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Citations for BioLegend :
5801 - 5850 of 7153 citations for Biotin Z Antibody Internalization Kit rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... the phenotype of live T-/CAR T-lymphocytes in all the experimental conditions was assessed using the following antibodies: BV510-anti CD3 (clone OKT3, BioLegend), APC-CY7-anti CD3 (clone UCHT1 ...
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bioRxiv - Immunology 2022Quote: ... and PE-conjugated anti-mouse CCR5 (a Th1 marker (27)), CCR3 (a Th2 marker (28)), or CCR6 (a Th17 marker (29, 30)) antibodies (BioLegend). For the MHC ...
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bioRxiv - Immunology 2022Quote: ... the processed fecal samples are incubated with autologous diluted serum and stained as previously described with the following fluorophore-conjugated antibodies: goat anti-mouse PE-IgA and PE-Cy7-IgG1 (clone RMG1-1; Biolegend). Flow cytometry analysis on stained bacteria populations was performed on the LSRFortessa and data analysis was performed on FlowJo ver ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were next incubated in 1x BD Perm/Wash buffer containing AF674-conjugated anti-Ki67 antibody (16A8; Biolegend; 1:100) at 4°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 100 μL Binding Buffer containing 100 nM M1 anti-FLAG antibody labeled with Alexa Fluor 488 and Alexa Fluor 647 anti-human IgG Fc (BioLegend) diluted 1:100 (v/v) ...
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bioRxiv - Cancer Biology 2022Quote: ... bone marrow was subjected to RBC lysis as above and incubated with a biotinylated anti CD133 antibody (Clone 315-2C11, Biolegend), followed by Streptavidin MicroBeads (Miltenyi) ...
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bioRxiv - Cell Biology 2022Quote: ... isolated neutrophils were labeled with a panel of primary antibodies (anti-CD3, anti-CD45, anti-CD19, anti-CD14, anti-CD66b, anti-CD15 from Biolegend) for 20 minutes at room temperature and washed twice ...
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bioRxiv - Biophysics 2022Quote: ... were established by transfection of pIRES-HA-CXCR4 and pIRES-HA-CXCR4-W1955.34L and subsequent cell sorting for equivalent surface expression levels using 1:1000 Alexa Fluor 488-labeled anti-HA antibody (clone 16B12, Biolegend). U87 cells were grown in Dulbecco’s modified Eagle medium (Themo Fischer Scientific ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL were then stained with 2.5 μL FITC anti-human CD19 antibody (BioLegend, 392508), 2.5 μL FITC anti-human CD20 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used to identify MKs: 1:100 anti-mouse CD41-FITC+ and anti-mouse CD42d-APC+ (BioLegend); and MKPs ...
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bioRxiv - Immunology 2022Quote: ... Bound virus was detected with rabbit polyclonal VP1 antibody (1:10000) followed by an anti-rabbit secondary (Biolegend, 1:200). Samples were acquired on an LSRFortessa flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
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bioRxiv - Immunology 2022Quote: ... The clone and dilution of antibodies used for flow cytometry were as follows: CD4-Pacific Blue (Biolegend, OKT4, 1:200), CD69-APC (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... The nanoparticles were washed again and finally resuspended in the 100 µL of assay buffer containing fluorescently labeled AlexaFlora568-detection antibody for IFNγ (Biolegend).
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bioRxiv - Pathology 2022Quote: ... Cells were first stained with Zombie Aqua to exclude dead cells from the analyses and subsequently incubated with anti-CD16/32 antibody (BioLegend) to block Fc receptors ...
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bioRxiv - Physiology 2022Quote: ... the cell pellet was resuspended in 100 μl of Alexa Fluor 647-conjugated anti-human/mouse SSEA1 antibody (catalog no. MC-480; BioLegend) diluted 1:100 in PBS + 2.5% FBS and incubated at room temperature for 10 min ...
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bioRxiv - Genomics 2022Quote: ... Congenic (rather than isogenic) pairings were performed to enable verification of blood chimerism by FACS with antibodies specific to CD45.1 (BioLegend 110705) or CD45.2 (BioLegend 109814 ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were surface stained with series of antibodies (anti-human CD158 [KIR2DL1/S1/S3/S5]-FITC [BioLegend, Cat#339504], anti-human CD158b [KIR2DL2/L3, NKAT2]-FITC [BioLegend, Cat#312604] ...
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bioRxiv - Bioengineering 2022Quote: ... PCs and KCs cultured under xeno-free conditions were analyzed for surface and intracellular markers expression by flow cytometry and immunofluorescence microscopy using antibodies against: CD31 (WM59; Biolegend), CD45 (2D1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for staining of human tumor cells included: anti-human EpCAM-FITC (BioLegend 324204, clone 9C4, dilution 1:30), anti-human/mouse CD49f-APC (BioLegend 313616 ...
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bioRxiv - Immunology 2022Quote: ... Surface staining was performed with anti-mouse Fc receptor antibody (clone 2.4G2, UCSF Hybridoma Core) or Human TruStain FcX (BioLegend #422302) in PBS with 2% FCS for 30 min on ice ...
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bioRxiv - Immunology 2022Quote: ... Samples were centrifuged at 400g for 3 minutes at room temperature and resuspended 50μL in PE-Annexin V antibody diluted 1:20 with Annexin V Binding Buffer (BioLegend, 422201), or 50uL Annexin V Binding Buffer only for controls ...
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bioRxiv - Bioengineering 2022Quote: ... at 0.5 μL/well for 5 hours and washed with PBS for detection of intracellular IFNγ using anti-hIFNγ APC antibody (Biolegend). The frequency of IFNγ positive cells was analyzed by flow cytometry (CytoFLEX II ...
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bioRxiv - Bioengineering 2022Quote: ... and then stained with the fluorescently labeled antibody that detects the corresponding cell surface receptor (anti-EGFR-BV421, Biolegend, Cat: 352911; anti-HER2-BV421, Biolegend, Cat ...
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bioRxiv - Biochemistry 2022Quote: ... neutrophils (1×106 cells/mL) were added to each well in the presence of APC conjugated anti-CD11b antibody CBRM1/5 (BioLegend) at 1 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... samples were stained with the following antibodies in 1X eBiosciences permeabilization buffer for 30 minutes at room temperature: T-bet (1:100; clone 4B10; BioLegend); Foxp3 (1:300 ...
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bioRxiv - Neuroscience 2021Quote: The myelin-depleted single-cell suspension obtained above was incubated (15 min on ice) with anti-mouse CD16/CD32 antibody to block Fc receptors and subsequently incubated with anti-CD11b PEcy7 (Biolegend), anti-CD45 APC (Biolegend) ...
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bioRxiv - Immunology 2020Quote: ... at a total concentration of 5×106 cells/mL in RPMI 1640 + 10% FBS + 1% Pen/Strep +50U/mL IL-2 (R10-50) with 1/100 anti-CD107a-PE antibody (Biolegend) and 1/1,000 Monensin GolgiStop™ (BD) ...
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bioRxiv - Immunology 2020Quote: ... and 2 μM EDTA (FACS buffer) and stained with HLA class I tetramers and/or a directly conjugated antibody specific for CCR7 (clone G043H7; BioLegend) for 10 minutes at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.4)-fixed coronal brain sections were blocked with 10% donkey serum (Jackson Immunoresearch)/PBS plus 0.3% Triton X-100 in PBS and incubated with antibodies to p-S129-α-synuclein (1:1000; Biolegend), and TH (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated with 1.5% normal serum followed by incubation with the primary antibody (biotinylated anti-Aβ – 1:100, BioLegend, USA) for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Binding of His-tagged S1-RBD was detected with Alexa Fluor 488 anti-his tag antibody (clone J095G46, Biolegend, UK) 1:5000 in 25 μL PBST + 0.1% BSA per well ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with anti-CoV-2 Spike RBD mAb (SARS2-02) (13) followed by APC-conjugated goat anti-mouse IgG antibody (Cat# 405308, BioLegend). For surface detection ...
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bioRxiv - Molecular Biology 2020Quote: ... MNCs were harvested by ficoll gradient centrifugation and human hematopoietic cells were identified by flow cytometry using the following antibody cocktail: HLA-A/B/C FITC (clone W6/32, Biolegend), mouse CD45.1 PE-CY7 (clone A20 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were activated for 24h using 2 μg/ml of anti-Cd28 and anti-Cd3ε antibodies (102116 and 100340, BioLegend).
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bioRxiv - Immunology 2020Quote: ... Enriched cells were then stained for the indicated cell surface antigens (Supplementary Table 3) in conjunction with TotalSeq-A oligonucleotide-conjugated antibodies (BioLegend) to allow for barcoding and pooling of different TEC subpopulations and subsequently sorted into 4 subpopulations ...
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bioRxiv - Neuroscience 2021Quote: ... and finally incubated overnight at 4°C with primary antibodies against NKCC1 (T4, 1:1000, DSHB) and tubulin (anti-Tuj1, 1:5000, Biolegend) diluted in the blocking buffer with 0.1% tween ...
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bioRxiv - Microbiology 2020Quote: ... After incubation the cells were washed twice in FACS buffer (PBS, 0.5% BSA, 2 mM EDTA) and stained with a CD19-APC antibody (BioLegend # 302212) to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318 ...
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bioRxiv - Microbiology 2021Quote: ... and cells were stained for 30 min at 4°C with a combination of the following anti mouse immunophenotyping antibodies: from Biolegend: PacificBlue-conjugated anti-Ly-6C (clone HK1.4 ...
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bioRxiv - Cancer Biology 2019Quote: ... Antibodies used to evaluate hypoxic status and surface marker expression were AlexaFluor 647 conjugated anti-HIF-1α (359706, Biolegend, CA), APC-Cy7 conjugated anti-CD8 (344714 ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 2 was used to identify DE cells and contained 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 1 was used to identify CD3+CD14+ cells and contained 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Cancer Biology 2021Quote: ... The following fluorophore-conjugated primary antibodies were used in flow cytometry studies: Alexa Fluor® 488 antimouse CD45 (BioLegend #103122), Alexa Fluor® 594 anti-mouse CD3 (BioLegend #100240) ...
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bioRxiv - Microbiology 2021Quote: T cells were washed and incubated with antibodies (described above) at 4°C for surface staining and cell viability dye Zombie UV (Biolegend). Primary antibodies were detected with anti-human IgG secondary antibody ...
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bioRxiv - Immunology 2021Quote: IFNAR1 on WT and Mt3-/- BMDMϕ was neutralized using 10 μg/ml monoclonal anti-IFNAR1 antibody (BioLegend; Clone: MAR1-5A3) 1h prior and 24h after iLPS (10 μg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were sorted by fluorescent assisted cell sorting on the SH800S cell sorter (Sony Biotechnology) using the following antibodies: FITC anti-human lineage cocktail (Cat# 343604, BioLegend) and APC anti-human CD34 (Cat# 343509 ...
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bioRxiv - Bioengineering 2020Quote: ... The following antibodies and dyes were used: anti-human HLA-A,B,C Pacific Blue (clone W6/32, BioLegend®), anti-HA Alexa Fluor 647 (clone HA.11 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mg of protein (1/10 of input transferred in a new tube) was incubated together with 1 μL of anti-HA antibodies (BioLegend 901502 anti-HA.11 clone 16B12 ...
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bioRxiv - Developmental Biology 2022Quote: ... MGs were localized using the mT/mG reporter or stained using a rabbit anti-K14 polyclonal antibody (1:500, 905301, BioLegend). Nuclei were counterstained with DAPI ...