Rat WIF 1 ELISA Kit citations for BioLegend

A mass cytometry approach to track the evolution of T cell responses during infection and immunotherapy by paired T cell receptor repertoire and T cell differentiation state analysis

Jesse Garcia Castillo, et al., bioRxiv - Immunology 2024

Quote: ... Fixation/permeabilization of cells was conducted for intracellular staining using the eBioscience Foxp3 fixation/permeabilization kit (BioLegend) or Tonbo Foxp3 / Transcription Factor Staining Buffer Kit.

Riluzole suppresses growth and enhances response to endocrine therapy in ER+ breast cancer

Ayodeji O. Olukoya, et al., bioRxiv - Cancer Biology 2023

Quote: ... The PI/Annexin V-FITC apoptosis detection kit was purchased from BioLegend (#640914, San Diego, CA, USA). Data are presented as mean ± SD for 3 (SUM44 ...

Integration of Avidity and Differentiation is enabled by CD8⁺ T-cell sensing of IFN-γ

Lion F.K. Uhl, et al., bioRxiv - Immunology 2023

Quote: ... Live dead staining and surface staining was performed using Zombie NIR Fixable Viability Kit (Biolegend, #423106/423105), TruStain FcX™ (anti-mouse CD16/32 ...

GSK3α/β restrains IFNγ-inducible costimulatory molecule expression in alveolar macrophages, limiting CD4⁺ T cell activation

Laurisa M. Ankley, et al., bioRxiv - Immunology 2023

Quote: ... CD4+ T cells were enriched using a MojoSort™ T cell isolation kit (BioLegend, Cat no. 480006) following the manufacturer’s protocol.

Long-term hematopoietic stem cells trigger quiescence in Leishmania parasites

Laura Dirkx, et al., bioRxiv - Microbiology 2023

Quote: ... parasites were stained with 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (CFSE; Cell Division Tracker Kit, BioLegend) according to manufacturer’s instructions ...

Oncostatin M is a Master Regulator of an Inflammatory Network in Dnmt3a-Mutant Hematopoietic Stem Cells

Logan S. Schwartz, et al., bioRxiv - Cell Biology 2023

Quote: ... Cells were stained with Annexin V and Propidium iodide using the Annexin A5 Apoptosis Detection Kit (BioLegend). Data was collected on a FACSymphony A5 (BD Biosciences).

Epigenomic Analyses Identify FOXM1 As a Key Regulator of Anti-Tumor Immune Response in Esophageal Adenocarcinoma

Benjamin Ziman, et al., bioRxiv - Cancer Biology 2023

Quote: ... CD8+ T Cells were isolated using the MojoSort™ Mouse CD8+ T Cell Isolation Kit (Biolegend, #480035) and resuspended in RPMI-1640 medium (Corning ...

Sexual dimorphism in obesity is governed by RELMα regulation of adipose macrophages and eosinophils

Jiang Li, et al., bioRxiv - Immunology 2023

Quote: ... Dead cells were labeled with Zombie Aqua™ Fixable Viability Kit (Cat# 423102 Biolegend, San Diego, CA). Gating strategy was followed ...

Memory Th1 cells modulate heterologous diseases through innate function

NIkolas Rakebrandt, et al., bioRxiv - Immunology 2023

Quote: CD4+ T cells were isolated from the spleen using MojoSort Mouse CD4 T cell isolation kit (BioLegend) and sorted according to their expression of CD44 and NKG2D ...

Ginsentide TP1 Protects Hypoxia-Induced Dysfunction and ER Stress-Linked Apoptosis

Bamaprasad Dutta, et al., bioRxiv - Pharmacology and Toxicology 2023

Quote: Apoptotic cells were detected using an Annexin V Apoptosis Detection Kit with PI (BioLegend Inc., CA, USA). HUVEC-CS cells were cultured in DMEM with or without 20 µM TP1 under normoxic or hypoxic conditions for 24 h ...

Human pluripotent stem cell-derived respiratory airway progenitors generate alveolar epithelial cells and recapitulate features of idiopathic pulmonary fibrosis

Mikael G. Pezet, et al., bioRxiv - Cell Biology 2023

Quote: ... Endoderm yield was determined by dissociating EBs and evaluating CXCR4 and c-KIT (Biolegend, San Diego, CA) co-expression by flow cytometry on day 4 ...

Organelle phenotyping and multi-dimensional microscopy identify C1q as a novel regulator of microglial function

Pooja S. Sakthivel, et al., bioRxiv - Neuroscience 2023

Quote: ... Annexin: Apoptossi was assessed using PE Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend, cat#: 640934). iMGL were collected and washed twice with Biolegend’s cell staining buffer ...

Murine modeling of menstruation identifies immune correlates of protection during Chlamydia muridarum challenge

Laurel A. Lawrence, et al., bioRxiv - Immunology 2024

Quote: Single-cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kits (Biolegend®), followed by cell surface staining and measurements using a BD LSRFortessa™ or LSRII high-parameter cell analyzer ...

A safe insect-based Chikungunya fever vaccine affords rapid and durable protection in cynomolgus macaques

Awadalkareem Adam, et al., bioRxiv - Microbiology 2024

Quote: ... and growth factors measurement using LegendPlex bead-based multiplex technology and NHP inflammation 13-plex kits (BioLegend). Serum samples were processed in duplicate at a 1:4 dilution according to the manufacturer’s instructions ...

B cell-mediated antigen presentation promotes adverse cardiac remodeling in chronic heart failure

Jana P. Lovell, et al., bioRxiv - Immunology 2024

Quote: ... splenic B cells were isolated according to the MojoSort™ Mouse Pan B Cell Isolation Kit (BioLegend) protocol ...

RAMPs regulate signalling bias and internalisation of the GIPR

Matthew Harris, et al., bioRxiv - Pharmacology and Toxicology 2021

Quote: ... before and after 1 hour incubation at room temperature in 50 μL FACS buffer containing allophycocyanin (APC)-conjugated anti-FLAG monoclonal antibody (BioLegend, diluted 1:100 in FACS buffer). For internalisation and recycling experiments ...

BET Inhibition Enhances TNF Mediated Anti-Tumor Immunity

Lisa C. Wellinger, et al., bioRxiv - Cancer Biology 2021

Quote: ... Cells were stained with 1:500 diluted Zombie NIR (BioLegend #423105) for 20 minutes and washed with PBS + 0.5% BSA + 2mM EDTA ...

Sentinel p16^INK4a+ cells in the basement membrane form a reparative niche in the lung

Nabora Reyes de Mochel, et al., bioRxiv - Cell Biology 2020

Quote: ... and anti-CD31 (APC/Fire750; Cat#102528; BioLegend; used 1:200), respectively ...

Glutamate and γ-aminobutyric acid differentially modulate glymphatic clearance of amyloid β through pulsation- and aquaporin-4 dependent mechanisms

Cheng Wu, et al., bioRxiv - Neuroscience 2020

Quote: ... antibodies specific for Aβ 1-42 (Biolegend, 805501, San Diego, USA) and Aβ 1-40 (Biolegend ...

Langerhans cells and cDC1s play redundant roles in mRNA-LNP induced protective anti-influenza and anti-SARS-CoV-2 responses

Sonia Ndeupen, et al., bioRxiv - Immunology 2021

Quote: ... PD-1 (29F.1A12) and Bcl-6 (K112-91) from BioLegend and BD Biosciences ...

Age-dependent regulation of SARS-CoV-2 cell entry genes and cell death programs correlates with COVID-19 disease severity

Zintis Inde, et al., bioRxiv - Cell Biology 2020

Quote: ... and anti-Cytochrome c-AlexaFluor647 (1:2000, clone 6H2.B4, Biolegend) in a saponin-based buffer (final concentration 0.1% saponin ...

Nanoengineered DNA origami with repurposed TOP1 inhibitors targeting myeloid cells for the mitigation of neuroinflammation

Keying Zhu, et al., bioRxiv - Immunology 2021

Quote: ... APC anti-mouse F4/80 (BioLegend, 123116, clone BM8, 1:100), PE/Cyanine7 anti-mouse CD45 (BioLegend ...

Donor Macrophages Modulate Rejection after Heart Transplantation

BJ Kopecky, et al., bioRxiv - Immunology 2021

Quote: ... anti-mouse CD68 1:200 clone FA-11 (Biolegend Cat# 137002), anti-Ki-67 1:200 (Invitrogen Cat# 14-5698-82 ...

Transcriptional landscape of human microglia reveals robust gene expression signatures that implicates age, sex and APOE-related immunometabolic pathway perturbations

Tulsi Patel, et al., bioRxiv - Genetics 2021

Quote: ... Fixed cells were stained with anti-TMEM119 (1:100, Biolegend; 853302) extracellular primary antibody with Goat anti-mouse IgG secondary antibody conjugated to Alexa-488 (1:100 ...

Transcriptional landscape of human microglia reveals robust gene expression signatures that implicates age, sex and APOE-related immunometabolic pathway perturbations

Tulsi Patel, et al., bioRxiv - Genetics 2021

Quote: ... and anti-CD45 Alexa Fluor 647 (1:100, Biolegend; 304056, HI30) antibodies for 30 minutes on ice ...

Mllt11 regulates the migration and neurite outgrowth of cortical projection neurons during development

Danielle Stanton-Turcotte, et al., bioRxiv - Developmental Biology 2021

Quote: ... Blots were probed with rabbit anti-tubulin ß3 (1:1000, BioLegend), mouse anti-acetylated tubulin (1:20,000 ...

Notch Signaling Commits Mesoderm to the Cardiac Lineage

Evan S. Bardot, et al., bioRxiv - Developmental Biology 2020

Quote: ... anti-human CD172a/b (SIRPα/β) (Biolegend; clone SE5A5; 1:200) anti-human CD13 (Biolegend ...

PTEN Inhibition Ameliorates Muscle Degeneration and Improves Muscle Function in a Mouse Model of Duchenne Muscular Dystrophy

Feng Yue, et al., bioRxiv - Developmental Biology 2020

Quote: ... Alexa Fluor 647 anti-mouse CD68 (FA-11, BioLegend, 1:1,000), or anti-mouse MG53 (gift from Jianjie Ma ...

AP-2γ is Required for Maintenance of Pluripotent Mammary Stem Cells

Vivian W. Gu, et al., bioRxiv - Developmental Biology 2020

Quote: ... The following antibodies were used: CD31-Biotin (Biolegend, 102404, 1:250), TER119-Biotin (Biolegend ...

Liver lipophagy ameliorates nonalcoholic steatohepatitis through lysosomal lipid exocytosis

Yoshito Minami, et al., bioRxiv - Cell Biology 2022

Quote: ... washed and incubated with anti-LAMP1 antibodies (Biolegend, 328606, 1:50) for 20min on ice ...

A synthetic bispecific antibody capable of neutralizing SARS-CoV-2 Delta and Omicron

Tom Z. Yuan, et al., bioRxiv - Immunology 2022

Quote: ... 20 μL anti-FLAG PE mAb (1 μg/mL) (Biolegend 637309) was added to the cells and they were incubated for 30 min at 4 °C and 750 RPM on a thermomixer (Eppendorf ...

Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity