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Citations for BioLegend :
501 - 550 of 771 citations for N N Dimethyl 4 4 Trimethylsilyl Ethynyl Phenyl Ethynyl Aniline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Cell-free BAL fluid was stored at 4°C and processed within 24hr to measure cytokine/chemokine content using a LEGENDplex bead array kit (BioLegend).
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bioRxiv - Immunology 2020Quote: ... 2 × 105 WT or CD56-KO NK92 and YTS cell lines were added to 12-well flat-bottomed plates that were pre-coated overnight at 4°C with anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Immunology 2020Quote: 96-well flat-bottomed plates were pre-coated overnight at 4°C with 5 µg/ml anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Neuroscience 2021Quote: ... and finally incubated overnight at 4°C with primary antibodies against NKCC1 (T4, 1:1000, DSHB) and tubulin (anti-Tuj1, 1:5000, Biolegend) diluted in the blocking buffer with 0.1% tween ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318 ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318; anti-CD31 APC/Fire 750, Biolegend 102433 ...
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bioRxiv - Microbiology 2021Quote: ... and cells were stained for 30 min at 4°C with a combination of the following anti mouse immunophenotyping antibodies: from Biolegend: PacificBlue-conjugated anti-Ly-6C (clone HK1.4 ...
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bioRxiv - Immunology 2021Quote: ... Cells from each individual mouse were stained using BioLegend TotalSeq B hashtags (Hashtags 1-4) and an anti-PE oligonucleotide barcode (BioLegend). Cells from each compartment (i.e ...
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bioRxiv - Microbiology 2021Quote: T cells were washed and incubated with antibodies (described above) at 4°C for surface staining and cell viability dye Zombie UV (Biolegend). Primary antibodies were detected with anti-human IgG secondary antibody ...
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bioRxiv - Immunology 2022Quote: ... or polarized to Th1 phenotype by culturing in T cell media additionally supplemented with IL-12 (20 ng/mL) and anti-IL-4 (10 µg/ml) (Biolegend). After 48 h ...
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bioRxiv - Immunology 2022Quote: ... and then cell surface staining was performed for 30 min at 4°C with fluorochrome-conjugated monoclonal antibodies to mouse antigens purchased from Biolegend: XCR1 ...
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bioRxiv - Synthetic Biology 2020Quote: ... the media-trypsin mix was aspirated and the cells were fixed via incubation in 50 µL of 4% formaldehyde (BioLegend) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... the biotinylated proteins were incubated for a minimum of 1 h at 4°C with the streptavidin-conjugates AF647 (Biolegend), BV421 (Biolegend ...
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bioRxiv - Bioengineering 2020Quote: ... and then cells were fixed with 4% PFA at room temperature for 10 minutes and permeabilized with intracellular staining perm wash buffer (BioLegend), and stained with the appropriate intracellular antibodies ...
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bioRxiv - Synthetic Biology 2021Quote: ... The samples were then flash frozen and defrosted for 4 rounds to lyse the cells and used on an IL-10 detection ELISA (Catalog 430601, Biolegend).
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bioRxiv - Bioengineering 2020Quote: ... cells were recovered from wells and were incubated at 4°C for 30 minutes with antiCD3 antibody (Biolegend, CA, USA) and then were examined by flow cytometry.
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bioRxiv - Immunology 2021Quote: ... cells were blocked for 1 hour at 37⁰C with 300µl of Duolink® blocking solution then stained overnight at 4⁰C with purified mouse anti-Phosphoserine (Biolegend) or purified mouse IgG1 isotype control (clone MG1-45 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed with FACS buffer and labeled with PE conjugated mouse anti-human TNFRSF12A antibody (clone ITEM-4, Biolegend) for 30 minutes on ice ...
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bioRxiv - Immunology 2020Quote: ... For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend. All final reactions were developed with TMB substrate (Thermo scientific ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes from uninfected or infected mice were incubated at 37C° in 5% CO2 for 4 h in medium containing Monensin and Brefeldin A solutions (Biolegend). Cells were then stained for surface antigens (TCRβ-PacificBlue and NK1.1-PE) ...
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bioRxiv - Immunology 2021Quote: Cell samples went through a series of centrifugations (400g, 5 minutes, at 4°C) and were stained with PE anti-CD45 (30-F11, BioLegend) in staining buffer (see flow cytometry section ...
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bioRxiv - Immunology 2021Quote: ... isotype controls and inhibitor of VLA-4 were used at 1 μg/ml: LEAF™ purified anti-CD29 (clone HMβ1-1, 102209, BioLegend), LEAF™ purified anti-CD31 (clone MEC13.3 ...
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bioRxiv - Immunology 2020Quote: ... Slides were incubated with monoclonal antibodies overnight at 4°C using anti-CD3 (100202, clone 17A2, BioLegend, San Diego, CA) to detect T-cells ...
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bioRxiv - Neuroscience 2021Quote: ... Except for immunohistochemistry for SOX-9 in which a β-gal staining was performed at 37°C for 3h followed by fixation with PFA 4% for 30 min at RT and incubation with unmasking antigen solution (927901; Biolegend). Sections were blocked and permeabilized in a permeabilization solution for 2h and incubated in goat polyclonal antibodies against SOX-9 (1:40 ...
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bioRxiv - Immunology 2023Quote: ... Murine cells were stained for 30 min at 4°C with the following antibodies (all from Biolegend, unless otherwise stated): CD3 APC(Clone:17A2 Cat:100236 ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were precooled for 20 min at 4 °C and then incubated with HDL-SOG for 40 min at 4 °C with 50 μg HDL per ml PBS or with anti-SCRB1 antibody-SOG (BioLegend, 363201 ...
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bioRxiv - Immunology 2023Quote: ... CAR-T cells were cocultured with NALM6GL cells at indicate E/T ratio for 4 hours with the presence of 1x Brefeldin A (Biolegend). Cells were first stained with the Live/Dead-NearIR dye before fixed ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CXCR1-PerCP (1:50; 8F1/CXCR1, BioLegend), CD11b-PE (1:100 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CXCR1-FITC (1:100; 8F1/CXCR1, BioLegend), CD19-PerCP (1:50 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CD235a-PECy7 (1:20; HI264, BioLegend), CD31-APCCy7 (1:20 ...
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bioRxiv - Cell Biology 2023Quote: ... at the indicated concentrations for 4 hours in 100 µL of RPMI 1640 medium containing 0.2% BSA with Brefeldin A Solution (BioLegend, 420601). After treatment ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4°C with the following antibodies: CD138-FITC (1:20; MI15, BioLegend), CD45-PerCP-Cy5.5 (1:20 ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed in cold PBS/1% (w/v) BSA and stained for 1 hour at 4°C with AlexaFluor647-conjugated anti-BST2 (Biolegend) or control isotype (Biolegend) ...
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bioRxiv - Microbiology 2023Quote: ... for 5 minutes at 4°C and then fixed using commercial fixation buffer for 3 minutes (ref no. 420801, BioLegend). For intracellular staining of Granzyme B and Perforin ...
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bioRxiv - Microbiology 2023Quote: ... single cell suspensions are incubated with 1X Brefeldin-A for 4 hours at 37°C to block secretion of cytokines (ref no.420601, BioLegend). For all ...
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bioRxiv - Immunology 2022Quote: ... Following combinations of cytokines and antibodies were used for the differentiation of T cells: for Th1 cells - 1 µg/ml anti-IL-4 (clone 11B11; Biolegend), 30 U/ml rhIL-2 (NIH) ...
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bioRxiv - Immunology 2023Quote: ... 100 uL/well) were restimulated with coated anti-CD3 antibody (1 ug/mL, 100 uL/well, 4 °C overnight, Clone: OKT3, Biolegend) and soluble anti-CD28 antibody (2 ug/mL ...
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bioRxiv - Immunology 2023Quote: ... the cells were re-suspended in flow cytometry staining buffer (PBS containing 4% FBS and 2 mM EDTA) and blocked with TruStain FcX (mouse or human, Biolegend) for 5 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Red blood cells were lysed by incubation with Red Blood Cell Lysis Buffer containing ammonium chloride for 15 minutes at 4°C (BioLegend). CD34+ selection was performed using AutoMACS cell separation technology following incubation with CD34+ selection beads (Miltenyi Biotech ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with Cell Stimulation Cocktail (plus protein transport inhibitors) (eBioscience) for 4 hrs or Cell Activation Cocktail (Biolegend) in the presence of Brefeldin A and Monensin for 5 hrs before staining.
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bioRxiv - Immunology 2023Quote: ... Cells were permeabilized for 20 mins at 4°C and then stained overnight with IFN-γ-APC (clone 4S.B3, Biolegend) antibody at 4°C.
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bioRxiv - Developmental Biology 2023Quote: ... NPCs were we pelleted and washed once with AutoMACS buffer before resuspending AutoMACS with in DAPI (dead cell dye DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 422801, BioLegend) and DRAQ5 (DRAQ™ Live cell dye ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained for 20-30 min at 4°C with an antibody mix composed of: Zombie UV viability dye (BioLegend), anti-CD45RA (Beckman) ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed and stained by incubation at 4 °C for 30 min with 40 µl of mAb anti-membrane marker mix containing CD4 APC/Cy7 (Biolegend), CD19 FITC ...
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bioRxiv - Biochemistry 2023Quote: ... The NMM/DMSO was incubated for 40 hours at 4°C while the primary antibody anti-tubulin beta- III (#802001, BioLegend), diluted at 1:2000 in 0.15% Triton-X in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated for one hour in PE anti-human CD71 Antibody in the dark at 4°C (1:100; Biolegend). Cells were washed twice with PBS and then and then analysed using a flow cytometer (FACsFortessa ...
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bioRxiv - Neuroscience 2022Quote: ... according to manufactures protocol and blocked in 5% BSA/TBS for 1 h and incubated overnight at 4 °C with the following antibodies: mouse anti-CD9 (1:1000; Biolegend), mouse anti-CD63 (1:1000 ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated rasburicase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were counted and then incubated at 4 °C for 25 min with FITC anti-mouse CD45 (Biolegend, 103107, USA), Brilliant Violet 650 Ly6G (Biolegend ...