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Citations for BioLegend :
5901 - 5950 of 6837 citations for TGFB3 Human Mouse Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Concentrations of cytokines and chemokines present in the serum from SARS-CoV-2 infected AGMs were quantified using a multiplex bead-based assay (1:4 dilution)-the LEGENDPlex Non-Human Primate Cytokine/Chemokines 13-plex (BioLegend, San Diego, CA USA). Analytes detected by this panel are the following ...
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bioRxiv - Cancer Biology 2022Quote: ... Human tissues were incubated for 25 min at 4 °C with the following primary antibodies: CD24-PE (BioLegend, clone ML5, Cat, #: 311105, 1:100), CD44-FITC (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Staining for human immunoglobulins was performed in 100 μL with 1:50 (v/v) of the detection antibodies: anti-human IgM Brilliant Violet 650 (clone: MHM-88, Biolegend® Cat. No. 314526), anti-human IgG PE/ Dazzle™ 594(clone ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer and stained on ice for 30 minutes with an Alexa647-labelled anti-human IgG-Fc antibody (Biolegend, San Diego, USA, #409320). Alexa647 mean fluorescence intensities were determined for GFP-positive HEK-293T cells with a FACS Attune Nxt and analyzed with the software Flow Logic™ (IInivai Technologies Mentone ...
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bioRxiv - Genomics 2019Quote: ... The following antibodies were used for these studies: anti-human CD47-APC (1µl/106 cells in 100µl; clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3), anti-human CD47-FITC (5µl/106 cells in 100µl ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in PBS complemented with 2% fetal bovine serum and stained with FITC anti-human CD45 antibody (BioLegend #304006CD45, 1:200 dilution) and PE anti-human EPCAM antibody (Miltenyi Biotech #130-113-264 ...
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bioRxiv - Cancer Biology 2021Quote: ... The samples were blocked with 0.5 % BSA for 10 min on ice and incubated with the primary mAbs biotin-anti-human CD274 (B7-H1, PD-L1) (clone 29E.2A3, BioLegend, San Diego, CA, USA) or biotin anti-mouse CD274 (clone 10F.9G2 ...
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bioRxiv - Immunology 2020Quote: ... Samples were incubated for 30 minutes in complete FACS buffer (PBS supplemented with 2% FBS) and blocked with Human TruStain FcX™ blocking reagent (BioLegend, San Diego, CA) prior to the staining with antibodies or BiTEs.
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bioRxiv - Immunology 2022Quote: Supernatants were assessed for cytokine production using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) in a V-bottom plate per the manufacturer’s instructions (BioLegend, San Diego, CA, USA). Flow cytometry data were acquired on a BD Fortessa analyzer (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... in the presence or absence of rIFNB (500 ng/mL human [Peprotech, Cranbury, NJ, US]; 50 ng/mL murine [BioLegend, San Diego, CA, US]), with or without neutralizing antibodies or EGTA ...
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bioRxiv - Immunology 2024Quote: ... Isolated CD4+ T-cells were either maintained in a naïve state by seeding in complete growth medium alone or underwent TH1-activation in tissue culture flasks pre-coated with anti-human CD3 antibody (10 µg/mL) (BioLegend, San Diego, CA, US) in complete medium supplemented with anti-human CD28 antibody (5 µg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... wherein each well mixed 50 μL of X2 photobleached conjugated magnetic beads with 50 μL solutions at different concentrations of a recombinant human Interleukin-8 (IL-8) protein (#574202, BioLegend, San Diego, CA, USA). The overall well concentration levels were 0.05 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained for one hour using the following antibodies: Allophycocyanin (APC) anti-human CD274 (5 μL/test; BioLegend #329707; BioLegend, San Diego, CA, USA), APC anti-mouse CD274 (5 μL/test ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed 3 x with 1% BSA before incubating for 1 h in anti-human IgG secondary antibody labelled with either APC (BioLegend, #410712, dilution 1:100) or Phycoerythrin (Abcam ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using the following antibodies: Brilliant Violet 711™ anti-human CD4 (344648, BioLegend; 1 μL/well), APC anti-human CD3 (300412 ...
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bioRxiv - Immunology 2023Quote: ... TCR-transduced CD8+ T cells were rapidly expanded for 5 days using irradiated feeder PBMCs and medium containing 60U/ml IL-2 (Proleukin, Novartis) and Ultra-LEAF™ Purified anti-human CD3 Antibody (clone OKT3, BioLegend 317326, 1:2000). After 5 days ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were spun down to remove cell debris and frozen at -80 °C until analysis using the ELISA MAX™ Deluxe Set Human IFN-γ (BioLegend catalog #430104) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... cells were first stained with biotin-conjugated anti-mouse antibodies against CD3 (BioLegend, clone 145-2C11), CD4 (BioLegend ...
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bioRxiv - Immunology 2020Quote: BM neutrophils were isolated using MACS-column purification (Millentyi Biotec) with biotinylated mouse Ly6G antibodies (BioLegend) and streptavidin magnetic beads (Millentyi Biotec ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were incubated for 10 min using unconjugated anti-mouse CD16/32 antibody (Biolegend, C93, #101301) at 4°C prior to labelling with specific fluorochrome-labelled antibodies ...
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bioRxiv - Immunology 2021Quote: ... Cell sorts were performed with MojoSort Mouse Pan B Isolation Kit II (BioLegend, San Diego, CA) for CD19+ cells and with Miltenyi’s magnetic-activated cell sorting (MACS ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used: mouse anti-Aβ 6E10 (1:500; BioLegend #803001 previously #39320), rabbit anti-BiP (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... the tissues were blocked with the TruStain FcX™ PLUS (anti-mouse CD16/32, Biolegend, USA) antibody (1:100 dilution ...
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bioRxiv - Immunology 2021Quote: ... The FITC-conjugated mouse lineage marker cocktail (CD3, Gr-1, CD11b, CD45R, Ter119) was from BioLegend. APC-H7-anti-CD117 (a.k.a. ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-2 was measured in the supernatants by enzyme-linked immunosorbent assay (BioLegend, BD Biosciences).
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bioRxiv - Immunology 2021Quote: For intracellular IgE staining cells were also pre-blocked with anti-mouse IgE (RME-1, BioLegend) in 1:50 final dilution for 30 minutes ...
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bioRxiv - Microbiology 2019Quote: ... colonic lymphocytes were incubated with anti-mouse CD16/CD32 antibodies (93; BioLegend, Inc., San Diego, CA) to block their Fc receptors ...
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bioRxiv - Cancer Biology 2019Quote: ... and 2×106 cells were incubated with mouse anti-HLA class I antibody (1:200, BioLegend) for 30min followed by secondary antibody incubation for 30min ...
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bioRxiv - Neuroscience 2020Quote: ... and blocked with Ultra-Leaf purified anti-mouse CD16/32 (Clone: 93; BioLegend, San Diego, CA) for at least 20 min ...
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bioRxiv - Microbiology 2021Quote: ... Cells were processed for flow cytometry and stained with BV421 anti-mouse CD45 (Biolegend, Cat# 103134), FITC anti-mouse CD71 (Biolegend ...
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bioRxiv - Biochemistry 2020Quote: ... Secondary detection was with HRP-conjugated goat anti-mouse IgG or donkey anti-rabbit IgG (BioLegend, #405306 and #406401 ...
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bioRxiv - Biophysics 2020Quote: ... using mouse purified anti-HA.11 Epitope Tag monoclonal (clone 16B12) (Biolegend 901502; 1:1,000 dilution), mouse anti-β-actin monoclonal (clone AC-74 ...
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bioRxiv - Immunology 2021Quote: ... cells were purified using negative magnetic bead selection (MojoSort Mouse Pan Dendritic Cell Isolation Kit, BioLegend). 2.5×106 BMDCs per well were cultured for 6h in 6-well plates in RPMI complete (R10 ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated with an anti-mouse CD326 (EpCAM) AlexaFluor 647 antibody (1:100, clone G8.8, Biolegend 118201), Ulex-Lectin 488 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used in this study: mouse monoclonal anti-Aβ1-16 (803001; Biolegend), monoclonal mouse anti-UBE3A (sc-166689 ...
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bioRxiv - Cancer Biology 2020Quote: ... EVs were incubated for 30 min at room temperature with PE-conjugated mouse IgG1 isotype (Biolegend), CD63 (Biolegend) ...
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bioRxiv - Cancer Biology 2020Quote: ... The following antibodies were used: APC conjugated anti-mouse H-2Kb bound to SIINFEKL (141605, BioLegend) and mouse IgG1 ...
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bioRxiv - Cancer Biology 2021Quote: ... and CD56 FITC (or NKp46 AlexaFluor 647TM) and NKG2D PE (or mouse IgG1 PE) antibodies (Biolegend), followed by fixation with 1% paraformaldehyde (Polysciences ...
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bioRxiv - Cancer Biology 2022Quote: ... MCT-treated CD11b+ cells were isolated from tumors using MojoSort Mouse CD11b Selection Kit (BioLegend, 480109). For transfecting every million primary CD11b+ cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed and stained with BV711 conjugated anti-mouse CD8a (Biolegend, 1:200, cat# 563046), PE conjugated anti-mouse CD4 (Biolegend ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... cell surfaces were stained for 30 min at 4°C with anti-mouse B220 (Biolegend, APC), GL7 (Biolegend ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Fc receptors were blocked using TruStain FcX PLUS (anti-mouse CD16/32, 1:200 dilution) (BioLegend). In pilot experiments to determine localization of NC in leukocytes (CD45+ ...
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bioRxiv - Developmental Biology 2022Quote: ... Fc-Receptor block treatment was performed using an anti-mouse CD16/32 antibody (BioLegend, Clone: 93) or anti-human Fc receptor block (biolegend ...
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bioRxiv - Microbiology 2022Quote: Serum IFN-γ was assessed using a custom-made mouse panel 13-plex kit (LEGENDplex, BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... IL-1α and IL-6 were quantified with Mouse IFN-γ ELISA MAXTM Deluxe Set (Biolegend), Mouse IL-1α ELISA MAXTM Deluxe Set (Biolegend ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions were blocked with anti-mouse CD16/32 (clone 93, Biolegend, 1:200 dilution) and stained with fluorescently labeled antibodies for 20-40 min ...
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bioRxiv - Immunology 2020Quote: ... Cells were pre-incubated with TruStain Fc Receptor Block (anti-mouse CD16/CD32, clone 93, BioLegend), then labeled with extracellular antibodies including ...
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bioRxiv - Microbiology 2020Quote: ... one million cells were incubated first with purified anti-mouse CD16/32 antibody (Biolegend, Cat# 101320) in FACS buffer (eBiosciences ...
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bioRxiv - Immunology 2020Quote: ... OVA-specific antibody was detected using biotinylated anti-mouse antibodies (anti-IgG1 [RMG1-1] 0.02μg/ml, anti-IgG2b [RMG2b-1] 0.05μg/ml, Biolegend, CA ...
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bioRxiv - Immunology 2020Quote: Flow cytometry analysis was performed with fluorescently-labeled monoclonal antibodies to mouse CD4-APC (GK1.5, BioLegend) or CD8a-PE (53-6.7 ...