Labshake search
Citations for BioLegend :
451 - 500 of 7705 citations for 1 Hydroxymethyl benzo cd indol 2 1H one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... for the clone that binds to the high affinity IL-2 receptor (CD25): we incubated 0.5ugr mouse Il-2 (PepreoTech) to 5ugr of an IL-2 mAb (JES6-1A12, Biolegend) for 30 minutes on ice ...
-
bioRxiv - Immunology 2024Quote: ... The amount of IL-2 in the culture medium was measured using a mouse IL-2 ELISA kit (Biolegend).
-
bioRxiv - Bioengineering 2024Quote: ... APC- CD81 (clone Eat-2; 2 µg/mL) detection antibodies or isotypic controls (all antibodies were purchased from BioLegend). Flow cytometry was performed on a MACSQuant 10 instrument (Miltenyi Biotech ...
-
bioRxiv - Immunology 2020Quote: ... We divided the unstimulated cells per donor in half and stained one tube of unstimulated cells and one tube of stimulated cells per donor with an intracellular antibody master mix consisting of anti-IL17A – APC (BioLegend, Clone BL168) and PE conjugated to either anti-IL-4 ...
-
bioRxiv - Immunology 2021Quote: ... Individually titrated working solutions containing one of eight hashtag antibodies were added to each of the eight respective patient samples (Biolegend; Table S13) and incubated on ice for 30 minutes followed by three magnet-based washes in FACS buffer ...
-
bioRxiv - Genomics 2020Quote: ... cells were resuspended in PBS complemented with 2% fetal bovine serum and stained with FITC anti-human CD45 antibody (BioLegend #304006CD45, 1:200 dilution) and PE anti-human EPCAM antibody (Miltenyi Biotech #130-113-264 ...
-
bioRxiv - Immunology 2023Quote: ... TCR-transduced CD8+ T cells were rapidly expanded for 5 days using irradiated feeder PBMCs and medium containing 60U/ml IL-2 (Proleukin, Novartis) and Ultra-LEAF™ Purified anti-human CD3 Antibody (clone OKT3, BioLegend 317326, 1:2000). After 5 days ...
-
bioRxiv - Immunology 2023Quote: ... pDCs were gated for flow cytometry with 1:20 dilution commercial mouse anti-human BDCA-2-Alexa488 (stock 200 ug/mL, BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL ...
-
bioRxiv - Immunology 2021Quote: ... Supernatants were harvested 24 hours later and IL-2 concentrations were determined using serially diluted supernatants and the human IL-2 ELISA MAX Deluxe kit (BioLegend) adapted to 384-well plate format using one quarter of the recommended reaction volumes ...
-
bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
-
bioRxiv - Bioengineering 2021Quote: ... Bcl-2 anti-apoptotic protein expression was measured by intracellular staining of BMMs with PE-Cy7 anti-Bcl-2 antibody (Biolegend). AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend ...
-
bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in media (RPMI1640 + 2% FBS) with recombinant human Nectin-2 (Biolegend) or isotype control (ENZO ...
-
bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
-
bioRxiv - Immunology 2021Quote: ... containing anti-CD9 (human HI9a, mouse MZ3), anti-CD63 (human H5C6, mouse NVG-2), anti-CD81 antibodies (human 5a6, mouse Eat-2) from Biolegend, and anti-human ACE2 (R&D Biosystems cat no ...
-
bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
-
bioRxiv - Cancer Biology 2022Quote: Single-cell suspensions were washed after collection and stained in ice-cold Ca+2/Mg+2-free PBS with a viability dye (Zombie NIR, BioLegend) for 20 min at 4°C in the dark ...
-
bioRxiv - Immunology 2022Quote: IL-2 ELISA was performed using an ELISA MAX™ Deluxe Set Mouse IL-2 as described in manufacturer’s protocol (BioLegend). The O.D ...
-
bioRxiv - Immunology 2023Quote: ... we coated 96 well plates with indicated amounts of anti-CD3ε antibody supplemented with soluble anti-CD28 at 2-times the amount of anti-CD3ε and 5U/mL hL IL-2 (Biolegend). For activation ...
-
bioRxiv - Immunology 2023Quote: ... biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) from BioLegend. SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were then washed twice in FACS buffer (PBS + 2% FBS + 1mM EDTA) before staining with anti-pERK1/2 (Biolegend 369506 100x ...
-
bioRxiv - Immunology 2024Quote: ... human PBMCs were preincubated for 30 min with 2 μg/mL anti-human IL-12 (clone 24910; BioTechne) or 2 μg/mL mouse IgG1 (clone MOPC-21; BioLegend). In functional experiments ...
-
bioRxiv - Immunology 2024Quote: ... cells were supplemented with 25 µl/mL ImmunoCult Human CD3/CD28 T Cell Activator (StemCell) and 20 ng/mL Recombinant Human IL-2 (rhIL-2; Biolegend), seeded in a F-bottom 96-well plate using 4×105 cells (200 µl ...
-
bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 300 g for 5 min at 4°C and cultured in flasks at 106 cells/mL in complete RMPI with 20 ng/mL recombinant mouse IL-2 (rmIL-2; Biolegend). To generate talin-deficient T cells ...
-
bioRxiv - Immunology 2024Quote: ... splenocytes (2*107/mL in IMDM medium containing 2% FCS) were simultaneously stained with anti-B220-Alexa647 (clone RA3-6B2 Biolegend) and loaded with 5 μM Indo-1 acetoxymethyl (Indo1-AM ...
-
bioRxiv - Immunology 2021Quote: ... anti-mouse CD63-160Gd (clone NVG-2, BioLegend), anti-mouse CD120b-166Er (clone TR75-32.4 ...
-
bioRxiv - Immunology 2021Quote: ... Anti-mouse IL-2 (clone JES6-1A12, BioLegend) antibody was used to capture IL-2 from the supernatants ...
-
bioRxiv - Immunology 2020Quote: ... A647 anti-Bcl-2 (633510) (all from BioLegend); APC anti-CXCR4 (558644) ...
-
bioRxiv - Immunology 2022Quote: ... anti-CD138 (BV711, 281-2, AB_2562571, BioLegend #142519), anti-CD19 (BV785 ...
-
bioRxiv - Bioengineering 2021Quote: ... IL-2 PE-Cy7 (Biolegend clone MQ1-17H12), IFNγ APC (Biolegend clone B27) ...
-
bioRxiv - Immunology 2020Quote: ... CD49d (α4-integrin; FITC, Biolegend, Clone: R1-2), CD88 (PE ...
-
bioRxiv - Immunology 2022Quote: ... and 10 ng/mL IL-2 (589102, BioLegend) in RPMI-1640 complete medium for 24 hours before killing to differentiate into T cells ...
-
bioRxiv - Immunology 2022Quote: ... BV785-labeled anti-CD138 (clone: 281-2; BioLegend), APC-labeled anti-IgD (clone ...
-
bioRxiv - Immunology 2022Quote: ... APC-labeled anti-IgD (clone: IA6-2; BioLegend), PerCP-Cyanine5.5-labeled anti-CD38 (clone ...
-
bioRxiv - Immunology 2022Quote: ... and anti-CD32-PE-Cy7 (FUN-2, Biolegend). After ...
-
bioRxiv - Immunology 2020Quote: ... 2 μl of Trustain FcR blocking reagent (BioLegend), 3 μl of anti-human CD45 antibody (clone HI100 ...
-
bioRxiv - Bioengineering 2020Quote: ... Then a biotinylated anti-IL-2 Ab (BioLegend) at a concentration of 0.25 μg/ml was added and after washing ...
-
bioRxiv - Immunology 2022Quote: ... Biotinylated SARS-CoV-2-S1 Protein (Biolegend 793804) Anti-Biotin (409003) ...
-
bioRxiv - Immunology 2020Quote: ... and rIL-2 (10 U/ml; Biolegend, #575402), with or without 2.5 μM mCRAMP ...
-
bioRxiv - Immunology 2020Quote: ... p-Erk1/2 (4B11B69) were purchased from BioLegend. Antibodies against NFAT1 (D43B1) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2 μL anti-Rpb3 antibody (Biolegend, catalog #665003) was conjugated to Dynabeads M-280 sheep anti-mouse IgG beads (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: ... and 20 ng/ml IL-2 (Biolegend; 575406) as previously described (38) ...
-
bioRxiv - Immunology 2021Quote: ... FITC anti-mouse CD14 (clone Sa14-2; Biolegend), rabbit monoclonal Alexa 647 anti-phospho-p65(Ser536 ...
-
bioRxiv - Immunology 2021Quote: ... OX40 PE-Cy7 (2:100; Biolegend Cat#350012), and CD69 PE (10:100 ...
-
bioRxiv - Immunology 2021Quote: ... and anti-IL-2-APC (JES6-5H4, BioLegend) on ice for 1 hr or overnight ...
-
bioRxiv - Immunology 2023Quote: ... and the CD18-reactive mAb M18/2 (BioLegend) were randomly conjugated with Alexa Fluor 647 carboxylic acid succinimidyl ester (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... anti-human CD32 (Biolegend 303205 clone FUN-2), PE anti-human CD64 (Biolegend 305007 clone 10.1) ...
-
bioRxiv - Biophysics 2023Quote: ... and 2 μg/mL recombinant human CCL2 (Biolegend).
-
bioRxiv - Developmental Biology 2023Quote: ... CD63 PE (clone NVG-2, Biolegend, RRID: AB_11203532). DAPI (Millipore ...
-
bioRxiv - Immunology 2022Quote: ... and 2 µg/mL anti-ICOS (C398.4a, BioLegend). Goat anti-hamster IgG (20 µg/mL ...
-
bioRxiv - Immunology 2022Quote: ... and 5 ng/mL IL-2 (Biolegend 575404) at 1×107 cells/mL for 72 hrs ...