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Citations for BioLegend :
5451 - 5500 of 7153 citations for Biotin Z Antibody Internalization Kit rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Cell surface integrins were labelled with fluorochrome-conjugated antibodies [Alexa Fluor 488 anti-integrin β1 (Clone: HMβ1-1, Biolegend), APC anti-integrin α1 (Clone ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with primary antibodies diluted in 1% NDS-PBS-Tx (1:1,000 for mouse anti-HA, #901514 BioLegend; 1:1,000 for rabbit anti-TOM20 ...
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bioRxiv - Immunology 2021Quote: ... cells were permeabilized with 0.5% saponin for 15 min and intracellularly stained with PE-anti-perforin (clone B-D48) antibody (Biolegend) for 20 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... cortical cells from embryonic mice were labelled with Brilliant Violet 421™ anti-mouse CD90.2 (Thy1.2) antibody (BioLegend, #140327). Cells were incubated with antibody at 4 °C for 20 minutes followed by washing with wash buffer (Ca2+/Mg2+ free PBS ...
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bioRxiv - Immunology 2021Quote: The following antibody mixture was made and used to stain the flow through and labeled fractions: B220-BV510 (Biolegend) at 1:20 ...
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bioRxiv - Microbiology 2020Quote: ... lymph node and lung cells were stained with antibodies against multiple surface antigens: anti-CD45 (2µg/ml, 30-F11; Biolegend), anti-SiglecF (1µg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated in 5% dried non-fat milk powder dissolved in TBS-Tween 0,1% (TBST) for 1 h at room temperature (RT) and subsequently incubated with primary anti-HA antibody (Biolegend) followed by incubation by secondary goat HRP-conjugated anti-mouse IgG (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were stained with an antibody that recognizes the luminal epitope of the human LAMP1 protein (clone H4A3, BioLegend) conjugated with Alexa fluor 488 or 647 for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... we collected the beads and labeled them with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
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bioRxiv - Genomics 2020Quote: ... Cells were then stained with a panel of 9 further DNA-oligonucleotide conjugated antibodies (0.33 μg/ml anti-CD71: BioLegend 113802 conjugated with ADT9 [CGAAGAAGGAGT], 1 μg/ml anti-CD41: BioLegend 133919 conjugated with ADT3 [TGTCCGGCAATA] ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were blocked in FSC buffer (2% FBS/1x PBS) containing 0.5 µg anti-CD16/32 antibody (TruStain FcX Plus, BioLegend) for 10 min at 4°C to block non-specific binding of antibodies via Fc receptors ...
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bioRxiv - Bioengineering 2022Quote: ... low endotoxin (Super affinity) (Acro) followed by a secondary staining with APC conjugated anti-human IgG Fc Antibody (Biolegend) and RFP expression ...
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bioRxiv - Immunology 2022Quote: ... PVDF membrane transferred proteins were probed with antibodies against anti-Siglec 5/14 (clone 1A5, Biolegend, San Diego, CA).
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bioRxiv - Cancer Biology 2022Quote: ... using Kawamoto tape62 (Section-lab) as described before.63 Cryosections were stained with antibodies against B220 (RA3-6B2, Biolegend), CD2 (14- 0021-85 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... primary antibody 4G8 was incubated overnight at a 1:1000 dilution at room temperature (RT) (Biolegend, San Diego, CA). HRP-linked secondary goat anti-mouse antibody at a 1:1000 dilution (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Cell surface staining was performed for 30 min at 4ºC with fluorochrome-conjugated monoclonal antibodies to mouse antigens purchased from Biolegend: CD45 (30-F11) ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4°C overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution, 846302, Biolegend), rabbit anti-CD31 (1:200 dilution ...
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bioRxiv - Microbiology 2022Quote: The HA epitope was detected with mouse monoclonal antibody (mAb) HA.11 (diluted 1:1000) (BioLegend, item no. 901515) or rabbit polyclonal antibody (pAb ...
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bioRxiv - Immunology 2022Quote: ... The plate was washed five times after 1-hour secondary antibody incubation and developed with tetramethylbenzidine substrate (Biolegend, 421101). The reaction was stopped with 1 M phosphoric acid after 20 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Mononuclear cells were enriched over Ficoll-PaqueTM premium 1.084 (Cytiva, cat #17544602) and stained using the following antibodies (all from Biolegend): Lineage Cocktail-PacBlue ...
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bioRxiv - Bioengineering 2022Quote: ... and then stained with the fluorescently labeled antibody that detects the corresponding cell surface receptor (anti-EGFR-BV421, Biolegend, Cat ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained 20 min with APC-conjugated anti-CD24 antibody (1:100 dilution, 138506, Biolegend, San Diego, CA) and FITC-conjugated anti-Epcam antibody (1:500 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... and harvested bone marrow cells and splenocytes were stained with the following fluorochrome-coupled antibodies: CD3e-Pacific Blue (BioLegend) clone 17A2 ...
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bioRxiv - Immunology 2020Quote: ... These cells were stimulated with 10μg/ml of anti-CD3 (OKT-3) and anti-CD28 (28.2) antibodies (Ultra-LEAF™, Biolegend) in R10-50-15 for 48 hours ...
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bioRxiv - Microbiology 2020Quote: ... the same protocol was followed except that the primary antibody was Anti-Escherichia coli RNA Polymerase α (Biolegend, #663104) used at a 1:10,000 dilution and the secondary antibody was an Anti-Mouse IgG HRP conjugate antibody (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... 600 μL of WCE was incubated overnight at 4°C with 10μg of monoclonal RNA Poll antibody (Biolegend, USA), 2μg of anti-myc antibody (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells overexpressing Rab35 were detected using an anti-HA primary antibody (data not shown; Rabbit, Cell Signaling; Mouse, Biolegend) + Alexa Fluor-405 secondary antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were washed and incubated with fluorescently-labeled antibodies (B220 V500, FAS FITC, CD38 Alexa fluor 700; Biolegend) for 20-40 min ...
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bioRxiv - Immunology 2020Quote: Non-specific binding of antibodies to Fc-receptors was blocked with anti-mouse CD16/CD32 (TruStain fcX from BioLegend) in single cell suspensions prepared from Spl ...
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bioRxiv - Immunology 2020Quote: ... Antigen experienced CAR T cells were stained for CD69 expression using APC-conjugated anti-human CD69 antibody (Biolegend #310910). Flow cytometric data was acquired using a BD LSRFortessa with BD FACSDiva software ...
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bioRxiv - Immunology 2019Quote: ... OVA-specific IgG1 was detected using biotinylated anti-mouse IgG1 monoclonal antibody (0.02µg/mL RMG1-1, Biolegend – CA, USA) followed by SA-HRP (1:2000 dilution #P030701-2 ...
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bioRxiv - Bioengineering 2020Quote: ... dilutions of the serum were mixed with a constant 3 nM of an HRP conjugated anti-OVA antibody (BioLegend) and incubated for 2 h at room temperature ...
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bioRxiv - Pathology 2022Quote: ... or B cell supernatant pre-treated with a recombinant antibody to deplete IL-10 (IgG2b, clone JES5-16E3, Biolegend). As controls ...
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bioRxiv - Genomics 2022Quote: CRISPR/Cas9 RNP-treated Th2 cells were stained intracellularly with APC anti-GATA3 antibody (Biolegend #653806, 1:40 dilution) at room temperature for 30 minutes using True-Nuclear Transcription Factor Buffer Set (Biolegend #424401 ...
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bioRxiv - Immunology 2022Quote: ... blood leukocytes from Ldlr-/- and T-AbcdkoLdlr-/- mice were stained with a cocktail of antibodies against TCRβ-PB (Biolegend), CD8-PE ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then blocked in FSC buffer (2% FBS/1x PBS) containing 0.5 μg anti-CD16/32 antibody (TruStain FcX Plus, BioLegend) for 10 min at 4°C to block non-specific binding of antibodies via Fc receptors ...
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bioRxiv - Biochemistry 2022Quote: ... per reaction were incubated with 200 μl antibody binding buffer [1X PBS/ 0.02% Tween 20] and 5 μg of anti-FMRP (Biolegend) and anti-FXR1(Santa Cruz Biotechnology #sc-374148) ...
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bioRxiv - Immunology 2022Quote: ... 48 h and 72 h and analyzed by flow cytometry after staining with an anti-Notch2-PE antibody (BioLegend). Dead cells were discriminated from living cells using a fixable live/dead cell staining kit (Invitrogen).
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bioRxiv - Neuroscience 2022Quote: ... and then incubated 1:100 with antibodies directed against cell surface proteins CD11b-BV421 (BioLegend, 101251, clone M1/70), CD45-Percp-Cy5.5 (BioLegend ...
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bioRxiv - Cancer Biology 2019Quote: ... Incorporation of BrdU in proliferating intestinal epithelial cells was detected using an anti-BrdU antibody (Biolegend, 1:100 dilution) after deparaffinization of the tissues ...
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bioRxiv - Genomics 2019Quote: ... CD4+ T helper cells were sorted as naive T cells (CD4+CD25−CD45RA+) or memory T cells (CD4+CD25−CD45RA−) using the following antibodies: anti-human CD45RA-PERCPCy5.5 (Clone HI100, Biolegend), anti-human CD4-APC-Cy7 (Clone OKT4 ...
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bioRxiv - Bioengineering 2019Quote: ... and CD31+ cells were isolated using flow cytometry sorting after staining cells with anti-mouse CD31 antibody (Biolegend 102417) and plated onto Vitronectin-coated plates (1mL/well at 1µg/cm2 ...
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bioRxiv - Immunology 2019Quote: ... Vα7.2+ cells were isolated from PBMCs by labelling with anti-Vα7.2-PE antibody (Clone; 3C10, BioLegend, San Diego, USA) followed by separation with anti-PE magnetic beads using MS columns (both from Miltenyi Biotec ...
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bioRxiv - Microbiology 2019Quote: ... the same general protocol was followed except that the primary antibody was Anti-Escherichia coli RNA Polymerase α (Biolegend) used at a 1:10,000 dilution and the secondary antibody was an Anti-Mouse IgG HRP conjugate antibody (Promega ...
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bioRxiv - Immunology 2020Quote: ... The following antibodies were used for flow cytometry and stimulation: FITC-labelled anti-HLA-A2 (BB7.2, Biolegend, 1:40), AlexaFluor® 647-labeled anti-HLA-A,B,C (W6/32 ...
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bioRxiv - Cancer Biology 2021Quote: ... Expression of H-2Kb and PD-L1 were assayed in the CD45− fraction generated from WT and CCN4-KO YUMM1.7 tumors using the antibodies: CD274 (PD-L1)/PE (clone 10F.9G2, BioLegend) and H-2Kb/APC (clone AF6-88.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Fc receptors were blocked by incubating cells with TruStain FcX™ PLUS anti-mouse CD16/32 antibody (Biolegend, USA) for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... 1;100 dilution) and an antibody against SR-B1 for FACS (363208, clone m1B9; dilution 1:100) were from Biolegend. The SARS-2-S mAb (GTX632604 ...
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bioRxiv - Microbiology 2021Quote: ... in PBS containing the primary antibodies at a 1:100 dilution: mouse anti-DC/L-SIGN (Biolegend, cat. 845002), rabbit anti-DC-SIGN (Cell Signaling ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were trypsinized and incubated with antibodies specific for FITC conjugated CD44 (338804) and PerCP conjugated CD24 (311113; Biolegend) or trastuzumab conjugated with Alexafluor 647 according to the manufacturer’s instructions (A20181 ...