
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bioRxiv - Immunology 2021Quote: ... lymphocytes were stained in 100 μl PBS with a viability dye (Zombie Aqua™ Fixable Viability Kit, Biolegend, the Netherlands) for 15 min at RT in the dark ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were then isolated from the spleens using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend), following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2022Quote: 3×106 cells from each tonsil donor (n=3) at each time point were stained with the Zombie Red fixable viability kit (Biolegend) for 10 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... or P14 bone marrow cells from 2-4 mice per genotype were c-kit selected and stained with tagged Total-Seq B antibodies (Biolegend) to CD150 ...
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bioRxiv - Immunology 2022Quote: The untouched resting B cells were isolated from the human tonsils by depletion of other cells using MojoSort Human B Cell (CD43-) Isolation Kit (BioLegend), whereas the naïve B cells were isolated using the EasySep Human Naïve B cell isolation kit (Stem Cell Technologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... Intracellular staining for epitopes was achieved using the fixation buffer and intracellular permeabilization buffer kit (BioLegend, San Diego, CA, USA). Antibodies are listed in Table S4 ...
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bioRxiv - Immunology 2022Quote: ... positive selection of the neutrophils was performed by staining the flow through cells with anti-Ly-6G-APC for 20 minutes at 40C followed by isolating the stained cells with anti-APC nanobeads using a mojosort APC nanobead kit (Biolegend). Purity and viability of the cells were checked by flow cytometry using an anti-mouse Gr1 antibody and fixable viability dye ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cytokines in frozen cell culture supernatant or frozen plasma were analyzed with a customized multiplex human or mouse cytokine Legendplex kit (BioLegend). Samples were measured at the MACS Quant Analyzer10 (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... The PBMCs were fixed and permeabilized using Foxp3 / Transcription factor kit (eBioscience) and stained intracellularly with anti-Granzyme-B-Alexa-Fluor-700 (Biolegend), anti-TNF-α-efluor-450 (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: CD14+ monocytes were isolated from PMBCs of healthy human blood using the MojosortTM Human CD14 Selection Kit (BioLegend, Cat# 480026) To generate monocyte derived dendritic cells (moDCs) ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T-cells were enriched from the spleen of OT-II mice using the MojoSort mouse CD4+ T-cell isolation kit (BioLegend) and labelled with CellTrace Violet (5 μM ...
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bioRxiv - Microbiology 2024Quote: Levels of cytokines and soluble mediators present in serum of infected mice were quantified using LEGENDplex Mouse Inflammatory Panel kit (BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Naive CD8+ T cells were isolated by negative selection using the MojoSort Mouse CD8+ Naïve T cell Isolation Kit as per manufacturer’s instructions (BioLegend, >90% purity). Live cells were counted by trypan blue dye exclusion and resuspended at a concentration of 106 cells/mL in complete T cell media (RPMI-1640 (Cytiva ...
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bioRxiv - Immunology 2024Quote: To differentiate between live and dead cells were firstly stained with the Zombie NIR™ Fixable Viability Kit (BioLegend, 423106), the Zombie Yellow™ Fixable Viability Kit (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... plasma and tumor lysates were performed by flow cytometry using a multiplex bead-based system (LEGENDPlex Mouse Th Cytokine Panel and Mouse Inflammation Panel kits, Biolegend) that allowed the simultaneous determination of the concentration of several cytokines ...
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bioRxiv - Cancer Biology 2023Quote: ... were performed as we described previously48 and according to the Mission Bio protocol using the custom-designed 37-gene myeloid panel kit and 48 oligo-conjugated antibodies against all major BM cell types (Biolegend). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Comprehensive surface immunophenotyping was performed using LEGENDScreen Human PE Kit containing 371 pre-titrated antibodies and 354 unique specificities (BioLegend). The experimental run was multiplexed using GFP+ NCI-H358 cells resistant to sotorasib combined with non-fluorescent ...
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bioRxiv - Immunology 2023Quote: CD4+ and CD8+ T cells were isolated from the spleen lymphocytes of healthy mice using MojoSort mouse CD4 nanobeads and a MojoSort mouse CD8α selection kit (BioLegend), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: NK cells were isolated from frozen PBMCs by negative selection using the MojoSort™ Human NK Cell Isolation Kit (BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... were isolated from single cell suspension of lymph nodes and spleen by negative selection using the MojoSort Mouse CD8 T cell isolation kit (BioLegend). Alternatively ...
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bioRxiv - Immunology 2023Quote: Cytokines secreted in the culture supernatant of cytokine-stimulated sip-T were measured by a LEGENDplex Human CD8/NK Panel kit (BioLegend) following manufacturer’s protocol ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... CD3+ T cells were purified from C57BL/6 spleen cells with MojoSort mouse CD3 T cell isolation kit (Biolegend, USA), while CD8+T cells were isolated by adding biotin-labeled anti-CD4 antibody into MojoSort mouse CD3 T cell isolation kit ...
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bioRxiv - Cancer Biology 2022Quote: ... the percentage of apoptotic cells was measured using Annexin V-fluorescein isothiocyanate (FITC)/PI kit using FACSCalibur™ Flow Cytometer (Becton Dickinson Biosciences) in accordance with the manufacturer’s instructions (Biolegend).
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bioRxiv - Cancer Biology 2023Quote: ... FITC Annexin V/Propidium Iodide (PI) staining was performed by FITC Annexin V Apoptosis Detection Kit with PI (BioLegend, 640914). Flow cytometry was performed on BD LSR Fortessa (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... CD45.1+ GFP+ OT-II CD4+ T cells were purified from mouse spleens using magnetic beads (CD4 T cell MojoSort isolation kit, Biolegend) and activated with 10μg/ml recombinant mouse IL-2 IS (Miltenyi ...
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bioRxiv - Bioengineering 2023Quote: ... Mo were then isolated from the suspension of differentiated BMC using a MojoSort™ Mouse Monocyte Isolation Kit (BioLegend #480154). The expression of Mo surface markers was measured by flow cytometric analysis using fluorescently labeled antibodies against CD11b (BioLegend #101207) ...
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bioRxiv - Cancer Biology 2023Quote: Early and late apoptosis in AMO1 shIRE1 cl.1 and shXBP1 cl.1 cells previously treated with Dox was determined with the FITC Annexin V apoptosis detection kit with propidium iodide (PI) (BioLegend). Briefly ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were enriched from the spleen of OT- I mice using the MojosortTM Mouse CD8+ T cell isolation kit (Biolegend) and stained with 5µM CellTrace Violet (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... neutrophils were isolated by negative sorting by magnetic selection using the Mojosort neutrophil isolation kit according to the suggested protocol (Cat# 480058-BioLegend). Purity was checked by flow cytometry using CD11b and Ly6G surface staining ...
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bioRxiv - Cancer Biology 2023Quote: ... Viability of BMNC (0.25 x106) was assessed by Annexin V and 7-AAD staining (APC Annexin V Apoptosis Detection Kit 7-AAD, Biolegend) using FCM.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed twice using ice-cold PBS and stained using Zombie Aqua™ Fixable Viability Kit (Cat. 423101, Biolegend). Cells were washed and resuspended in FACS buffer (1% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were isolated from the spleen and lymph nodes of FVB/N mice using the MojoSort Mouse CD8 T cell isolation kit (BioLegend) as per the manufacturer’s protocol for negative selection strategy ...
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bioRxiv - Microbiology 2024Quote: Cytokine concentrations in supernatants from cultured BMDMΦ or sera of animals were assessed using the Legendplex mouse inflammation kit (BioLegend) following the manufacturer’s instructions as described previously (18 ...
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bioRxiv - Immunology 2020Quote: ... Blood CD4+ T cells were isolated from healthy donor or patient PBMCs using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation ...