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Citations for BioLegend :
5001 - 5050 of 10000+ citations for Mouse Vasoactive Intestinal Polypeptide Receptor 1 VIPR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... anti-Galectin-3 (1:50; R&D systems, AF1197 & 1:100; Biolegend, 125401), anti-Hsp70 (1:300 ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell, cat. no. 17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend, cat. no. 480082) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were resuspended in FACS buffer (PBS, 1% FBS, 1 mM EDTA, 0.02% NaN3) and treated with anti-CD16/CD32 (clone 93, Biolegend 101302, 1:100) for 20 min on ice before staining.
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bioRxiv - Immunology 2024Quote: ... CD4-PE (in-house, GK1.5, 1:400), CD8α-A594 (in-house, 53-6.7, 1:400) and CD3χ-PE/Cy7 (BioLegend, 145-2C11, 1:1000) as well as CD45.2 in either BV421 (TR-A ...
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bioRxiv - Immunology 2024Quote: ... CD4-A594 (in-house, GK1.5, 1:400), CD8α-A647 (in-house, 53-6.7, 1:400) and CD3χ-PE/Cy7 (BioLegend, 145-2C11, 1:1000) and scored as CD3χ + cells ...
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bioRxiv - Cancer Biology 2024Quote: Mice with a human engraftment above 5% measured through flow cytometry using mCD45 (eFluor 450, eBiosciences, 1:100), hCD7 (PE, eBiosciences, 1:25) and hCD45 (Alexa Fluor 647, BioLegend, 1:25) were started on treatment which consisted of 0.50 mg kg−1 of vincristine (1 mg ml−1 injection ...
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bioRxiv - Immunology 2021Quote: ... total thymocytes were processed to single cell suspension in 10% FBS/HBSS (20×106 cells/ml) and incubated with rat anti-mouse CD24 antibodies (M1/69, Biolegend) (30 μg/100 ×106 cells ...
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bioRxiv - Immunology 2021Quote: ... Antibodies for flow cytometry and cell isolation against the following proteins (all mouse unless otherwise indicated) were obtained from BioLegend, eBioscience ...
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bioRxiv - Immunology 2021Quote: Cytokine levels in supernatants from stimulated cells were determined using the LEGENDplex™ Mouse anti-viral response panel (Biolegend, USA) as per manufacturer’s instructions and read on BD Accuri™ C6 Plus Flow Cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... total lung tissue was marked with a lineage antibody (Lin, anti-mouse CD3ε, Ly-6G / Ly-6C, CD11b, CD45R / B220, TER-119 PE, Biolegend), anti-EpCAM for 10 minutes at temperature environment ...
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bioRxiv - Immunology 2022Quote: ... total lung tissue was marked with a lineage antibody (Lin, anti-mouse CD3ε, Ly-6G / Ly-6C, CD11b, CD45R / B220, TER-119 PE, Biolegend), anti-CD45 (BD Bioscience ...
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bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
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bioRxiv - Immunology 2021Quote: FACS purified cell subsets were labeled by incubation for 30 minutes at 4°C with TotalSeq anti-mouse Hashtag antibodies (BioLegend), then washed ...
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bioRxiv - Immunology 2021Quote: ... TCR expression was analyzed the next day by flow cytometry using an anti-mouse TCR antibody (H57-597, BV421, BioLegend).
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bioRxiv - Immunology 2020Quote: Cytokine concentrations in supernatants of in vitro cultures were analyzed by the LegendPlex Mouse Th Cytokine Panel (13-plex) (BioLegend) according to the manufacturer’s instructions and analyzed on a FACS LSR II (BD Biosciences).
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bioRxiv - Immunology 2019Quote: Splenic TFH and TFR cell were stained with antibodies against mouse CD4 (Clone: RM4-5, Biolegend, San Diego, CA, USA), CD44 (Clone:IM7 ...
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bioRxiv - Immunology 2019Quote: ... mice were injected intraperitoneally with 100μg anti-TNFα (Ultra-LEAF™ Purified anti-mouse TNFα Antibody, clone MP6-XT22 Biolegend) or 200μg intraperitoneally (ip ...
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bioRxiv - Immunology 2019Quote: The following antibodies were used for extracellular flow cytometry: anti-mouse CD45 BV650 (clone 30-F11, Biolegend, San Diego, CA), anti-mouse/human CD11b APC-Cy7 (clone M1/70 ...
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bioRxiv - Immunology 2021Quote: Mouse cytokines and chemokines were quantitated in graft and spleen tissue using the LEGENDplex Mouse Inflammation and custom plex panel (Biolegend) according to manufacturer’s instruction 22 ...
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bioRxiv - Neuroscience 2021Quote: The following antibodies were used at the indicated concentrations (IHC-immunohistochemistry; ICC-immunocytochemistry; IB-immunoblot): purified anti-HA mouse (Biolegend Cat# 901501 ...
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bioRxiv - Cancer Biology 2020Quote: ... This was followed by a 10 min incubation with anti-mouse Fc block and a 30 minute cell surface staining with anti-mouse CD4-APC/Cy7 and CD8-PE/Cy7 (Both from Biolegend). CFSE labeled CD8+ CTLs were subsequently sorted on a BD FACS Aria III (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... Platelet activation was detected by direct immunofluorescence labeling of alpha granule release marker CD62P (P-selectin) with mouse anti-human monoclonal antibody CD62P-AlexaFluor647 (Clone AK4, Cat.No. 304918, BioLegend, USA). and activation associated conformational change in integrin αIIbβ3 was detected with a mouse anti-human monoclonal antibody PAC1-FITC (Clone PAC-1 ...
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bioRxiv - Microbiology 2021Quote: ... and the membranes were probed with a rabbit polyclonal antibody generated against the FHA mature C-terminal domain (MCD)(10) and a mouse monoclonal antibody generated against an HA epitope (BioLegend). Corresponding α-rabbit and α-mouse IRDye secondary antibodies (LI-COR Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed with water three times before applying 25 μL of secondary antibody (goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking solution ...
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bioRxiv - Cancer Biology 2020Quote: ... The homogenates were then resuspended in PBS buffer and stained with anti-mouse CD45.1 antibody conjugated with Brilliant Violet 421 (clone A20; BioLegend, 103134), CD11b monoclonal antibody conjugated with APC (M1/70 ...
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bioRxiv - Cancer Biology 2021Quote: ... Then the tissues were washed multiple times in PBS at room temperature for one day and put into a staining solution containing Alexa Fluor 488 anti-mouse CD41 antibody (clone MWReg30, BioLegend) at a 1:100 dilution and Alexa Fluor 555 donkey anti-goat antibody (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... PE anti-human TRAILR4 (DcR2) (R&DSystems, Clone 104918) or PE Mouse IgG1 κ Isotype Control (Biolegend, Clone MOPC-21) for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: C26 tumor-bearing mice were injected intraperitoneally with 200 μg of GR1-depleting antibody (Ultra-LEAF anti-mouse-Ly6G/Ly6C Ab, BioLegend). Control mice were injected intraperitoneally with 200 μg of isotype control (Ultra-LEAF rat IgG2b ...
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202; CD4, eBioscience, 16-0041-82; CD5, BioLegend, 100602; CD8, BioLegend, 100702; CD11b, BioLegend, 101202 ...
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bioRxiv - Cancer Biology 2022Quote: ... were added to the in vivo samples to obtain absolute counts of events acquired on the cytometer as well as using human and mouse Fc blocking antibodies (Biolegend) to reduce non-specific antibody staining ...
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bioRxiv - Immunology 2022Quote: ... and PE-conjugated anti-mouse CCR5 (a Th1 marker (27)), CCR3 (a Th2 marker (28)), or CCR6 (a Th17 marker (29, 30)) antibodies (BioLegend). Finally ...
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bioRxiv - Bioengineering 2022Quote: ... and Phycoerythrin-conjugated Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody (Fisher) for ACE2-Bite and APC anti-human IgG Fc Antibody clone HP6017 (Biolegend) for ACE2-Fc were added to the wells and incubated for another hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and PE-conjugated anti-mouse CCR5 (a Th1 marker (27)), CCR3 (a Th2 marker (28)), or CCR6 (a Th17 marker (29, 30)) antibodies (BioLegend). For the MHC ...
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bioRxiv - Immunology 2022Quote: ... Cell samples at each timepoint were prepared as described and co-stained with PE-anti-CD19 and allophycocyanin-conjugated mouse anti-human CD23 (APC-anti-CD23, BioLegend, clone EBVCS-5 ...
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bioRxiv - Physiology 2022Quote: ... the cell pellet was resuspended in 100 μl of Alexa Fluor 647-conjugated anti-human/mouse SSEA1 antibody (catalog no. MC-480; BioLegend) diluted 1:100 in PBS + 2.5% FBS and incubated at room temperature for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... and then stained for 30 minutes at 4℃ with the following antibody panel each diluted in PBS: APC/Cyanine7 anti-mouse CD45 antibody (Biolegend) or FITC anti-mouse CD45.2 antibody (Biolegend) ...
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bioRxiv - Cell Biology 2022Quote: ... BSA- and IgG-coated dynabeads for pre-clearing were prepared by mixing dynabeads slurry (40 μl per sample) with 500 μl PBS/3% BSA or 500 μl PBS with 2.5 μg mouse IgG1 (BioLegend, 400102) respectively ...
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bioRxiv - Immunology 2020Quote: Cytokine concentrations in culture supernatant or mouse plasma were measured with the LegendPLEX Inflammation Panel (13-plex) or the Mouse Th1 Panel (5-plex) per the manufacturer protocols (BioLegend). Quantification was done using LegendPLEX software v8.0 for Windows ...
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bioRxiv - Microbiology 2021Quote: ... cell surface markers were detected by a 30 minute-incubation at 4°C with 4 µg/mL Pacific Blue-conjugated mouse anti-CD14 (clone 63D3, Biolegend), a 1:20 dilution of APC-conjugated mouse anti-CD163 (clone REA812 ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with anti-CoV-2 Spike RBD mAb (SARS2-02) (13) followed by APC-conjugated goat anti-mouse IgG antibody (Cat# 405308, BioLegend). For surface detection ...
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bioRxiv - Microbiology 2021Quote: ... and cells were stained for 30 min at 4°C with a combination of the following anti mouse immunophenotyping antibodies: from Biolegend: PacificBlue-conjugated anti-Ly-6C (clone HK1.4 ...
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bioRxiv - Immunology 2022Quote: ... IL-17A, IL-17F, IL-21, IL-22) and mouse Pro-inflammatory Chemokine Panel (CCL20, CXCL1, CCL2, CCL3, CCL4) (Biolegend) using a FACSVerse flow cytometer (BD Bioscience) ...
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bioRxiv - Immunology 2022Quote: ... and then cell surface staining was performed for 30 min at 4°C with fluorochrome-conjugated monoclonal antibodies to mouse antigens purchased from Biolegend: XCR1 ...
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bioRxiv - Immunology 2022Quote: ... BMDCs were pre-incubated for 20 minutes at 37 °C with anti-mouse IFNAR1 (clone MAR1-5A3, 10 μg/mL, Biolegend), anti-mouse IL-12p40 or anti-mouse IL-12p70 Abs or an isotype control before stimulation.
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bioRxiv - Immunology 2022Quote: ... Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend), soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Cell Biology 2019Quote: ... Peripheral blood was surface stained with APC/Cy7 conjugated anti-mouse CD45 antibody (clone 30-F11, Biolegend, San Diego, CA), APC conjugated anti-mouse CD3 antibody (clone 145-2C11 ...
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bioRxiv - Molecular Biology 2019Quote: ... for 3 min and stained using mouse anti-human HER2 antibody conjugated to FITC (20 μg/ml, clone 24D2, BioLegend). Cells were incubated with anti-HER2 antibody for 20 min at 4°C in the dark and washed once with PBS supplemented with 1% FCS prior to the FACS analysis ...
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bioRxiv - Cancer Biology 2019Quote: ... for 30’ at room temperature and then incubated on ice for 15’ with an anti-mouse CD16/32 antibody (BioLegend). Cell surface markers were stained using APC-coupled AH1 tetramers and fluorochrome-conjugated antibodies against CD8a (FITC) ...
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bioRxiv - Immunology 2020Quote: In some cases we injected retro-orbitally with 10 μg of Alexa Fluor 647 anti-mouse CD31 (BioLegend, no. 110724) and 10 μg of Alexa Fluor 647 anti-mouse CD144 (BioLegend ...
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bioRxiv - Genomics 2020Quote: ... Cytokine concentrations in super-natants of in vitro cultures were analyzed by the LegendPlex Mouse Th Cytokine Panel (13-plex) (BioLegend) according to the manufacturer’s instructions and analyzed on a FACS LSR II (BD Biosciences).