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Citations for BioLegend :
551 - 600 of 712 citations for Octreotide acetate impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were incubated overnight at 4 °C with primary antibody (Biotin anti-β-Amyloid, 1-16 Antibody, clone 6E10 from BioLegend) dissolved in a carrier solution made with 1% HS ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies, anti-PDGFRa (AB Online, # ABIN726620), anti-Kitl (R&D, #AB- 455-NA) and anti-c-Kit (Biolegend, clone 2B8) were then stained at a 1:200 dilution for 72hrs ...
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bioRxiv - Immunology 2022Quote: ... Immunostaining was performed by incubating the sections overnight at 4°C with the SMI-32 primary antibody (Biolegend: Suppl. Table 1) in incubation buffer ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for 5 minutes at 4°C with TruStain FcX™ (anti-mouse CD16/32) Fc block (clone 93; BioLegend). Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... for 10 min at 4°C followed by an incubation with CD45-BV421 (Biolegend 103133, clone 30-F11, 1:200 dilution) and Ter119-BV421 (Biolegend 116233 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue sections were stained with monoclonal mouse anti-βIII-tubulin primary antibody (Biolegend, Cat. 801202; 1:100 overnight at 4 °C) and goat anti-mouse AF647 (abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated with their respective primary antibodies diluted in 5% skim milk in PBST at 4 °C overnight: purified mouse anti-βIII-tubulin (1:1,000) (BioLegend, cat. 801202), monoclonal mouse anti-GAPDH (1:50,000 ...
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bioRxiv - Developmental Biology 2024Quote: ... then incubated for 1 hr at 4°C with the following antibodies: CD26 (DPP4)-fluorescein isothiocyanate (FITC) (Biolegend, 137806; 1:200), anti-mouse ICAM1-phycoerythrin (PE)/Cy7 (Biolegend ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in 3% BSA/ 5% normal goat serum in PBS for 1h and incubated overnight at 4°C with primary antibodies (β-III tubulin/anti-tuj-1, 1/1000, Biolegend n°801202 ...
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bioRxiv - Immunology 2024Quote: ... cells were co-incubated with human CD16-expressing NK cells (NK92humCD16) for 4 hours at 37°C and the 421™ anti-human CD107a (Biolegend) antibody as a degranulation marker ...
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bioRxiv - Cancer Biology 2024Quote: ... Splenocytes from naive or tumor-bearing AST;Cre-ERT2 mice were mixed with 2×106 C57BL/6 splenocytes and incubated in RPMI-10 for 4 hours at 37°C in the presence of brefeldin A (Biolegend 420601) and TAG peptide (SAINNYAQKL [0.5 μM] ...
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bioRxiv - Immunology 2023Quote: ... Antibody staining was performed at a dilution of 1:100 for 25 minutes at 4L°C in the presence of mouse Fc block (TruStain FcX™, anti-mouse CD16/32, BioLegend) in PBS containing 5% FBS ...
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bioRxiv - Biophysics 2023Quote: ... then cells were stained overnight with DAPI and an Alexa Fluor 647-conjugated anti-cytochrome c antibody (Biolegend, clone 6H2.B4). Stained cells were analyzed using an Attune NxT flow cytometer ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-mouse CD45.1 (Biolegend, cat.: 110735), anti-mouse TER-119 (eBiosciences ...
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bioRxiv - Immunology 2023Quote: ... MΦs were generated by incubating bone marrow cells at 37°C with 40 ng/ml M-CSF (Biolegend, San Diego, CA) in complete RPMI 1640 medium (Gibco ...
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bioRxiv - Immunology 2023Quote: ... surface markers were stained with following antibodies at 1:200 dilution at 4°C for 30 min: CD11b- Pacific Blue (BioLegend, 101224), CD45-BV650 (BioLegend ...
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bioRxiv - Microbiology 2023Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides(25) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with an anti-FcψR antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Reaction was stopped by centrifugation at 4°C and externalized LAMP1 (CD107a) was stained using FITC-coupled anti-LAMP1 antibody (clone 1D4B, BioLegend, #121605) for 20 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: ... centrifuged at 300 rpm for 3 mins followed by incubation at 37°C for 2hrs in the presence of BV421 conjugated anti-CD107a mAb (clone H4A3, Biolegend, #328626). Cell mixtures were then washed in PBS and stained with mAbs to CD56 (FITC or BV605 ...
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bioRxiv - Immunology 2023Quote: ... The reaction was stopped by centrifugation at 4°C followed by staining of cell surface LAMP-1 (CD107a) using FITC-coupled anti-LAMP-1 antibody (clone 1D4B, BioLegend, #121605) for 20 minutes at 4°C in the dark ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were at 8 h post-infection and used fresh or stored at -20°C for up to 1 week and TNF was quantified using mouse TNF-α ELISA MAX kit (BioLegend) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 500,000 cells were resuspended in PBS + 1% FBS and spun at 300g for 10 minutes at 4°C and then resuspended in 25 μL staining buffer (BioLegend #420201). 2.5 μL human TruStain FcX (BioLegend #42230 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation for 10 minutes at 20,000 Xg at 4 °C and VASP was subjected to immunoprecipitation using either His6- specific epitope tag affinity matrix (Biolegend #900501) or control antibody matrix (Novagen #69026) ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and preincubated for 15min with anti-CD16/CD32 Fc-block antibody (10μg/ml; BioLegend, San Diego, CA, 1:50) in FACS buffer followed by a 30min incubation with fluorescence dye-conjugated ...
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bioRxiv - Bioengineering 2023Quote: ... staining was performed for 30 minutes at 4 C with both 70 nM AlexaFluor647-conjugated HER2 and 1ng/μL anti-DYKDDDDK (anti-FLAG) Tag Antibody-PE (Biolegend, 637310). The 5A12 library was first incubated with either 0.5 nM VEGF (Acro ...
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions were incubated for 20 min on ice with TotalSeq™-C anti-human Hashtag oligos (HTOs) (1:500, Biolegend, 394661 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) before run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) prior to run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized for intra-cellular staining with a saponin-based buffer (1% saponin, 10% BSA in PBS) and stained with an antibody for Cytochrome C AlexaFluor 647 (Biolegend, 612310) used at 1:2000 dilution and DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized for intra-cellular staining with a saponin-based buffer (1% saponin, 10% BSA in PBS) and stained with an antibody for Cytochrome C AlexaFluor 647 (Biolegend, 612310) used at 1:2000 dilution and DAPI ...
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bioRxiv - Immunology 2024Quote: ... These cells were stained with a cocktail of mAb conjugated with DNA-barcoded oligonucleotide that are specific for immune cell lineages (TotalSeq™-C Human TBNK Cocktail, BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected from the blots using the following primary antibodies diluted in 1x TBST and incubated overnight at + 4 °C: mouse anti-β-amyloid 1:1000 (6E10, #SIG-39320, BioLegend), rabbit anti-sAPPβ 1:100 (#18957 ...
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bioRxiv - Neuroscience 2024Quote: ... after which the sections were incubated with following primary antibodies at 4 °C overnight: rabbit anti-β-amyloid 1:1000 (6E10, #SIG-39320, BioLegend) to detect diffuse β-amyloid ...
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Langerhans cells regulate immunity in adulthood by regulating postnatal dermal lymphatic developmentbioRxiv - Immunology 2024Quote: ... The media was changed with new serum free media and incubated with his tagged recombinant mouse VEGF-C (10 μg/mL) for 30 min before staining in PBS with anti-his PE (1:100, BioLegend, #362603) and cell surface markers.
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bioRxiv - Neuroscience 2024Quote: ... followed by an 18-hour incubation at 4°C with mouse anti-6E10 antibody (1:1000 in 2% NHS, BioLegend #803001). After three PBS washes ...
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bioRxiv - Immunology 2024Quote: ... for 20 minutes at 37°C and resuspended in cRPMI supplemented with 5 ng/mL recombinant mouse IL-2 (Biolegend #575404). 5×105 cells/well plated in a 96-well round-bottom plate ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then washed with PBS and incubated for 30 min at 4 °C with the following antibodies resuspended in FACS buffer (DPBS with 1% FBS): anti-CD11b-FITC (BioLegend, #101205), anti-CD49f-PE (BD Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then washed with PBS and incubated for 30 min at 4 °C with the following antibodies resuspended in FACS buffer: anti-CD11b-FITC (BioLegend, #101205), anti-Gfap-AF647 (BD Bioscience ...
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bioRxiv - Cancer Biology 2024Quote: ... and the cells were subsequently stained overnight with DAPI and an Alexa Fluor 647-conjugated anti-cytochrome c antibody (Biolegend, 612310) at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Approximately 0.05-0.3 x 106 cells per sample were resuspended in R10 media and incubated with the TotalSeq™-C Human Universal Cocktail (Biolegend) for 30 minutes at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... the following fluorophore-conjugated antibodies were used at the indicated dilutions: CD117 (c-Kit) BV785 (clone 2B8, BioLegend; 1:200 dilution), Ly-6A/E (Sca-1 ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 h and probed with primary antibody at 4 °C overnight with 1:500 anti-His-tag antibody (BioLegend, J099B12) and 1:200 anti-CD95L antibody (Abnova ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells underwent another round of washing and were then stained for 30 min at 4°C with several antibodies: anti-CD8-APC (BioLegend, 100712), anti-PD-1-PE-Cy7 (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... or after a 4-day incubation period at 37°C with 5% CO2 (Day 4) following 30 min antibody staining and additional 15 min Annexin V APC binding (Biolegend, 640920). The antibodies used were as follow ...
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bioRxiv - Cell Biology 2024Quote: ... were thawed in a 37°C water bath for 2 mins and resuspended in 10 ml of warm Staining Buffer (SB; Biolegend #420201). Cells were washed twice at 300 g for 10 mins ...
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bioRxiv - Cell Biology 2024Quote: ... were thawed in a 37°C water bath for 2 mins and resuspended in 10 ml of warm staining buffer (SB; Biolegend #420201) or media buffer (Thermo Fisher Scientific DMEM #12634010 or RPMI #61870010 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with 2% FBS PBS before incubating with a panel of previously titrated 14 barcoded oligo-conjugated antibodies (BioLegend TotalSeq-C), including FITC-anti-CD45 ...
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bioRxiv - Systems Biology 2021Quote: Cells were stained overnight at 4°C in blocking solution with the following antibodies: AlexaFluor® 488 anti-Vimentin (1:1000, Biolegend, #677809), AlexaFluor® E-cadherin (1:200 ...