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Citations for BioLegend :
6101 - 6150 of 10000+ citations for Mouse Anti Human Papilloma virus type 16 716 325 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: To assess cell viability human neutrophils at indicated times were washed with PBS and incubated with Zombie Aqua Fixable Viability Kit (BioLegend) for 15 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... About 0.3 million cells in 37.5 μl CSM were loaded in each well of a 96-well plate of the Human Cell Surface Marker Screening (phycoerythrin [PE]) Kit (Biolegend). Each well contained 12.5 μl of diluted PE-conjugated antibody in CSM ...
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bioRxiv - Immunology 2020Quote: ... The supernatants were collected and the amount of IL10 in the supernatants were measured using the Human IL10 ELISA MAX (Biolegend) kit according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD4 or CD8 T cell magnetic bead negative selection using the MojoSort Human CD4 or CD8 Selection Kit (BioLegend). Isolated CD4 or CD8 T cells were labeled with CellTrace Violet (CTV ...
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bioRxiv - Microbiology 2021Quote: ... Resting CD4+ T cells were isolated from total PBMCs by negative selection using the MojoSort Human CD4+ T Cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 million cells were resuspended in 1 mL of FACS buffer and 50 μL of Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) was added and left to bind the cells on ice for 15 minutes ...
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bioRxiv - Immunology 2024Quote: Supernatant cytokines were quantified flow cytometrically using the LEGENDplex™ Human CD8/NK Panel as per the manufacturer’s instructions (BioLegend). Data was acquired on LSRFortessa and data analysis was performed with BioLegend LEGENDplex™ Data Analysis Software Suites (BioLegend Qognit Cloud Platform).
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bioRxiv - Microbiology 2024Quote: ... and where indicated containing 25 ng/mL human granulocyte-macrophage colony-stimulating factor (GM-CSF) ((# 572903, Biolegend, San Diego, CA) at 37 °C in a humidified chamber with 5% CO2 in 96-well ...
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bioRxiv - Biochemistry 2024Quote: ... following an overnight incubation at 4°C with the primary antibody against residues 103-108 of human α-synuclein (BioLegend, 807801 [4B12] 1:1000 ...
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bioRxiv - Immunology 2024Quote: ... anti-SHIP-1 (P1C1-A5, PE), human IgE (MHE-18, Alexa Fluor 647), HLA-DR (L243, APC-Cy7) (all from Biolegend); and phospho-p65 NF-κB (K10-895.12.50 ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were used to stain human pDCs either from PBMCs or after purification and culture as described in the text: CD3 (UCHT1, BioLegend), CD14 (HCD14 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in FACS buffer (PBS plus 2% FBS) and blocked with monoclonal antibody to CD16/32 (Human TruStain FcX, #422302, Biolegend) for 10 min at 4°C before staining with surface antibodies ...
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bioRxiv - Physiology 2023Quote: ... zoledronic acid (Novartis Pharmaceuticals) and/or 50 μg/kg (i.p.) recombinant human granulocyte-colony stimulating factor (G-CSF; carrier-free, BioLegend #578604) for three days ...
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bioRxiv - Cell Biology 2023Quote: ... washed with PBS 1% FBS and incubated 10 mins at RT with Human TruStain FcX and True-Stain Monocyte blocker (Biolegend). Antibody mix containing CD14-APCFire750 ...
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bioRxiv - Cancer Biology 2023Quote: ... Comprehensive surface immunophenotyping was performed using LEGENDScreen Human PE Kit containing 371 pre-titrated antibodies and 354 unique specificities (BioLegend). The experimental run was multiplexed using GFP+ NCI-H358 cells resistant to sotorasib combined with non-fluorescent ...
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bioRxiv - Bioengineering 2023Quote: IFNψ was measured from the supernatants of the co-culture experiments (TCE / CAR) using the colorimetric ELISA MAX Standard Set Human IFNψ kit (BioLegend) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: NK cells were isolated from frozen PBMCs by negative selection using the MojoSort™ Human NK Cell Isolation Kit (BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatants were collected from effector-target cell co-cultures and analyzed for IFNy using either ELISA MAX Deluxe Human IFNγ kit (BioLegend) or Human IFNγ DuoSet ELISA (R&D Systems ...
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bioRxiv - Immunology 2023Quote: Supernatants from stimulated T cells were harvested on day 2 and day 6 depending on peak of cytokine production and analyzed using the LEGENDplexTM human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Genomics 2023Quote: ... Cells were pre-incubated with 5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution, BioLegend Cat. No. 422301) per 100µl of cell suspension for 5–10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: Cytokines secreted in the culture supernatant of cytokine-stimulated sip-T were measured by a LEGENDplex Human CD8/NK Panel kit (BioLegend) following manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed in FACS stain buffer and resuspended in diluted Human TruStain FcX Fc blocking reagent according to manufacturer’s instructions (Biolegend, USA) at 4□°C for 10 minutes protected from light ...
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bioRxiv - Immunology 2023Quote: ... Samples were then split into 7 FACS tubes and incubated with 100uL of diluted Human TruStain FcX Fc blocking reagent according to manufacturer’s instructions (Biolegend, USA) at 4□°C for 10 minutes protected from light ...
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bioRxiv - Immunology 2023Quote: ... After blocking of free capture sites on the biosensors with an isotype control (10 µg/mL, human IgG1κ, clone QA16A12, BioLegend) for 600 s ...
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bioRxiv - Genomics 2023Quote: ... the cells were resuspended in 100 μl chilled DPBS with 0.2% BSA and 10 μl of human TrueStain FcX (BioLegend, 422302) and incubated on ice for 10 min to reduce non-specific labeling ...
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bioRxiv - Immunology 2023Quote: ... cells were first resuspended in cell staining media (CSM) (Fluidigm, South San Francisco, CA, USA) with 5ml Human TruStain FcXTM block (Biolegend) for 5 min at room temperature to block Fc receptors ...
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bioRxiv - Immunology 2023Quote: ... were resuspended in labeling buffer (1% BSA in PBS) and underwent Fc blocking for 10 minutes on ice using Human TruStain FcX (BioLegend). Next ...
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bioRxiv - Microbiology 2023Quote: ... 100,000 cells were plated in 96-well round bottom plate and blocked with 1:1000 Human TruStain FcX™ (Biolegend) diluted in washing buffer for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 000-200 000 cells per sample were processed by blocking with 5 µl of Human TruStain FcX Blocking Solution (BioLegend) for 10 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... 200 μl for 96-well plates) supplemented with 1 μg/ml of recombinant human B cell-activating factor (BAFF, BioLegend). 1 µg/ml rHA from H1N1 (PR8 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed once with PBS and incubated in 50 µL PBS containing human Fc block (Supplementary Table 1) and Zombie Aqua viability dye (1:200, BioLegend) for 15 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... iMACs were then washed with PBS plus 2% FBS and incubated for 15 min at RT with 50 ul/mL Human TruStain FcX Blocking solution (BioLegend) in PBS + 2% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... IL-8 concentration in medium samples was measured by using the ELISA MAX Deluxe Set Human IL-8 (BioLegend, 431504) according to the user manual.
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bioRxiv - Cell Biology 2024Quote: ... FBM and ABM cells were resuspended in ice-cold PBS with 5ul of Fc receptor blocker (Human TruStain FcX, Biolegend) and were incubated for 10 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318; anti-CD31 APC/Fire 750, Biolegend 102433; anti-CD45 APC-Cy7, Biolegend 103116 ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318; anti-CD31 APC/Fire 750, Biolegend 102433 ...
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bioRxiv - Cancer Biology 2022Quote: ... and anti-CD20-APC for cells not incubated with protein or with anti-CD20-APC and anti-ROR1-PE (BioLegend) for samples treated with protein ...
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bioRxiv - Immunology 2022Quote: ... and then stained with the appropriate extracellular antibodies (anti-CD45, clone H130, Biolegend; anti-CD3, clone HIT3a, Biolegend; anti-CD4, clone RPA-T4, Biolegend; anti-CD45RA, clone HI100, Biolegend; anti-CD45RO ...
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bioRxiv - Immunology 2022Quote: ... and then stained with the appropriate extracellular antibodies (anti-CD45, clone H130, Biolegend; anti-CD3, clone HIT3a, Biolegend; anti-CD4, clone RPA-T4, Biolegend; anti-CD45RA ...
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bioRxiv - Molecular Biology 2022Quote: ... as well as antibodies to cell surface markers (anti-45 AF700 [Biolegend, 147716], anti-CD3e FITC [Biolegend, 100306], anti-CD4 BV786 [Biolegend, 100552] ...
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bioRxiv - Genomics 2022Quote: ... eF450 (anti-GL7, eBioscience, 48-5902-82), APC (anti-CD86, eBioscience, 17-0862-82) and PE (anti-CXCR4, BioLegend, 146505). Also ...
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bioRxiv - Immunology 2023Quote: ... Panels 2 and 3 incorporated anti-Digoxygenin (RD System, #611621), anti-FITC (Standard BioTools, #3174006B) and anti-Biotin (Biolegend, #409002) metal-tagged antibodies to detect the RNAscope staining output.
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 106 cells were incubated with PE anti-CD31 ([WM59] 303105), PE anti-CD45 ([2D1] 368510) and PE anti-CD326 (EpCAM, [9C4] 324206) antibodies (1/100, BioLegend) and human TruStain FcXTM (1/200 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stimulated with anti-CD3/CD28 (3.5 µg/ml anti-CD3 mAb, BioLegend, #100339; 1 µg/ml anti-CD28 mAb, BioLegend, #102115) in the presence of 5 μg/mL Brefeldin A (BFA ...
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bioRxiv - Immunology 2021Quote: ... anti-TCR-gamma-delta (GL3) and anti-XCR1 (ZET) were from BioLegend. SARS-CoV-2 S(539-546 ...
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bioRxiv - Immunology 2021Quote: Anti-CD3 (clone 2c11) and anti-CD28 (clone 37.51) were from BioLegend. Goat anti-Armenian Hamster antibody was from Jackson ImmunoResearch.
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bioRxiv - Immunology 2022Quote: ... anti-mIFNγ and anti-mIL-12 at 10 μg/mL (from Biolegend except rhIL- 2 ...
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bioRxiv - Immunology 2022Quote: ... Abs were from the following sources: anti-CD45 and anti-CD11b (BioLegend), anti-CD4 and anti-F4/80 (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... anti-dsRNA (J2, 10010, Scicons) or anti-EpCAM-AF488 (9C4, 324210, BioLegend), for 1 hour in PBS with 1% BSA media at room temperature ...
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bioRxiv - Immunology 2019Quote: ... stained on ice with anti-CD25(PE) and anti-CD69(FITC) (Biolegend) for 45-60 minutes ...