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Citations for BioLegend :
551 - 600 of 8410 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... 2 μL PE/Cyanine7 anti-human IgD antibody (BioLegend, 348210, clone: IA6-2), 0.013 μg biotinylated SARS-CoV-2 BA.5/BF.7 NTD protein (Sino Biological ...
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bioRxiv - Immunology 2024Quote: ... 100 UI/mL of recombinant human IL-2 (rhIL-2; Biolegend, ref 589108) and 50 μM of 2-mercaptoethanol (Merck ...
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bioRxiv - Immunology 2021Quote: ... counted and up to 1×108 cells were labeled with 1.5-2 μM Carboxyfluorescein succinimidyl ester (CFSE; BioLegend, USA) in 1 mL PBS 1X for 20 min according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Splenocytes from TregΔCd226 and TregWT mice were plated in a 96-well plate at a concentration of 1,000,000 cells/mL with plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155 (1 μg/mL; BioLegend) for 15 or 30 minutes at 37ºC ...
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bioRxiv - Immunology 2020Quote: ... and then plated into 12 well plates previously coated for 2 hours RT with 1 μg/ml of huCD28.2 (BioLegend) and 1 μg/ml of human CD3ε (BioLegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... single cell suspensions were resuspended in FACS buffer (PBS, 2%FBS, 0.01%Tween) and stained for viability dye (1/1000, Biolegend) in 100ul PBS for 10min at RT followed by surface staining with antibody specific for CD45 (30-F11 ...
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bioRxiv - Immunology 2021Quote: ... with the caveat that CD8+ enrichment was achieved by incubating 1-2 million PBMCs with CD8 Mojobeads (Biolegend 480108) for 15 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... then after 2 washes cells were incubated with streptavidin (SA) conjugated to Brilliant Violet 650 (BV650, 1:200, BioLegend) in PBS on ice for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked overnight in Odyssey PBS Blocking Buffer (Li-Cor) and stained for 2 hours at RT with Syn1 (1:1,000; Biolegend) or γH2AX (1:1,000 ...
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bioRxiv - Systems Biology 2021Quote: Cells were stained overnight at 4°C in blocking solution with the following antibodies: AlexaFluor® 488 anti-Vimentin (1:1000, Biolegend, #677809), AlexaFluor® E-cadherin (1:200 ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated overnight at 4°C with purified monoclonal mouse anti-human ASC antibodies (TMS-1) (clone HASC-71) (Biolegend, California, USA) at a final concentration of 10 µg/ml diluted in 10% NGS + 0.5% Triton X-100 in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... samples P008-016 and P048-119 (Suppl. Fig. 4D) were labelled with TotalSeq B Hashtag antibodies 1-4 (Biolegend, clone LNH-94) during the staining procedure described above and pooled in groups of four.
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bioRxiv - Immunology 2021Quote: ... Slides were incubated ON at 4°C with mouse anti-human CD66b antibody (BioLegend, 305102, 1:100 for both IHC and IF), anti-human CD8 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... the pellet was resuspended in PBS and incubated for 30 min at 4 °C with rat FITC-conjugated mAb against CD11b (1:200, BioLegend, CA, USA). After incubation ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted down at 1,500 rpm at 4°C for 5 min and cell pellets were resuspended in 100 uL FACS buffer (PBS + 4% FBS +0.5 mM EDTA) containing Fc block (BioLegend, 101302, 1:200). After 15 min incubation at 4°C ...
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bioRxiv - Immunology 2024Quote: ... and then surface labelled for 30 min at 4°C with TotalSeq-C0182 anti-mouse CD3 (clone 17A2, dilution 1:50, Biolegend, cat # 100263) and TotalSeq-C0002 anti-mouse CD8α (clone 53-6.7 ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated with the following antibodies for 30 min at 4°C: CD45.1-PerCP-Cy5.5 (BioLegend, Catalog No. 110728, 1/500), CD45.2-BV650 (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated 72h at 4°C on a rotating wheel with the secondary antibody (1:500, Alexa-Fluor-555, 405324 P4U/BioLegend UK Ltd) diluted in 100% CAS-Block ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in PBS and stained at 4°C with 100 µL 1:500 Zombie Aqua viability dye (BioLegend, Cat# 423102) and 1:200 mouse FC block (TruStain FcX ...
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bioRxiv - Immunology 2023Quote: ... for 10 min at RT then stained for the following cell surface markers for 30 min at 4°C: CD3 (T cell marker, mouse anti-CD3-BV510, BioLegend, 1:50), CD14 (monocyte marker ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using antigen probe (1.5 μL/well) and the following conjugated antibodies: APC anti-human CD3 (300412, BioLegend; 1 μL/well), PerCP/Cyanine 5.5 anti-human CD19 (302230 ...
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bioRxiv - Immunology 2024Quote: ... Cells were harvested and stained for 30min at 4°C with the following antibodies: anti-CD25 BV785 (Biolegend, Cat: 356139, 1:50), anti-CD127 PE (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were incubated with antibody cocktails in blocking buffer overnight at 4°C in a moisture chamber: Amyloid-β (6E10) (1:100; Biolegend, 803001), anti-CHMP2B (1:100 ...
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bioRxiv - Immunology 2024Quote: ... for 40 minutes at 4 °C followed by a secondary staining with anti-mouse IgG (1:200, Poly4053-PE- Cy7, Biolegend, Cat # 405315) or anti-mouse IgM (1:200 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were fixed with 4% PFA in PBS and exposed to 1:1000 anti-HA mouse monoclonal antibody (BioLegend Cat#901514, RRID:AB_2565336) or 1:1000 anti-FLAG mouse monoclonal antibody (M2 ...
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bioRxiv - Immunology 2021Quote: ... in PBS with 2% FCS and 2% EDTA (FACS buffer) with Fc block (Biolegend) for 25 minutes ...
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bioRxiv - Immunology 2023Quote: ... and 2 ng/ml human interleukin (IL)-2 recombinant (BioLegend, San Diego, CA, 589104).
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bioRxiv - Immunology 2023Quote: ... IL-2 concentration in thawed supernatant was measured by ELISA (Biolegend, IL-2 human). For experiments with KIR2DS1 reporter cells ...
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bioRxiv - Immunology 2024Quote: ... Media included recombinant human interleukin-2 (IL-2) at 230IU/mL – 1,025IU/mL (Biolegend), with the lower concentrations used on days 1-2 ...
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bioRxiv - Immunology 2021Quote: ... Anti-IL-6 (MP5-20F3) and anti-TNF-alpha (MP6-XT22) were from BioLegend.
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bioRxiv - Cancer Biology 2022Quote: ... and with or without anti-IL-6 antibody (20 μg/ml, Biolegend Cat#504512). Cells were then harvested from plates ...
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bioRxiv - Cancer Biology 2022Quote: ... ELISA was conducted using the Mouse IL-6 ELISA Max™ Delux kit (Biolegend), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... in the presence or absence of 20ng/ml of recombinant mouse IL-6 (Biolegend) and/or 200ng/ml of nivalenol (Cayman Chemical ...
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bioRxiv - Immunology 2024Quote: IL-6 ELISA was performed according to the manufacturer’s instruction (Biolegend, Cat. No. 431301) with mouse serum diluted 1:50 ...
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bioRxiv - Bioengineering 2023Quote: ... and values were calculated based on the standard curve for IL-6 (BioLegend 431315), IL-1β (BioLegend 432615) ...
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bioRxiv - Immunology 2024Quote: ... (iii) in the presence of 10 ng/ml of anti-IL-6 (Biolegend, U.S.A.), anti-IL-10 (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... The trypsinized macrophages were stained with anti-Siglec-7 APC (BioLegend, clone 6-434) or anti-Siglec-9 APC (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Diamidino-2-Phenylindole (DAPI) (Biolegend) for live/dead exclusion and cell sorting ...
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bioRxiv - Immunology 2021Quote: ... IL-2 (MQ1-17H12, Biolegend), and IFNγ (4S.B3 ...
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bioRxiv - Immunology 2021Quote: ... and 2 μM monensin (BioLegend). For intracellular staining of IgM ...
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bioRxiv - Cancer Biology 2022Quote: ... and monensin (2 μM, Biolegend) in a humidified incubator at 37°C with 5% CO2 ...
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bioRxiv - Bioengineering 2020Quote: ... beta-2-microglobulin (BioLegend #316306) and Fixable Viability Dye eFluor780 or LIVE/DEAD Fixable Aqua Dead Cell Stain (ThermoFisher #65-0865-14 1:500 dilution ...
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bioRxiv - Immunology 2022Quote: ... and 2 nM Monensin (BioLegend) were added to each well and incubated for further 2 h ...
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bioRxiv - Immunology 2020Quote: ... anti-CD138 (281-2, Biolegend), anti-IgD (11-26c.2a ...
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bioRxiv - Genomics 2021Quote: ... IL-2 (Biolegend, Cat #500310), or CTLA-4 (Biolegend ...
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bioRxiv - Genomics 2021Quote: ... IL-2 (Biolegend, Cat #500310), or CTLA-4 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... 2) huCD45 APC Cy7 (BioLegend), huCD3 PerCp-Cy5.5 (BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... CD138 (clone 281-2, Biolegend), B220 (clone RA3-6B2 ...
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bioRxiv - Cancer Biology 2020Quote: ... CD138 (BioLegend; clone: 281-2) and analyzed for germinal center activated B cells (GCB) ...
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bioRxiv - Immunology 2020Quote: ... IL-2 PE (Biolegend 503808), CD4 PE-Cy7 (Biolegend 100528) ...