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Citations for BioLegend :
5201 - 5250 of 10000+ citations for Mouse Anti Legionella Pneumophila Antibody LN29 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Human cell subpopulations were detected in the bone marrow of transplanted mice using the following antibodies: anti-human CD45-APC (Biolegend, 368512), anti-human CD3-Pacific Blue (Biolegend ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were first blocked with 5% BSA for 30 min and stained with Alexa Fluor® 647 anti-human CD161 antibody (clone HP-3G10; BioLegend) at 10 μg/ml in blocking solution for 60 min ...
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bioRxiv - Immunology 2021Quote: ... The cells were then stained with PE-conjugated anti-Human IgG Fc antibody (clone M1310G05, BioLegend 410720, San Diego, CA, USA) to detect bound anti-CTLA-4 and with fluorescein isothiocyanate (FITC)-conjugated anti-CD27 (clone O323,
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bioRxiv - Genomics 2019Quote: ... Isolated nuclei were labeled with an Alexa Fluor 647 anti-GFP antibody (338006, clone FM264G, 1:25, BioLegend, San Diego, CA) for 30 minutes and 2 μg/ml DAPI immediately prior to sorting ...
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bioRxiv - Molecular Biology 2019Quote: ... and coupled with 10 μg carrier-free anti-epitope tag antibody (c-myc: Santa Cruz #sc-40; FLAG: Sigma #F1804; GluGlu: Biolegend #901801 ...
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bioRxiv - Systems Biology 2019Quote: ... blocked in TBST buffer supplemented with 5% bovine serum albumin (BSA) and incubated overnight with primary anti-XBP1s antibody (Biolegend Poly6195) at 4°C ...
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bioRxiv - Neuroscience 2020Quote: HA-tagged-ribosome immunoprecipitation in the cerebellum of Gfap-RiboTag mice was performed as it was previously described (Ceolin et al., 2017) using anti-HA antibody (5μl/sample; Biolegend; Cat#901502) and magnetic beads (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were selected using hygromycin and the stable population was sorted on our single cell platform for CD19 expression levels by staining transfected cells with AlexaFluor® 647 anti-CD19 antibody (#302222, Biolegend). Cells were sorted for different expression levels of CD19 and clonal cell lines with expression levels comparable to that of standard CD19 positive cell lines such as Raji ...
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bioRxiv - Biophysics 2021Quote: ... The chambers were then incubated with 10 μg/ml purified Anti-HA 11 Epitope tag antibody (BioLegend, San Diego, CA, USA) in BIB at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then incubated with APC-conjugated anti-human HLA antibody (clone W6-32; cat. no. 311410; BioLegend, San Diego, CA). Cells were subsequently analyzed by flow cytometry for GFP cells/DsRed cells (sensitive/resistant ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... CD81 expression was measured 7 days post-transduction by dislodging cells with TrypLE and live cells were stained with APC-conjugated anti-human CD81 antibody (Biolegend 349509). CD81 expression was assessed on a BD LSRII with >90% of Calu3-CRISPRi cells with CD81 knocked down compared to a non-targeting sgRNA control.
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bioRxiv - Cell Biology 2021Quote: ... PMNs and platelets were visualized by applying 20 μl of platelet-specific FITC-labeled X488 antibody (Emfret, Eibelstadt, Germany) and 20 µl of PE-labeled anti-Ly6G (BioLegend #127608) with a catheter ...
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bioRxiv - Microbiology 2021Quote: ... followed by washing and incubated with a solution of AlexaFluor® 647 conjugated anti-His antibody (BioLegend #652513, 10 µg/mL). After washing and drying ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with rat polyclonal T antigen antibody (1:500) followed by an anti-rat secondary (Biolegend, 1:200). For measuring sialic acid binding dependence ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue homogenates were centrifuged to remove tissue debris before being incubated with Anti-HA.11 Epitope Tag Antibody (Biolegend, Cat# 901515) for 4 hours in a microcentrifuge tube on a microtube rotator kept at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... and resuspended in 100 μL of the FCM buffer with 5μL of the secondary Antibody (PE-anti human IgG Fc Recombinant, Biolegend, Catalogue 366903) and incubated for 30 minutes at 4°C. ...
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bioRxiv - Microbiology 2022Quote: Lung tissue leukocyte samples were subjected to viability staining and blocking of Fc-receptors using LIVE/DEAD™ Fixable Blue Dead cell stain kit (life technologies) and an anti-CD16/32 antibody (clone 93, BioLegend). Cells were then washed and incubated with a staining mix containing antibodies against the following murine antigens ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... 5ug/mL) and anti-CD28 (clone 37.51; 2ug/mL) in the presence of IFN-γ neutralizing antibody (XMG1.2, BioLegend, 10ug/mL) and rmIL-4 (R&D ...
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bioRxiv - Immunology 2022Quote: ... 5ug/mL) and anti-CD28 (clone 37.51; 2ug/mL) in the presence of IL-4 neutralizing antibody (11B11, BioLegend, 5ug/mL) for 18-20 hours before the addition of cytokines as follows ...
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bioRxiv - Cancer Biology 2022Quote: The following primary antibodies with indicated dilution in blocking buffer were used: Rabbit anti-Tuj1 (Covance, BioLegend, MRB-435P, 1:1,000), Mouse anti-Tuj1 (Covance ...
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bioRxiv - Microbiology 2021Quote: ... were injected intravenously into the tail vein with 10-15 μg of Alexa Fluor™ 647-coupled anti-CD31 antibody (clone 390, Biolegend) to label blood vessels ...
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bioRxiv - Cancer Biology 2019Quote: ... 3D-O matrices were enzymatically digested with collagenase and PBMCs were isolated and surface-stained with the following antibodies: FITC conjugated anti-CD3 (300406, Biolegend, CA), PE-Cy5 conjugated anti-CD4 (300508 ...
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bioRxiv - Immunology 2022Quote: ... prM-DENV2-treated PBMCs were treated (1h, 37 °C) in the presence or absence of anti-TNF-α antibody (4 µg/mL, BioLegend). The TNF-α depleted supernatants were used to stimulate HUVEC for 6 hours and the expression of activation markers were analyzed by flow cytometry ...
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bioRxiv - Biophysics 2019Quote: ... siRNA mediated knockdown was further confirmed by imaging cells labelled with Alexa Fluor 488 anti-human FcƐRIɑ antibody AER-37 (CRA-1) (BioLegend Products, Singapore).
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bioRxiv - Immunology 2019Quote: ... MR1 mediated activation of MAIT cells was blocked with 2.5 µg/mL anti-MR1 antibody (clone 26.5, BioLegend, San Diego, USA). When assessing cytokine production and cytotoxic granule content by flow cytometry ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested for 5 min at 400g at RT and stained with Pacific Blue™ anti-human Ki-67 Antibody (Biolegend) according to eBioscience(™ ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were centrifuged at 1800 rpm for 5 mins and then incubated in 2 μg/mL solution of anti-CD11b antibody (Biolegend 101257) and anti-GR1 antibody (Biolegend 108422 ...
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bioRxiv - Immunology 2020Quote: ... TnT cells and Jurkat-derived cell lines were stimulated overnight with either 10 μg ml−1 plate-bound anti-human CD3e antibody (clone OKT3, BioLegend, #317326) or 1X eBioscience Cell Stimulation Cocktail (81 nM PMA ...
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bioRxiv - Cancer Biology 2019Quote: ... TDEs in 100 μl final volume of PBS were stained using the PE anti-human CD9 antibody (Clone HI9a) (BioLegend, CA) and incubated for 1 h at 4°C ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: Approximately 1×106 cells were incubated with the following antibodies in order to characterize spleen immune subpopulations: Alexa Fluor® 488-conjugated-anti rat CD3 (IF4, Biolegend), PE Cy5-conjugated-anti rat CD4 (biolegend) ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with either APC-conjugate anti-human CD99 antibody clone 3B2/TA8 (1:20 dilution, BioLegend 341307), PE anti-human CD166 clone 3A6 (1:20 dilution ...
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bioRxiv - Immunology 2021Quote: ... Any contaminating myeloid cells were removed from cultures prior to experimental setup by negative selection using a biotinylated anti-CD11b antibody (Biolegend, #101204) followed by subsequent Streptavidin MicroBeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... The frozen tissue sections were treated with blocking solution (5% normal donkey serum in PBS) and then stained with FITC-conjugated anti-Ly6G antibody (BioLegend, 1A8). Nuclei were stained by DAPI ...
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bioRxiv - Immunology 2021Quote: ... and a cocktail of fluorescent antibodies: 1 µL each of anti-human CD3 (Brilliant Violet 421-conjugated, Biolegend 344834, clone SK7), anti-human CD69 (PerCP-eFluor710-conjugated ...
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bioRxiv - Neuroscience 2022Quote: ... Multiplexing was performed by washing and hashing nuclei from each sample with one of six unique TotalSeq™-A anti-nuclear pore complex antibodies (BioLegend). Two multiplexed sequencing batches were then sequenced by the Oxford Genomics Centre (Wellcome Centre for Human Genetics ...
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bioRxiv - Cell Biology 2022Quote: Cell suspensions from thymus were incubated with the biotin-conjugated anti-lineage marker antibodies described above together with CD4-APC (Biolegend 100411), CD8b-APC/Cy7 (Biolegend 126620) ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then stained with a PE-labeled anti-huCD4 antibody (SK3 clone, Cat#344606, BioLegend, San Diego, CA, USA) and incubated in the dark for 45 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with PBS supplemented with 2% FBS (PBS+) and then stained with 50µl of an antibody cocktail containing 1:50 diluted anti-human CD45-AF700 (BioLegend, clone HI30), 1:50 diluted anti-human CD3-BV605 (BioLegend ...
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bioRxiv - Genomics 2022Quote: ... Cell pellets were resuspended in FACS buffer (PBS with 1% FBS and 1% penicillin-streptomycin) then stained with the following antibodies: PE anti-CD45 (BioLegend #103105); APC anti-CD31 (Invitrogen #17-0311-80) ...
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bioRxiv - Cancer Biology 2022Quote: ... spun at 700 g for 3 minutes at 4°C and washed in MACS Buffer 3× before being incubated with the following antibodies: APC-conjugated rat anti-CD3 (5 μL/10×106 cells; Biolegend, 100408), PE-conjugated rat anti-CD8b (2.5 μL/10×106 cells ...
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bioRxiv - Bioengineering 2024Quote: ... were coated with 100 µL of 4 µg/mL polyclonal anti-lysostaphin antibody (AICBIOTECH-PAb102) in 1×ELISA coating buffer (BIOLEGEND-421701). Wells were washed with 0.05% Tween 20 (SIGMA-P9416-50ML ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in 3% BSA/ 5% normal goat serum in PBS for 1h and incubated overnight at 4°C with primary antibodies (β-III tubulin/anti-tuj-1, 1/1000, Biolegend n°801202 ...
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bioRxiv - Immunology 2024Quote: The following antibodies were used: unconjugated anti-CD16/CD32 (1 μg per 1×106 cells; clone 93; BioLegend Cat. No. 101320), H2-Dd-FITC (1:200 ...
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bioRxiv - Immunology 2024Quote: ... followed by 5 µL/well of the antibody cocktail for extracellular staining: anti-CD3-BV421 (clone 17A2) (BioLegend, SanDiego, CA, USA) (1:200) ...
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bioRxiv - Immunology 2024Quote: ... the enriched epithelial cell suspension was stained for flow cytometry cell sorting with the following antibodies and dyes: anti-EpCAM-APC-Cy7 clone G8.8 (BioLegend, cat. 118218), anti-CD45-APC clone 30-F11 (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed in 1X PBS and stained with Alexa Fluor 700 anti-human B2M antibody (BioLegend, 395708; 1:20 dilution) for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with standard antibodies for immune and vascular lineage exclusion: anti-CD45-PE/Cy7 (BioLegend, 304016, 1:200), anti-CD31-PE/Cy7 (BioLegend ...
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bioRxiv - Genetics 2023Quote: ... and 72 h by immunofluorescent staining with a PE-conjugated anti-human CD41/CD61 primary antibody (BioLegend, San Diego, CA, USA) using flow cytometric analysis.
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with Alexa Fluor® 488-labeled anti-human CD69 antibody or an isotype control (1 µg/ml, BioLegend) for 45 min ...