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Citations for BioLegend :
5751 - 5800 of 7605 citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then stained with CD45-AF657 (BioLegend clone 30-F11, 1:20), CD31-AF647 (BioLegend clone 390 ...
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bioRxiv - Molecular Biology 2024Quote: ... then stained with Zombie UV (BioLegend #423107; diluted 1:1000 in FACS buffer) to exclude dead cells ...
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bioRxiv - Immunology 2024Quote: ... followed by a PE-conjugated anti-mouse IgG1 secondary antibody (RMG1-1, Biolegend). To detect binding of C1s to the cell surface following addition of the C1 complex ...
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bioRxiv - Immunology 2024Quote: ... Brilliant Violet 785™ anti-mouse CD4 antibody (1:50, Biolegend, Clone GK1.5), APC anti-mouse CD8a Recombinant antibody (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... After samples and antibody (anti-α-synuclein, clone 4B12, BioLegend, #807801, 1:50) had been pipetted into the pre-filled assay plate from the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Most antibodies were used at 1:100 dilution for flow cytometry from Biolegend, unless otherwise specified ...
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bioRxiv - Pathology 2024Quote: ... PE anti– mouse Gr-1 (Ly-6G/Ly-6C) (clone RB6-8C5, BioLegend) and Dylight649 anti-GPIb-beta (GPIbβ ...
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bioRxiv - Immunology 2024Quote: ... Cells were then stained with PE anti-mouse IFNγ (Biolegend, XMG1.2, 1:100), APC anti-mouse TNFα (BD Bioscience ...
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bioRxiv - Immunology 2024Quote: ... and anti-CD4 (AF647, Clone RPA-T4, BioLegend, Cat# 300520, diluted 1:25) antibodies were added for incubation overnight in the 4°C fridge ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies and dilutions used were: anti-PAX6 (rabbit, Biolegend, #901301, 1:200), anti-PAX7 (mouse ...
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bioRxiv - Immunology 2024Quote: Antibodies for phenotyping in Panel 1: CD45-Pacific Blue (Biolegend, HI30, Cat. 304029), CD3-PerCP-Cy5.5 (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... AK4-Alexa Fluor® 647 and PAC-1-Alexa Fluor® 647 (BioLegend), AK2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... followed by incubation with 1 μl of TruStain FcX™ PLUS (156603, BioLegend) for 15 minutes at 4°C to block non-specific Fc-mediated interactions ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were stained for surface markers and then prepared for intracellular staining using True-Nuclear Transcription Factor staining kits (Biolegend). For experiments demonstrating stimulation response ...
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bioRxiv - Cancer Biology 2021Quote: NK cells were isolated from C57BL/6 mice (male and female) with the MojoSort mouse NK cell isolation kit (BioLegend) (purity >85 % based on the flow cytometric analysis with NK1.1 and CD49b antibodies) ...
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bioRxiv - Immunology 2021Quote: ... and CD4+ CD25- and CD8+ T cells were purified using the MojoSort Mouse CD4 (supplemented with biotinylated anti-CD25) and CD8 T Cell Isolation Kits (BioLegend), respectively ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were harvested 24 hours later and IL-2 concentrations were determined using serially diluted supernatants and the human IL-2 ELISA MAX Deluxe kit (BioLegend) adapted to 384-well plate format using one quarter of the recommended reaction volumes ...
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bioRxiv - Genetics 2021Quote: ... we negatively isolated memory T cells with a modified Pan T cell magnetic-activated cell sorting (MACSR) kit with anti-CD45RA biotin and followed an optimized version of the CITE-seq protocol with TotalSeqTM-A (BioLegend) oligonucleotide-labeled antibodies for a panel of 31 surface proteins (43) ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining for Foxp3 was performed on single cell suspensions from spleen and LNs specifically using a FOXP3 Fix/Perm Kit (BioLegend).
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bioRxiv - Microbiology 2022Quote: IL-1 cytokine concentrations in the sera of mice or supernatants from cultured BMDMΦ were assessed using the Legendplex mouse inflammation kit (BioLegend) following the manufacturer’s instructions as described previously (42).
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bioRxiv - Molecular Biology 2020Quote: ... CD3 T cells were separated from freshly PBMCs by using a MojoSort™ Human CD3+ T cell Isolation Kit (Biolegend, https://www.biolegend.com/protocols/mojosort-isolation-kits-protocol-1/4599/).
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bioRxiv - Immunology 2021Quote: ... and donor-derived CD4 T cells were analyzed by flow cytometry following magnetic bead–enrichment with MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend).
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bioRxiv - Immunology 2021Quote: ... CD4 T cells from the spleen and peripheral lymph nodes of C57BL/6 mice on 22-24 dpi with LCMV-c13 were harvested and enriched for CD4 T cells using a MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend) prior to surface staining ...
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bioRxiv - Immunology 2019Quote: ... This pooled sample of ∼300 million cells was then evenly distributed across each of the 372 wells of a LEGENDScreen kit (BioLegend) containing reconstituted PE antibodies (Supplementary Table 2) ...
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bioRxiv - Immunology 2019Quote: ... The presence of human GM-CSF in the supernatant of eMSCs co-cultures with CD34+ HSPCs was assessed by enzyme-linked immunosorbent assay (ELISA) using the human GM-CSF ELISA MAX kit (Biolegend) as per manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...
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bioRxiv - Immunology 2021Quote: Paired maternal and infant plasma were thawed and tested in the 13-plex LegendPlex Human Anti-Virus Response Panel kit (Biolegend), to quantify levels of IL-1β ...
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T lymphocytes were purified (> 98%) by negative selection with MojoSort™ Human CD3+ T cell Isolation Kit (Biolegend, USA). Cells were seeded in CD3-coated plates (clone OKT3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed twice using ice-cold PBS and stained using Zombie Aqua(tm) Fixable Viability Kit (Cat. 423101, Biolegend). Cells were washed and resuspended in FACS buffer (1% BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... FOXP3 APC (Clone FJK-16s eBioscience) used after cell permeabilization with the kit Perm Buffer (10X) and Fix/Perm Buffer (4X) (BioLegend), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were blocked/permeabilized using 10% normal goat serum in 0.3% Triton-X in PBS for 3 hours at room temperature followed by additional avidin biotin blocking using a kit according to manufacturer’s instructions (BioLegend, 927301). Primary immuno-staining was performed using antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... All other antibodies used for immune fluorescence and flow cytometric analysis and human IL-1beta ELISA kit were acquired from Biolegend. Human was obtained from In vitro experiments were conducted using complete culture media consisting of RPMI 1640 medium supplemented with 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: To monitor the cytotoxicity of MbxA the release of cytosolic LDH was measured as an indicator of membrane damage using the LDH Cytox Assay Kit (Biolegend).
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were enriched from mouse spleen and lymph nodes by negative selection using MojoSort Mouse CD4 Naive T Cell Isolation Kit (MojoSort, Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and analyzed for apoptosis/necrosis using the APC Annexin V Apoptosis Detection Kit with Propidium Iodide (BioLegend, San Diego, CA). Cells were analyzed using a BD Symphony Flow Cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Cell-free BAL fluid was stored at 4°C and processed within 24hr to measure cytokine/chemokine content using a LEGENDplex bead array kit (BioLegend).
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bioRxiv - Immunology 2020Quote: ... The cells were stained extracellularly to detect ILC surface markers and intracellularly for IFNγ expression using the intranuclear staining kit (Biolegend). Sorted ILC1-like and NK cells were stimulated with human (h ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: Levels of IFN-γ in supernatants from T cell clone recognition assays were measured by ELISA using the ELISA MAX Deluxe Set Human IFN-g kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were isolated using the Mojosort Mouse CD4 Naïve T Cell Isolation Kit (Biolegend, San Diego, CA) using LS columns on a QuadroMACS magnet (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... TCR stimulation was evaluated by measuring IL-2 concentration in culture supernatant using an IL2 ELISA kit (#423001, #423501, #431001 and #421101, BioLegend).
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bioRxiv - Immunology 2022Quote: ... 5×104 Tconv (CD4+CD25-) cells from TFAM control were labeled with CFSE Cell Division Tracker Kit (Cat# 423801, BioLegend) and cultured with mitomycin-treated wild type splenocytes ...
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bioRxiv - Neuroscience 2022Quote: ... CD4+ T cells were isolated from the spleens and draining lymph nodes of 2D2 mice using the CD4+ isolation kit (BioLegend) and co-cultured with irradiated wild-type splenocytes at a ratio of 1:5 ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were analyzed by using either the Legendplex Multi-Analyte Flow Assay Kit for human CD8/NK cells or the human Th cytokine panel (Biolegend).
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bioRxiv - Microbiology 2020Quote: To assess cell viability human neutrophils at indicated times were washed with PBS and incubated with Zombie Aqua Fixable Viability Kit (BioLegend) for 15 min at RT ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed with PBS and stained with Zombie Aqua fixable live-dead kit as per supplier’s instructions (Biolegend, 423101) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The percentage of apoptotic and necrotic cells were then determined by flow cytometry using the FITC Annexin V apoptosis detection kit with PI (BioLegend) following manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... and either directly fixed for ChIP or stained for dead cells using the Zombi NIR Fixable Viability Kit (Biolegend: #423106) as per the manufacturer’s instructions for subsequent FACS sorting (MHV-68-H2BYFP) ...
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bioRxiv - Cancer Biology 2020Quote: ... washed two times with ice cold PBS and processed for staining using FITC Annexin V Apoptosis Detection Kit with 7AAD (Cat # 640922, BioLegend), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD4 or CD8 T cell magnetic bead negative selection using the MojoSort Human CD4 or CD8 Selection Kit (BioLegend). Isolated CD4 or CD8 T cells were labeled with CellTrace Violet (CTV ...