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Citations for BioLegend :
551 - 600 of 1558 citations for 2 Chloro 3 6 chlorohexanoyl pyridine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Staining was done with 3-amino-9-ethylcarbazole (AEC) (BioLegend, Amsterdam, The Netherlands) as HRP substrate ...
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bioRxiv - Immunology 2024Quote: ... and 3 IFNγRKO tumours were labelled with TotalSeq(TM)-C0302 (Biolegend, Cat. 155863). Live cells stained with Zombie NIR and CD45 antibody were sorted based on expression of CD45 using a BD FACSAria™ II ...
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bioRxiv - Neuroscience 2023Quote: ... anti-CX-3-C motif chemokine receptor 1 (CX3CR1; clone #2A9-1, Biolegend), anti-Mer tyrosine kinase (MERTK ...
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bioRxiv - Bioengineering 2022Quote: CPCs and MSCs at passage 3 were suspended in cell staining buffer (Biolegend) (106 cells/100μl) ...
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bioRxiv - Immunology 2022Quote: ... and a soluble mAb anti-CD3 (clone OKT-3, 140 ng/mL; BioLegend), were added to the medium at day 0 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Galectin-3 (1:50; R&D systems, AF1197 & 1:100; Biolegend, 125401), anti-Hsp70 (1:300 ...
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bioRxiv - Genetics 2023Quote: ... cells were stained by Anti-TUBB3 (tubline beta 3; Biolegend Inc, Cat# 801202) in a blocking solution overnight at 4° ...
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Most L1CAM is not associated with extracellular vesicles in human biofluids and iPSC–derived neuronsbioRxiv - Neuroscience 2024Quote: ... SCF (50 ng/ml) (R&D) and IL-3 (50 ng/ml) (BioLegend). For neutrophilic differentiation ...
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bioRxiv - Microbiology 2024Quote: ... and then stained with 3 μl of PE-anti human TNFα antibody (BioLegend) in a 100μl of volume for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... MUTZ-3 cells were also stained with anti-CD14-PE-Cy7 (BioLegend, 367112). Ex-vivo cultured Primary AML samples for were stained with anti-CD34-Alexa Fluor® 488 (BioLegend ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were then blocked in blocking buffer (3% normal serum block (Biolegend, 927503), and 0.4% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were harvested 24 hours later and IL-2 concentrations were determined using serially diluted supernatants and the human IL-2 ELISA MAX Deluxe kit (BioLegend) adapted to 384-well plate format using one quarter of the recommended reaction volumes ...
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bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
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bioRxiv - Bioengineering 2021Quote: ... Bcl-2 anti-apoptotic protein expression was measured by intracellular staining of BMMs with PE-Cy7 anti-Bcl-2 antibody (Biolegend). AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in media (RPMI1640 + 2% FBS) with recombinant human Nectin-2 (Biolegend) or isotype control (ENZO ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
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bioRxiv - Immunology 2021Quote: ... containing anti-CD9 (human HI9a, mouse MZ3), anti-CD63 (human H5C6, mouse NVG-2), anti-CD81 antibodies (human 5a6, mouse Eat-2) from Biolegend, and anti-human ACE2 (R&D Biosystems cat no ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Cancer Biology 2022Quote: Single-cell suspensions were washed after collection and stained in ice-cold Ca+2/Mg+2-free PBS with a viability dye (Zombie NIR, BioLegend) for 20 min at 4°C in the dark ...
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bioRxiv - Immunology 2022Quote: IL-2 ELISA was performed using an ELISA MAX™ Deluxe Set Mouse IL-2 as described in manufacturer’s protocol (BioLegend). The O.D ...
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bioRxiv - Immunology 2023Quote: ... we coated 96 well plates with indicated amounts of anti-CD3ε antibody supplemented with soluble anti-CD28 at 2-times the amount of anti-CD3ε and 5U/mL hL IL-2 (Biolegend). For activation ...
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bioRxiv - Immunology 2023Quote: ... biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) from BioLegend. SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed twice in FACS buffer (PBS + 2% FBS + 1mM EDTA) before staining with anti-pERK1/2 (Biolegend 369506 100x ...
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bioRxiv - Cancer Biology 2023Quote: ... Hepatocytes were resuspended in 2 mL FACS buffer (2 mM EDTA, 0.5% BSA in PBS) and stained with Zombie Violet (1:500) (Biolegend #423113), TruStain FcX™ (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2024Quote: ... human PBMCs were preincubated for 30 min with 2 μg/mL anti-human IL-12 (clone 24910; BioTechne) or 2 μg/mL mouse IgG1 (clone MOPC-21; BioLegend). In functional experiments ...
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bioRxiv - Immunology 2024Quote: ... cells were supplemented with 25 µl/mL ImmunoCult Human CD3/CD28 T Cell Activator (StemCell) and 20 ng/mL Recombinant Human IL-2 (rhIL-2; Biolegend), seeded in a F-bottom 96-well plate using 4×105 cells (200 µl ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 300 g for 5 min at 4°C and cultured in flasks at 106 cells/mL in complete RMPI with 20 ng/mL recombinant mouse IL-2 (rmIL-2; Biolegend). To generate talin-deficient T cells ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 3 times for 5 minutes each with 1xPBS + 0.03% Triton before incubating for 2 hours in secondary solution containing 2% NDS + DAPI (1:1000; Biolegend 422801) + secondary antibodies (Alexafluor 647 donkey anti-rabbit ...
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bioRxiv - Immunology 2024Quote: ... splenocytes (2*107/mL in IMDM medium containing 2% FCS) were simultaneously stained with anti-B220-Alexa647 (clone RA3-6B2 Biolegend) and loaded with 5 μM Indo-1 acetoxymethyl (Indo1-AM ...
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bioRxiv - Systems Biology 2021Quote: ... The levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-α were detected using ELISA kits (Biolegend) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... HTO staining was carried out with 0.5μg of β2M and CD298 antibody-conjugated oligonucleotides (Total-seq B Hastags 4, 6 and 8; BioLegend) with ∼500,000-750,000 isolated single cells ...
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bioRxiv - Immunology 2020Quote: ... mouse TNF-α (430904) and IL-6 (431304) enzyme-linked immunosorbent assay (ELISA) kits were purchased from BioLegend; Griess Reagent System (G2930 ...
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bioRxiv - Bioengineering 2021Quote: ... FITC anti-mouse H-2Kb/H-2Db Antibody (Clone 26-8-6) were from Biolegend (San Diego, CA). Primary antibodies of STING/TM173 (D2P2F) ...
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bioRxiv - Immunology 2022Quote: ... and two hashtag antibodies were built by combining the antibodies MHC I-Biotin (Biolegend, clone 28-8-6) and CD45-Biotin (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... CD45-labelled cells were mixed (maximum 6 samples together) and stained with APC-conjugated FCRL5 antibodies (Biolegend #340305)(for panel #2) ...
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bioRxiv - Immunology 2024Quote: ... and IL-6 were measured by LEGENDplex flow-based 13-plex mouse inflammation panel kit from Biolegend (740446) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cell supernatant was collected after 24 h to measure IL-6 and TNF-α levels using ELISA (BioLegend) according to manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Release of IL-6 and IL-8 were measured using commercial ELISA kits (Biolegend, San Diego, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... injections of a TH17 priming cocktail (n=8; 0.5μg rmIL-6 [575704, BioLegend, USA] and 1μg rmTGF-β1 [763104, BioLegend, USA]) or rest (n=8 ...
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bioRxiv - Microbiology 2024Quote: ... IL-6 and TNF-α ELISA kits at proper dilutions according to the manufacturer’s instructions (BioLegend, CA, USA). For the detection of cytokines in serum and peritoneal fluid ...
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bioRxiv - Immunology 2024Quote: ... the Siglec+ population was sorted by staining the cells with anti-Siglec-7 APC (BioLegend, clone 6-434) or anti-Siglec-9 APC (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse CD63-160Gd (clone NVG-2, BioLegend), anti-mouse CD120b-166Er (clone TR75-32.4 ...
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bioRxiv - Immunology 2021Quote: ... Anti-mouse IL-2 (clone JES6-1A12, BioLegend) antibody was used to capture IL-2 from the supernatants ...
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bioRxiv - Immunology 2020Quote: ... A647 anti-Bcl-2 (633510) (all from BioLegend); APC anti-CXCR4 (558644) ...
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bioRxiv - Immunology 2022Quote: ... anti-CD138 (BV711, 281-2, AB_2562571, BioLegend #142519), anti-CD19 (BV785 ...
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bioRxiv - Cancer Biology 2020Quote: ... Notch2 (Biolegend, 130714, HMN-2-35, 1:80), Notch3 (eBioescience ...
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bioRxiv - Bioengineering 2021Quote: ... IL-2 PE-Cy7 (Biolegend clone MQ1-17H12), IFNγ APC (Biolegend clone B27) ...
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bioRxiv - Immunology 2020Quote: ... CD49d (α4-integrin; FITC, Biolegend, Clone: R1-2), CD88 (PE ...
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bioRxiv - Cell Biology 2020Quote: ... TRA-2-49/6E (1:100, 358702, BioLegend), anti-AFP (1:200 ...
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bioRxiv - Immunology 2022Quote: ... and 10 ng/mL IL-2 (589102, BioLegend) in RPMI-1640 complete medium for 24 hours before killing to differentiate into T cells ...