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Citations for Roche :
701 - 750 of 3706 citations for ssc mir 17 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Quantitative PCR was performed using Kapa SYBR fast PCR kit (Kapa Biosystems) in a Realplex 4 PCR machine (Eppendorf) ...
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bioRxiv - Immunology 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with SYBR green (Roche) in a StepOnePlus system (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... PCR was carried out with the KAPA HiFi PCR Kit (KAPA Biosystems) in a total volume of 25 μl ...
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bioRxiv - Systems Biology 2023Quote: ... Real-time PCR was conducted using the SYBR green PCR reagent (Roche) and StepOnePlus™ System (Applied Biosystems ...
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bioRxiv - Bioengineering 2024Quote: ... and PCR amplification was performed with KAPA2G Robust PCR kits (KAPA Biosystems) as described above ...
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bioRxiv - Microbiology 2020Quote: ... and 5 µg of a WEAU gp160 plasmid (pcDNA3.1-WEAU gp160) into exponentially dividing 293T/17 cells using FuGENE 6 (Roche Applied Science, Indianapolis, IN) or into 293F cells using 293fectin (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... Reverse transcription was performed adding 2 μl of DNAse-treated RNA to 17 μl reaction mixture containing 1X Expand Reverse Transcriptase Buffer (Roche Diagnostics, Mannheim, Germany), 10mM of Dithiothreitol (DTT ...
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bioRxiv - Developmental Biology 2021Quote: ... RT-qPCR was performed using KAPA SYBR® FAST Master Mix (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and library concentration was quantified with KAPA Library Quant KIT RT-qPCR (Roche). Total RNAseq was done as 100nt paired-end sequencing and performed using the Illumnia platform (HiSEQ4000 ...
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bioRxiv - Genomics 2020Quote: ... RT-qPCRs were performed using 1× LightCycler 480 SYBR Green I Master (Roche), 0.5 μM primers (Suppl ...
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bioRxiv - Genomics 2021Quote: ... and by RT-qPCR using the KAPA library quantification kit (#KK4835, Kapa Biosystems), according to the manufacturers’ protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... RT-qPCR was performed on a LightCycler® 480 II (Roche Life Science) using SYBR® Premix Ex Taq(tm ...
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bioRxiv - Developmental Biology 2022Quote: ... RT-qPCR was performed using KAPA SYBR FAST qPCR Kit (Kapa Biosystems, KK4602) according to manufacturer’s instructions with the following cycler programme ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was subsequently performed as described [23] using Sybr green (Roche, 06924204001) on The LightCycler 480 System ...
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bioRxiv - Genetics 2022Quote: ... RT-qPCR was performed using the Kapa SYBR FAST qPCR kit (Kapa Biosystems) and StepOnePlus™ Real-time PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2022Quote: ... RT-qPCR was performed using LightCycler 480 SYBR Green I master mix (Roche). For quantification of gene expression ...
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bioRxiv - Immunology 2022Quote: ... slides were blocked at RT for 1 h in 1x Blocking reagent (Roche) and incubated at 4°C over night with a phospho-histone H2AX (pSer139 ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR assays were carried out on LightCycler LC480 II (Roche Diagnostics, Germany) using SYBR Green Jumpstart Taq Ready mix (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR was performed using KAPA SYBR FAST qPCR master mix (Kapa Biosystems) with gene-specific primers (Supplementary Table 6 ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed in a LightCycler® 2.0 instrument (Roche, Mannheim, Germany), using 45 cycles of the following program ...
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bioRxiv - Physiology 2020Quote: ... Reverse transcription was performed with RT Transcriptor First Strand cDNA Synthesis Kit (Roche) with anchored oligo-dT primers (Roche) ...
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bioRxiv - Genetics 2019Quote: ... RT-qPCR reactions were carried out in quadruple on a LC480 machine (Roche). The reaction mix consisted of cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... a minimum of 50ng of RNA was used to prepare cDNA (Roche RT) and splice isoform specific PCRs were performed (Sigma Aldrich High fidelity Taq ...
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bioRxiv - Immunology 2023Quote: ... The RT-qPCR was run on a Roche LightCycler 480 (Roche Diagnostics Ltd) using the conditions outlined in Table 4.
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bioRxiv - Biophysics 2022Quote: GGCAGCGCTACCATAACGGA-3’) (11) by RT-qPCR was performed using LightCycler 480 II (Roche). GAPDH mRNAs were used as a loading control (14) ...
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bioRxiv - Plant Biology 2022Quote: ... Expression analysis by RT-qPCR was performed using SYBR Green master I (Roche) and the LightCycler® 96 system following a standard protocol (40 cycles ...
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bioRxiv - Cell Biology 2023Quote: ... RT-qPCR was performed with the LightCycler® 480 Instrument (Roche Life Science) and data were analyzed according to manufacturer’s instruction (Roche LightCycler® 480 software ...
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bioRxiv - Molecular Biology 2023Quote: ... The RT-qPCR was run on a LightCycler 480 II (Roche Applied Science) using 384 well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... RT-qPCR was performed using the SYBR Green I Master kit (Roche, 04887352001) following the manufacturer’s recommended protocol and a Bio-Rad CFX96 qPCR Real-Time PCR Module with C1000 Touch Termal Cycler Unit ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed using LightCycler 480 SYBR Green I Master mix (Roche) and run in a LightCycler 480 System (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of selected genes was measured by RT-qPCR (LightCycler 480; Roche), cDNA was prepared from 1 µg of RNA using QuantiNova Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Developmental Biology 2023Quote: ... RT qPCR samples were prepared using the KAPPA SYBR R fast (Roche, KK4611). Primer Sequences:
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was conducted on a LightCycler 480 II Detection System (Roche, Switzerland) with TransStar Tip Top Green qPCR Supermix (Transgen Biotech ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR was then performed using SYBR green master mix (Roche, Indianapolis, IN) and a Light Cycler 480 Instrument II (Roche ...
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bioRxiv - Microbiology 2024Quote: ... and RT-qPCR was performed using SYBR green master mix (Roche, Indianapolis, IN) and a Light Cycler 480 Instrument II (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... The RT-qPCR cycling analysis was performed using a LC-480 device (Roche). qBasePlus software 3.2 (www.biogazelle.com ...
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bioRxiv - Microbiology 2020Quote: ... Cobas PCR Media (Roche); Aptima Specimen Transport Medium (Hologic) ...
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bioRxiv - Genomics 2022Quote: ... and quantitative PCR (Roche), according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2023Quote: ... PCR Free (KAPA Biosystems). Adaptor ligated DNA was treated with Lambda Exonuclease (NEB ...
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bioRxiv - Immunology 2023Quote: ... PCR-free (Roche, #KK8503) with KAPA Unique-Dual Indexed (UDI ...
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bioRxiv - Cell Biology 2020Quote: ... and the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). Average size fragment was determined using a LabChip GX (PerkinElmer ...
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bioRxiv - Genetics 2021Quote: ... and transcript-specific primer pairs (Table S1) on the LightCycler® 96 System (Roche). The ΔΔCt method was used to calculate the relative quantification of transcripts [70] ...
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bioRxiv - Molecular Biology 2021Quote: ... and 0.6 μM of forward and reverse primers with a LightCycler® 480 (Roche). The primer pairs used for different RT-qPCR reactions are listed in Table 2 ...
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bioRxiv - Genomics 2019Quote: ... ssDNA was converted to dsDNA using 50 pmol of random hexamer primers phosphate (Roche) as previously described (Cadoret et al ...
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bioRxiv - Microbiology 2019Quote: ... and a KAPA qPCR MasterMix plus Primer Premix kit (Kapa Biosystems, Woburn, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Probes and primers (Supplementary Table S1) designed with the UPL System Assay Design (Roche) were tested for at least 95-105% efficiency ...
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bioRxiv - Genomics 2020Quote: ... 0.2 μM of each primer and 1.5 U KAPA Taq DNA polymerase (Kapa Biosystems). The thermal profile of the PCR included 40 cycles of denaturation at 95°C for 30 s ...
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bioRxiv - Neuroscience 2020Quote: ... Intron spanning qPCR primers were designed with the universal probe library software from Roche. Expression of each gene was normalized to beta-actin ...
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bioRxiv - Biochemistry 2020Quote: ... Primers used in the reactions were designed in LightCycler® Probe Design v2 (Roche) software ...
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bioRxiv - Evolutionary Biology 2020Quote: Primers (0.5 mM each) were mixed with the KAPA2G Robust HotStart ReadyMix (Kapa Biosystems) inside a DNA/RNA UV-Cleaner cabinet (UVC/T-AR) ...