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• Translatome analysis reveals microglia and astrocytes to be distinct regulators of inflammation in the hyperacute and acute phases after stroke

  Victoria G. Hernandez, et al., bioRxiv - Neuroscience 2023

  Quote: ... CD11b (1:200, BioLegend, 101216), HA (1:200 ...
• Divergent phenotypes in germline versus conditional mutant mouse models of Sifrim-Hitz-Weiss Syndrome

  Sarah Larrigan, Shrilaxmi Joshi, Pierre Mattar, bioRxiv - Neuroscience 2023

  Quote: ... rat anti-Chd4 (1:100, BioLegend 942302), rat anti-Ctip2 (1:200 ...
• Translatome analysis reveals microglia and astrocytes to be distinct regulators of inflammation in the hyperacute and acute phases after stroke

  Victoria G. Hernandez, et al., bioRxiv - Neuroscience 2023

  Quote: ... The following antibodies were used for surface receptor labeling: CD45 (1:200, BioLegend, 103112), CD11b (1:200 ...
• Translatome analysis reveals microglia and astrocytes to be distinct regulators of inflammation in the hyperacute and acute phases after stroke

  Victoria G. Hernandez, et al., bioRxiv - Neuroscience 2023

  Quote: ... biotinylated anti-HA (mouse, 1:500, BioLegend 901505), anti-CD68 (rat ...
• Translatome analysis reveals microglia and astrocytes to be distinct regulators of inflammation in the hyperacute and acute phases after stroke

  Victoria G. Hernandez, et al., bioRxiv - Neuroscience 2023

  Quote: ... and Cx3cr1 (1:200, BioLegend, 149009). Cells were washed in buffer and analyzed with an LSR II cytometer (BD Biosciences) ...
• Olfactory and neuropeptide inputs to appetite neurons in the arcuate nucleus

  Donghui Kuang, et al., bioRxiv - Neuroscience 2023

  Quote: ... and biotinylated anti-HA antibodies (BioLegend, #901505, 1:300) at 4°C for overnight or at 37°C for an hour ...
• Zika virus infection induces IL-1β-mediated inflammatory responses by macrophages in the brain of an adult mouse model

  Gi Uk Jeong, et al., bioRxiv - Microbiology 2023

  Quote: ... Brilliant Violet 421 anti-mouse/human CD11b Antibody (101236, BioLegend, San Diego, CA, USA), PE/Cyanine7 anti-mouse CD45 Antibody (103114 ...
• ATG5 selectively engages virus-tethered BST2/Tetherin in an LC3C-associated pathway

  Delphine Judith, et al., bioRxiv - Microbiology 2023

  Quote: ... or control isotype (Biolegend). The cells were washed three times in cold PBS/1% (w/v ...
• IL-13 promotes sensory-sympathetic neurons crosstalk in asthma

  Théo Crosson, et al., bioRxiv - Neuroscience 2023

  Quote: ... 1×103 counting beads (Biolegend, #424902) were added for absolute quantification before data acquisition on a FACS Canto II (BD Biosciences) ...
• Prdm16 and Vcam1 regulate the postnatal disappearance of embryonic radial glia and the ending of cortical neurogenesis

  Jiwen Li, et al., bioRxiv - Neuroscience 2023

  Quote: ... GFAP (Biolegend, 829401 ...
• In Vivo Biocompatibility of ZIF-8 for Slow Release via Intranasal Administration

  Sneha Kumari, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... Deep blue cell viability kit was purchased from Biolegend. 0.3% carbon monoxide + 0.3% neon + 21% oxygen + balance nitrogen gas mix cylinder was purchased from SCI Analytical Laboratories ...
• Length-dependent translation efficiency of ER-destined proteins

  Hana Sahinbegovic, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Primary antibodies used at indicated dilutions include anti-GFP (MMS-118P, Biolegend, 1:2000), anti-RPS10 (sc-515655 ...
• Community composition and the environment modulate the population dynamics of type VI secretion in human gut bacteria

  Sophie Robitaille, et al., bioRxiv - Microbiology 2023

  Quote: ... Secondary antibodies donkey anti-rabbit IgG HPR conjugated (Biolegend, San Diego, CA) was used with a 1:7500 dilution ...
• HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral Keratinocytes

  William T Seaman, et al., bioRxiv - Microbiology 2023

  Quote: ... anti-loricrin (PRB-145P; Biolegend), anti-GAPDH (sc-25778 ...
• The Cytomegalovirus M35 Protein Modulates Transcription of Ifnb1 and Other IRF3-Driven Genes by Direct Promoter Binding

  Hella Schwanke, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Murine anti-V5-tag (clone 7/4, #680602) and rabbit anti-V5-tag (Polyclonal, #903801) antibodies were purchased from BioLegend (San Diego, CA, USA). Anti-V5-tag mAb-magnetic beads (#M167-11 ...
• Cytotoxic CNS-associated T cells drive axon degeneration by targeting perturbed myelinating oligodendrocytes in PLP1 mutant mice

  Tassnim Abdelwahab, et al., bioRxiv - Neuroscience 2023

  Quote: ... mouse anti-SMI32 (1:1,000, catalog no. 801701; BioLegend), rat anti-MHC-I (1:100 ...
• Cytotoxic CNS-associated T cells drive axon degeneration by targeting perturbed myelinating oligodendrocytes in PLP1 mutant mice

  Tassnim Abdelwahab, et al., bioRxiv - Neuroscience 2023

  Quote: ... rat anti-CD45 PE (1:100, catalog no. 103105; BioLegend). Cells were washed twice ...
• Cytotoxic CNS-associated T cells drive axon degeneration by targeting perturbed myelinating oligodendrocytes in PLP1 mutant mice

  Tassnim Abdelwahab, et al., bioRxiv - Neuroscience 2023

  Quote: ... armenian hamster anti-CD103 BV605 (1:100, catalog no. 121433; BioLegend), rat anti-Ly6A/E FITC (1:100 ...
• Cytotoxic CNS-associated T cells drive axon degeneration by targeting perturbed myelinating oligodendrocytes in PLP1 mutant mice

  Tassnim Abdelwahab, et al., bioRxiv - Neuroscience 2023

  Quote: ... and rat anti-Ly6A/E FITC (1:100, catalog no. 108105; BioLegend). Cells were washed twice ...
• Cytotoxic CNS-associated T cells drive axon degeneration by targeting perturbed myelinating oligodendrocytes in PLP1 mutant mice

  Tassnim Abdelwahab, et al., bioRxiv - Neuroscience 2023

  Quote: ... rat anti-CD8 PerCP/Cyanine5.5 (1:100, catalog no. 100733; BioLegend), rat anti-CXCR6 PE/Cyanine7 (1:100 ...
• A shift in the mechanisms controlling hippocampal engram formation during brain maturation

  Adam I. Ramsaran, et al., bioRxiv - Neuroscience 2023

  Quote: ... Membranes were incubated in primary antibodies diluted in 5% skim milk in TBST: mouse anti-brevican (1:1000, BioLegend, cat# 820101), sheep anti-neurocan (1:1000 ...
• Cytotoxic CNS-associated T cells drive axon degeneration by targeting perturbed myelinating oligodendrocytes in PLP1 mutant mice

  Tassnim Abdelwahab, et al., bioRxiv - Neuroscience 2023

  Quote: ... rat anti-CXCR4 PE (1:100, catalog no. 146505; BioLegend), armenian hamster anti-CD103 BV605 (1:100 ...
• Cytotoxic CNS-associated T cells drive axon degeneration by targeting perturbed myelinating oligodendrocytes in PLP1 mutant mice

  Tassnim Abdelwahab, et al., bioRxiv - Neuroscience 2023

  Quote: ... rat anti-CXCR4 PE (1:100, catalog no. 146505; BioLegend), armenian hamster anti-CD103 BV605 (1:100 ...
• Cytotoxic CNS-associated T cells drive axon degeneration by targeting perturbed myelinating oligodendrocytes in PLP1 mutant mice

  Tassnim Abdelwahab, et al., bioRxiv - Neuroscience 2023

  Quote: ... rat anti-CXCR6 PE/Cyanine7 (1:100, catalog no. 151118; BioLegend), rat anti-CXCR4 PE (1:100 ...
• Cytotoxic CNS-associated T cells drive axon degeneration by targeting perturbed myelinating oligodendrocytes in PLP1 mutant mice

  Tassnim Abdelwahab, et al., bioRxiv - Neuroscience 2023

  Quote: ... armenian hamster anti-CD103 BV605 (1:100, catalog no. 121433; BioLegend), and rat anti-Ly6A/E FITC (1:100 ...
• Cytotoxic CNS-associated T cells drive axon degeneration by targeting perturbed myelinating oligodendrocytes in PLP1 mutant mice

  Tassnim Abdelwahab, et al., bioRxiv - Neuroscience 2023

  Quote: ... rat anti-Ly6A/E FITC (1:100, catalog no. 108105; BioLegend), rat anti-CD11b (1:100 ...
• Cytotoxic CNS-associated T cells drive axon degeneration by targeting perturbed myelinating oligodendrocytes in PLP1 mutant mice

  Tassnim Abdelwahab, et al., bioRxiv - Neuroscience 2023

  Quote: ... For further experiments viable CD45highCD8+ cells were labeled with rat anti-CD45 APC (1:100, catalog no. 103111; BioLegend), rat anti-CD8 PerCP/Cyanine5.5 (1:100 ...
• Hepatocyte-specific miR-33 deletion attenuates NAFLD-NASH-HCC progression

  Pablo Fernández-Tussy, et al., bioRxiv - Physiology 2023

  Quote: ... BV711 CD4 - 100447 (Biolegend), BV605 CD8a - 100744 (Biolegend ...
• Hepatocyte-specific miR-33 deletion attenuates NAFLD-NASH-HCC progression

  Pablo Fernández-Tussy, et al., bioRxiv - Physiology 2023

  Quote: ... BV605 CD8a - 100744 (Biolegend) and activation was determined according to CD62L/CD44 status with PE-Cy7 CD62L - 25-0621-82 (eBioscience ...
• Osteoblast-intrinsic defect in glucose metabolism impairs bone formation in type II diabetic mice

  Fangfang Song, et al., bioRxiv - Physiology 2023

  Quote: ... CD3-PE (Biolegend, Ref#100206, clone 17A2), B220-PE (Biolegend ...
• Osteoblast-intrinsic defect in glucose metabolism impairs bone formation in type II diabetic mice

  Fangfang Song, et al., bioRxiv - Physiology 2023

  Quote: ... CD19-PE (Biolegend, Ref#115508, clone 6D5), Gr-1-PE (Biolegend ...
• Osteoblast-intrinsic defect in glucose metabolism impairs bone formation in type II diabetic mice

  Fangfang Song, et al., bioRxiv - Physiology 2023

  Quote: ... Cd11b-PE (Biolegend, Ref#101208, clone M1/70) and Calcein AM (Thermo Fisher Scientific ...
• Osteoblast-intrinsic defect in glucose metabolism impairs bone formation in type II diabetic mice

  Fangfang Song, et al., bioRxiv - Physiology 2023

  Quote: ... CD71-PECy7 (Biolegend, Ref#113812, clone RI7217), CD45-PE (eBioscience ...
• Osteoblast-intrinsic defect in glucose metabolism impairs bone formation in type II diabetic mice

  Fangfang Song, et al., bioRxiv - Physiology 2023

  Quote: ... Gr-1-PE (Biolegend, Ref#108408, clone RB6-8C5), Cd11b-PE (Biolegend ...
• Osteoblast-intrinsic defect in glucose metabolism impairs bone formation in type II diabetic mice

  Fangfang Song, et al., bioRxiv - Physiology 2023

  Quote: ... B220-PE (Biolegend, Ref#103208, clone RA3-6B2), CD19-PE (Biolegend ...
• Hepatocyte-specific miR-33 deletion attenuates NAFLD-NASH-HCC progression

  Pablo Fernández-Tussy, et al., bioRxiv - Physiology 2023

  Quote: ... B cells were identified as APC-Cy7 B220 (Biolegend, USA); T cells were identified as CD4hi or CD8hi with the following antibodies ...
• The Small GTPase Rab7 Regulates Release of Mitochondria in Extracellular Vesicles in Response to Lysosomal Dysfunction

  Wenjing Liang, et al., bioRxiv - Physiology 2023

  Quote: ... incubated with antibodies against CD45 (BioLegend, clone I3/2.3), CD11b (Tonbo Bioscience ...
• The Small GTPase Rab7 Regulates Release of Mitochondria in Extracellular Vesicles in Response to Lysosomal Dysfunction

  Wenjing Liang, et al., bioRxiv - Physiology 2023

  Quote: ... and the cell pellet resuspended in staining buffer (BioLegend). Cells were seeded in 96-well plate ...
• GPR41/43 regulates blood pressure by improving gut epithelial barrier integrity to prevent TLR4 activation and renal inflammation

  Rikeish R. Muralitharan, et al., bioRxiv - Physiology 2023

  Quote: Concentrations of tumour necrosis factor (TNF)-α from tissue culture supernatants post- stimulation were measured using ELISA MAX Standard Set Mouse TNF-α kit (Biolegend) according to the manufacturer’s protocol.
• GPR41/43 regulates blood pressure by improving gut epithelial barrier integrity to prevent TLR4 activation and renal inflammation

  Rikeish R. Muralitharan, et al., bioRxiv - Physiology 2023

  Quote: ... and stained with anti-CD45 and anti-F4/80 (rat anti-mouse, Biolegend) and then fixed and permeabilised using the FOXp3/Transcription Factor Staining Buffer Set (eBioscience) ...
• Neurological Disorder after Severe Pneumonia is Associated with Translocation of Bacteria from Lung to Brain

  Qingle Ma, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-CD14-APC (BioLegend). The stained cells were analyzed using BD Accuri C6 flow cytometer and analyzed using Flowjo_V10 software based on 100,000 gated events.
• Killing them softly with EPO: a new role for erythropoietin in the homeostasis of red blood cells

  Clemente F. Arias, et al., bioRxiv - Physiology 2023

  Quote: ... and stained with allophycocyanin (APC) conjugated streptavidin (Biolegend), which binds the biotin.
• Killing them softly with EPO: a new role for erythropoietin in the homeostasis of red blood cells

  Clemente F. Arias, et al., bioRxiv - Physiology 2023

  Quote: ... We analyzed the expression of CD47 (phycoerythrin-conjugated anti-mouse CD47 antibody, Biolegend) in reticulocytes (CD71+ ...
• Killing them softly with EPO: a new role for erythropoietin in the homeostasis of red blood cells

  Clemente F. Arias, et al., bioRxiv - Physiology 2023

  Quote: ... the cells were stained with F4/80 (using FITC-conjugated anti-mouse antibody, Biolegend) and SIRP-α (PE-conjugated anti-mouse antibody ...
• Multiscale networks in multiple sclerosis

  Keith E. Kennedy, et al., bioRxiv - Systems Biology 2023

  Quote: ... BV421-conjugated anti-CD25 (Clone BC96, catalog # 302630, BioLegend), AF700-conjugated anti-CD127 (Clone A019D5 ...
• Multiscale networks in multiple sclerosis

  Keith E. Kennedy, et al., bioRxiv - Systems Biology 2023

  Quote: ... APC Cy7-conjugated anti-CD4 (Clone SK3, catalog #344616, BioLegend), BV421-conjugated anti-CD25 (Clone BC96 ...
• A systematic search for RNA structural switches across the human transcriptome

  Matvei Khoroshkin, et al., bioRxiv - Systems Biology 2023

  Quote: ... and anti-IFNγ (BioLegend 502520). Samples were analyzed on BD LSRFortessa cell analyzer.
• Multiscale networks in multiple sclerosis

  Keith E. Kennedy, et al., bioRxiv - Systems Biology 2023

  Quote: ... PE-conjugated anti-CD24 (Clone ML5, catalog # 311105, BioLegend) and PE/Dazzle594-conjugated anti-CD38 (Clone HB-7 ...
• Multiscale networks in multiple sclerosis

  Keith E. Kennedy, et al., bioRxiv - Systems Biology 2023

  Quote: ... PBMC in triplicate tubes were stained with BV510-conjugated anti-CD3 (Clone OKT3, Catalog # 317332, BioLegend)) ...
• Multiscale networks in multiple sclerosis

  Keith E. Kennedy, et al., bioRxiv - Systems Biology 2023

  Quote: ... APC-conjugated goat anti-mouse IgG (Catalog # 405308, BioLegend; 1:100 dilution), or APC-conjugated goat anti-rat IgG (Catalog # 405407 ...