Labshake search
Citations for BioLegend :
5451 - 5500 of 6837 citations for TGFB3 Human Mouse Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... FFPE tissue sections of PDX tumours and their equivalent primary tumour region were subjected to hematoxylin and eosin (H&E) staining or immunohistochemistry using the following antibodies: anti-human CD45 (Clone: HI30; 1:200; BioLegend, #304002), anti-keratin (Clone ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were then stained for transferrin receptor expression by adding 0.25 μg of PE/Cy7 anti-human CD71 [CY1G4] (Biolegend, Cat# 334111) directly to the blocking solution for 30 min at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... Cells were rinsed with FACS buffer (PBS containing 0.5% BSA and 2mM EDTA) for 3 min at 300g and incubated with Fc Receptor Blocking solution (Human TruStain FcX™, Biolegend) for 10 minutes prior to cell surface staining with fluorescently labeled antibodies in Brilliant Stain buffer (BD Biosciences ...
-
bioRxiv - Immunology 2023Quote: Ligand-independent reporter cell activation was assessed by overnight stimulation with 6.25 µg mL-1 Ultra-LEAF™ Purified anti-human CD3 (OKT3, BioLegend, #317326). Cells were incubated in antibody-coated wells and TCR responses were measured by flow cytometric analysis of GFP expression after 16h ...
-
bioRxiv - Cancer Biology 2022Quote: ... LEPs from co-cultures were separated after 10 days for gene expression qPCR analysis by FACS using anti-human CD133-PE (BioLegend, clone7) and anti-human CD271-APC (BioLegend ...
-
bioRxiv - Immunology 2022Quote: ... K562 cells were settled with vehicle-treated or Verbenalin-treated NK cells in the presence of Brilliant Violet 421™ anti-human CD107a (LAMP1) antibody (Biolegend) and GolgiStop™ (BD Biosciences) ...
-
bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...
-
bioRxiv - Immunology 2023Quote: ... for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX, Biolegend, San Diego, California) in PBS with 1% BSA for 10 min at 4C ...
-
bioRxiv - Immunology 2023Quote: ... stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend), and washed once in FACS buffer ...
-
bioRxiv - Microbiology 2023Quote: ... opsonized bacteria were re-suspended in sterile PBS with 2% fetal bovine serum and stained with APC-conjugated anti-human C3b (Biolegend 846106) and FITC-conjugated anti-human C5b-C9 (LSBio C210266 ...
-
bioRxiv - Cell Biology 2023Quote: ... Remaining chemokine levels in the supernatants of cultured cells were measured in triplicate using the commercially available colorimetric LEGEND MAXTM human CCL5 ELISA kit (BioLegend, 440807) according to the manufacturer’s instructions and read with a SpectraMax M5 plate reader (Molecular Devices ...
-
bioRxiv - Immunology 2023Quote: ... buffer (PBS containing 2% FBS and 2 mM EDTA) and then incubated with human Fc receptor block (1:100 dilution, BioLegend, 422302) for 15 minutes on ice ...
-
bioRxiv - Immunology 2023Quote: ... Cells were then stained with the following antibodies on ice for 30 minutes: Alexa Fluor 647 anti-human CD3 (1:100 dilution, BioLegend, 300321), Alexa Fluor 488 anti-human CD4 (1:200 dilution ...
-
bioRxiv - Cell Biology 2023Quote: ... cell suspensions were incubated for 20 min on ice with TotalSeq™-C anti-human Hashtag oligos (HTOs) (1:500, Biolegend, 394661 ...
-
bioRxiv - Immunology 2023Quote: ... 1x106 B cells in 100 μl buffer were incubated with a panel of antibodies including 3 μl FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μl Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
-
bioRxiv - Cancer Biology 2024Quote: Output of CD34+ cells following hematopoietic differentiation of hiPSCs was determined by staining with PE/Dazzle594-labeled anti-human CD34 antibodies (BioLegend, #343534). Prior to addition of antibodies ...
-
bioRxiv - Cancer Biology 2024Quote: ... Stimulated PBMCs were maintained in RPMI-1640 supplemented media with 100 ng/ml CD3 and 10 ng/ml recombinant human IL-2 (Biolegend, 589104).
-
bioRxiv - Bioengineering 2024Quote: ... Supernatants were collected on day 6 and immediately assessed for cytokine and chemokine secretion by LEGENDplex™ using LEGENDplex Human Inflammation Panel 1 (13-plex) with V-bottom Plate (Biolegend catalog #740809 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were washed twice with wash buffer and then incubated with FITC-conjugated anti-HA and PE-conjugated anti-human Fc (BioLegend, #342303) or PE-conjugated anti-Fab (Thermo Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... The secretion levels of IL-1β and TGF-β were quantified using enzyme-linked immunosorbent assay (ELISA) by human IL-1β ELISA Max Deluxe kit (437004, BioLegend) and human TGF-β1 duoset ELISA kit (Dy204-05 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
-
bioRxiv - Immunology 2024Quote: ... These cells were stained with a cocktail of mAb conjugated with DNA-barcoded oligonucleotide that are specific for immune cell lineages (TotalSeq™-C Human TBNK Cocktail, BioLegend) according to the manufacturer’s protocol ...
-
Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... washed cells were resuspended in 100 μL of FACS buffer with 5 μL of PE anti-human TMPRSS2 antibody (BioLegend #378403) per million cells and incubated for 30 min at 4 °C in the dark ...
-
bioRxiv - Bioengineering 2024Quote: ... CD2 target gene activation was quantified by cell surface antibody staining of live cells using APC anti-human CD2 antibody (Biolegend, 309224) followed by flow cytometry on the cell population transfected with both gRNA and dCasMINI plasmids (performed on Cytoflex and analyzed using Flowjo software) ...
-
bioRxiv - Immunology 2024Quote: ... cell pellets resuspended in 100 μl FACS buffer (phosphate buffer saline containing 1% BSA and 0.1% sodium azide) were treated with human TruStain FcX™ (Biolegend #422302) for 15 min on ice followed by incubation with rabbit anti-human LBR antibody (Abcam #32535 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and mouse proinflammatory chemokine panel according to the protocol provided by the manufacturer (Biolegend).
-
bioRxiv - Immunology 2021Quote: ... the cells were collected and stained with APC-Fire750-anti- mouse CD4 antibody (Biolegend), then were detected by flow cytometry ...
-
bioRxiv - Immunology 2021Quote: ... FcR block was performed by incubating cells with purified anti-mouse CD16/32 (BioLegend) for 5 minutes at room temperature followed by surface staining with antibody mixture at 40 in the dark for 20 minutes ...
-
bioRxiv - Immunology 2021Quote: Sorted mMDSCs (50,000) were stimulated with 50 ng/ml recombinant mouse M-CSF (Biolegend) in Iscove’s Modified Dulbecco’s Medium (IMDM ...
-
bioRxiv - Immunology 2020Quote: ... after which Fc receptors were blocked using TruStain FcX (anti-mouse CD16/32, Biolegend). Cell surface staining was performed at room temperature (for details of antibodies and panels refer to Additional File 1 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Mouse anti-Smi32 (non-phosphorylated anti-Neurofilament H (NF-H)) (BioLegend Cat# 801701 RRID:AB_2564642) (1:500) ...
-
bioRxiv - Developmental Biology 2020Quote: ... H5 (mouse anti-P-Ser2 RNA Pol II, Biolegend Cat. # 920201, diluted 1:50), and OIC1D4 (mouse anti-PGL-1 ...
-
bioRxiv - Microbiology 2022Quote: ... Endotoxin-free recombinant mouse IL-1α and IL-1β proteins were purchased from BioLegend.
-
Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... Fc receptors were blocked using TruStain FcX™ (anti-mouse CD16/32) Antibody (Biolegend, Cat# 101320 ...
-
bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal anti-NPC proteins (FXFG repeat-containing nucleoporins) (1:1,000; mAb414; 902907, Biolegend), rabbit monoclonal anti-BANF1/BAF (1:200 ...
-
bioRxiv - Cancer Biology 2019Quote: ... antibodies used for analysis of myeloid cells were: anti-mouse CD45-BV510 (103138; BioLegend), F4/80-BV785 (107645 ...
-
bioRxiv - Molecular Biology 2020Quote: ... SERINC5-iHA was detected with mouse α-HA (clone 16B12, Biolegend, diluted 1:5000), Gag was detected using mouse α-p24 (Millipore ...
-
bioRxiv - Immunology 2020Quote: ... in light-protected conditions: PE-Cy7 conjugated anti-mouse CD11b (clone M1/70, BioLegend, San Diego ...
-
bioRxiv - Immunology 2019Quote: ... and TNF-α in supernatants were measured using a custom mouse LEGENDplex assay (Biolegend) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... The following antibodies were used for western blot: mouse anti-HA (1:2,000, BioLegend), rabbit anti-GFP (1:10,000 ...
-
bioRxiv - Immunology 2019Quote: ... incubated with 10 µg/ml AF647-labeled mouse TCRβ antibody (Biolegend, catalog H57-597) for 30 minutes on ice ...
-
bioRxiv - Immunology 2019Quote: ... The latter was further detected by using DyLight488 anti-mouse IgG (Biolegend, catalog 405310). Molecular densities were calculated assuming the following diameters:13 μm for Raji B cells (Hui et al. ...
-
bioRxiv - Neuroscience 2019Quote: ... The retinal sections were stained with mouse anti-tubulin β3 (Tuj1, 1:500, BioLegend) and rabbit anti-myosin IIB (1:100 ...
-
bioRxiv - Neuroscience 2019Quote: ... The retinas were stained with mouse anti-tubulin β3 antibody (Tuj1, 1:500, BioLegend) following the steps mentioned above (see Immnunohistochemistry of whole-mount retinas) ...
-
bioRxiv - Microbiology 2019Quote: ... GcrA (1:1,000 dilution, rabbit)36and RpoD (1:250 dilution, Mouse IgG2b) (Neoclone, BioLegend) antibodies were used ...
-
bioRxiv - Immunology 2021Quote: ... Cells were then incubated with TruStain FcX anti-mouse CD16/32 Fc block (Biolegend) for 10 min on ice in Cell Stain Buffer (Biolegend) ...
-
bioRxiv - Immunology 2021Quote: ... and then stained with Alexa Flour 647 conjugated rabbit anti-mouse IgG (Biolegend, USA). Cells were extensively washed and mounted with VECTA SHIELD (containing DAPI ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell surface staining was performed with mouse B220-PerCP/Cy5.5 (clone RA3-6B2; BioLegend) or human CD19-PE (clone HIB 19 ...
-
bioRxiv - Bioengineering 2020Quote: ... and LEGENDplex Mouse Th Cytokine Panel were purchased from Biolegend (San Diego, CA, USA). OVA 257-264 (SIINFEKL peptides ...