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Citations for BioLegend :
551 - 600 of 5526 citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Cleared lysates were transferred to new tubes and incubated overnight at 4°C with anti-GFP Nanobody Affinity gel (BioLegend). The affinity gel was then pelleted and washed twice using TEN buffer with 0.1% NP-40 and resuspended in equal volumes of NuPage LDS Sample Buffer (Thermo ...
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bioRxiv - Biophysics 2021Quote: ... DC were stained with PE CD45 (30-F11) and APC CD11c (N418) for 25 minutes at 4°C (all Biolegend). Excess antibodies were removed by washing with HBSS + 0.1% BSA ...
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bioRxiv - Immunology 2021Quote: ... Amplified antibody barcodes were recovered from the supernatant and were processed to generate TotalSeq-C libraries as instructed by the manufacturers (10X Genomics and BioLegend, TotalSeq-C with 10x Feature Barcoding Protocol) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies targeting the various surface ligands and receptors as well as the corresponding control include: FITC anti-human HLA-A,B,C (Clone W6/32, Biolegend), FITC anti-human ICAM-1/CD54 (Clone DX2 ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleared lysates were treated with 8 ng/ml of RNase A and 2U/ml Turbo DNase for 5 min at 37° C and immunoprecipitated for two hours using anti-HA magnetic beads (901502, BioLegend). The beads were washed three times with lysis buffer and three times with PNK buffer (50mM Tris-HCl ...
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bioRxiv - Genomics 2022Quote: ... Cells were stained for the respective cell surface protein for 30 min at 4°C and measured by fluorescence-activated cell sorting (Sony SH800) (BioLegend: CD46 clone TRA-2-10 #352405 - 3μl per 1×106 cells ...
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bioRxiv - Immunology 2021Quote: ... cells stained with antibodies were distributed evenly and exposed to biotinylated trimer or peptide probes preincubated with streptavidin (30 minutes at molar ratio of 1:4 at 25°C) conjugated to PE (405203, BioLegend), BV421 (405226 ...
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bioRxiv - Genomics 2020Quote: ... loss of MHC class I expression in B2M knock-outs was assessed by staining with FITC-conjugated anti-HLA-A,B,C (W6/32, Biolegend). To maximize surface expression of IFNγ regulated proteins ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were fixed for 15 min at 37°C after surface marker and viability dye staining using Fixation Buffer (Biolegend), followed by permeabilization for 1h at −20°C with True-Phos™ Perm Buffer (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... 2×106 single splenocytes or BAL cells were incubated for 4 hours at 37° C in the presence of PMA/Ionomycin and Brefeldin A (Cell Activation Cocktail; BioLegend) or GolgiPlug control (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer and fixed for 20 minutes at 4°C in 100 μl of Fixation Buffer (BioLegend).
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 3 hours at 37 °C with complete IMDM medium with 1x Cell Activation Cocktail (with Brefeldin A, Biolegend). After stimulation ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with brefeldin A 2h at 37°C before surface stain with a mix of CD107a (LAMP1) [clone 1D4B (BioLegend)] and CD107b (Mac-3 ...
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bioRxiv - Cell Biology 2020Quote: Jurkat cells expressing both the GalT-GFP-SNAP and the HA-LAT encoding constructs were incubated for 30 min at 4°C on a wheel with 1:100 of mouse anti-HA Ab (901515; Biolegend). The cells were washed with cold PBS and incubated for 45 min at 4°C on a wheel with membrane-impermeable BG–PEG9–NHS29 ...
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bioRxiv - Immunology 2022Quote: ... or DC in vitro culture (5×105) were washed with PBS at 400 g for 5 min at 4°C and blocked with FcX (BioLegend) for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in TBS-T/5% BSA for one hour at RT and incubated overnight at 4 °C with anti-P62 (SQSTM1) (1:500, #814802, BioLegend), anti-CD107b (LAMP-2 ...
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bioRxiv - Cell Biology 2022Quote: ... bone marrow cells were incubated with cell surface markers for one hour at 4 °C and then washed with PBS before being resuspended with Annexin V binding buffer (Biolegend). Cells were then incubated with FITC Annexin V (Biolegend ...
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bioRxiv - Microbiology 2021Quote: ... cell surface markers were detected by a 30 minute-incubation at 4°C with 4 µg/mL Pacific Blue-conjugated mouse anti-CD14 (clone 63D3, Biolegend), a 1:20 dilution of APC-conjugated mouse anti-CD163 (clone REA812 ...
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bioRxiv - Immunology 2020Quote: ... 2 × 105 WT or CD56-KO NK92 and YTS cell lines were added to 12-well flat-bottomed plates that were pre-coated overnight at 4°C with anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Immunology 2020Quote: 96-well flat-bottomed plates were pre-coated overnight at 4°C with 5 µg/ml anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318; anti-CD31 APC/Fire 750, Biolegend 102433 ...
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bioRxiv - Immunology 2019Quote: Macrophages were stained for 40 min at 4°C with fluorophore-conjugated mAb FITC-anti-CD36 (clone 5-271, Biolegend), or PE-anti-CD209 (clone 120507 ...
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bioRxiv - Immunology 2022Quote: ... BMDCs were pre-incubated for 20 minutes at 37 °C with anti-mouse IFNAR1 (clone MAR1-5A3, 10 μg/mL, Biolegend), anti-mouse IL-12p40 or anti-mouse IL-12p70 Abs or an isotype control before stimulation.
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bioRxiv - Immunology 2019Quote: ... and incubated for 1 h at 4°C in the dark: CD3 (anti-CD3-APC-780; clone 145-2C11, Biolegend), CD4 (anti-CD4-PE-Cy7 ...
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bioRxiv - Immunology 2020Quote: ... the biotinylated proteins were incubated for a minimum of 1 h at 4°C with the streptavidin-conjugates AF647 (Biolegend), BV421 (Biolegend ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with either anti-beta amyloid 6E10 (mouse, 1:1,000 dilution of 1mg/mL stock) (Biolegend, USA), anti-tau MC1 (mouse ...
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bioRxiv - Immunology 2021Quote: ... Cells were harvested 72 hours post transduction and HLA-C expression determined by staining with anti-HLA (W6/32) (Biolegend) antibody ...
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bioRxiv - Immunology 2021Quote: ... Cells were then incubated with bead/Ab mixture for 1 hr at 37°C and then stained with PacificBlue-conjugated anti-CD66b (Biolegend) in PBS ...
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bioRxiv - Immunology 2021Quote: Cell samples went through a series of centrifugations (400g, 5 minutes, at 4°C) and were stained with PE anti-CD45 (30-F11, BioLegend) in staining buffer (see flow cytometry section ...
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bioRxiv - Immunology 2022Quote: ... samples were thawed from −80°C and were stained for surface markers: Zombie UV fixable viability dye (Biolegend, Cat#423107), anti-CD3-BUV395 (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured for 4 h at 37°C in B cell medium containing 1x Cell Activation Cocktail (without Brefeldin A) (BioLegend) and 3h with 1x Protein Transporter Inhibitor Cocktail (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... mice were inoculated with CEA10-mRFP FLARE conidia and lung cells were incubated with 0.05 mg/ml of pHrodo-Zymosan at 37 °C for 2 hours and stained with Survival dye (efluor780, eBioScience) and Ly6G (Pb, BioLegend) or Ly6G (Pb ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Cell Biology 2022Quote: ... were performed after confirming full differentiation into mature MCs using flow cytometric analysis of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421-conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647-conjugated ...
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bioRxiv - Cell Biology 2022Quote: ... Full differentiation into mature MCs was confirmed by expression of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421- conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647- conjugated ...
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bioRxiv - Microbiology 2023Quote: ... for 5 minutes at 4°C and then fixed using commercial fixation buffer for 3 minutes (ref no. 420801, BioLegend). For intracellular staining of Granzyme B and Perforin ...
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bioRxiv - Microbiology 2023Quote: ... single cell suspensions are incubated with 1X Brefeldin-A for 4 hours at 37°C to block secretion of cytokines (ref no.420601, BioLegend). For all ...
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bioRxiv - Cell Biology 2023Quote: ... Red blood cells were lysed by incubation with Red Blood Cell Lysis Buffer containing ammonium chloride for 15 minutes at 4°C (BioLegend). CD34+ selection was performed using AutoMACS cell separation technology following incubation with CD34+ selection beads (Miltenyi Biotech ...
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bioRxiv - Microbiology 2023Quote: ... percoll isolated cells were activated for 5 hours at 37 °C using a Cell Activation Cocktail (1:500, Biolegend, USA). The cells were washed with PBS once and stained with surface staining antibody for 40min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... for 30 minutes at 37°C and then incubated with 10 µg/ml of αASC TMS-1 (clone HASC-71, Biolegend) in blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed in cold PBS/1% (w/v) BSA and stained for 1 hour at 4°C with AlexaFluor647-conjugated anti-BST2 (Biolegend) or control isotype (Biolegend) ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed and stained by incubation at 4 °C for 30 min with 40 µl of mAb anti-membrane marker mix containing CD4 APC/Cy7 (Biolegend), CD19 FITC ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfected cells were then stimulated for 24h at 37°C with plate bound 1μg/ml of anti-CD3 (clone UCHT1, BioLegend #300402) and 2μg/ml of anti-CD28 (clone CD28.2 ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at 37°C and 5% CO2 for one hour to settle and then stimulated with 20 ng/mL recombinant IFNg (BioLegend), 100 ng/mL LPS-EB Ultrapure (Invivogen ...
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bioRxiv - Immunology 2023Quote: ... Cells were permeabilized for 20 mins at 4°C and then stained overnight with IFN-γ-APC (clone 4S.B3, Biolegend) antibody at 4°C.
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were then tagged with mouse Total-Seq anti-mouse hashtags C0306 (Brafi: Hashtag 6) and C0307 (Brafi + C-Me: Hashtag 7) (BioLegend) according to manufacturers’ protocols.
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bioRxiv - Cancer Biology 2023Quote: ... The FCM panel used to analyze EMT phenotype of in vitro tumor cells relied on the use of the anti-mouse pancytokeratins (C-11; Biolegend), CD73 (TY/11.8 ...
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bioRxiv - Cancer Biology 2023Quote: ... or cells cultured in vitro were subjected for surface marker staining with the following reagents for 20 min at 4 °C: Brilliant Violet 650TM-conjugated anti-mouse CD3 Ab (clone 17A2; BioLegend), PerCP/Cyanine5.5-conjugated anti-mouse CD4 Ab (clone GK1.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320 ...
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bioRxiv - Immunology 2023Quote: ... and stained for 30 min at 25 °C with BCL6-PE (7D1, 1:50) and Ki-67-PE-Cy7 (Ki-67, 1:400) (both BioLegend). Cells were washed twice with permeabilization/wash buffer and resuspended in P2 for acquisition on an Aurora using SpectroFlo v2.2 (Cytek) ...