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Citations for BioLegend :
451 - 500 of 1373 citations for L Phenylalanine N T Boc 2 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were enriched from mouse spleen and lymph nodes by negative selection using MojoSort Mouse CD4 Naive T Cell Isolation Kit (MojoSort, Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... we obtained splenocytes from WT and TCF-7 cKO mice and stimulated T cells with 2.5ug/ml anti-CD3 (Biolegend #100202) and anti-CD28 antibodies (Biolegend #102115 ...
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bioRxiv - Immunology 2022Quote: ... samples were stained with the following antibodies in 1X eBiosciences permeabilization buffer for 30 minutes at room temperature: T-bet (1:100; clone 4B10; BioLegend); Foxp3 (1:300 ...
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bioRxiv - Immunology 2020Quote: The cytokines IFNγ and TNFα contained in supernatants derived from 1-4 h BxPC3 - CAR T cell co-cultures were quantified by ELISA using Abs from Biolegend.
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bioRxiv - Immunology 2020Quote: ... The plates were washed four times with PBS-T and developed with 100 µL/well TMB High Sensitivity substrate solution (BioLegend, UK). After 5 to 10 min the reaction was stopped with 100 µL 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek) ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated with K562 at a 1:1 E/T ratio with addition of a CD107a FITC mAb (H4A3, BioLegend) in a 96-round bottom plate with a centrifugation step of 5min at 500rpm ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: Levels of IFN-γ in supernatants from T cell clone recognition assays were measured by ELISA using the ELISA MAX Deluxe Set Human IFN-g kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... or polarized to Th1 phenotype by culturing in T cell media additionally supplemented with IL-12 (20 ng/mL) and anti-IL-4 (10 µg/ml) (Biolegend). After 48 h ...
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bioRxiv - Immunology 2022Quote: ... LN and thymic homogenates were stained with a cocktail of antibodies against CD4-FITC and CD25-APC (for LN Tregulatory cells; Mouse Regulatory T Cell Staining Kit #1, eBioscience) or CD4-PB (Biolegend), CD8-FITC ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Neuroscience 2022Quote: ... CD4+ T cells were isolated from the spleens and draining lymph nodes of 2D2 mice using the CD4+ isolation kit (BioLegend) and co-cultured with irradiated wild-type splenocytes at a ratio of 1:5 ...
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bioRxiv - Immunology 2019Quote: ... Naive CD4+ and CD8+ T cells were isolated using a negative selection cocktail containing the following biotinylated mouse antibodies (Biolegend): TCR γ/δ (clone GL3) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C in primary antibodies diluted in 1-5% serum in PBS-T according to the following concentrations: novel anti-parkin mAbs from Biolegend clones D (BioLegend ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD4 or CD8 T cell magnetic bead negative selection using the MojoSort Human CD4 or CD8 Selection Kit (BioLegend). Isolated CD4 or CD8 T cells were labeled with CellTrace Violet (CTV ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (1% BSA in PBS) at a concentration of 108 cells/mL and 1 μL negative CD8+ T cell biotin-antibody cocktail (Biolegend)/million cells was added and allowed to incubate for 15 min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Activated CD4+ and CD8+ T cells were stimulated with a cell activation cocktail (Biolegend; CA PMA/Ionomycin with Brefeldin A) overnight and stained for IFN-γ and TNF-α after fixation and permeabilization using the eBioscience/Thermo kit (Grand Island ...
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bioRxiv - Cancer Biology 2022Quote: ... is the complete list of antibodies used for experiments of T cell activation or exhaustion against primary AML: Anti-CD279 (PD-1) FITC (cat#329904 from Biolegend), CD366 (TIM-3 ...
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bioRxiv - Immunology 2022Quote: ... The 1G4 murine CD8+ T cells were additionally stained with: mouse-anti-human TCR Vβ 13.1-PE (clone: H131, Biolegend, 362409). For in vivo studies ...
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bioRxiv - Microbiology 2022Quote: ... the BrdU-labeled splenocytes were surface stained for T-cell (CD3e-APC; eBioscience) marker after blocking with anti-mouse CD16/32 antibodies (BioLegend). Cells were then permeabilized and treated with DNase to expose BrdU epitopes followed by anti-BrdU-FITC and 7-AAD (7-amino-actinomycin D ...
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bioRxiv - Immunology 2022Quote: ... The membrane was washed three times with TBS-T for 10 min and subjected to the HRP-conjugated secondary goat anti-mouse antibody (Biolegend) in a solution of 3% non-fat milk in TBS-T for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Following combinations of cytokines and antibodies were used for the differentiation of T cells: for Th1 cells - 1 µg/ml anti-IL-4 (clone 11B11; Biolegend), 30 U/ml rhIL-2 (NIH) ...
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bioRxiv - Immunology 2023Quote: ... CAR-T cells were cocultured with NALM6GL cells at indicate E/T ratio for 4 hours with the presence of 1x Brefeldin A (Biolegend). Cells were first stained with the Live/Dead-NearIR dye before fixed ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokine stain was performed following restimulation of pmel-1 T cells as above with the addition of 1X Brefeldin A (Biolegend) and 1X Monensin (Biolegend ...
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bioRxiv - Immunology 2022Quote: T cells were adjusted at a concentration of 1 × 106 cells/100 μl CTL medium including Golgi-Stop (Biolegend, 420701) and Golgi-Plug (Biolegend ...
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bioRxiv - Bioengineering 2023Quote: ... Binding of T cell-secreted SiRPα-Fc on T cell CD47 was assessed by staining with labeled goat anti-human IgG Fc (HP6017, Biolegend) at 4°C for 30min ...
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bioRxiv - Immunology 2023Quote: ... were isolated from single cell suspension of lymph nodes and spleen by negative selection using the MojoSort Mouse CD8 T cell isolation kit (BioLegend). Alternatively ...
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bioRxiv - Immunology 2023Quote: ... CD4 and CD8β cells were depleted from the T-IEL compartment using CD4 and CD8β biotinylated antibodies and magnetic streptavidin nanobeads (MojoSort) (Biolegend). CD8α+ cells were purified from CD4 and CD8β depleted T-IEL and lymph node T cells using the CD8α Positive Selection Kit (Stemcell Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... Pospho-GSK-3α/β and pospho-Akt were quantitated following 5 minutes of stimulation in transfected T cells fixed and permeabilized with fixation and permeabilization buffers (Biolegend) and stained with anti-pospho-GSK-3α/β (R&D systems ...
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bioRxiv - Cancer Biology 2023Quote: ... The frequency of CD8+ CD57+ population in freshly isolated T cells was assessed by using PE-conjugated anti-CD8 (clone RPA-T8, Biolegend) and PerCP-conjugated anti-CD57 (clone HNK- ...
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bioRxiv - Cancer Biology 2023Quote: ... PBMCs and HeLa target cells were co-cultured in u-bottom 96 well plates at a range of T:E ratios (indicated in figure legends) T cells were stimulated with 10 µg/mL plate-immobilised anti-CD3 (OKT3, BioLegend) and cultured for 4 days prior to staining with anti-CD8-APC (SK1 ...
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bioRxiv - Bioengineering 2023Quote: ... whereas binding of T cell-secreted A4 monomer on OT-I T cell CD47 was assessed indirectly by staining with anti-mouse CD47 (miap301, Biolegend) at 4°C for 30min.
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bioRxiv - Immunology 2023Quote: Cytokines secreted in the culture supernatant of cytokine-stimulated sip-T were measured by a LEGENDplex Human CD8/NK Panel kit (BioLegend) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11, BioLegend) and 1 μg mL−1 soluble anti-CD28 (eBioscience ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Cancer Biology 2023Quote: ... T cells were first processed for surface marker labeling by incubation with anti-CD8-BV421 and anti-CD4-BV650 (Biolegend) for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were enriched from the spleen of OT- I mice using the MojosortTM Mouse CD8+ T cell isolation kit (Biolegend) and stained with 5µM CellTrace Violet (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: All flow cytometry was performed at Fred Hutchison Cancer Center utilizing a 28-color panel focused on T cell phenotyping (Supplementary Table 1) [33] CVC and PBMC were incubated with Fc-blocking reagent (BioLegend) and fixable UV Blue Live/Dead reagent (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... reactivity of TCR-transduced PBMC-derived CD8+ T-cells was evaluated by flow analysis following anti-CD107a (BioLegend, Cat #328620) and anti-CD137 antibody (4-1BB ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected at d5 of the culture for the analysis of T cell cytokine production using a combination of commercially available antibodies (Biolegend). Briefly ...
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bioRxiv - Immunology 2023Quote: ... peptide-specific T cells were evaluated by FCM by staining with PE-conjugated MART-1(A27L) tetramer and anti-CD8 FITC (BioLegend) (Supplementary Table 1).
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bioRxiv - Immunology 2024Quote: ... Naive CD8+ T cells were isolated by negative selection using the MojoSort Mouse CD8+ Naïve T cell Isolation Kit as per manufacturer’s instructions (BioLegend, >90% purity). Live cells were counted by trypan blue dye exclusion and resuspended at a concentration of 106 cells/mL in complete T cell media (RPMI-1640 (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T-cells were enriched from the spleen of OT-II mice using the MojoSort mouse CD4+ T-cell isolation kit (BioLegend) and labelled with CellTrace Violet (5 μM ...
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bioRxiv - Immunology 2024Quote: ... Mice CD4+ T cells (4x105/well in 96-well plate) were activated with plate-coated αCD3 (5 µg/ml, Biolegend) and αCD28 (10 µg/ml ...
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bioRxiv - Bioengineering 2024Quote: T-cells were cultured in RPMI-1640 media supplemented with 5 µg/mL brefeldin A (BioLegend, San Diego, CA, USA). After 24 hours of cultivation ...
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bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... 1-16 Antibody (1:000, Clone 6E10, N-terminal, a.a. 1-16, human specific only, 803014 BioLegend, CA, USA); β-Amyloid ...
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bioRxiv - Neuroscience 2023Quote: ... 17-24 Antibody (1:1000, Clone 4G8, N-terminal, a.a. 17-24, human & mouse specific, 800708 BioLegend, CA, USA); Amyloid Precursor Protein (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... both endometriosis and sham mice were treated with intraperitoneal (i.p.) injections of 1μg of rmIL-23 (n=6/group; 589006, BioLegend, USA), or PBS for controls (n=6/group) ...
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bioRxiv - Immunology 2024Quote: ... intestine and skin (n = 3, PCW 9.5) were stained with the following antibodies (clone, fluorophore, company): CD3 (UCHT1, FITC, Biolegend), CD7 (M-T701 ...