Labshake search
Citations for BioLegend :
501 - 550 of 595 citations for 10 PROPOXY DECANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... Cells were pelleted by centrifugation and then resuspended in 100 μL FACs buffer (PBS with 10 % FCS) with PE-conjugated anti-CD61 (RRID:AB_313084, Biolegend, 104307, 1:200) and Alexa Fluor 647-conjugated anti-SSEA1 (RRID:AB_1210551 ...
-
bioRxiv - Immunology 2019Quote: ... cells were permeabilized and stained with IL-10 (clone JES5-16E3, eBioscience) or isotype control (Rat IgG2b Κ Isotype Control, Biolegend). Fluorescence intensities were measured on a FACSCalibur and at least 25,000 events for lung tissue were analyzed using FlowJo.
-
bioRxiv - Immunology 2021Quote: Mouse B cells were bulk sorted from mixed chimeras into Eppendorf tubes containing cold sterile PBS 10% FCS and incubated for 20 min at 4°C with TotalSeq™ DNA-barcoded anti-mouse ‘Hashing’ antibodies (BioLegend) at a 1/100 final dilution ...
-
bioRxiv - Molecular Biology 2019Quote: ... and coupled with 10 μg carrier-free anti-epitope tag antibody (c-myc: Santa Cruz #sc-40; FLAG: Sigma #F1804; GluGlu: Biolegend #901801 ...
-
bioRxiv - Cell Biology 2019Quote: ... insulin-like growth factor 2 (IGF2; 20 ng/ml) and fibroblast growth factor (FGF; 10 ng/ml) (BioLegend or StemCell Technologies) along with 5ug/ml polybrene (Sigma ...
-
bioRxiv - Cancer Biology 2019Quote: ... The cells were washed with HBSS twice by centrifugation at 250 x g for 8 min and then suspended in BMDC culture medium (RPMI-1640 medium [Thermo Fisher Scientific] containing 10% FBS, 20 ng/ml recombinant mouse GM-CSF [BioLegend] ...
-
bioRxiv - Microbiology 2021Quote: ... and resuspended in RPMI with 10% FBS medium and were stimulated for 6 h with a cell activation cocktail containing PMA and ionomycin (Biolegend, # 423302) in the presence of protein transport inhibitor ...
-
bioRxiv - Biophysics 2021Quote: ... The chambers were then incubated with 10 μg/ml purified Anti-HA 11 Epitope tag antibody (BioLegend, San Diego, CA, USA) in BIB at room temperature for 1 hour ...
-
bioRxiv - Microbiology 2020Quote: ... mouse monoclonal anti-DnaK 4RA2 at 1□:□10,□000 dilution (Enzo, ADI-SPA-880) and HRP Goat anti-mouse IgG (BioLegend, 405306) at 1□:□10,□000 dilution ...
-
bioRxiv - Cell Biology 2021Quote: ... WT and CD56 KO NK92 and YTS cells were incubated for 20 mins at 37°C and 5% CO2 on .5 glass coverslips that had been pre-coated with 10 μg/ml anti-CD18 (clone IB4) and anti-NKp30 (clone P30-15, Biolegend, NK92) or anti-CD28 (clone CD28.2 ...
-
bioRxiv - Immunology 2021Quote: ... permeabilized with saponin 0.2% for 20 min and stained with PE-Cy7-anti-IFN-γ mAb and/or PE-anti-IL-10 mAbs (Biolegend). For TF and GzB/PRF staining ...
-
bioRxiv - Microbiology 2021Quote: ... followed by washing and incubated with a solution of AlexaFluor® 647 conjugated anti-His antibody (BioLegend #652513, 10 µg/mL). After washing and drying ...
-
bioRxiv - Microbiology 2019Quote: ... Cells were harvested using Trypsin-EDTA and resuspended in FACS buffer (PBS containing 10% FBS and 2mM EDTA) and stained with anti-CD300lf PE clone Tx70 (1:100; BioLegend, #132704) or anti-CD300ld PE clone Tx69 (1:100 ...
-
bioRxiv - Cancer Biology 2020Quote: ... were treated with 10 Gy (as described above) and either PMX205 or vehicle and either IL-10 blocking antibody (LEAF Purified clone JES5-16E3, Biolegend #505012) (50 μg 24 hours before irradiation ...
-
bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
-
bioRxiv - Immunology 2020Quote: ... were added to the tumor cells and incubated for 2 hours with CD20-TCB (200 ng/ml) and +/- αLFA1 antibody (Clone: TS 1/18, 10 μg/ml, BioLegend, 302116). Cells were then gently washed ...
-
bioRxiv - Microbiology 2021Quote: ... were injected intravenously into the tail vein with 10-15 μg of Alexa Fluor™ 647-coupled anti-CD31 antibody (clone 390, Biolegend) to label blood vessels ...
-
bioRxiv - Microbiology 2020Quote: ... Splenic cell suspensions were treated additionally with red blood cell lysis buffer at room temperature for 10 min (Sigma-Aldrichor BioLegend Inc) for removing RBCs to obtain single cell suspensions ...
-
bioRxiv - Cell Biology 2021Quote: ... the cell pellets washed twice with 10% FCS in PBS (100μl/well) and then resuspended in 10% FCS in PBS (100μl/well) containing APC/Cyanine7 Goat anti-mouse IgG (Biolegend; 1:250) and incubated on ice for 30mins ...
-
bioRxiv - Immunology 2019Quote: ... The following ELISA kits were used according to manufacturer recommendations: ELISA Max human ELISA for IL-10 (Biolegend, San Diego, CA), anti-pan human IFN alpha (Interferon Source ...
-
bioRxiv - Developmental Biology 2020Quote: Cells obtained from embryos and stained with Epha1 antibodies as described above were then washed twice with PBS/10% DS and processed for staining using True-NuclearTM transcription factor Buffer Set (BioLegend #424401) according to the manufacturer’s instructions and following the protocol described in (Aires et al. ...
-
bioRxiv - Immunology 2020Quote: ... TnT cells and Jurkat-derived cell lines were stimulated overnight with either 10 μg ml−1 plate-bound anti-human CD3e antibody (clone OKT3, BioLegend, #317326) or 1X eBioscience Cell Stimulation Cocktail (81 nM PMA ...
-
bioRxiv - Immunology 2021Quote: ... Cells were stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend) and washed once in FACS buffer ...
-
bioRxiv - Bioengineering 2021Quote: ... and IL-10 were analyzed using a human COVID-19 Cytokine Storm Panel 1 (13-plex) kit (Cat. No. 741091; BioLegend, USA) according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2021Quote: ... Monocyte isolation was determined with the following flow cytometry antibody panel after 10 min incubation with anti-human CD32 (StemCell – 18520): APC anti-human CD14 (Biolegend – 301808), PE-Cy7 anti-human CD3 (Biolegend – 344186) ...
-
bioRxiv - Immunology 2021Quote: ... were co-cultured with 1×105 relevant target cells (10:1 E:T ratio) overnight in the presence of Brefeldin A (BFA) (Biolegend, CA, USA), and the next day ...
-
bioRxiv - Immunology 2021Quote: ... The following flow cytometry antibody panel was used to determine monocyte differentiation and M1 polarization after 10 min incubation with anti-human CD32: APC anti-human CD14 (Biolegend – 344186) BV421 anti-human CD80 (Biolegend – 305222) ...
-
bioRxiv - Immunology 2021Quote: Leukocytes from whole blood and ETA samples were incubated with oligo-conjugated Ig-A/D/G/M for 10 min at 4°C followed by the addition of Human TruStain FcX™ (BioLegend) and 10 min incubation at RT ...
-
bioRxiv - Microbiology 2022Quote: ... BMDMs were exposed to their indicated treatment condition in complete RPMI overnight (∼18 hours): IFNγ stimulation (10 ng/mL; BioLegend, 575304); supplementation with pyruvate or nicotinamide (NAM ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were permeabilized for intra-cellular staining with a saponin-based buffer (1% saponin, 10% BSA in PBS) and stained with an antibody for Cytochrome C AlexaFluor 647 (Biolegend, 612310) used at 1:2000 dilution and DAPI ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were permeabilized for intra-cellular staining with a saponin-based buffer (1% saponin, 10% BSA in PBS) and stained with an antibody for Cytochrome C AlexaFluor 647 (Biolegend, 612310) used at 1:2000 dilution and DAPI ...
-
bioRxiv - Genomics 2022Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...
-
bioRxiv - Cancer Biology 2022Quote: ... for 10 min at 4°C followed by an incubation with CD45-BV421 (Biolegend 103133, clone 30-F11, 1:200 dilution) and Ter119-BV421 (Biolegend 116233 ...
-
bioRxiv - Cancer Biology 2022Quote: Oligo-labeled antibody staining was done on both melanoblasts and melanom cells using the TotalSeq™-A Antibodies and Cell Hashing with 10× Single Cell 3′ Reagent Kit (Biolegend). The HashTag Oligonucleotidess used for the analysis are mHTO01 ...
-
bioRxiv - Cancer Biology 2022Quote: ... spun at 700 g for 3 minutes at 4°C and washed in MACS Buffer 3× before being incubated with the following antibodies: APC-conjugated rat anti-CD3 (5 μL/10×106 cells; Biolegend, 100408), PE-conjugated rat anti-CD8b (2.5 μL/10×106 cells ...
-
bioRxiv - Immunology 2023Quote: ... The sample pool was washed once with 10 mL of PBS-BSA and resuspended in 150 µL of Zombie NIR viability dye (1:500 in PBS; BioLegend 423105). After 15 minutes on ice ...
-
bioRxiv - Neuroscience 2023Quote: ... leptomeninges and brain cells were pelleted at 300xg for 10 minutes and incubated with anti-CD11b APC/Cy7 (BioLegend cat. #101226) and anti-CD45 BV510 (BD Biosciences cat ...
-
bioRxiv - Cancer Biology 2022Quote: ... LEPs from co-cultures were separated after 10 days for gene expression qPCR analysis by FACS using anti-human CD133-PE (BioLegend, clone7) and anti-human CD271-APC (BioLegend ...
-
bioRxiv - Cancer Biology 2023Quote: ... 500,000 cells were resuspended in PBS + 1% FBS and spun at 300g for 10 minutes at 4°C and then resuspended in 25 μL staining buffer (BioLegend #420201). 2.5 μL human TruStain FcX (BioLegend #42230 ...
-
bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions) were incubated with 10 nM of a second CD98hc antibody for detection (RL388, rat anti-mouse antibody; BioLegend, 128202). Non-treated cells were also incubated with the TfR-1 (R17 ...
-
bioRxiv - Immunology 2023Quote: ... stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend), and washed once in FACS buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... Collected cell populations were immediately seeded in tissue culture at a concentration of 2x105 cells per 1.9 cm2 in DMEM containing 5% FBS and 10 ng/mL TGFβ (Biolegend Cat 763102) or 500 nM Prostaglandin F (Cayman Chemical Cat 16010).
-
bioRxiv - Cancer Biology 2023Quote: ... T cells transfected with pMAX-GFP and either pcDNA3.1-Rai or empty vector were added in a 10:1 ratio and incubated for 4 h in presence of APC anti-LAMP1 antibody (clone H4A3, Biolegend #328620). Monensin (2mM ...
-
bioRxiv - Physiology 2023Quote: ... Cisplatin is washed off and non-specific binding was blocked with a 10-minute staining of cells with Fc Block (Biolegend; 1µg). Cells were stained with 0.5µg of cell surface metal-conjugated antibodies for 30 minutes at room temperature ...
-
CD56/NCAM mediates cell migration of human NK cells by promoting integrin-mediated adhesion turnoverbioRxiv - Cell Biology 2023Quote: ... at 37°C and 5% CO2 on #1.5 glass coverslips that had been pre-coated with 10 µg/ml anti-CD18 (clone IB4) and anti-NKp30 (clone P30-15, Biolegend; 325202) or 5 µg/ml Fc-ICAM-1 (Enzo Life Science ...
-
bioRxiv - Immunology 2024Quote: ... that were pulsed with epitope (10 ng/mL) was collected and IFN-γ production was measured with an enzyme-linked immunosorbent assay (ELISA, BioLegend) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... Splenocytes from naive or tumor-bearing AST;Cre-ERT2 mice were mixed with 2×106 C57BL/6 splenocytes and incubated in RPMI-10 for 4 hours at 37°C in the presence of brefeldin A (Biolegend 420601) and TAG peptide (SAINNYAQKL [0.5 μM] ...
-
bioRxiv - Molecular Biology 2021Quote: ... the cells were incubated for 10 min at RT in 1.0 μg anti-mouse CD16/32 antibody (BioLegend, San Diego, CA, USA) containing 500 μl FACS buffer for Fc receptor blocking ...
-
bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...