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Citations for BioLegend :
5701 - 5750 of 5857 citations for Mouse Cytochrome C Oxidase Subunit II COX2 MT CO2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... cells were subjected to a live/dead staining with the Zombie NIR™ Fixable Viability Kit (423105; BioLegend) and incubated in the dark for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% BSA and 0.07% NaN3 in PBS) with additional Zombie Aqua™ (1:1,000 Fixable Viability Kit, BioLegend) to stain for vital cells ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after 24h and extracellular IL-2 was measured using the Biolegend ELISAMax Deluxe Kit (Biolegend), according to manufacturer instructions.
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bioRxiv - Genomics 2023Quote: ... CD4+ T-cells were then isolated using the human CD4+ T-cell MojoSort negative magnetic selection kit (Biolegend). Enriched CD4+ T-cells were then rested overnight in a 37°C 5% CO2 incubator in complete RPMI (RPMI1640 ...
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bioRxiv - Immunology 2023Quote: ... both human and murine single cell suspensions were first stained with a viability marker (Fixable Viability Kit, Biolegend) according to manufacturer’s instructions to exclude dead cells ...
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bioRxiv - Neuroscience 2023Quote: ... Cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kit (BioLegend, San Diego, CA) for 20 min at room temperature in PBS combined with FcR blocking antibody (1 μg ...
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bioRxiv - Molecular Biology 2023Quote: Cells were trypsinized and stained with Annexin V and 7-AAD using APC Annexin V staining kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Naïve B cells were isolated from spleens by immunomagnetic depletion using MojoSort Pan B cell Isolation Kit (Biolegend) and cultured in RPMI supplemented with L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 100 μL Zombie NIR (1:1000 in PBS, Zombie NIR™ Fixable Viability Kit, Biolegend, #423106), transferred into a 96-well plate and incubated for 20 min at 4 °C in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were subjected to staining using the Annexin V/Propidium Iodide (PI) apoptosis assay kit (BioLegend, USA) and subsequently analyzed using a BD FACSCalibur flow cytometer (USA) ...
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bioRxiv - Physiology 2024Quote: ... using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-amino-actinomycin D (7-AAD) (BioLegend, #640926), according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... cells were washed once more with PBS and stained with the Zombie VioletTM Fixable Viability Kit (Biolegend, USA) for 15 min following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... and cell pellets were washed with PBS before being treated with the Zombie NIRTM Fixable Viability Kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with PBS and stained for dead cells using Zombie UV Fixable Viability Kit (Biolegend, 423108) or Zombie NIR Fixable Viability Kit (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were stained with the Zombie NIR™ Fixable Viability KIT (780nm of excitation; Biolegend, #423106) at a 1/250 dilution in PBS (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: Detection of apoptotic cells was done by using FITC-Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend#640922). Briefly ...
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bioRxiv - Systems Biology 2019Quote: ... and IL-6 secreted protein level in cell supernatant from 1×106 human MoDM’s was performed by Legend Plex multi-analyte assay kit (BioLegend) according to manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: ... for RNA library construction while modifications for CITE-seq ADT and cell hashing HTO library preparation were performed according to the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 single index protocol (BioLegend). The modifications include the addition of ADT and HTO additive primers to a final concentration of 0.2 uM in the cDNA amplification reaction (step 2.2) ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed with 1xPBS and fixed using buffer of the True-Nuclear Transcription Factor Staining kit (Biolegend). After fixing ...
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bioRxiv - Immunology 2022Quote: ... Brefeldin A solution (420601) and CFSE Cell Division Tracker Kit (423801) were purchased from Biolegend (San Diego, CA, USA). PE anti-mouse IL-4 (12-7041-81) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Both FLT3L and GM-CSF BMDCs were stained with 1:1000 Zombie Green Fixable Viability Kit (BioLegend, Cat# 423111) and were blocked with anti-mouse CD16/32 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... The percentage of apoptotic cells were then determined using the FITC Annexin V apoptosis detection kit with PI (BioLegend) and flow cytometry as per manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from naïve gender- and age-matched WT and CD6Δd3 splenocytes by negative selection using magnetic beads (Mojo sort kit, BioLegend). Isolated cells were activated with 3 μg plate-bound anti-mouse CD3 (145-2C11 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were prepared for flow cytometry two days after media change using the FITC Annexin V kit (Biolegend, 640906). Quantification was performed using a BD FACSCanto 10 flow cytometer.
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bioRxiv - Neuroscience 2020Quote: ... cells were harvested and stained with AV and PI according to manufacturer’s manual (FITC Annexin V Apoptosis Detection Kit with PI; Biolegend). Stained cells were immediately analyzed by flow cytometry (MACSQuant ...
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bioRxiv - Cancer Biology 2021Quote: ... Apoptosis was determined by detecting phosphatidylserine using the APC-Annexin-V Apoptosis Detection Kit with PI (Biolegend, CA, USA). Briefly cells were harvested ...
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bioRxiv - Cancer Biology 2021Quote: Trypsinized and washed Panc02 cells were stained with the FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend) and analyzed using the CytoflexS Flow Cytometer (Beckman Coulter).
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized with a Transcription Factor Staining Kit (eBioscience) and stained using anti-IFNγ (XMG1.2; 200x; BioLegend) and recombinant mouse IL21R Fc Chimera (600x ...
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bioRxiv - Immunology 2021Quote: CD4+ T-cells were isolated from PBMC using the MojoSort™ Human CD4 T Cell Isolation Kit (480009; BioLegend) or by flow-cytometric sorting ...
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bioRxiv - Immunology 2021Quote: For differentiation of live and dead cells we stained cells with the Zombie Violet Fixable Viability Kit according to the manufacturer’s instructions (BioLegend). For surface marker analysis ...
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bioRxiv - Immunology 2022Quote: ... cells were washed again using ice-cold PBS and stained using Zombie Aqua™ Fixable Viability Kit (Biolegend; 423101) for 20 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: Cytokine concentration of the basolateral compartments were measured using the human antivirus response panel (13-plex) kit (LEGENDplex BioLegend). The kit was used according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2022Quote: ... Apoptotic cells were detected using the PE Annexin V Apoptosis Detection Kit with 7-AAD according to the manufacturer’s protocol (BioLegend) and analyzed using an LSRII flow cytometer.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and labeled with propidium iodide (PI) and Annexin-V-FITC (Annexin V FITC Apoptosis Detection Kit with PI, Biolegend) for the detection of necrosis and apoptosis ...
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bioRxiv - Immunology 2024Quote: ... coupled with the protocol TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 (Single Index) from BioLegend.
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were first stained with viability stain (Zombie NIR fixable viability kit, BioLegend, San Diego, California, USA) followed by either BV510-αCD45 (clone ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated from the spleen using Mojosort Magnetic Separation System CD8 Naïve T cell Isolation Kit (BioLegend). Anti-CD3ε Ab (clone 145-2111C ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was harvested and cytokines were quantified using a Legendplex Mix and Match Kit according to manufacturer instructions (Biolegend).
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bioRxiv - Bioengineering 2023Quote: Lung single-cell suspensions were stained for 30 min at RT with Zombie Red™ Fixable Viability Kit (BioLegend) and were blocked with anti-mouse CD16/32 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... tissues were blocked for endogenous avidin and biotin using an avidin biotin blocking kit according to the manufacturer’s instructions (BioLegend). Sections were then incubated with biotin conjugated goat anti-chicken (Vector Labs ...
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bioRxiv - Neuroscience 2023Quote: ... Single cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kit (BioLegend; San Diego, CA) for 20 min at room temperature in phosphate buffer solution pH 7.2 (PBS ...
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bioRxiv - Immunology 2023Quote: Appropriate ELISA kits were used to quantify human or murine IL-6 and/or TNF concentrations according to manufacturer’s recommendations (Biolegend). The 450 nm absorbance was measured by a SpectraMax® plate reader.
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were purified from spleens and lymph nodes using MojoSort™ CD4 isolation kit and magnet (Biolegend). Purities of cells were determined for each experiment using flow cytometry ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in 50 µl of the Annexin V Apoptosis Detection Kit/7-AAD solution (BioLegend) and incubated for 15 min ...
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bioRxiv - Immunology 2024Quote: ... resuspended in 1 ml of PBS containing 1 µl live/dead stain (Zombie UV Fixable Viability kit (Biolegend, # 423107)) and incubated on ice for 30 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were stained for surface markers and then prepared for intracellular staining using True-Nuclear Transcription Factor staining kits (Biolegend). For experiments demonstrating stimulation response ...
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bioRxiv - Genetics 2021Quote: ... we negatively isolated memory T cells with a modified Pan T cell magnetic-activated cell sorting (MACSR) kit with anti-CD45RA biotin and followed an optimized version of the CITE-seq protocol with TotalSeqTM-A (BioLegend) oligonucleotide-labeled antibodies for a panel of 31 surface proteins (43) ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining for Foxp3 was performed on single cell suspensions from spleen and LNs specifically using a FOXP3 Fix/Perm Kit (BioLegend).
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bioRxiv - Molecular Biology 2020Quote: ... CD3 T cells were separated from freshly PBMCs by using a MojoSort™ Human CD3+ T cell Isolation Kit (Biolegend, https://www.biolegend.com/protocols/mojosort-isolation-kits-protocol-1/4599/).
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bioRxiv - Immunology 2019Quote: ... This pooled sample of ∼300 million cells was then evenly distributed across each of the 372 wells of a LEGENDScreen kit (BioLegend) containing reconstituted PE antibodies (Supplementary Table 2) ...