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Citations for BioLegend :
501 - 550 of 3979 citations for IL 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and IL-17A (clone TC11-18H10.1; BioLegend). The samples were acquired using the FACSCanto II flow cytometer and analyzed with FlowJo software.
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bioRxiv - Immunology 2023Quote: ... 50 ng/mL IL-27 (577402, BioLegend), 10 ng/mL IFNβ (581302 ...
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bioRxiv - Bioengineering 2023Quote: ... IL-10 (APC/Cy7, Cat#505036, BioLegend), CD45 (BUV395 ...
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bioRxiv - Microbiology 2023Quote: ... IL-2-APC (MQ1-17H12 clone, Biolegend), TNFα-AF700 (Mab11 clone ...
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bioRxiv - Immunology 2023Quote: ... administrations of 0.25 µg IL-33 (BioLegend) or 0.218U papain (Sigma ...
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bioRxiv - Immunology 2023Quote: ... BV421 anti IL-17A (TC11-18H10.1, Biolegend), BV750 anti-IFNγ (XMG1.2 ...
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bioRxiv - Immunology 2023Quote: ... mouse IL-23 (10 ng/ml, Biolegend), human TGFβ-1 (5 ng/ml ...
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bioRxiv - Genetics 2023Quote: ... and Il-7R⍺ (BV605, clone A7R34, BioLegend). The cells were incubated in the antibody solution for 90 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... IL-17 FITC (BioLegend, clone TC11-18H10.1), CD45 BV 605 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... and IL-7 (20 ng/ml, Biolegend) for 2 days ...
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bioRxiv - Immunology 2024Quote: ... CD218α (IL-18r)-PE (BioLegend, Cat#157903), CD62L-BV650 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... 10 ng/mL recombinant IL-33 (Biolegend), 1 µg/ml LPS (Invivogen) ...
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bioRxiv - Immunology 2023Quote: ... and IL-15 [2 ng/ml] (BioLegend), media change 3 times per week and culture splitting 1 time per week ...
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bioRxiv - Immunology 2024Quote: ... IL-2: BV605 (Biolegend, Clone: JES6-5H4), TNFα (Biolegend ...
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bioRxiv - Pathology 2022Quote: ... cell suspensions were centrifuged again at 300 g for 5 min and then preincubated with 2.5 µg/ml Fc blocker (156604 for mouse, 422302 for human, BioLegend) on ice for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognizes residues 1-5 of human α-synuclein (50)] (BioLegend) at 1:4,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognises residues 1-5 of human α-synuclein (7)] (BioLegend) at 1:4,000 ...
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bioRxiv - Developmental Biology 2024Quote: ... 45 µL of cell suspension was transferred to a new 1.5 mL Eppendorf tube (approximately 350,000 cells) and 5 µL of Human TruStain FcX™ Fc Blocking reagent (Biolegend) were added to block unspecific binding of the hashing antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse interleukin (IL)-4 PE (504104) and anti-mouse IL-17 PE (517008) (BioLegend, San Diego, CA, USA) were used ...
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bioRxiv - Immunology 2021Quote: ... spleen T cell IL-1β/IL-23 plus spermidine treatment group (SPT sper) with hashtag 3 (BioLegend, Cat# 155805); lymph node cell IL-1β/IL-23 stimulation group (LN stim ...
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bioRxiv - Pathology 2022Quote: Serum levels of IL-6 and TNFα were measured by a mouse ELISA MAX Standard IL-6 kit (BioLegend) and a mouse ELISA MAX Standard TNFα kit (BioLegend).
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bioRxiv - Immunology 2022Quote: ... BMDMs supernatant and intracellular IL-12p70 were quantified by using ELISA MAXTM Deluxe Set Mouse IL-12 (p70) (Biolegend).
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s instructions and stained with PE anti-IL-6 and PE-Cy7 anti-IL-10 (JES3-9D7) Abs (Biolegend).
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bioRxiv - Neuroscience 2023Quote: ... and stained for intracellular cytokines (IFN-γ PE #505808, IL-4 BV421 #504120, IL-17A PE-Cy7 #506922, Biolegend) with the Cyto-Fast™ Fix/Perm Buffer Set (#426803 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were stained at the same time with the APC conjugated surface marker antibody CD45 after a 5 min blocking step with Human TruStain FcX solution (20x, BioLegend) diluted in 1 % BSA/PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... while the MDR1 replicons were stained with 5 μL of PE anti-human CD243 (ABCB1) antibody clone 4E3.16 (Biolegend, US). After staining for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... Cells were then blocked with human Fc-block (Miltenyi) in the presence or absence of purified anti-CD36 antibody (Clone 5-271; Biolegend) at 10 μg/ml for a further 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... followed by a 30 minute incubation with the TotalSeq-C antibody pool and 5 μl of a PE/Cyanine7 anti-human CD8 antibody (BioLegend) at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 5×104 B cells were first incubated with 5 µg/ml anti-IgM complexes and 1 µg/ml recombinant Human CD40L (Biolegend) for 30 mins and then 1×104 Tfr cells were added ...
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bioRxiv - Genomics 2023Quote: ... Cells were pre-incubated with 5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution, BioLegend Cat. No. 422301) per 100µl of cell suspension for 5–10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 000-200 000 cells per sample were processed by blocking with 5 µl of Human TruStain FcX Blocking Solution (BioLegend) for 10 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells stably expressing EPO-eGFP and A1At-mCherry were incubated with 1ug/mL Brefeldin A (Biolegend) for 12 hr ...
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bioRxiv - Immunology 2022Quote: ... or polarized to Th1 phenotype by culturing in T cell media additionally supplemented with IL-12 (20 ng/mL) and anti-IL-4 (10 µg/ml) (Biolegend). After 48 h ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
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bioRxiv - Molecular Biology 2023Quote: ... Conditioned medium was collected and IL-1β levels were assessed with ELISA MAXTM Deluxe Set Mouse IL-1β (Biolegend, 432604) following manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... with recombinant IL-15/IL-15RA complex (100 ng/ml; Thermo Fischer, Waltham, MA) or recombinant mouse IL-6 (50 ng/ml, BioLegend), TGF- β1 (1ng/mL) ...
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bioRxiv - Physiology 2023Quote: ... IL-6 and IL-10 in the homogenates were measured using LEGENDplexTM Mouse Custom Panel (BioLegend, San Diego, CA, USA). Assay was performed in the Attune NxT flow cytometer (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... BV421 anti-mouse IL-4 (Cat: 504127 Biolegend), APC-Cy7 anti-mouse CD3 (Cat ...
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bioRxiv - Immunology 2021Quote: ... Anti-mouse IL-2 (clone JES6-1A12, BioLegend) antibody was used to capture IL-2 from the supernatants ...
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bioRxiv - Immunology 2022Quote: ... IL-17A (TC11-18H10.1, Biolegend, #506938, 1:100), IL-17F (9D3.1C8 ...
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bioRxiv - Immunology 2022Quote: ... Mouse IL-1α ELISA MAXTM Deluxe Set (Biolegend) and Mouse IL-6 ELISA MAXTM Deluxe Set (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... IL-21 (10 ng/ml, Biolegend, Cat#571204), IFN-γ (20 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... IL-17 (Clone TC11-18H10.1, BioLegend, Cat 506915), Tbet (Clone 4B10 ...
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bioRxiv - Immunology 2019Quote: ... 100U/mL IL-2 (Roche Diagnostics or Biolegend) was used throughout ...
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bioRxiv - Bioengineering 2021Quote: ... IL-2 PE-Cy7 (Biolegend clone MQ1-17H12), IFNγ APC (Biolegend clone B27) ...
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bioRxiv - Microbiology 2020Quote: ... and IL-10 (2µg/ml, JES5-16E3; Biolegend) was performed on ice for 30 minutes.
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bioRxiv - Immunology 2022Quote: ... and 10 ng/mL IL-2 (589102, BioLegend) in RPMI-1640 complete medium for 24 hours before killing to differentiate into T cells ...
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bioRxiv - Microbiology 2022Quote: ... IL-8 was subsequently quantified by ELISA (Biolegend), as reported (Antosca et al. ...
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bioRxiv - Immunology 2022Quote: ... FITC-anti-IL-10 (505006, BioLegend Europe BV), PE-anti-IL-13 (12-7133-81 ...