Labshake search
Citations for BioLegend :
5251 - 5300 of 5958 citations for Chondroitin Sulfate Proteoglycan 4 CSPG4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: For FACS analysis the following antibodies were used: anti-CD11c APC and anti-I-A/I-E APC-Cy7 (Biolegend), anti-CD40 PeCy7 ...
-
bioRxiv - Immunology 2021Quote: ... The antigenicity and the immunogenicity of the purified recombinant spike protein was validated (Supplementary Fig 1) against verified available recombinant spike protein (BEI Resources, NR52397) and anti-SARS-CoV-2 spike antibody clone A20085C (BioLegend).
-
bioRxiv - Immunology 2021Quote: ... Single cell suspensions were blocked with rat anti-mouse FcγIII/II receptor (CD16/CD32) blocking antibodies (“Fc-Block”) and stained with live/dead cell-exclusion dye (Zombie UV dye; Biolegend). The cells were then incubated with fluorophore-conjugated antibodies directed against cell surface antigens ...
-
bioRxiv - Immunology 2021Quote: ... The plates were washed twice in FACS buffer and stained with 50 μl/well of 1:200 dilution of PE-conjugated mouse anti-human IgG Fc antibody (BioLegend) on ice in dark for 1h ...
-
bioRxiv - Immunology 2021Quote: ... fluorochrome-conjugated antibodies (anti-mouse CD45-BioLegend Cat. No. 103126, anti-human CD45-BioLegend Cat. No. 304014, anti-human CD3-BioLegend Cat ...
-
bioRxiv - Molecular Biology 2021Quote: ... CD4+ or CD8+ cells were stained with an APC-Cy7-labeled anti-CD4 and FITC-labeled anti-CD8 antibody (Biolegend), respectively ...
-
bioRxiv - Immunology 2020Quote: ... Anti-CD69 (H1.2F3, PE/Cy7, B253212, 104512), Anti-CD3 (17A2, APC/Cyanine7, B283940, 100222) antibodies were purchased from BioLegend (Bxxxxxx) or BD Biosciences (xxxxxxx).
-
bioRxiv - Immunology 2021Quote: ... from Miltenyi according to the manufacturer’s instructions and cultured in the above medium supplemented with 2 µg/ml anti-human RP105 antibody clone (312907, BioLegend).
-
bioRxiv - Immunology 2020Quote: ... ABCs were isolated from purified total B cells by CD11c positive selection using a biotin conjugated CD11c antibody (301612, Biolegend) and anti-biotin magnetic beads ...
-
bioRxiv - Immunology 2020Quote: ... buffer containing 1:100 dilution of target-specific antibodies (anti PD1:cat≠329905; Biolegend, and anti-PDL1: Cat≠329705; Biolegend) and incubated for 30 minutes at 4°C in the dark ...
-
bioRxiv - Immunology 2020Quote: ... The pellet was resuspended in flow cytometry staining buffer (FACS) buffer containing 1:100 dilution of CD3 antibody (Cat≠317307; Biolegend) and incubated for 30 minutes at 4°C in the dark ...
-
bioRxiv - Immunology 2021Quote: ... for 15 min at RT and stained intracellularly with Fix/Perm Buffer B and antibodies for TNF (PE-Cy7, clone MAb11, BioLegend), IFN-γ (BV711 ...
-
bioRxiv - Immunology 2021Quote: ... and finally with a mixture of AlexaFluor 647-conjugated rat anti-mouse Ly6C antibody (BioLegend, clone HK1.4; diluted 1:500) and DAPI (Molecular Probes ...
-
bioRxiv - Immunology 2020Quote: ... for 5 minutes at room temperature prior to staining with a surface-stain cocktail containing the following antibodies purchased from BioLegend® (San Diego ...
-
bioRxiv - Immunology 2021Quote: ... Cells were then blocked with human Fc-block (Miltenyi) in the presence or absence of purified anti-CD36 antibody (Clone 5-271; Biolegend) at 10 μg/ml for a further 15 min at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... PD-1 and CD25 expression and cell viability and cell expansion were quantified by live/dead staining and the following antibodies via flow cytometry: anti-TCRa/b-PE (306708, BioLegend), anti-CD3-AF700 (11-0039-42 ...
-
bioRxiv - Immunology 2020Quote: ... PerCP-conjugated anti-CD11c (N418) and Pacific-Blue-conjugated anti-CD19 (6D5) antibodies were obtained from Biolegend (San Diego, USA). The APC-conjugated anti-CD154 antibody was obtained from Miltenyi Biotec ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were transferred to a round-bottomed 96-well plate and were incubated with an AF647-conjugated anti-GFP antibody (1:20, BioLegend) and fixable viability dye eFluor 780 (1:1000 ...
-
bioRxiv - Immunology 2021Quote: ... Cells were then washed in 1x eBioscience Permeabilization Buffer twice and incubated with indicated intracellular antibodies including anti-TNF-PE (MAb1) and anti-IFN-γ-APC (4S.B3) from BioLegend at room temperature for 1 hour ...
-
bioRxiv - Genomics 2021Quote: ... Then cells were stained with the following cell surface marker antibodies for 30 min on ice (1:100 dilution, all from BioLegend): CD11b-PE (301306 ...
-
bioRxiv - Microbiology 2021Quote: ... prior to staining cells in 100 µL perm/wash with 0.5 µL APC anti-human IL-6 antibody (Cat# 501112, Biolegend®) overnight at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... washed twice in buffer (PBS + 0.5% BSA) and stained with the anti-FLAG-phycoerythrin (PE) antibody (anti-DYKDDDDK Tag, BioLegend 637310) for confirmation of scFv expression and RSV-F protein conjugated with Alexa Fluor 647 (AF647 ...
-
bioRxiv - Cancer Biology 2020Quote: Flow cytometry staining was performed using the antibodies in Supplementary Table 2 in addition to the Zombie Aqua viability stain (BioLegend) and the Foxp3 Transcription Factor Staining Buffer Set (eBioscience) ...
-
bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (1% BSA in PBS) at a concentration of 108 cells/mL and 1 μL negative CD8+ T cell biotin-antibody cocktail (Biolegend)/million cells was added and allowed to incubate for 15 min on ice ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with PBS followed by surface staining at 4 °C with directly conjugated antibodies (see list below) diluted in FACS buffer containing anti-CD16/32 (BioXCell, BE0307, RRID:AB_2736987 or BioLegend, 101320, RRID:AB_1574975) for 30 min ...
-
bioRxiv - Cell Biology 2022Quote: ... Co-cultures were fixed at 7-10 days in either 4% paraformaldehyde for 10 minutes or ice-cold 70% ethanol for 30 minutes prior to staining with anti-CD31-AF488-conjugated antibody (BioLegend) and anti-VWF (DAKO ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were then processed for single cell RNAseq as described in (Peng et al., 2019) using Total Seq B antibodies and the recommended protocol from Biolegend. Briefly ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 1×PBS) mixed with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:200, Clone: 93, BioLegend, CA, USA) and eBioscience™ Fixable Viability Dye eFluor™ 780 (1:1000 ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein levels of overexpressed PRT6-WT and PRT6-136H+161V were determined by Western blot using anti-HA antibody (16B12, BioLegend).
-
bioRxiv - Cancer Biology 2022Quote: ... and activated using plate-bound anti-CD3 (1μg/ml; clone-145-2C11) and anti-CD28 (5μg/ml; clone-37.51) antibodies (Biolegend; San Diego, CA) for 4 days.
-
bioRxiv - Immunology 2022Quote: ... Cells were then stained with Cell Viability Dye eFluor506 (eBioscience) and anti-CD45 antibody Pacific blue (clone 30-F11, Biolegend). CD45+ live cells were then sorted using BD Aria cell sorter.
-
bioRxiv - Immunology 2022Quote: ... Each of the three biological replicates that were treated with PBS vehicle control or TGM was labeled with one of three unique TotalSeq Hashtags Antibody markers (Biolegend). The samples were individually sort-purified by gating CD45+ cells and then each treatment group was pooled with an even number of cells from each sample ...
-
bioRxiv - Immunology 2022Quote: ... They were then stained overnight at 4ºC with the following antibodies: α-smooth muscle actin (αSMA for myofibroblast, AF488 1:100, Biolegend). Following primary staining ...
-
bioRxiv - Immunology 2022Quote: ... Cells from each sample were stained individually for 30 mins with a master mix containing the antibodies specific to CD45-FITC (1:100, Biolegend) and a TotalSeq Hashtags Antibody marker (Biolegend) ...
-
bioRxiv - Immunology 2022Quote: ... and then stained for 30 min on ice with the following antibodies in flow cytometry staining buffer (FACS): anti-CD4 AF589 (clone GK1.5; Biolegend #100446), anti-CD8b (clone YTS156.7.7 ...
-
bioRxiv - Immunology 2022Quote: ... Single-cell libraries were prepared using the single Cell 3’ Kit v 3.1 following a modified 3’ v3.1 assay protocol (User Guide CG000206) to concurrently prepare gene expression and TotalSeq A antibody derived tag (ADT) libraries as recommended by BioLegend. 1 ul of 0.2uM ADT Additive Primer (CCTTGGCACCCGAGAATT*C*C ...
-
bioRxiv - Neuroscience 2022Quote: ... Retinas were then placed in primary antibody solution containing mouse anti-SMI-32 (1:1000, BioLegend, Cat#801701, RRID: AB_509997) in blocking solution for 2-4 days at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... Samples sorted for 10x Genomics single-cell RNAseq were individually labelled with an oligonucleotide-linked hashing antibody (Totalseq-C, Biolegend) in addition to the staining cocktail and sorted into a single tube according to the gating strategy shown in Fig ...
-
bioRxiv - Microbiology 2022Quote: ... the BrdU-labeled splenocytes were surface stained for T-cell (CD3e-APC; eBioscience) marker after blocking with anti-mouse CD16/32 antibodies (BioLegend). Cells were then permeabilized and treated with DNase to expose BrdU epitopes followed by anti-BrdU-FITC and 7-AAD (7-amino-actinomycin D ...
-
bioRxiv - Immunology 2022Quote: ... The membrane was washed three times with TBS-T for 10 min and subjected to the HRP-conjugated secondary goat anti-mouse antibody (Biolegend) in a solution of 3% non-fat milk in TBS-T for 1 h ...
-
bioRxiv - Immunology 2022Quote: Splenocytes isolated from four female mice (two Ezh2fl/fl and two CD19-Cre Ezh2fl/fl) were stained with BV421 anti-mouse CD19 antibody (Clone 6D5, BioLegend) and live/dead dye ...
-
bioRxiv - Immunology 2022Quote: ... CD4- population for calculating MAIT cell percentage in PBMC was determined by gating out CD4+ cells stained with CD4-BV605 antibody (Biolegend). CAR CD8+ T cells were identified with CD3-APC/Cy7 or CD8-Alexa 700 antibodies (Biolegend) ...
-
bioRxiv - Microbiology 2022Quote: ... cells were stained in PBS with 0.5% BSA with Fixable Viability Dye eFluor 780 (eBioscience) and a combination of the following antibodies: anti-mouse CD45 (PE/Cy5, 30-F11, BioLegend), CD45 (Alexa Fluor 700 ...
-
bioRxiv - Immunology 2022Quote: The following antibodies were used to detect B cell populations in the BM: brilliant violet (BV) 421 anti-mouse CD19 antibody (Clone 6D5, BioLegend), PE/Cyanine5 anti-mouse/human CD45R/B220 (Clone RA3-6B2 ...
-
bioRxiv - Immunology 2022Quote: ... Splenic B cells from each mouse were incubated with a unique TotalSeq-C0301 anti-mouse hashtag antibody conjugated to a Feature Barcode oligonucleotide (BioLegend) to label each mouse at 4°C in 100 μl PBS+0.04% BSA for 30 minutes ...
-
bioRxiv - Immunology 2022Quote: ... Single cell suspensions were stained for 45 minutes to an hour at room temperature with the following antibodies: TruStain FcX PLUS (S17011E, BioLegend), BUV496-labeled CD45 (30-F11 ...
-
bioRxiv - Immunology 2022Quote: ... All post-enrichment antibody staining was performed on ice for 45 minutes in the presence of True-Stain Monocyte Blocker (BioLegend). Following staining ...
-
bioRxiv - Immunology 2022Quote: ... and then stained with the appropriate extracellular antibodies (anti-CD45, clone H130, Biolegend; anti-CD3, clone HIT3a, Biolegend; anti-CD4, clone RPA-T4, Biolegend; anti-CD45RA ...
-
bioRxiv - Immunology 2022Quote: Circulating CD20+ cells were depleted by intraperitoneal administration of 200 μg of rat-anti-mouse-CD20-depleting antibody (Biolegend, 152104) two days before the start of TAM administration ...
-
bioRxiv - Molecular Biology 2022Quote: ... as well as antibodies to cell surface markers (anti-45 AF700 [Biolegend, 147716], anti-CD3e FITC [Biolegend, 100306], anti-CD4 BV786 [Biolegend, 100552] ...