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Citations for Tosoh Bioscience :
1 - 50 of 51 citations for Ion Exchange Chromatography since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The nucleosomes were further purified by ion-exchange chromatography (TSKgel DEAE, TOSOH Bioscience, Japan) to remove free DNA and histones ...
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bioRxiv - Biochemistry 2022Quote: ... Annealed DNA duplexes were purified by ion exchange chromatography using a DEAE column (Tosoh Bioscience) under a gradient of 0 to 1 M NaCl in TE Buffer ...
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bioRxiv - Biochemistry 2021Quote: ... further purification by the ion-exchange chromatography was performed using ToyoScreen SuperQ-650M column (TOSOH, Yamaguchi, Japan) built up with AKTA purifier (GE healthcare ...
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bioRxiv - Biophysics 2020Quote: ... The di-nucleosome DNA with the modified 5S rRNA gene cassettes (X5S 217F-2) was excised with PvuII and purified by ion-exchange chromatography on a TSK-gel DEAE-5PW column (Tosoh Corporation). In the naked DNA transcription assay ...
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bioRxiv - Biochemistry 2022Quote: ... and the cleaved actin and thymosin β4-His6 moiety were separated by ion-exchange chromatography with SuperQ-5PW resin (Tosoh Bioscience, Japan) using a 0-0.5 M NaCl gradient in G-Buffer ...
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bioRxiv - Immunology 2023Quote: ... comprising anion-exchange chromatography on TOYOPEARL GigaCap Q-650M (Tosoh Bioscience) column ...
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bioRxiv - Biophysics 2021Quote: ... K420B and K420C were further purified via anion exchange chromatography using a TSKgel SuperQ-5PW (Tosoh bioscience) 7.5 mm ID x 7.5 cm column equilibrated in HB buffer (35 mM PIPES-KOH pH 7.2 ...
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bioRxiv - Biophysics 2022Quote: ... Holo-OCP was subsequently separated from apo-OCP using anion exchange chromatography (DEAE 650 M) (Tosoh, Japan) by performing a 0-100 mM NaCl linear gradient ...
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bioRxiv - Biophysics 2023Quote: Dynactin was purified from pig brains using SP Sepharose Fast Flow and MonoQ ion exchange columns (Cytiva) and the TSKgel G4000SWXL size exclusion column (Tosoh), as previously described 70 ...
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bioRxiv - Microbiology 2021Quote: ... The S6 or S15 proteins were purified by anion-exchange column (Tosoh) and Hitrap Capto Core400 (Thomas Scientific) ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... pre-embedded with 100 µL Toyopearl cation exchange media SP 650M (Tosoh) for each well ...
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bioRxiv - Microbiology 2020Quote: ... and size-exclusion chromatography on TSKgel G4000SWXL (TOSOH). We also expressed a recently reported spike protein construct with 4 additional proline substitutions that enhance thermostability ...
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bioRxiv - Immunology 2021Quote: ... followed by size-exclusion chromatography on TSKgel G4000SWXL (TOSOH) if needed.
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bioRxiv - Synthetic Biology 2023Quote: Gel Permeation Chromatography (GPC) TSKgel SuperHZM-H column (Tosoh) connected in series with a DAWN multiangle laser-light scattering (MALLS ...
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bioRxiv - Microbiology 2023Quote: ... and then the recombinant proteins were purified with nickel-chelated affinity chromatography (Tosoh), dialyzed with phosphate-buffered saline (PBS) ...
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bioRxiv - Cell Biology 2023Quote: ... Dynein complexes were further purified by fast protein liquid chromatography (TSKgel G4000SWxl column [TOSOH Bioscience]) and concentrated by centrifugation through an Amicon Ultra-4 Centrifugal Filter Device (Merck Millipore) ...
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bioRxiv - Physiology 2023Quote: ... HbA1c was measured by high-performance liquid chromatography (Tosoh Medics, Inc., San Francisco, CA, USA). In the CU study ...
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bioRxiv - Physiology 2023Quote: ... HbA1c was measured by high-performance liquid chromatography (Tosoh Medics, Inc., San Francisco, CA, USA) or with a handheld analyzer.
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bioRxiv - Biochemistry 2019Quote: ... to 500 µL and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience) with GF150 buffer (25 mM HEPES [pH7.4] ...
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bioRxiv - Cell Biology 2021Quote: ... to 500 µL and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience) with GF150 buffer (25 mM HEPES [pH7.4] ...
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bioRxiv - Cell Biology 2022Quote: ... to 500 µl and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience) with GF150 buffer as the mobile phase at 1 ml/min ...
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bioRxiv - Immunology 2020Quote: ... and separated by Hydrophilic-Interaction Liquid Chromatography (HILIC) using a TSKgel Amide-80 column (Tosoh Bioscience). Glycan species were identified by relative retention time and quantified using autointegration of each glycan species peak.
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bioRxiv - Cell Biology 2021Quote: ... to 500 μL and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience) with GF150 buffer (25 mM HEPES [pH7.4] ...
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bioRxiv - Biochemistry 2023Quote: ... to 500 µl and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience) with GF150 buffer (25 mM HEPES (pH 7.4) ...
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bioRxiv - Microbiology 2024Quote: Gel-Permeation Chromatography (GPC) was carried out in chloroform on a TSKgel SuperHZM-H column (Tosoh) with a DAWN MultiAngle Light Scattering (MALS ...
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bioRxiv - Cell Biology 2024Quote: The eluates were further purified using size exclusion chromatography with a TSKgel 5000PW column (Tosoh Bioscience) in 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: Nanodisc reconstituted ABCD1 was further purified by size exclusion chromatography (SEC) on a G4000swxl SEC column (TOSOH biosciences) pre-equilibrated with reconstitution buffer on an Agilent 1260 Infinity II LC system (Agilent Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... The dimer-rich LH1-RC fractions were collected and further purified by gel-filtration chromatography (TSKgel G4000SW, 7.5 mm I.D. × 60 cm, TOSOH) with 20 mM Tris-HCl buffer (pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... followed by further purification on a StrepTrap HP column (Cytiva) and size-exclusion chromatography on TSKgel G4000SWXL (TOSOH). To express the RBD subdomain of the SARS-CoV-2 S protein ...
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bioRxiv - Bioengineering 2020Quote: ... Cy5-cetuximab-MMAE conjugates were analysed by hydrophobic interaction chromatography-HPLC using an TSKgel Ether-5PW column (Tosoh Biosciences). Antibody-drug isomers were separated from unconjugated cetuximab by the HPLC method using a linear gradient from 100 % high salt concentration buffer A (0.05 M sodium phosphate buffer ...
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bioRxiv - Biophysics 2022Quote: ... The eluted ABCA7 nanodiscs were concentrated using a 100,000 MWCO kDa Amicon filter and further purified by size exclusion chromatography using a G4000swxl column (TOSOH biosciences) equilibrated with nanodisc buffer at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Another aliquot of each digest was subjected to hydrophilic interaction chromatography (HILIC) on an Amide-80 column (TOSOH, Tokyo, Japan) to collect glycopeptides ...
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bioRxiv - Neuroscience 2021Quote: Plasma concentration of kynurenine (Kyn) was measured by high-performance liquid chromatography (HPLC) with a spectrophotometric detector (TOSOH UV-8000) or fluorescence spectrometric detector (HITACHI ...
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bioRxiv - Molecular Biology 2021Quote: ... The affinity columns were prepared from an in-house prepared GMP stock of MAb PGT145 by coupling of the antibody to Toyopearl 650M chromatography resin (Tosoh). Loaded columns were washed with ≥ 10 GV of 20 mM Tris / 500 mM NaCl / pH 8.3 and bound ConM and ConS trimers were eluted from the columns with 50 mM histidine / 2 M MgCl2 / pH 6.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... NP-high performance liquid chromatography (HPLC) was performed using an TSK gel Amide-80 column (4.6× 250 mm; Tosoh Co.) and a Waters 2690 Alliance HPLC separation module (Waters Co. ...
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bioRxiv - Immunology 2022Quote: The purity of S-Trimer was analyzed by Size-Exclusion Chromatography (SEC-HPLC) using Agilent 1260 Infinity HPLC with an analytic TSK gel G3000 SWxL column (Tosoh). Phosphate Buffered Saline (PBS ...
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bioRxiv - Biochemistry 2021Quote: ... the samples were fractionated by size-exclusion chromatography (SEC) on a TSKgel SuperSW2000 column (300 mm × 4.5 mm × 4 μm, Tosoh Bioscience), which was operated at 200 μL/min in 30% ACN ...
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bioRxiv - Molecular Biology 2021Quote: ... The purity of sHDLs was analyzed by gel permeation chromatography (GPC) using a Tosoh TSK gel G3000SWxl column (Tosoh Bioscience) with UV detection at 220nm.
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bioRxiv - Immunology 2020Quote: The purity of S-Trimer was analyzed by Size-Exclusion Chromatography (SEC-HPLC) using Agilent 1260 Infinity HPLC with an analytic TSK gel G3000 SWxL column (Tosoh). Phosphate Buffered Saline (PBS ...
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bioRxiv - Biophysics 2022Quote: ... 1.4 M ammonium sulfate was added and then applied to a hydrophobic interaction chromatography column with a Toyopearl Butyl-650S-substituted matrix (Tosoh Bioscience) using a peristaltic pump ...
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bioRxiv - Molecular Biology 2020Quote: ... 80 μL of the reconstituted sample was injected and fractionated by hydrophilic interaction liquid chromatography (HILIC) using a TSK gel Amide-80 column (2 mm x 150 mm, 5 μm; Tosoh Bioscience). Three solvents were used for the gradient ...
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bioRxiv - Immunology 2020Quote: ... Size exclusion high performance liquid chromatography: Purified hMAbs (10 µg) were injected into a TSKgel G3000SWXL column equipped with a TSKgel guard column SWXL (Tosoh Biosciences) using an Agilent 1100 Series HPLC ...
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bioRxiv - Microbiology 2020Quote: ... and separated by high performance liquid chromatography (HPLC) on an amide-80 column (4.6mm ID x 25cm, 5 μm, Tosoh Bioscience, Tokyo) with a 50 mM ammonium formate/acetonitrile buffer system ...
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bioRxiv - Neuroscience 2022Quote: ... The fragment between AscI and FseI sites was digested and purified through size exclusion chromatography using Toyopearl HW-75 resin (Tosoh Bioscience). The purified fragments were injected into through standard pronuclei injection at the LASF of Stowers Institute.
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bioRxiv - Biochemistry 2019Quote: ... reconstituted in 80% acetonitrile and 1% formic acid and fractionated by hydrophilic interaction liquid chromatography (HILIC) with TSK gel Amide-80 column (2 mm x 150 mm, 5 µm; Tosoh Bioscience). 90sec fractions were collected between 10 and 25 min of the gradient ...
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bioRxiv - Immunology 2021Quote: ... The RBD-Fc protein was purified by affinity chromatography with the AktaPure system with a protein A column (TOYOSCREEN AF-RPROTEIN A HC-650F; Tosoh Bioscience). Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... and their purity and conjugation efficiency were assessed via gel permeation chromatography (GPC, EcoSEC Elite, Tosoh Biosciences, King of Prussia, PA) with refractive index (RI) ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM DTT buffers containing 500 mM KCl and 1 M KCl were pooled together and further purified using size exclusion chromatography with a TSKgel 5000PW column (Tosoh Bioscience) in 25 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... and injected into a high-performance liquid chromatography system (LC-10AD, Shimadzu, Kyoto, Japan) with TSKgel ODS-80TM (4.6 × 250 mm, 5 µm, TOSOH Corporation, Tokyo, Japan) to quantitate the daidzein ...
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bioRxiv - Molecular Biology 2023Quote: ... were injected on an Agilent 1200 series highperformance liquid chromatography (HPLC) instrument and separated using a TSKgel G3000SWxl sizeexclusion column (Tosoh Bioscience #08541). The mobile phase was 100 mM sodium phosphate ...