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Citations for BioLegend :
451 - 500 of 8415 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Single cell suspension was obtained and then stained with 1:50 CD31 (BioLegend, 102405), 1:50 CD45 (BioLegend ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies used were: H-2Kb/H-2Db (FITC, Biolegend, #114606, 1:50 dilution), HLA-A,B,C (FITC ...
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bioRxiv - Immunology 2023Quote: ... Alexa Fluor 700 anti-mouse/human CD45R/B220 antibody(1:50; Biolegend, RA3-6B2), BUV395 anti-mouse CD11b(1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... and mouse IgG1-anti-rat MHCII (OX-6, 1:100, # 50-165-477 Biolegend) to label MHCII+ cells.
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bioRxiv - Immunology 2024Quote: ... following Fc receptor saturation (CD16/32, clone 93, dilution 1:50 in PBS, Biolegend) and dead cell detection with Fixable Viability Stain 440UV (dilution 1:500 in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... and for mammary epithelial cells subsets: anti-CD326 (EpCAM)-PE (BioLegend, 324206, 1:50) and anti-CD49f-FITC (BioLegend ...
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bioRxiv - Physiology 2024Quote: ... and then resuspended in 50 μL Zombie Near Infrared (Zombie-NIR; 1:1,000; BioLegend) in DPBS ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 µL of flow buffer containing anti-CD45-BV785 antibody (Biolegend, 103149, 1:3000) was added ...
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Single-cell assessment of trophoblast stem cell-based organoids as human placenta-modeling platformsbioRxiv - Developmental Biology 2022Quote: ... followed by 30 minutes incubation with anti-EGFR BV605 (clone AY13; 1:50; Biolegend) and anti-HLA-G APC (clone MEMG-9 ...
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bioRxiv - Immunology 2023Quote: ... Alexa Fluor 594-conjugated mouse anti-rat IgG2a (1:50, clone MRG2a-83, BioLegend), Alexa Fluor 488-conjugated goat anti-rat IgM (1:50 ...
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bioRxiv - Cancer Biology 2022Quote: ... CD45 was stained using biotin-conjugated anti-CD45 (Biolegend; cat#304004; 1:50 dilution) and visualized by incubation with Alexa555-labeled streptavidin ...
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bioRxiv - Physiology 2024Quote: CD31 staining: Alexa Fluor 647 anti-mouse CD31 antibody (Biolegend, 102515, 1:50 dilution). LMNB1 staining ...
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bioRxiv - Immunology 2024Quote: ... allophycocyanin (APC)/Fire 750 anti-human CD8a (1:50 dilution, BioLegend, clone HIT8a, 300931), phycoerythrin (PE)/Cyanine7 anti-human CD69 (1:50 dilution ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated with 50µL of 1:50 TruStain FcX anti-mouse (BioLegend, 101320) for 10 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... typically using 50 μL of PE anti-mouse H-2Kb (BioLegend, 1 μg/mL) for staining 0.25 million cells on ice for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... then stained in FACS buffer + 1:50 Human TruStainX (Fc receptor block (Biolegend #422302) with antibody or isotype control for 30 minutes at 4C ...
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bioRxiv - Neuroscience 2023Quote: ... 1-16 Antibody (1:000, Clone 6E10, N-terminal, a.a. 1-16, human specific only, 803014 BioLegend, CA, USA); β-Amyloid ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD49a APC (1:50; clone HMα1) and anti-CD11b BV570 (1:100; clone M1/70) from BioLegend. Cells were fixed for 30 min at 4°C using eBioscicencesTM FoxP3/Transcription factor staining buffer set (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... an RBD - HC2.RBD.rFC (Metafora – 1:5) was used in conjunction with Alexa 647 donkey anti rabbit Poly4064 (1:50 Biolegend).
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bioRxiv - Immunology 2024Quote: ... the supernatant was discarded and the cell pellet was resuspended in 50 µL of FACS Buffer (0.5% BSA, 2 mM EDTA in DPBS) with 1 µL of human TruStain Fc-block (1:50, Biolegend) and incubated for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... The percentage of apoptotic cells were then determined using the FITC Annexin V apoptosis detection kit with PI (BioLegend) and flow cytometry as per manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... the cells were harvested and washed twice before subjecting to propidium iodide (PI) solution (10 µg/ml) (BioLegend, #421302) for 10 min ...
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bioRxiv - Immunology 2021Quote: ... Monensin (BD Biosciences, CA; BioLegend, CA) was added to the wells 1 hour after peptide addition to prevent cytokine secretion as recommended by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... We aspirated pelleted barcoded cells to a volume of 50 µL and incubated them with 15 µL of Human TruStain FcX (Biolegend, San Diego, CA) for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... and incubated with 1 µL/mL Brefeldin-A (BioLegend, San Diego, CA) for 4-6 hours at 37°C ...
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bioRxiv - Immunology 2020Quote: ... anti-CD19-PE-Cy7 (Biolegend, San Diego, CA, USA, #115520; 1:100); hamster anti-mouse anti-CD3-FITC (BD Pharmingen™ ...
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bioRxiv - Cell Biology 2021Quote: AlexaFluor 647 mouse-anti-hLAMP1 (1:500, Biolegend, Carlsbad, CA, USA; #328611), AlexaFluor 647 mouse-anti-hLAMP2 (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... AlexaFluor 647 mouse-anti-hLAMP2 (1:500, Biolegend, Carlsbad, CA, USA; #354311), mouse-anti-EEA1 (1:1000 ...
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bioRxiv - Bioengineering 2021Quote: ... PBMCs were labeled with CFSE (Supplemental Table 1, Biolegend, San Diego CA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... cells were also stained with Sca-1 PerCP-Cy5.5 (Biolegend, CA USA). Cells were then fixed (Fixation buffer ...
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bioRxiv - Immunology 2020Quote: ... and PE anti-mouse CD45 (1:400, Cat. 103105, BioLegend CA, USA) and the percentage of CD11b+/CD45+ cells was determined by flow cytometry ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit antibody against Pax6 at 1:100 (AB_2313780, BioLegend, San Diego, CA), mouse antibody against RET-P1 at 1:1000 (anti-Rhodopsin ...
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bioRxiv - Immunology 2022Quote: ... FITC mouse anti-human PD-1 (EH12.2H7) (BioLegend, San Diego, CA, USA), and PE-eFluor 610 mouse anti-human CXCR5 (MU5UBEE ...
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bioRxiv - Immunology 2024Quote: ... and APC anti-mouse P2RY12 (S16007D) (1:100) (BioLegend, San Diego, CA). For TMEM119 staining ...
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bioRxiv - Immunology 2024Quote: ... Ly-6G-647 antibody (1:200, #127610, RRID:AB_1134159, BioLegend, San Diego, CA) and DAPI (1:500 of 10 mg/ml stock ...
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bioRxiv - Cell Biology 2024Quote: ... and THY-1(CD90)-APC (clone 5E10; BioLegend, San Diego, CA, USA). Propidium iodide (PI ...
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bioRxiv - Immunology 2021Quote: ... 1:400), CD4-Spark685 (SK3, 1:200), streptavidin-APC-Fire750, CD3-APC-Fire810 (SK7, 1:50) and Zombie NIR (all BioLegend) diluted in Brilliant Staining buffer (BD Horizon) ...
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bioRxiv - Immunology 2021Quote: ... 1:400), CD4-Spark685 (SK3, 1:200), streptavidin-APC-Fire750, CD3-APC-Fire810 (SK7, 1:50) and Zombie NIR (all BioLegend) diluted in Brilliant Staining buffer (BD Horizon) ...
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bioRxiv - Cell Biology 2020Quote: ... All FACS antibodies were used at 1:100 dilution in the presence of TruStain fcX blocker (101320, Biolegend, 1:50). For PDGFRa immunofluorescence staining ...
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bioRxiv - Immunology 2020Quote: ... at a total concentration of 5×106 cells/mL in RPMI 1640 + 10% FBS + 1% Pen/Strep +50U/mL IL-2 (R10-50) with 1/100 anti-CD107a-PE antibody (Biolegend) and 1/1,000 Monensin GolgiStop™ (BD) ...
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bioRxiv - Immunology 2023Quote: ... and stained for 30 min at 25 °C with BCL6-PE (7D1, 1:50) and Ki-67-PE-Cy7 (Ki-67, 1:400) (both BioLegend). Cells were washed twice with permeabilization/wash buffer and resuspended in P2 for acquisition on an Aurora using SpectroFlo v2.2 (Cytek) ...
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bioRxiv - Immunology 2023Quote: ... for 5 min on ice before staining with fluorochrome-conjugated antibodies in FACS staining buffer (1× PBS, 2% FBS): CD3 (1:50 dilution; clone 145-2C11, BioLegend), CD8α (1:200 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed once with PBS and incubated in 50 µL PBS containing human Fc block (Supplementary Table 1) and Zombie Aqua viability dye (1:200, BioLegend) for 15 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... CD40-APC (1:100) and CD11c-e450 (1:100) (all antibodies from Biolegend, San Diego, CA, USA). Cells were incubated with 100μl of the antibody suspension for 20 minutes at 4°C before a final wash in Ca2+/Mg2+ free PBS containing 2mM EDTA at x400g for 5 minutes at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... 0,05% in PBS) and stained with antibodies: anti-EpCAM (APC-Cy7-conjugated, Biolegend,1:50), CD49F (APC-conjugated ...
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bioRxiv - Cell Biology 2021Quote: ... grids were then incubated in a 1:50 dilution of mAb414 (BioLegend, San Diego, USA) for two hours ...
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bioRxiv - Cancer Biology 2021Quote: ... and stained with anti-Foxp3 AF488 (clone MF-14, BioLegend, lot# B248077, 1:50 dilution) or rat IgG2bϰ AF488 (clone RTK4530 ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated with a CD16/CD32 receptor blocking antibody (1:50, BioLegend, 101302, clone 93) for 10 min ...
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bioRxiv - Immunology 2020Quote: ... The second antibody master mix used included 1:50 of anti-CD3 BV650 (OKT3, BioLegend), 1:50 of anti-CD4 BV711 (OKT4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Paraffin-embedded tissue sections were stained with βIII-tubulin antibody (1:50; Biolegend, cat. 801202) using a mouse-on-mouse kit (Vector Laboratories ...