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Citations for BioLegend :
6001 - 6050 of 10000+ citations for Goat anti mouse HRP secondary antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 50 µl of flow buffer (1x PBS, 2% FBS, 10mM EDTA, 25mM HEPES) containing anti-CD16/CD32 antibody for Fc receptor blockade (Biolegend, 101320, 1:200) and incubated on ice for 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Positive clones were isolated by limiting dilution and integration of the U1 construct was confirmed with immunofluorescence microscopy and western blotting using an anti-HA antibody (Biolegend cat. no. 901501). Parasite lysate was not boiled prior to western blotting ...
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bioRxiv - Immunology 2024Quote: ... and activated for 72h with 10 µg/mL of plate-bound Ultra-LEAF™ purified anti-human CD3 monoclonal antibody (clone OKT3, BioLegend, cat. 317325) and 5 µg/mL of soluble Ultra-LEAF™ purified anti-human CD28 monoclonal antibody (clone 28.2 ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were labeled for one hour with KRH buffer containing 2% FBE and the following antibodies: anti-HA (BioLegend, #901501, RRID: AB_2565006), APC-conjugated anti-LAMP1 (BioLegend ...
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bioRxiv - Systems Biology 2024Quote: Mice were injected retro-orbitally (under isoflurane anesthesia 2 minutes before euthanasia) with 2 μg of phycoerythrin (PE)–conjugated anti-CD45 antibody (Biolegend, clone 30-F11) to label intravascular leukocytes ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were washed twice with PBS and then incubated with fluorophore-conjugated antibodies (CD81 antibody BioLegend 349501 or Cd44 antibody Biolegend 103023) for 1 hour followed by a PBS wash ...
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bioRxiv - Bioengineering 2024Quote: ... Next, FLAG-tag antibody (Bio-Techne, DYKDDDDK Epitope Tag Alexa Fluor® 488-conjugated Antibody, IC8529G) or HA-tag antibody (BioLegend, Alexa Fluor® 488 anti-HA.11 Epitope Tag Antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... Organoids were cultivated in growth medium as previously described (61) with minor changes (50% mouse Wnt3a-conditioned medium, 50ng/mL mouse Wnt3a recombinant protein (Biolegend, USA), 15% mouse R-spondin1-conditioned medium ...
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bioRxiv - Immunology 2020Quote: ... once a week every week with IL-15 Receptor alpha Fc Chimera (R&D; 2.5μg/mouse) and recombinant human IL-15 (Biolegend, 5 μg/mouse). For immune blockade of PD-1 ...
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bioRxiv - Immunology 2024Quote: The concentrations of various cytokines and chemokines in lung homogenates were determined by LEGENDplex™ (Mouse Inflammation panel and Mouse Proinflammatory Chemokine Panel, BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... Antibodies were as follow: TUJ1 (Tubb3/βIII tubulin) antibody (BioLegend catalog #802001, RRID:AB_291637), FABP7 (Thermo Fisher Scientific Cat #PA5-24949 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were: Rabbit monoclonal antibody specific for Aβ40 (1:2,000, BioLegend 867802) and rabbit monoclonal antibody specific for Aβ42 (1:2,000 ...
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bioRxiv - Systems Biology 2024Quote: ... followed by antibody labelling [1:200 diluted rat Mki67-FITC antibody (Biolegend, 151211). For FB staining ...
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bioRxiv - Cancer Biology 2022Quote: ... for one hour at room temperature followed by APC-Streptavidin secondary (1:500, Biolegend, 405207) and nuclear DAPI (0.1 μg/mL ...
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bioRxiv - Immunology 2021Quote: ... anti-anti-Ly6G (BioLegend; clone 1A8), anti-MERTK (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... anti-CD48 and anti-CD150 (BioLegend) for Lin-Sca-1+c-kit+ (LSK ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated with primary antibody that recognizes an epitope within the humanized Aβ sequence using Purified Anti-B-Amyloid (BioLegend, Cat#803012, Lot#B251533) directly conjugated with horseradish peroxide (HRP ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated for 1 hour at 37 °C with different primary antibodies: anti-β-III-tubulin (1:4000, 801202, Biolegend, San Diego, CA), anti-neurofilament SMI312 (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... Staining for human immunoglobulins was performed in 100 μL with 1:50 (v/v) of the detection antibodies: anti-human IgM Brilliant Violet 650 (clone: MHM-88, Biolegend® Cat. No. 314526), anti-human IgG PE/ Dazzle™ 594(clone ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer and stained on ice for 30 minutes with an Alexa647-labelled anti-human IgG-Fc antibody (Biolegend, San Diego, USA, #409320). Alexa647 mean fluorescence intensities were determined for GFP-positive HEK-293T cells with a FACS Attune Nxt and analyzed with the software Flow Logic™ (IInivai Technologies Mentone ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in PBS complemented with 2% fetal bovine serum and stained with FITC anti-human CD45 antibody (BioLegend #304006CD45, 1:200 dilution) and PE anti-human EPCAM antibody (Miltenyi Biotech #130-113-264 ...
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bioRxiv - Immunology 2022Quote: ... Transfectants were stained for viability using a Live dead blue dye followed by anti-HLA DR DP DQ antibodies (BioRad clone Bu26, Cat. #MCA2497F; Beckman clone I3, Cat. #CO6604366; BioLegend, clone Tü39, Cat. #361704) or anti-HLA DP (Leinco ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.0 × 106 cells were incubated at room temperature for 20 min with 5 µL of APC-labeled anti-CD45 antibody (Clone: 2D1, BioLegend, San Diego, CA, USA). The cell lines were washed in PBS containing 2% FBS ...
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bioRxiv - Immunology 2023Quote: ... Sections were then stained with primary antibody diluted in 1% BSA in PBS for 1 hour with rat anti CD68 (BioLegend 137002 1:400 dilution), rabbit anti Lyve1 (abcam ab14917 1:200 dilution) ...
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bioRxiv - Bioengineering 2024Quote: ... The sections were subsequently stained with a rat anti-human YAP antibody conjugated with PE (dilution: 1:500; clone W19260A; Biolegend, San Diego, CA, USA) for 2 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using the following antibodies: Brilliant Violet 711™ anti-human CD4 (344648, BioLegend; 1 μL/well), APC anti-human CD3 (300412 ...
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bioRxiv - Immunology 2023Quote: ... TCR-transduced CD8+ T cells were rapidly expanded for 5 days using irradiated feeder PBMCs and medium containing 60U/ml IL-2 (Proleukin, Novartis) and Ultra-LEAF™ Purified anti-human CD3 Antibody (clone OKT3, BioLegend 317326, 1:2000). After 5 days ...
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bioRxiv - Microbiology 2023Quote: ... washed once with PBS and stained for 30 min at RT in the dark with anti-CD4 antibody (PerCP-Cy5.5, Biolegend #317428, 1:50 in PBS) and eBioscience Fixable viability dye 780 (ThermoFisher Scientific #65-0865-14 ...
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bioRxiv - Cancer Biology 2023Quote: ... or the following antibody panel where the 488 and 555 channel markers were swapped: Alexa Fluor® 488 anti-Nestin (1:150, Biolegend, #656812, clone 10C2), Alexa Fluor® 555 anti-S100 beta (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained overnight at 4°C in blocking solution with DAPI and the following antibodies and dilutions: Alexa Fluor® 488 anti-Vimentin (1:500, Biolegend, #677809, clone O91D3), anti-Ki67 (1:300 ...
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bioRxiv - Cancer Biology 2023Quote: Other conjugated antibodies used in this study include the following: Alexa Fluor® 647 anti-Tubulin Beta 3 (1:1000, Biolegend, #657406, clone AA10), Alexa Fluor® 488 anti-Vimentin (1:500 ...
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bioRxiv - Neuroscience 2023Quote: C57Bl/6J or LysMGFP mice were injected intravenously with 5 µg of AF647 conjugated to anti-Ly6G antibody (127610; Biolegend, San Diego, CA USA) 3 hours before imaging ...
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bioRxiv - Immunology 2022Quote: Antibody staining was done in the dark for 30 minutes at 4°C in 30 μL of FACS buffer with antibodies and anti-CD16/32 (TruStain FcX™ PLUS; BioLegend, #156604; 1:100). Acquisition was done on a BD FACSCanto™ II or BD LSRFortessa (both BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained for one hour using the following antibodies: Allophycocyanin (APC) anti-human CD274 (5 μL/test; BioLegend #329707; BioLegend, San Diego, CA, USA), APC anti-mouse CD274 (5 μL/test ...
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bioRxiv - Biophysics 2023Quote: ... Transduced T cells were positively selected with a PE selection kit (EasySep, ref: 17684) and an anti-HA PE-conjugated antibody (Biolegend, ref: 901518, clone: 16B12) at a 1:50 dilution to enrich transduced T cells ...
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bioRxiv - Immunology 2024Quote: ... for 15min at RT in the dark following which they were stained with BV510 conjugated anti-CD45 antibody (1:100, clone H130, BioLegend, San Diego, CA, USA) and incubated for 30min at 4°C in the dark ...
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bioRxiv - Cancer Biology 2024Quote: 2*105 N87 and N87-R cells were incubated on ice for 30 min with PE anti-human HER2 antibody (Biolegend, clone 24D2, cat#324406), APC anti-human ABCG2 antibody (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... 60 ng of CD95L-IZ-His and CD95L-IZ after His-tag cleavage was loaded first on a 10% SDS-PAGE followed by a western blot probed with anti-His-tag antibody 1:1000 (BioLegend, San Diego, CA, USA). After His-tag cleavage ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed 5 times in 150 µL of PBST and incubated with 100 µL of streptavidin conjugated to horseradish peroxidase (HRP, BioLegend) diluted 1:2000 in PBS for 1 hour at RT ...
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bioRxiv - Physiology 2024Quote: ... FGF23 was measured using the mouse FGF23 (c-term) ELISA (Immutopics) and IL-6 – using mouse interleukin-6 (IL-6) ELISA (BioLegend, CA, US). Serum 1,25-Dihydroxyvitamin D (1,25(OH)2D ...
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bioRxiv - Immunology 2024Quote: The concentrations of various cytokines and chemokines in lung homogenates were determined by LEGENDplexTM (Mouse Inflammation panel and Mouse Proinflammatory Chemokine Panel, BioLegend, Fell, Germany) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... Concentration of TNF in mouse serum samples (Figure 5b) was quantified using LEGENDplex™ Mouse TNF-α Capture Bead A6 (Biolegend, #740066) as part of the Mouse Anti-Virus Response Mix and Match Panel (Biolgend #740625 ...
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bioRxiv - Cell Biology 2020Quote: ... HA.11 Epitope Tag (mouse mAb 16B12; 901502; Biolegend), Alexa fluorophore-conjugated secondary antibodies (Thermo ...
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bioRxiv - Immunology 2022Quote: ... and Mouse IL-6 ELISA MAXTM Deluxe Set (Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... or isotypic controls (mouse IgG2a (Biolegend, Cat. No.361702) and mouse IgG1 (Abcam ...
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bioRxiv - Immunology 2021Quote: ... using both human and mouse Fc blocking reagents (BioLegend). Cellular suspensions were loaded on a Chromium Single-Cell Instrument (10x Genomics ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse CD8a-Super Bright 702 (53-6.7, Biolegend 100748), FC ...
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bioRxiv - Systems Biology 2021Quote: ... both mouse monoclonal isotype control (Biolegend, San Diego, CA) and omitting the primary antibody (negative method control) ...
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bioRxiv - Immunology 2021Quote: ... Pacific Blue mouse IgG2b isotype control (BioLegend, MPC-11). NKp44-Ig (2249-NK) ...
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bioRxiv - Immunology 2021Quote: ... AlexaFluor 647 mouse IgG2b isotype control (BioLegend, MPC-11), Pacific Blue mouse IgG2b isotype control (BioLegend ...