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Citations for Roche :
101 - 150 of 3664 citations for mmu mir 212 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR (qRT-PCR) analysis was performed using LightCycler Quick System 350S (Roche Applied Science, Penzberg, Germany) with Thunderbird SYBR qPCR Mix (Toyobo) ...
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bioRxiv - Genomics 2020Quote: ... 10 uM primers and the KAPA HiFi 2x PCR Master Mix (KAPA Biosystems) with the following conditions ...
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bioRxiv - Plant Biology 2022Quote: ... The qRT-PCR primers were designed by Universal Probe Library Design Center (Roche) (Table S3) ...
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bioRxiv - Biochemistry 2023Quote: ... mega-primers were amplified by PCR reactions (KAPA HiFi DNA Polymerase, Roche, Switzerland) with specific primers bearing mutations and the reverse primer overlapping the integration sites in the destination vector ...
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bioRxiv - Genomics 2022Quote: ... Empty/filled PCRs (combining 5′ and 3′ flanking primers) and full-length PCRs (using junction-spanning primers) were performed using an Expand Long Range dNTPack (Roche, Cat#: 4829034001). Reaction mixes contained 5μL 5× Expand Long Range Buffer with 12.5mM MgCl2 ...
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bioRxiv - Molecular Biology 2022Quote: ... two PCR reactions were performed with PCR anchor primer (included in the 2nd Generation 5’/3’ RACE Kit, Roche) and GB3 (oligo 61 ...
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bioRxiv - Molecular Biology 2021Quote: ... and analysed by 25-cycle amplification using Titan One Tube RT-PCR (Roche) with primers 5’-GGC GCG GTC GGT AAA GTT-3’ and 5’-AGC GTT TTC CCG GTA TCC A-3’ (luciferase ...
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bioRxiv - Genomics 2021Quote: ... Quantitative RT-PCR was performed using the LightCycler 480 System (Roche Life Science) with the following conditions ...
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bioRxiv - Biochemistry 2021Quote: ... was measured in a LightCycler 480 LC RT-PCR system (Roche, Basel, Switzerland) over the temperature range 20-95 °C with a temperature ramp rate of 2.4 °C/min ...
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bioRxiv - Neuroscience 2021Quote: ... RT-qPCR was performed using the Real-Time PCR System (LightCycler 96, Roche). Each reaction comprised of 0.5 µL of diluted cDNA ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative RT-PCR analyses were carried out in a Light Cycler 96 (Roche) real time PCR detection system using KAPA SYBR FAST qPCR Master Mix (KapaBiosystems) ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR was performed with Lightcycler 480 SYBR Green I Master (Roche) in a total volume of 5 µL and analyzed on a Lightcycler 480 (Roche) ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative RT-PCR was performed by amplification with SYBR green Master Mix (Roche). The primers used are listed in Table 1 ...
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bioRxiv - Immunology 2020Quote: ... The reaction was performed in a LightCycler 480 (384) RT-PCR platform (Roche), using the LightCycler 480 Multiwell 384-well plates (04729749 001 ...
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bioRxiv - Cell Biology 2020Quote: ... TaqMan RT-PCR was performed using TaqMan Master Mix and Light Cycler96 (Roche). Oligonucleotides are listed in supplementary Table 1.
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bioRxiv - Molecular Biology 2023Quote: ... Quantification of the RT-PCR product was obtained on a LightCycler 480 (Roche).
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bioRxiv - Cell Biology 2023Quote: ... The RT product was amplified by LightCycler 480 Real-Time PCR system (Roche) using PowerUp SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative real time PCR (RT-qPCR) was performed on a LightCycler 480 (Roche). cDNAs were synthesized from 500ng of total RNA using the SuperScript™ III Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... Genes were amplified by q-RT-PCR using a Roche Lightcycler 96 (Roche).
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bioRxiv - Cancer Biology 2023Quote: ... Real-time quantitative RT– PCR was performed on a LightCycler 2.0 apparatus (Roche) using the Light Cycler FastStart DNA Master SYBERGreen I kit (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... using the Transcriptor One-Step RT PCR Kit (Roche Applied Science, Mannheim, Germany). Sanger-sequencing (Sequencer 3130 Genetic Analyzer ...
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bioRxiv - Immunology 2020Quote: ... High-resolution HLA typing was performed by next-generation sequencing on a GS Junior Sequencer using the GS GType HLA Primer Sets (both Roche, Basel, Switzerland). HLA typing was successful for HLA-A ...
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bioRxiv - Molecular Biology 2022Quote: ... Junction qPCRs were set up by dispensing crude gDNA and premixed junction primer pairs into a LightCycler® 1536 Multiwell Plate (Roche 05358639001) containing 1x LightCycler® 1536 DNA Green mix (Roche 05573092001) ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 genome equivalents (GE) were detected by quantitative RT–PCR (LightCycler 480 Real-Time PCR System and Software version 1.5 (Roche)) ...
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bioRxiv - Developmental Biology 2022Quote: The different CRE candidate sequences were amplified using PCR-specific primers (see Table S2) and the Expand High Fidelity PCR system (Roche). Amplified fragments were gel-purified and cloned into the pSK-lacZ vector as in (Beccari et al. ...
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bioRxiv - Microbiology 2020Quote: ... DNA probes for 5S rRNA and SPA0079 were amplified with PCR using primers described in Table S2 and PCR DIG Probe Synthesis Kit (#11636090910, Roche). The reaction was performed with a total volume of 50 μl containing 0.2 μM of forward primer ...
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bioRxiv - Microbiology 2022Quote: ... A twenty-fold dilution of the first PCR was used as template for a second PCR with primers Anchor (Roche) and sp3R for czcR cDNA or sp2C for czcC cDNA ...
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bioRxiv - Genomics 2022Quote: ... Re-purified circular fragments were amplified by PCR with View-Point specific primers (see Supplementary Table 2) using the Expand Long Template PCR System (Roche). Resulting amplicons were purified with a 0.8× ratio of AMPure XP beads (Beckman Coulter ...
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bioRxiv - Plant Biology 2023Quote: ... Paromomycin probe was labeled with dUTP-DIG during PCR reaction (PCR DIG probe synthesis kit, Roche; see table primers list). Membrane was pre-hybridized 1h at 50°C in a DIG eazy Hyb (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... The digoxigenin (DIG)-labeled probes were generated by PCR on H26 genomic DNA using primers and the PCR DIG labeling Mix from Roche. Probe hybridization was detected using the DIG Luminescent Detection kit (Roche ...
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bioRxiv - Immunology 2021Quote: ... and PCR amplified using Nextera primers with 2x HiFi Polymerase Master Mix (KAPA Biosystems). Amplified ...
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bioRxiv - Cell Biology 2023Quote: ... amplified using construct-specific PCR primers (KAPA HiFi Hot Start plus dNTPs; Roche; KK2502), and inserted into the pGEMHE plasmid backbone (In-fusion kit ...
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bioRxiv - Microbiology 2024Quote: ... and specific primers (Table 3) on the LightCycler 480 Real-Time PCR System (Roche). Fold change was calculated using the comparative 2-ΔΔCt method ...
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bioRxiv - Cancer Biology 2023Quote: ... or 20 µM miR-122 antagomiR (Miravirsen, SPC-3649, Roche, Mannheim ...
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bioRxiv - Immunology 2024Quote: ... or TaqMan™ Fast Universal PCR Master mix with probe sets from the Universal Probe Library (Roche). The CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... The levels of relevant mRNAs were quantitated by real-time PCR using One Step SYBR GREEN RT-PCR Kit (Roche) in a Light Cycler instrument (Roche Applied Science ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR was performed with FastStart Universal SYBR Green Master Mix and a LightCycler 96 Real-Time PCR System (Roche). Transcript levels of genes were calculated relative to the level of PP2A ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative reverse transcription PCR (RT-PCR) was performed with 10 ng of cDNA in a Light Cycler® Instrument (Roche) using LightCycler® 480 probes master mix (4887301001 ...
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BTBD9 is a novel component of IGF signaling and regulates manganese-induced dopaminergic dysfunctionbioRxiv - Neuroscience 2021Quote: ... Real-time quantitative PCR (RT-qPCR) used 2×RealStar Green Fast Mixture (GenStar) with a Real-time PCR Detection System (LightCycler96, Roche). The following primers were used in the amplification ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative RT-PCR was performed in technical and biological triplicates on a 96-well LightCycler real-time PCR system (Roche) using SYBR Green (Roche) ...
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bioRxiv - Bioengineering 2023Quote: ... Reverse transcription polymerase chain reaction (RT–PCR) was performed on the LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland), and the cycling parameters were 1 cycle at 95 °C for 10 min ...
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bioRxiv - Microbiology 2019Quote: ... The extracted genomic DNA was amplified using the amplicon forward primer and amplicon reverse primer with a Hifi hot start ready mix PCR (Kapa Biosystems, Wilmington, MA, USA), then purified using a fast gene purification kit (Nippon Genetics ...
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bioRxiv - Zoology 2020Quote: A first strand cDNA Synthesis Kit for rt-PCR (Roche Diagnostics GmbH, Mannheim, Germany) was used for first-strand cDNA synthesis of the RNA pooled from different developmental stages of Spadella cephaloptera ...
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bioRxiv - Cell Biology 2019Quote: ... Real-time RT PCR was performed using FastStart Universal SYBR Green Master mix (Roche). Samples were analyzed in triplicate for β2-Microglobulin (B2M) ...
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bioRxiv - Genetics 2019Quote: ... real-time RT-PCR was performed on a LightCycler® Nano (Roche, Mannheim, Germany). The PCR reaction mixture in a 20 μl volume containing 10 μl of 2×SYBR Green PCR Master Mix (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR was performed with a LightCycler 96 Real-Time PCR System (Roche Diagnostics), using HOT FIREPol EvaGreen qPCR Mix (Solis BioDyne ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time RT-PCR was then performed on a LightCycler™ system (Roche Diagnostics) using the light Cycler-DNA Master SYBR Green I mix ...
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bioRxiv - Pathology 2021Quote: ... Quantitative RT-PCR was carried out using SYBR Green master mix (Roche Applied Science) on a Light Cycler LC480 ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative RT-PCR was performed with 20 ng cDNA in a LightCycler instrument (Roche) using FastStart Essential DNA Green Master (Roche 06402712001) ...
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bioRxiv - Genomics 2021Quote: ... and RT-PCR was performed using the SYBR Green Mix (Roche, Nutley, NJ, USA) on LightCycler480 system (Roche) ...