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Citations for BioLegend :
5151 - 5200 of 8274 citations for C Reactive Protein CRP ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... or biotinylated anti-repebody antibody (1 μg/mL, AbClon, Seoul, Korea) and HRP-conjugated streptavidin (1:1000 dilution, BioLegend, San Diego, CA, USA). For trastuzumab (Herceptin) ...
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bioRxiv - Immunology 2021Quote: ... Bacteria were then resuspended in 0.2µm-filtered solutions of appropriate secondary reagents (Alexa 647-anti-human IgG, Jackson Immunoresearch 1:200, Brilliant Violet 421-anti-Rabbit IgG, Biolegend 1:200). This was incubated for 10-60 min before cells were washed as above and resuspended for acquisition on a BD LSRII or Beckman Coulter Cytoflex S ...
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bioRxiv - Immunology 2021Quote: ... followed by a single wash and a secondary staining with anti-mFc IgG1: PE and/or APC [clone RMG1-1] at 1:100 (Biolegend, Cat: 406608/406610). After enrichment for S1 ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated at 4°C overnight with rabbit sera against MmuPV1 L1 (a kind gift form Chris Buck, NIH; 1:2500) and chicken anti-keratin 14 (BioLegend 905301; 1:500) diluted in 5% goat serum in PBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were suspended in 300 μL staining buffer and incubated on ice for 30min with FVD780(eBioscience,1:1000) and then with CD45(Biolegend,202205, 1:300) for 30min ...
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bioRxiv - Immunology 2021Quote: ... Cell sorts were performed with MojoSort Mouse Pan B Isolation Kit II (BioLegend, San Diego, CA) for CD19+ cells and with Miltenyi’s magnetic-activated cell sorting (MACS ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD14+ magnetic bead positive selection using the MojoSort Human CD14 Selection Kit (BioLegend). Purified CD14+ monocytes were cultured in complete RPMI supplemented with 100ng/mL each of recombinant human IL-4 and GM-CSF (PeproTech ...
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bioRxiv - Immunology 2021Quote: ... For the exclusion of dead cells Zombie Aqua fixable viability kit (BioLegend, San Diego, CA, USA) was adjoined and incubated for 30 min dRT ...
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bioRxiv - Immunology 2021Quote: ... and for exclusion of dead cells Zombie Aqua fixable viability kit (BioLegend, San Diego, CA, USA) were used ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in FACS buffer and stained using a Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... cells were purified using negative magnetic bead selection (MojoSort Mouse Pan Dendritic Cell Isolation Kit, BioLegend). 2.5×106 BMDCs per well were cultured for 6h in 6-well plates in RPMI complete (R10 ...
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bioRxiv - Cancer Biology 2022Quote: ... MCT-treated CD11b+ cells were isolated from tumors using MojoSort Mouse CD11b Selection Kit (BioLegend, 480109). For transfecting every million primary CD11b+ cells ...
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bioRxiv - Molecular Biology 2022Quote: ... Apoptosis and necrosis were determined by using the FITC Annexin V Apoptosis Detection Kit (BioLegend 640914) and SYTOX™ Red Dead Cell Stain (ThermoFisher S34859) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... splenocytes were stained for 30 min at RT with Zombie Green™ Fixable Viability Kit (BioLegend) and were blocked with anti-mouse CD16/32 (BioLegend) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... cells were stained for 30 min at RT with Zombie Green™ Fixable Viability Kit (BioLegend) and were blocked with anti-mouse CD16/32 (BioLegend ...
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bioRxiv - Microbiology 2022Quote: Serum IFN-γ was assessed using a custom-made mouse panel 13-plex kit (LEGENDplex, BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... the labeled cells were washed and incubated with Zombie UV™ Fixable Viability Kit (BioLegend #423107) in FACS buffer before analysis ...
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bioRxiv - Cell Biology 2019Quote: Apoptosis in mononuclear cell suspensions was assessed using the Annexin V-FITC Apoptosis Kit (BioLegend, 640914) according to manufacturer’s instructions and analyzed via flow cytometry ...
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bioRxiv - Immunology 2019Quote: ... FSC-A scatter plots) and dead cells identified by using Zombie Aqua Fixable Viability Kit (BioLegend) were excluded from analyses ...
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bioRxiv - Immunology 2021Quote: ... with all incubations carried out in selection buffer using the MojoSort Mouse Ly6G Selection Kit (Biolegend), the EasySep Biotin Positive Selection Kit II (Stemcell Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD14+ magnetic bead positive selection using the MojoSort Human CD14 Selection Kit (BioLegend). Purified CD14+ monocytes were cultured in complete RPMI supplemented with 100ng/mL each of recombinant human IL-4 and GM-CSF (PeproTech ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested and immediately processed with the Biolegend Phase-Flow Alexa Fluor 647 kit (BioLegend). Cells were counterstained with DAPI and at least 10 000 events were acquired on a BD Fortessa (Becton Dickinson).
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bioRxiv - Cancer Biology 2021Quote: ... Proliferation of CD8+ T cells was determined using the CFSE Cell Division Tracker Kit (Biolegend 423801) according to the manufacturer’s protocols.
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bioRxiv - Cell Biology 2020Quote: Cell death was detected with the FITC Annexin V Apoptosis Detection kit (BioLegend, Cat No. 640922). Infections were performed as described above with mCherry-Stm at an MOI of 100 ...
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bioRxiv - Immunology 2021Quote: ... with half undergoing naïve CD4+ T cells isolation using MoJo magnetic bead negative selection kits (BioLegend), and the other half undergoing CD90+ cell depletion (BioLegend ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were assessed using the APC AnnexinV Apoptosis Detection Kit with PI (BioLegend, CA, USA; #640932) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... T cells were labeled with 5 µM carboxyfluorescein diacetate succinimidyl (CFSE Cell Division Tracker Kit, Biolegend). For Treg induction and T cell activation ...
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bioRxiv - Cell Biology 2024Quote: ... The remainder of the cells were labelled using the Zombie Aqua Fixable Viability Kit (BioLegend #423101) according to the manufacturer’s instructions to indicate the viability status of each cell ...
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bioRxiv - Immunology 2024Quote: ... using the MojoSort magnet with MojoSort Mouse CD4 Naïve T Cell Isolation Kit (Cat.#480040 BioLegend). T cell stimulation was induced with anti-CD3ε and anti-CD28 beads (T Cell Activation/Expansion Kit mouse ...
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bioRxiv - Cancer Biology 2024Quote: Annexin V / PI stainings were performed using the APC/FITC Annexin V Apoptosis Detection Kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were enriched from total splenocytes using the EasySep Mouse CD8+ T cell Isolation Kit (Biolegend) at day 3.5 post infection ...
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bioRxiv - Immunology 2023Quote: ... Airway cytokines and chemokines were quantified by multiplex analyses of BALF samples using LEGENDplexTM kits (BioLegend). Quantification of airway bioactive type I/III IFN was conducted by treating type I/III IFN-sensitive cells isolated from Mx2-Luc reporter mice for 24 h with diluted BALF ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained for 30 min at RT with Zombie Green™ Fixable Viability Kit (Biolegend) and were blocked with anti-mouse CD16/32 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... 24h and 48h using an apoptosis detection kit with PE-Annexin V and 7-AAD (Biolegend), according to the manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... Sorted γδ T cells were stained with 2.5 nM CFSE Cell Division Tracker Kit (423801, BioLegend) for 20 min at 37 °C immediately after sorting ...
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bioRxiv - Immunology 2023Quote: Cytokines were measured in serum using the Mouse Inflammation Panel LEGENDplex cytometric bead array kit (Biolegend). Data were analysed in FlowJo and analyte concentrations were determined using a sigmoidal 4PL standard curve in Prism (GraphPad).
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bioRxiv - Neuroscience 2024Quote: ... CD4+ cells were isolated from the spleen using a CD4 isolation kit (BioLegend # 480033; ∼94% purity).
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bioRxiv - Cell Biology 2024Quote: ... The remainder of the cells were stained using the Zombie Aqua Fixable Viability Kit (BioLegend #423101) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were stained with stained for viability with Zombie AquaTM Fixable Viability Kit (BioLegend catalog #423102) at 1:1000 in DPBS for 30 minutes at room temperature following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stained with viability dye Zombie NIRtrade Fixable Viability Kit (Biolegend Inc, catalog number 423105). Stained cells were fixed using eBioscience Fixation/Permeabilization Concentration (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ OTII cells were isolated using MojoSort™ mouse CD4 T Cell Isolation Kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Surface marker staining was performed for 30 min on ice using CD34-eFluor 660 (eBioscience, clone RAM34, 1:100) and ITGA6-pacific blue (Biolegend, clone GoH3, 1:200). Cells were washed with PBS and stained with annexin V-AF488 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were washed with FACS buffer and surface marker staining was performed for 30 min on ice using CD34-eFluor 660 (eBioscience, clone RAM34, 1:100) and ITGA6-PE/Cy7 (Biolegend, clone GoH3, 1:1000). The cells were washed with 1 % BSA in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... followed by the addition of 1:20 (1:40 final) primary antibodies against LAMP1 (AF488- or BV605-conjugated; BioLegend cat. no. 328610 and 328634), and/or TFRC (FITC-conjugated ...
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bioRxiv - Microbiology 2021Quote: ... transferred to a nitro-cellulose membrane and probed with polyclonal rabbit anti CupA (1:1000) and monoclonal mouse anti-RNA polymerase (1:3000, Biolegend, San 397 Diego, CA). Western blots were then probed with IRDye-conjugated anti-mouse and anti-rabbit secondary antibodies (both at 1:15,000 ...
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bioRxiv - Immunology 2022Quote: ... table 1) were diluted in blocking solution (F4/80 [Bio-Rad]: 10 µg/ml, Ly6G [abcam]: 1 µg/ml, MHC-II [BioLegend]: 5 µg/ml), CD163 [Invitrogen] ...
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bioRxiv - Cell Biology 2020Quote: ... Surface marker staining was performed for 30 min on ice with the following antibodies: CD34-eFluor 660 (eBioscience, clone RAM34, 1:100) and ITGA6-PE/Cy7 (Biolegend, clone GoH3, 1:1000) for sorting or CD34-eFluor 660 (eBioscience ...
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bioRxiv - Neuroscience 2022Quote: ... APC-Cy7 rat anti-mCD45 (1:200, Becton Dickinson, Franklin Lakes, NJ, USA) and APC-Cy7 anti-mTer119 (1:100, Biolegend, San Diego, CA, USA). In order to sort neural stem and precursor cells ...
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bioRxiv - Developmental Biology 2023Quote: ... and subsequently segregated into hormone-sensing and alveolar through the staining with Brilliant Violet 605™ Sca-1 antibody (Biolegend #108133, dilution 1:40) and PE CD49b antibody (Biolegend #108907 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and subsequent 1:200 APC-labeled anti-HER2 antibody (Biolegend) incubation ...