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Citations for BioLegend :
5101 - 5150 of 5248 citations for Mouse MEP1B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Both FLT3L and GM-CSF BMDCs were stained with 1:1000 Zombie Green Fixable Viability Kit (BioLegend, Cat# 423111) and were blocked with anti-mouse CD16/32 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... The percentage of apoptotic cells were then determined using the FITC Annexin V apoptosis detection kit with PI (BioLegend) and flow cytometry as per manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from naïve gender- and age-matched WT and CD6Δd3 splenocytes by negative selection using magnetic beads (Mojo sort kit, BioLegend). Isolated cells were activated with 3 μg plate-bound anti-mouse CD3 (145-2C11 ...
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bioRxiv - Cancer Biology 2022Quote: ... and lineage-negative cells (Lin-) were analyzed for c-Kit (clone 2B8; eBioscience) and Sca-1 (clone D7; BioLegend) expression to discriminate Kit+ Sca-1- (KL ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were prepared for flow cytometry two days after media change using the FITC Annexin V kit (Biolegend, 640906). Quantification was performed using a BD FACSCanto 10 flow cytometer.
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bioRxiv - Neuroscience 2020Quote: ... cells were harvested and stained with AV and PI according to manufacturer’s manual (FITC Annexin V Apoptosis Detection Kit with PI; Biolegend). Stained cells were immediately analyzed by flow cytometry (MACSQuant ...
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bioRxiv - Cancer Biology 2021Quote: ... Apoptosis was determined by detecting phosphatidylserine using the APC-Annexin-V Apoptosis Detection Kit with PI (Biolegend, CA, USA). Briefly cells were harvested ...
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bioRxiv - Cancer Biology 2021Quote: Trypsinized and washed Panc02 cells were stained with the FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend) and analyzed using the CytoflexS Flow Cytometer (Beckman Coulter).
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized with a Transcription Factor Staining Kit (eBioscience) and stained using anti-IFNγ (XMG1.2; 200x; BioLegend) and recombinant mouse IL21R Fc Chimera (600x ...
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bioRxiv - Immunology 2021Quote: CD4+ T-cells were isolated from PBMC using the MojoSort™ Human CD4 T Cell Isolation Kit (480009; BioLegend) or by flow-cytometric sorting ...
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bioRxiv - Immunology 2021Quote: For differentiation of live and dead cells we stained cells with the Zombie Violet Fixable Viability Kit according to the manufacturer’s instructions (BioLegend). For surface marker analysis ...
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bioRxiv - Immunology 2022Quote: ... cells were washed again using ice-cold PBS and stained using Zombie Aqua™ Fixable Viability Kit (Biolegend; 423101) for 20 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: Cytokine concentration of the basolateral compartments were measured using the human antivirus response panel (13-plex) kit (LEGENDplex BioLegend). The kit was used according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2022Quote: ... Apoptotic cells were detected using the PE Annexin V Apoptosis Detection Kit with 7-AAD according to the manufacturer’s protocol (BioLegend) and analyzed using an LSRII flow cytometer.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and labeled with propidium iodide (PI) and Annexin-V-FITC (Annexin V FITC Apoptosis Detection Kit with PI, Biolegend) for the detection of necrosis and apoptosis ...
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bioRxiv - Immunology 2024Quote: ... coupled with the protocol TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 (Single Index) from BioLegend.
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in 50 µl of the Annexin V Apoptosis Detection Kit/7-AAD solution (BioLegend) and incubated for 15 min ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were first stained with viability stain (Zombie NIR fixable viability kit, BioLegend, San Diego, California, USA) followed by either BV510-αCD45 (clone ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated from the spleen using Mojosort Magnetic Separation System CD8 Naïve T cell Isolation Kit (BioLegend). Anti-CD3ε Ab (clone 145-2111C ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was harvested and cytokines were quantified using a Legendplex Mix and Match Kit according to manufacturer instructions (Biolegend).
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bioRxiv - Bioengineering 2023Quote: Lung single-cell suspensions were stained for 30 min at RT with Zombie Red™ Fixable Viability Kit (BioLegend) and were blocked with anti-mouse CD16/32 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... tissues were blocked for endogenous avidin and biotin using an avidin biotin blocking kit according to the manufacturer’s instructions (BioLegend). Sections were then incubated with biotin conjugated goat anti-chicken (Vector Labs ...
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bioRxiv - Neuroscience 2023Quote: ... Single cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kit (BioLegend; San Diego, CA) for 20 min at room temperature in phosphate buffer solution pH 7.2 (PBS ...
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bioRxiv - Immunology 2023Quote: Appropriate ELISA kits were used to quantify human or murine IL-6 and/or TNF concentrations according to manufacturer’s recommendations (Biolegend). The 450 nm absorbance was measured by a SpectraMax® plate reader.
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were purified from spleens and lymph nodes using MojoSort™ CD4 isolation kit and magnet (Biolegend). Purities of cells were determined for each experiment using flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... resuspended in 1 ml of PBS containing 1 µl live/dead stain (Zombie UV Fixable Viability kit (Biolegend, # 423107)) and incubated on ice for 30 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were stained for surface markers and then prepared for intracellular staining using True-Nuclear Transcription Factor staining kits (Biolegend). For experiments demonstrating stimulation response ...
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bioRxiv - Genetics 2021Quote: ... we negatively isolated memory T cells with a modified Pan T cell magnetic-activated cell sorting (MACSR) kit with anti-CD45RA biotin and followed an optimized version of the CITE-seq protocol with TotalSeqTM-A (BioLegend) oligonucleotide-labeled antibodies for a panel of 31 surface proteins (43) ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining for Foxp3 was performed on single cell suspensions from spleen and LNs specifically using a FOXP3 Fix/Perm Kit (BioLegend).
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bioRxiv - Molecular Biology 2020Quote: ... and c-Kit on mast cells were analyzed by Flow cytometry using fluorochrome labelled antibodies (PE-CY7–conjugated anti-FcεRIα, MAR-1 Biolegend #134317; allophycocyanin-CY7–conjugated anti–c-Kit, 2B8, Biolegend, #105825). The YFP+ cells were FACS-sorted and deletion of the Gata2 gene was determined by PCR to be greater than 99%.
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bioRxiv - Molecular Biology 2020Quote: ... CD3 T cells were separated from freshly PBMCs by using a MojoSort™ Human CD3+ T cell Isolation Kit (Biolegend, https://www.biolegend.com/protocols/mojosort-isolation-kits-protocol-1/4599/).
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bioRxiv - Immunology 2019Quote: ... This pooled sample of ∼300 million cells was then evenly distributed across each of the 372 wells of a LEGENDScreen kit (BioLegend) containing reconstituted PE antibodies (Supplementary Table 2) ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...
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bioRxiv - Immunology 2021Quote: Paired maternal and infant plasma were thawed and tested in the 13-plex LegendPlex Human Anti-Virus Response Panel kit (Biolegend), to quantify levels of IL-1β ...
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T lymphocytes were purified (> 98%) by negative selection with MojoSort™ Human CD3+ T cell Isolation Kit (Biolegend, USA). Cells were seeded in CD3-coated plates (clone OKT3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed twice using ice-cold PBS and stained using Zombie Aqua(tm) Fixable Viability Kit (Cat. 423101, Biolegend). Cells were washed and resuspended in FACS buffer (1% BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... FOXP3 APC (Clone FJK-16s eBioscience) used after cell permeabilization with the kit Perm Buffer (10X) and Fix/Perm Buffer (4X) (BioLegend), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were blocked/permeabilized using 10% normal goat serum in 0.3% Triton-X in PBS for 3 hours at room temperature followed by additional avidin biotin blocking using a kit according to manufacturer’s instructions (BioLegend, 927301). Primary immuno-staining was performed using antibodies ...
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bioRxiv - Biochemistry 2022Quote: To monitor the cytotoxicity of MbxA the release of cytosolic LDH was measured as an indicator of membrane damage using the LDH Cytox Assay Kit (Biolegend).
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bioRxiv - Physiology 2022Quote: ... Samples were incubated with anti-c-kit rat monoclonal antibody (1:500 v/v, ACK2, BioLegend, San Diego, CA, USA) overnight (4ºC) ...
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bioRxiv - Cancer Biology 2022Quote: ... and analyzed for apoptosis/necrosis using the APC Annexin V Apoptosis Detection Kit with Propidium Iodide (BioLegend, San Diego, CA). Cells were analyzed using a BD Symphony Flow Cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Cell-free BAL fluid was stored at 4°C and processed within 24hr to measure cytokine/chemokine content using a LEGENDplex bead array kit (BioLegend).
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bioRxiv - Immunology 2020Quote: ... The cells were stained extracellularly to detect ILC surface markers and intracellularly for IFNγ expression using the intranuclear staining kit (Biolegend). Sorted ILC1-like and NK cells were stimulated with human (h ...
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bioRxiv - Immunology 2022Quote: ... 5×104 Tconv (CD4+CD25-) cells from TFAM control were labeled with CFSE Cell Division Tracker Kit (Cat# 423801, BioLegend) and cultured with mitomycin-treated wild type splenocytes ...
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bioRxiv - Neuroscience 2022Quote: ... CD4+ T cells were isolated from the spleens and draining lymph nodes of 2D2 mice using the CD4+ isolation kit (BioLegend) and co-cultured with irradiated wild-type splenocytes at a ratio of 1:5 ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were analyzed by using either the Legendplex Multi-Analyte Flow Assay Kit for human CD8/NK cells or the human Th cytokine panel (Biolegend).
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bioRxiv - Cancer Biology 2019Quote: ... approximately 1 * 106 cells from dLN and 5 × 106 tumor cells were first stained with Zombie Red Fixable Viability Kit (BioLegend) for 30’ at room temperature and then incubated on ice for 15’ with an anti-mouse CD16/32 antibody (BioLegend) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were either stained with DAPI for viability or fixed with 1% PFA and stained with zombie aqua fixable viability kit (Biolegend). Viable cells were sorted on a FACSAria flow cytometer (Becton Dickinson) ...
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bioRxiv - Microbiology 2020Quote: To assess cell viability human neutrophils at indicated times were washed with PBS and incubated with Zombie Aqua Fixable Viability Kit (BioLegend) for 15 min at RT ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...