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501 - 550 of 1247 citations for 6 Benzyloxy 1H indole 2 boronic acid since 2020

• Isolation, sequence, infectivity and replication kinetics of SARS-CoV-2

  Arinjay Banerjee, et al., bioRxiv - Microbiology 2020

  Quote: ... CD19+ and CD4− CD8− CD19− cells were resuspended in R-10 media (RPMI + 2 mM L-glutamine + 10% FBS + Penicillin/streptomycin) + 20 U/ml IL-2 (Biolegend, https://www.biolegend.com). Primary monocytes were resuspended in R-10 media ...
• Effect of structural stability on endolysosomal degradation and T-cell reactivity of major shrimp allergen tropomyosin

  Sandip D. Kamath, et al., bioRxiv - Immunology 2020

  Quote: ... Culture supernatants thereof were removed and analyzed for IL-2 production as a correlate for T-cell activation using an ELISA MAX mouse IL-2 set (BioLegend, SanDiego, USA).
• A Novel High-Throughput Single B-Cell Cloning Platform for Isolation and Characterization of High-Affinity and potent SARS-CoV-2 Neutralizing Antibodies

  Paritosh Parashar, et al., bioRxiv - Immunology 2022

  Quote: ... Cells were successively stained for 30 minutes on ice with the cocktail of fluorescently conjugated antibodies in 200 μl staining buffer (1X PBS + 2% FBS): 2 μg/ml biotinylated-RBD-Streptavidin-PE and FITC-anti-human IgG antibody (Biolegend, clone: M1310G05) and DAPI ...
• Engineered Flt3L Drives Tolerogenic State to Attenuate Anti-drug Antibody Responses

  Aaron T. Alpar, et al., bioRxiv - Bioengineering 2024

  Quote: ... Blocking occurred using 5% BSA in TBST and antibody probing using 2% BSA in TBST at the indicated dilutions: α-pERK1/2 (Biolegend 369502) 5000x ...
• Glutamine antagonist DRP-104 suppresses tumor growth and enhances response to checkpoint blockade in KEAP1 mutant lung cancer

  Ray Pillai, et al., bioRxiv - Cancer Biology 2023

  Quote: Mice were sedated with ketamine and xylazine and then were injected with 2 ug of APC anti-CD45 (2 ug per mouse diluted in 100 uL PBS, Biolegend 30-F11) retro-orbitally ...
• Single cell transcriptomic analysis of renal allograft rejection reveals novel insights into intragraft TCR clonality

  Tiffany Shi, et al., bioRxiv - Immunology 2023

  Quote: ... Co-culture supernatant was collected following completion of culture and analyzed for IL-2 using the ELISA MAX™ Deluxe Set for human IL-2 (Biolegend, 431804) at a dilution of 1:5.
• Granzyme K drives a newly-intentified pathway of complement activation

  Carlos A. Donado, et al., bioRxiv - Immunology 2024

  Quote: ... cells were sensitized with mouse IgG2a cell-type specific antibodies (HUVEC and THP-1: CD31, HEC7, Invitrogen, 2 μg; Fibroblasts: HLA-A,B,C, W6/32, Biolegend, 2 μg) or a mouse IgG2a isotype control (MG2a-53 ...
• Rac1/ROCK-driven membrane dynamics promote Natural Killer cell cytotoxicity via necroptosis

  Yanting Zhu, Jun Xie, Jue Shi, bioRxiv - Cell Biology 2020

  Quote: ... APC anti-human Nectin-2/CD112 (Clone TX31, Biolegend), APC anti-human PVR/CD155 (Clone SKII.4 ...
• A novel subset of follicular helper-like MAIT cells has capacity for B cell help and antibody production in the mucosa

  Owen Jensen, et al., bioRxiv - Immunology 2021

  Quote: ... mouse anti-human IgD-BV421 (Clone IA6-2, Biolegend) and mouse anti-human PD1-APC (Clone EH1202H7 ...
• Sustained IL-15 response signature predicts RhCMV/SIV vaccine efficacy

  Fredrik Barrenäs, et al., bioRxiv - Genomics 2021

  Quote: ... anti-IL-2 (MQ1-17H12; PE Cy-7; Biolegend), and anti-MIP-1β (D21-1351 ...
• Cyclophilin A regulates protein phase separation and mitigates haematopoietic stem cell aging

  Laure Maneix, et al., bioRxiv - Cell Biology 2021

  Quote: ... and anti-CD45.1/2 (clones A20 and 104, BioLegend) antibodies for 30 min (PB ...
• Molecular determinants and heterogeneity of tissue-resident memory CD8⁺ T lymphocytes revealed by single-cell RNA sequencing

  Nadia S. Kurd, et al., bioRxiv - Immunology 2020

  Quote: ... and IL-2 (JES6-5H4) antibodies (all from Biolegend) for 30 minutes on ice ...
• Efficacy and breadth of adjuvanted SARS-CoV-2 receptor-binding domain nanoparticle vaccine in macaques

  Hannah A. D. King, et al., bioRxiv - Immunology 2021

  Quote: ... IL-2-BV750 (MQ1-17H12, BioLegend #566361, Lot 0042313), IL-4 BB700 (MP4-25D2 ...
• Neisseria gonorrhoeae subverts formin-dependent actin polymerization to colonize human macrophages

  Stanimir S. Ivanov, et al., bioRxiv - Microbiology 2021

  Quote: ... Anti-human LAMP-2 (H4B4) was purchased from BioLegend. Anti-DIAPH2 (A300-079A-T ...
• Rapid protection from COVID-19 in nonhuman primates vaccinated intramuscularly but not intranasally with a single dose of a recombinant vaccine

  Wakako Furuyama, et al., bioRxiv - Microbiology 2021

  Quote: ... IL-2-PerCPCy5.5 diluted in perm-wash buffer (Biolegend). For analysis of NK cell intracellular cytokine production ...
• CARD8 inflammasome sensitization through DPP9 inhibition enhances NNRTI-triggered killing of HIV-1-infected cells

  Kolin Clark, Qiankun Wang, Liang Shan, bioRxiv - Microbiology 2021

  Quote: ... antibody and 20 ng/ml IL-2 (Biolegend #589106) for 3 days ...
• A rapid and efficient screening system for neutralizing antibodies and its application for the discovery of potent neutralizing antibodies to SARS-CoV-2 S-RBD

  Xiaojian Han, et al., bioRxiv - Immunology 2020

  Quote: ... BV421-anti-human IgD antibody (Biolegend, clone: IA6-2), PerCP-Cy5.5-anti-human IgG antibody (Biolegend ...
• Pharmacological tuning of microRNAs in FAP-derived Extracellular Vesicles by HDAC inhibitors promotes regeneration and reduces fibrosis in dystrophic muscles

  M. Sandonà, et al., bioRxiv - Cell Biology 2020

  Quote: ... rat anti-CD63 PE (#143904, clone NUG-2, Biolegend) and Myeloperoxidase/MPO (1/100 #MAB3174 ...
• Antibodies to ILT3 abrogate myeloid immunosuppression and enable tumor killing

  Philip E. Brandish, et al., bioRxiv - Cancer Biology 2021

  Quote: ... CD45 (AF700, Biolegend, #304024, clone HI30, 2 uL, human), CD56 (BV605 ...
• Modest changes in Spi1 dosage reveal the potential for altered microglial function as seen in Alzheimer’s disease

  Ruth E. Jones, et al., bioRxiv - Neuroscience 2021

  Quote: ... Anti-CD11b BV421 (M1/70, 2 μg/mL, Biolegend), Anti-CD11b PerCP-Cy5.5 (M1/70 ...
• BET Inhibition Enhances TNF Mediated Anti-Tumor Immunity

  Lisa C. Wellinger, et al., bioRxiv - Cancer Biology 2021

  Quote: ... and 100 IU/mL recombinant human IL-2 (Biolegend) in RPMI media supplemented with 10% FBS ...
• Endophilin A2 regulates B cell protein trafficking and humoral responses

  Dessislava Malinova, et al., bioRxiv - Immunology 2020

  Quote: ... CD138 (281-2) and c-kit (2B8) from BioLegend.
• Direct tissue sensing reprograms TLR4⁺ Tfh-like cells inflammatory profile in the joints of rheumatoid arthritis patients

  Daniela Amaral-Silva, et al., bioRxiv - Immunology 2021

  Quote: ... and 2 μg/mL of anti-ICOS (C398.4A, BioLegend), crosslinked with 5 μg/mL anti-mouse IgG1 (BioLegend ...
• Hemoglobin drives inflammation and initiates antigen spread and nephritis in lupus

  Hritika Sharma, Anjali Bose, Uma Kumar, Rahul Pal, bioRxiv - Immunology 2020

  Quote: ... cells were incubated with Fc block (2 µg, Biolegend). LIVE/DEAD™ fixable violet (Thermo Fisher Scientific ...
• Cytotoxic efficiency of human CD8⁺ T cell memory subtypes

  Arne Knörck, et al., bioRxiv - Immunology 2021

  Quote: ... or PE-conjugated anti-human CD253 (RIK-2, Biolegend) was performed ...
• Circulating levels of epirubicin cause endothelial senescence while compromising metabolic activity and vascular function

  Amanda J Eakin, et al., bioRxiv - Cell Biology 2020

  Quote: ... rat anti-mouse Mac-2 (Galectin-3, 125402, Biolegend) for myeloid cells (Ho and Springer 1982) ...
• Type 1 interferon remodels normal and neoplastic hematopoiesis in human

  Chhiring Lama, et al., bioRxiv - Cancer Biology 2022

  Quote: ... 2 or 3 (TotalSeq-A, clone LNH-94, Biolegend) for GoT-IM ...
• Development of SARS-CoV-2 mRNA vaccines encoding spike N-terminal and receptor binding domains

  Guillaume B.E. Stewart-Jones, et al., bioRxiv - Immunology 2022

  Quote: ... IL-2 BV711 (BioLegend, cat. 503837, clone JES6-5H4), IL-4 PE-Cy7 (BioLegend ...
• A multi-enhancer hub at the Ets1 locus controls T cell differentiation and allergic inflammation through 3D genome topology

  Aditi Chandra, et al., bioRxiv - Genomics 2022

  Quote: ... 2 ng/mL of recombinant TGFβ (BioLegend, Cat#763102), and 20 ng/mL of recombinant IL-6 (BioLegend ...
• Excess BAFF Alters NR4As Expression Levels and Breg Function of Human Precursor-like Marginal Zone B-cells in the Context of HIV-1 infection

  Kim Doyon-Laliberté, et al., bioRxiv - Immunology 2022

  Quote: ... on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 96 well plates with soluble anti-CD28 (2 μg/mL ...
• A SARS-CoV-2 Spike Ferritin Nanoparticle Vaccine is Protective and Promotes a Strong Immunological Response in the Cynomolgus Macaque Coronavirus Disease 2019 (COVID-19) Model

  Sara C. Johnston, et al., bioRxiv - Microbiology 2022

  Quote: ... IL-2-BV750 (MQ1-17H12, BioLegend #566361, Lot 0042313), IL-4 BB700 (MP4-25D2 ...
• Extrafollicular responses are sufficient to drive initiation of autoimmunity and early disease hallmarks of lupus

  Lasse F. Voss, et al., bioRxiv - Immunology 2022

  Quote: ... CD138-PE clone 281-2 (142504, BioLegend, 1:500), CD169-PE clone 3D6.112 (142404 ...
• Pink1-mediated mitophagy in the endothelium releases proteins encoded by mitochondrial DNA and activates neutrophil responses

  Priyanka Gajwani, et al., bioRxiv - Cell Biology 2022

  Quote: ... Cells were fixed in 2% Fixation buffer (Biolegend 420801) for 10 minutes ...
• Quantitative effects of co-culture on T cell motility and cancer-T cell interactions

  Xinyue Li, et al., bioRxiv - Systems Biology 2024

  Quote: ... and 30 U/ml mouse recombinant IL-2 (Biolegend).
• Spermidine/spermine N1-acetyltransferase controls tissue-specific regulatory T cell function in chronic inflammation

  Teresa Neuwirth, et al., bioRxiv - Immunology 2024

  Quote: ... anti-IL-2 PE (Biolegend, Cat: 340450, 1:50), anti-IL-17A APC (eBioscience ...
• Targeting GFPT2 to reinvigorate immunotherapy in EGFR-mutated NSCLC

  Luyao Ao, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Panel 2: BV421-conjugated CD45 (1:100, 103133, Biolegend), PE/Cy7-conjugated CD3 (1:100 ...
• The role of B cells in immune cell activation in polycystic ovary syndrome

  Angelo Ascani, et al., bioRxiv - Immunology 2023

  Quote: ... CD138-PE/Cyanine7 (Syndecan-1, clone 281-2, BioLegend), CD27-APC (clone LG.3A10 ...
• CTLA-4 tail fusion enhances CAR-T anti-tumor immunity

  Xiaoyu Zhou, et al., bioRxiv - Immunology 2023

  Quote: ... followed by Anti- ERK1/2 (pT202/pY204)-Alexaflour647 (Biolegend) staining at room temperature for 30 minutes ...
• The CARD8 inflammasome drives CD4⁺ T-cell depletion in HIV-1 infection

  Qiankun Wang, Liang Shan, bioRxiv - Microbiology 2023

  Quote: ... Cytokines used in this study: IL-2 (BioLegend #589106), IL-7 (BioLegend #581904) ...
• Enhancing anti-EGFRvIII CAR T cell therapy against glioblastoma with a paracrine SIRPγ-derived CD47 blocker

  Tomás A. Martins, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and (2) anti-NESTIN (clone 10C2; #656802, BioLegend, USA) and anti-EGFRvIII (clone RM419 ...
• Generation of antigen-specific memory CD4 T cells by heterologous immunization enhances the magnitude of the germinal center response upon influenza infection

  Linda M. Sircy, et al., bioRxiv - Immunology 2023

  Quote: ... CD138 (281–2) (purchased from BD Biosciences, eBiosciences, BioLegend, Vector Laboratories Inc. ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... IL-2 (1:100 dilution; clone JES6-5H4, BioLegend) and anti-IL-4 (1:100 dilution ...
• Targets and cross-reactivity of human T cell recognition of Common Cold Coronaviruses

  Alison Tarke, et al., bioRxiv - Immunology 2023

  Quote: ... and OX40 PE-Cy7 (2:100; Biolegend Cat#350012). All samples were acquired on ZE5 cell analyzer (Bio-rad laboratories ...
• Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies

  Katrin Fischer, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD138 BV785 (1:80, clone 281-2, BioLegend) for 30 min on ice and then washed once in FACS buffer ...
• CD38 regulates ovarian function and fecundity via NAD⁺ metabolism

  Rosalba Perrone, et al., bioRxiv - Developmental Biology 2023

  Quote: ... CD138-BV785 (BioLegend, clone 281-2, 142534, 1:800), CD38-Pac Blue (BioLegend ...
• Generation of circulating autoreactive pre-plasma cells fueled by naïve B cells in celiac disease

  Ida Lindeman, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human IgD-PerCP/Cy5.5 (clone IA6-2, BioLegend), anti-human IgD-Alexa Fluor 700 (clone IA6-2 ...
• Opposing diet, microbiome and metabolite mechanisms regulate inflammatory bowel disease in a genetically susceptible host

  Gabriel Vasconcelos Pereira, et al., bioRxiv - Microbiology 2023

  Quote: ... APC-conjugated anti-CD138 (Biolegend, 281-2; 1/100), FITC-conjugated anti-IgA (eBiosciences ...
• The extrafollicular B cell response is a hallmark of childhood idiopathic nephrotic syndrome

  Tho-Alfakar Al-Aubodah, et al., bioRxiv - Immunology 2023

  Quote: ... anti-human IgD BV510 (1:20, IA6-2, BioLegend), anti-human IgM Alexa Fluor 488 (1:40 ...
• Collagen receptor GPVI-mediated platelet activation and pro-coagulant activity aggravates inflammation and aortic wall remodelling in abdominal aortic aneurysm

  T Feige, et al., bioRxiv - Cell Biology 2023

  Quote: ... macrophages or granulocytes (CD14, Sa14-2-APC, #123311, BioLegend and CD11b ...
• Epigenetic control of CD8⁺ T cell tissue homing and tissue resident memory T cell precursors by the histone methyltransferase SUV39H1

  Guadalupe Suarez, et al., bioRxiv - Immunology 2024

  Quote: ... PE/Cyanine7 anti-mouse CD49d Antibody (R1-2, Biolegend), PE-Cy7 Rat Anti-Mouse CD62L (MEL-14 ...