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Citations for BioLegend :
451 - 500 of 4473 citations for Recombinant Mouse Pcsk9 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Immunology 2023Quote: The following list of antibodies and their concentration against mouse proteins were employed: αCD45 (1:400; BioLegend Cat# 103147, RRID:AB 2564383), αCD3ε (1:300 ...
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bioRxiv - Cancer Biology 2020Quote: ... We next incubated them with 3.5 µg/ml biotinylated CD81 or CD63 antibody (BioLegend, clone 5A6, and H5C6) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Additionally, biotinylated antibodies were used for the staining ST2 (IL-33R) (Mdb, clone DJ8) and TSLPR (Biolegend, 22H9). For intracellular staining ...
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bioRxiv - Immunology 2019Quote: ... Primary staining was done with an antibody against MR1 (Clone 26.5, gift from Ted Hansen, biotinylated by Biolegend) at 1:100 for 40 min on ice in the presence of 2% human serum ...
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bioRxiv - Immunology 2020Quote: ... the sections stained for CD301b were incubated with 2.5 μg/ml biotinylated CD45R/B220 (clone RA3-6B2, BioLegend), followed by detection with SA-HRP and Alexa Fluor 647 Tyramide ...
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bioRxiv - Immunology 2021Quote: Biotinylated DPB1*04:01-monomers loaded with TFEYVSQPFLMDLE peptide (S167-180) were tetramerised using PE-Streptavidin (Biolegend, 405204). 1.1 volume PE-conjugated streptavidin was added to 1 volume of DPB1*04:01-monomer (1 mg/mL) ...
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bioRxiv - Developmental Biology 2023Quote: ... then for 1 hour at room temperature (RT) with biotinylated secondary antibodies and finally with fluorochrome-labeled (BioLegend) or peroxidase-labeled streptavidin (Beckman Coulter) ...
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bioRxiv - Cancer Biology 2022Quote: ... The “rescue” wells were treated with 0.8 ug/mL recombinant human periostin (BioLegend 770506). After 24 hours ...
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bioRxiv - Biochemistry 2022Quote: ... and cells were cultured overnight with 0.5 μg/mL recombinant IL-2 (Biolegend 589106) in RPMI + 10% heat-inactivated FBS until use.
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bioRxiv - Developmental Biology 2022Quote: ... Plates were coated with recombinant human E-cadherin-Fc chimera (BioLegend #BLG-779904-25ug) to reduce colony formation and thereby limit the potential impact of paracrine and direct cell signaling in regulating gene expression of related cells 50 ...
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bioRxiv - Immunology 2023Quote: ... and 2 ng/ml human interleukin (IL)-2 recombinant (BioLegend, San Diego, CA, 589104).
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bioRxiv - Bioengineering 2023Quote: ... THP-1 cells were supplemented with 100 U/ml recombinant human IFN-γ (BioLegend) for 3 days to induce PD-L1 expression ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with 200 µg/mL recombinant Galectin-1 and Galectin-3 (Biolegend). Galectin binding was competitively inhibited by adding 50 mM lactose during the staining procedure.
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bioRxiv - Cell Biology 2023Quote: MAD-MB-453 cells were treated with recombinant hTRAIL (30 ng/ml, B294007, Biolegend) for 6 h ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were treated with different combinations of 400 ng/mL human recombinant sFasL (Biolegend), 40 μM Fasudil HCl (SellekChem) ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... the remaining volume was subjected to immunoprecipitation on protein A/G magnetic beads coated with 5 µg of mouse anti-HA IgG (Biolegend, clone 16B12) as described in the Magna RIP technical manual ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and a further 1 h incubation with anti-His HRP conjugate (652504; BioLegend, San Diego, CA, USA) diluted (1:20000 ...
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bioRxiv - Bioengineering 2023Quote: The antibodies used for flow cytometry were as follows: anti-His Tag APC (BioLegend, USA, Cat: #362605), anti-human IgG (G18-145 ...
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bioRxiv - Immunology 2022Quote: ... prior to incubation with staining antibodies (PE/Cy7 anti-mouse CD45, BV711 anti-mouse CD3, BV421 anti-mouse CD19, FITC anti-mouse CD31, and APC anti-mouse podoplanin, all BioLegend) and one Hashtag antibody per replicate using BioLegend TotalSeq-B03 (catalog numbers 155831 ...
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bioRxiv - Cell Biology 2021Quote: ... purified mouse anti mouse/human CD207 (Biolegend, 144202), rabbit anti-mouse/human Ki67 (abcam ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal against mouse β-III-tubulin (Biolegend); mouse monoclonal against HA (12CA5 ...
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bioRxiv - Immunology 2022Quote: Mouse cytokine (LEGENDplex™ Mouse Inflammation Panel, Biolegend) and chemokine (LEGENDplex™ Mouse Proinflammatory Chemokine Panel ...
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bioRxiv - Immunology 2023Quote: ... AF700-conjugated mouse anti-mouse CD45.2 (104; BioLegend), PE-Cy5-conjugated mouse anti-mouse CD45.1 (A20 ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 mg of protein G agarose beads were crosslinked to 5 μL of mouse anti-HA antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006) using an effective concentration of 6.5 mg mL-1 DMP ...
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bioRxiv - Immunology 2021Quote: ... PD-1 tetramers were prepared by incubating a 5:1 ratio of biotinylated PD-1 and PE streptavidin (BioLegend) for 10 minutes on ice in the dark ...
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bioRxiv - Immunology 2020Quote: ... Negative control ‘decoy’ tetramers were similarly assembled using biotinylated Nipah virus soluble glycoprotein 65 and streptavidin-PerCP Cy5.5 (BioLegend). PBMC were washed in cold PBS and seeded at 1 × 106 cells/well in 96-well round bottom plates and with combinations of RBD and decoy tetramers by incubation for 30 min on ice ...
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bioRxiv - Cell Biology 2020Quote: ... Stem and progenitor cells were isolated using fluorescently labeled or biotinylated antibodies for the following antigens: cKit (2B8, Biolegend), Sca1 (D7 ...
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bioRxiv - Immunology 2020Quote: ... Negative control ‘decoy’ tetramers were similarly assembled using biotinylated Nipah virus soluble glycoprotein 65 and streptavidin-PerCP Cy5.5 (BioLegend).
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bioRxiv - Immunology 2022Quote: ... enriched single-cell suspensions from tumor lysates were labelled with biotinylated anti-CD11b antibody (BioLegend, San Diego, CA, USA) and separated on MACS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... and biotinylated isotype controls (Rat IgG2a,κ clone RTK2758 and Rat IgG2b,κ clone RTK4530) were purchased from Biolegend. Biotinylated antiPD-1 antibody (clone J43 ...
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bioRxiv - Bioengineering 2023Quote: ... The 5x diluted streptavidin-coated nanovial suspension was then incubated with an equal volume of 71.5 μg/mL of biotinylated anti-human VEGF antibody (Biolegend) for 30 minutes at room temperature on a tube rotator and washed ...
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bioRxiv - Immunology 2023Quote: ... fibroblasts and in vitro differentiated plasmablasts (generally <10% frequency) were removed using biotinylated anti-H-2Kd (Biolegend, cat: 116604) and anti-CD138 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... IFNγ ELISAs used the MD-1 clone for coating and a biotinylated 4S.B3 clone for detection prior to visualization with a streptavidin-HRP antibody (BioLegend).
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bioRxiv - Immunology 2024Quote: ... Truncated EGFR expression was detected using biotinylated cetuximab (Bristol-Myers Squibb) and a phycoerythrin (PE)-conjugated streptavidin antibody (BioLegend). T cell activation was assessed by fluorophore-conjugated monoclonal antibodies detecting CD137 (also known as TNFRSF9 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell sorting for GFP-positive CD298+MHC-I− PDX cells with fluorescently tagged antibodies for CD298 (Biolegend, cat. no. 341704) and MHC-I (eBioscience ...
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bioRxiv - Cell Biology 2022Quote: ... or P14 bone marrow cells from 2-4 mice per genotype were c-kit selected and stained with tagged Total-Seq B antibodies (Biolegend) to CD150 ...
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bioRxiv - Immunology 2022Quote: ... then incubated at 37 °C in the presence or absence of recombinant murine CCL28 (BioLegend) at the indicated concentrations ...
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bioRxiv - Neuroscience 2020Quote: ... neuronal cultures were treated with 200 ng/mL recombinant human NRG1 (BioLegend, Cat. No. 551904) for 48 hrs ...
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bioRxiv - Immunology 2021Quote: ... Sorted transduced macrophages were stimulated with 5 ng/mL recombinant murine IFN-γ (575304, Biolegend) 12-14 hours before stimulation with 10 ng/mL recombinant TNFα as described above for 4 hours (FBS used in these experiments was from Omega ...
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bioRxiv - Immunology 2024Quote: ... The ultrasound-guided intra-amniotic injection of recombinant human HMGB1 (Biolegend, San Diego, CA, USA) at a concentration of 9 ng dissolved in 100 μL of sterile 1X phosphate-buffered saline (PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... one uterine horn of XER mice was injected intraluminally with 1µg recombinant Lif (BioLegend, 554006) at 1300h on GD3 ...
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bioRxiv - Immunology 2023Quote: ... 550 µl of RPMI medium supplemented with 1 µg/ml recombinant human CCL21 (BioLegend; #582208) or nothing as negative control were added to the lower chambers ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed with PBS containing 1% FBS and incubated with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Binding of anti-TIGIT antibodies HB0036/HB0030 to cell surface was detected by APC-anti his (Biolegend/362605). For ELISA based assay of anti-TIGIT antibodies in tumour digest ...
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bioRxiv - Cancer Biology 2019Quote: ... and mouse TruStain FcX (anti-mouse CD16/32, Biolegend) and incubating at 4C for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... and AF700-conjugated mouse anti-mouse CD45.2 (104; BioLegend) along with eF780 viability dye (BioLegend ...
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bioRxiv - Microbiology 2022Quote: ... PE-Cy5-conjugated mouse anti-mouse NK1.1 (PK136; BioLegend), PE-Cy5.5-conjugated rat anti-mouse CD4 (RM4-5 ...