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Citations for BioLegend :
501 - 550 of 7384 citations for 7 Bromo 2 4 iodo phenyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... the human IL-2 ELISA kit (catalog no. 431801; BioLegend) and human IFN-gamma kit (catalog no ...
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bioRxiv - Immunology 2024Quote: ... Brefeldin A (5 μg/ml) and Monensin (2 μM) (BioLegend) were added to the cultures during the last 4 h of incubation to allow intracellular cytokine accumulation ...
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bioRxiv - Cancer Biology 2024Quote: ... 50,000 Calcein-labeled (2 µM, Biolegend, San Diego, CA, USA) COLO 205 cells were co-cultured with 500,000 human whole leukocytes for 4 hours with or without 5-A-RU (50 nM ...
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bioRxiv - Immunology 2019Quote: ... in the presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). Streptavidin M280 Dynabeads were conjugated to biotinylated HLA-C*05:01 refolded with P2-AV or P2-AW as described below.
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bioRxiv - Immunology 2021Quote: ... counted and up to 1×108 cells were labeled with 1.5-2 μM Carboxyfluorescein succinimidyl ester (CFSE; BioLegend, USA) in 1 mL PBS 1X for 20 min according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Splenocytes from TregΔCd226 and TregWT mice were plated in a 96-well plate at a concentration of 1,000,000 cells/mL with plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155 (1 μg/mL; BioLegend) for 15 or 30 minutes at 37ºC ...
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bioRxiv - Cell Biology 2019Quote: ... with 2□mM EDTA for flow analysis or resuspended in PBS (pH 7.2) with 2□mM EDTA and 1□μg/ml DAPI (Biolegend) for sorting ...
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bioRxiv - Immunology 2020Quote: ... and then plated into 12 well plates previously coated for 2 hours RT with 1 μg/ml of huCD28.2 (BioLegend) and 1 μg/ml of human CD3ε (BioLegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... single cell suspensions were resuspended in FACS buffer (PBS, 2%FBS, 0.01%Tween) and stained for viability dye (1/1000, Biolegend) in 100ul PBS for 10min at RT followed by surface staining with antibody specific for CD45 (30-F11 ...
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bioRxiv - Immunology 2021Quote: ... with the caveat that CD8+ enrichment was achieved by incubating 1-2 million PBMCs with CD8 Mojobeads (Biolegend 480108) for 15 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... then after 2 washes cells were incubated with streptavidin (SA) conjugated to Brilliant Violet 650 (BV650, 1:200, BioLegend) in PBS on ice for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked overnight in Odyssey PBS Blocking Buffer (Li-Cor) and stained for 2 hours at RT with Syn1 (1:1,000; Biolegend) or γH2AX (1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... IL-2 and IL-7 were removed from ILC2 culture and additionally cultured for 4 hr before restimulation with IL-33 (Biolegend, #580508) plus NMU (R&D systems ...
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bioRxiv - Immunology 2019Quote: ... CD169 (PE; 7-239; BioLegend), CD28 (PECF594 ...
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bioRxiv - Immunology 2020Quote: ... Thy1.1 (clone OX-7, BioLegend), CD8 (clone 53-6.7 ...
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bioRxiv - Immunology 2020Quote: ... Thy1.1 (clone OX-7, BioLegend), CD8 (clone 53-6.7 ...
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bioRxiv - Immunology 2021Quote: ... anti-CD169 (Biolegend, 7-239) antibodies and acquired with an Attune NxT Flow Cytometer (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... or 7-aminoactinomycin D (BioLegend) for all other experiments.
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bioRxiv - Immunology 2021Quote: ... anti-CD169 (Biolegend, 7-239) antibodies and acquired with an Attune NxT Flow Cytometer (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... anti-GL7 (GL-7, Biolegend), anti-CD95 (Jo2 ...
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bioRxiv - Immunology 2020Quote: ... CD38 (Biolegend clone HB-7), IFNg (Biolegend clone 4S.B3 ...
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bioRxiv - Immunology 2021Quote: ... Thy1.1 (clone OX-7, BioLegend), CD8α (clone 53-6.7 ...
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bioRxiv - Immunology 2023Quote: ... 7-AAD (BioLegend or BD) was used to discriminate dead CTV+ cells via flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... Viability dye 7-AAD (Biolegend) were added to samples before acquisition on a flow cytometer.
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bioRxiv - Immunology 2023Quote: ... CD38-FITC (HB-7, Biolegend), CD45RA-PerCP-Cy5.5 ...
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bioRxiv - Biophysics 2023Quote: ... Dye 7-AAD (Biolegend, USA) was used for dead cell exclusion from the analysis ...
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bioRxiv - Developmental Biology 2024Quote: ... or 7-aminoactinomycin D (BioLegend). Cells were double sorted for purity.
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bioRxiv - Cell Biology 2024Quote: ... Streptavidin PECy-7 (BioLegend, #405206), Ly6A/E (Sca-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were resuspended in sorting buffer with 7-AAD (BioLegend, 420404; 1:100) to gate the viable cells ...
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bioRxiv - Immunology 2023Quote: ... biotin anti-CD3 (7 μg/mL) Abs for 1 h at room temperature (BioLegend, clones RA3-6B2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Dead cells were excluded using 7-aminoactinomycin D (7-AAD, eBioscience) and zombie violet (BioLegend). FACS analysis was conducted using a MACSQuant analyzer (Miltenyi Biotec) ...
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bioRxiv - Immunology 2019Quote: ... anti-4-1BB PE (4B4-1, BioLegend), anti-RORγt PE (Q21-559 ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-4 (1 ng/mL; BioLegend) was added to primary co-culture for 4 days ...
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bioRxiv - Immunology 2024Quote: ... Anti-4-1BB (clone 4B4-1, Biolegend), anti-CD107a (clone H4A3 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained in FC buffer (2% FCS in PBS) with anti-CD3-APC conjugated antibodies (1:80; BioLegend) and LIVE/DEAD Fixable Near-IR Dead Cell Stain (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... CD18 (clone CBR LFA-1/2, 366310), ITGB7 (clone FIB504, 121006), and CD68 (clone Y1/82A, 333814) were acquired from Biolegend.
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bioRxiv - Cancer Biology 2019Quote: ... or 1 million cells (analysis)No in FACS buffer (PBS with 2% FBS, 1X pen/strep and 1 mM EDTA) containing human TruStain FcX (Biolegend) and mouse TruStain FcX (anti-mouse CD16/32 ...
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bioRxiv - Bioengineering 2022Quote: ... Splenic cells at 1 × 106 were co-cultured with target cells at 1:2 effector to target ratio in the presence of anti-CD107a (Biolegend), brefeldin A and monensin and a serial dilutions (6x ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained at 4°C for 30 min in Hanks Balanced Salt Solution-2% FBS with the following markers: anti-THY1 AF700 1:100 (Biolegend), anti-PDGFRalpha PE 1:100 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... for 30’ at RT in PBS containing 2% FCS and 1 mM EDTA prior to secondary staining with streptavidin-BrilliantViolet 510 (Biolegend) for 15’ on ice ...
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bioRxiv - Immunology 2021Quote: ... The antigenicity and the immunogenicity of the purified recombinant spike protein was validated (Supplementary Fig 1) against verified available recombinant spike protein (BEI Resources, NR52397) and anti-SARS-CoV-2 spike antibody clone A20085C (BioLegend).
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bioRxiv - Immunology 2021Quote: ... or total combined splenocytes plus bone marrow from WT or c-Rel-/- mice were stimulated for 48 hours in complete RPMI containing 10% fetal bovine serum (FBS) and 1% pen strep with either rmIL-2 (1000 U/ mL; BioLegend), rmIL-15 (100 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2023Quote: Supernatants from stimulated T cells were harvested on day 2 and day 6 depending on peak of cytokine production and analyzed using the LEGENDplexTM human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then diluted with 50 μL PBS + 2% FBS and FITC anti-CD45 (BioLegend, 304005; 1:1,000 final dilution) and incubated for 20 min on ice in the dark ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were centrifuged at 500× g at 4 °C and resuspended in 1/3 strength artificial seawater containing 2 μg/mL calcein AM (Enzo) and 100 nM sytox blue (BioLegend) to monitor viability ...
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bioRxiv - Immunology 2023Quote: ... 0.5-1×106 bone marrow cells were stained with the following surface antibodies (2 panels) against: CD14 (Biolegend, M5E2, AF700), HLA-DR (Biolegend ...
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bioRxiv - Developmental Biology 2024Quote: ... and the resulting cDNA was amplified using as PCR oligos the partial TruSeq read 1 adaptor (provided in the 10x kit) and an additive primer (a partial TruSeq read 2, sequence provided by Biolegend and synthesized by Integrated DNA Technologies) ...