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Citations for Roche :
1 - 50 of 65 citations for cis Clopidogrel MP 13C d3 Derivative Pair of Enantiomers since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... we calculated mean Cp values using the second derivative method (2nd derivative Max) implemented in LightCycler 480 SW 1.5 (Roche Applied Science). Replicate was considered as positive when Cp <36 ...
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bioRxiv - Immunology 2022Quote: ... and Liberase CI (40 μg/ml; Roche) at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... The second derivative maximum method in the LightCycler 480 software (Roche) was utilized to obtain a quantification cycle (Cq ...
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bioRxiv - Immunology 2021Quote: ... and 0.2mg/ml Liberase CI purified enzyme blend (Roche Diagnostics Corp.), and incubated for 1–2hours at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Tissue was then digested with 250 mg/ml liberase CI (Roche) and 500 mg/ml DNase I (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... mRNA amplification was analysed by second derivative maximum algorithm (LightCycler 480 Sw 1.5; Roche) and expression of the gene of interest was normalised to the geometric mean of three housekeeping genes Rn45s ...
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bioRxiv - Immunology 2023Quote: ... mRNA amplification was analysed by second derivative maximum algorithm (LightCycler 480 Sw 1.5; Roche) and expression of the gene of interest was normalised to a combination of housekeeping genes as specified in each figure.
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bioRxiv - Microbiology 2020Quote: ... and 0.2 mg/ml Liberase CI purified enzyme blend (Roche Diagnostics Corp.), and incubated for 1-2 h at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... The CI-MPR was purified by anti-HPC4 affinity agarose (Roche, 11815024001) following the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2022Quote: ... Cycle threshold values were quantified as the second derivative maximum using LightCycler software (Roche, Switzerland). The 2−ΔΔCt method31 was used to analyze RT-qPCR data and determine relative quantification corrected for primer efficiency ...
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bioRxiv - Developmental Biology 2022Quote: ... Cycle threshold values were quantified as the second derivative maximum using LightCycler software (Roche, Switzerland). Noncoding small RNA control sno202 served as an endogenous reference gene [30] ...
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bioRxiv - Cell Biology 2020Quote: ... [U-13C]palmitate was first non-covalently conjugated to ultra fatty acid free BSA (Roche) as previously described (Vacanti et al. ...
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bioRxiv - Bioengineering 2020Quote: ... Primer pairs were designed using the Universal Probe Library by Roche (https://lifescience.roche.com/en_de/brands/universal-probe-library.html#assay-design-center ...
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bioRxiv - Immunology 2020Quote: ... PCR amplification was analysed by the second-derivative maximum algorithm (Light Cycler 480 Sw 1.5; Roche), and expression of the gene of interest was normalized to that of the housekeeping gene Actb (beta-actin) ...
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bioRxiv - Neuroscience 2020Quote: ... Crossing points of amplified products were determined using the Second Derivative Maximum Method (Light Cycler Version LCS480 1.5.0.39, Roche). Quantification of messenger RNA expression was relative to receptor expression-enhancing protein 5 (REEP5 ...
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bioRxiv - Molecular Biology 2022Quote: ... CP values were calculated using the Second Derivative Maximum method implemented in the LightCycler 480 Software (Roche). CT values were calculated using the Comparative CT (ΔΔCT ...
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bioRxiv - Microbiology 2023Quote: ... using the second-derivative-maximum method provided by the Light Cycler quantification software (version 3.5 (Roche Diagnostics)) or using CFX Maestro (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... CP values were calculated using the Second Derivative Maximum method implemented in the LightCycler 480 Software (Roche). CT values were calculated using the Comparative CT (ΔΔCT ...
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bioRxiv - Cell Biology 2023Quote: ... Primary-secondary-antibody pairs were as follows: Ki67 (Roche Tissue Diagnostics, 05278384001), OmniMap-anti-Rb HRP (Roche Tissue Diagnostics) ...
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bioRxiv - Genomics 2022Quote: ... Ct values were determined using the second-derivative maximum algorithm implemented in LightCycler software (Roche Applied Science, Germany). Index PCR was performed with the same conditions as real-time PCR ...
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bioRxiv - Microbiology 2024Quote: ... placed in RPMI containing 0.2 μg/mL Liberase CI purified enzyme blend (Roche Diagnostics Corp.), and incubated for 1 h at 37 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Expression data derived from Cp values calculated according to the second derivative maximum method (LightCycler®LC480 II, Roche) and normalized to the expression of PDF2 (At4g04890) ...
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bioRxiv - Genetics 2021Quote: ... and transcript-specific primer pairs (Table S1) on the LightCycler® 96 System (Roche). The ΔΔCt method was used to calculate the relative quantification of transcripts [70] ...
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bioRxiv - Systems Biology 2020Quote: ... Crossing point (CP) values were calculated using the second derivative maximum method of the Light Cycler 480 software (Roche Diagnostics). An internal dilution series of template cDNA (stimulated for 1 hour with 500 U/ml IFNα ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were incubated with 8-keto-NeuAc derivatives for 24 h and proliferation measured using WST-1 (Roche Applied Science) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Ct values were determined by 2nd derivative maximum analysis in the LightCycler 480 Software v 1.5.1.62 SP3 (F. Hoffmann-La Roche AG, Basel, Switzerland). The averaged Ct values of technical triplicates were normalized using the mean GAPDH expression and calibration using normalized expression values in hiPSC was performed ...
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bioRxiv - Cell Biology 2024Quote: ... Relative quantification of mRNA transcripts was calculated based on an objective method using the second derivative maximum algorithm (94) (Roche). All target genes were normalised to two reference genes (HPRT1 and B2M ...
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bioRxiv - Biophysics 2023Quote: ... 5% d8-13C depleted glycerol, 1 mM EDTA, 5 ug/mL of aprotinin and leupeptin and 100 µg/mL Roche protease inhibitor cocktail ...
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bioRxiv - Immunology 2023Quote: ... The tissue was minced finely in the digest containing RPMI with 0.1 mg/ml liberase CI (Roche) and 1mg/ml DNase I (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2024Quote: ... The cycle time values were determined using the second derivative maximum method (Tichopad et al., 2003) via LightCycler software (version 1.5, Roche Life Science). The data were analyzed using the comparative threshold cycle (Ct ...
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bioRxiv - Genetics 2024Quote: ... Total RNA was extracted from 5-8 pairs of ovaries using an RNA extraction kit (Roche). 50 ng of total RNA was used to make cDNA using the Transcriptor First Strand cDNA Synthesis kit (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... and qPCR analysis was carried out using specific primer pairs and SYBR green master mix (Kapa Biosystems) with a QuantStudio 5 Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... and PcAEV5-3’ (nt 11,547-12,331) were prepared with specific primer pairs (Table S1) using PCR DIG Labeling Mix (Roche). Riboprobes were also prepared using the DIG RNA Labeling Kit (SP6/T7 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were considered not infected when no Cp value was calculated using Absolute quantification/2nd derivative Max analysis on the Light Cycler® 480 (Roche, Software release 1.5.1.62 SPS).
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bioRxiv - Genomics 2021Quote: ... a junction PCR was performed using an intron-spanning primer pair (Junction_fwd and Junction_rev) and 8 μl cDNA with the KAPA HotStart HiFi Ready Mix (KAPA Biosystems) for 15 cycles ...
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bioRxiv - Genetics 2020Quote: ... Customized oligonucleotide probes were designed to capture the exons and 30 base pairs of adjacent sequences using NimbleGen (Roche) online oligonucleotide probe design system.
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bioRxiv - Molecular Biology 2021Quote: ... PCR was performed with Kapa Hifi HotStart polymerase using conditions recommended for a <500 base pair (bp) product (Kapa Biosystems, Roche Molecular Systems ...
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bioRxiv - Molecular Biology 2021Quote: ... Sublibrary oligo pools were amplified individually using window-specific primer pairs and KAPA HiFi HotStart ReadyMix (Roche, Indianapolis, IN, USA) with a final template concentration of 1:2000 resuspended oligo array and annealing temperatures of either 60 °C or 65 °C (depending on performance of individual primer pairs ...
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bioRxiv - Immunology 2023Quote: ... Real-time PCR was performed using qPCR primer pairs listed in Table S2 and LightCycler480 SYBR Green I Master Mix (Roche) in a LightCycler480 instrument ...
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bioRxiv - Neuroscience 2022Quote: ... Libraries were base-pair selected based on Agilent 2100 Bioanalyzer profiles and normalized determined by KAPA Library Quantification Kit (Roche). The libraries were built from 3 to 5 different mice per genotype ...
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bioRxiv - Microbiology 2024Quote: ... Primer pair mix with 7 µL of 2X SYBR green (LightCycler 480 SYBR Green I Master; Roche; Cat No. 04707516001) and 1 µL of 10 µM of forward and reverse primer were prepared for all the gene targets ...
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were conducted with 50 ng of genomic DNA and following primer pairs spanning the sgRNA target site in KAPA HiFi HotStart ReadyMix (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... using 1:50 of the reverse-transcribed RNA sample mixed with a specific primer pair (1uM final each, sequence in Table 4) and 2X KAPA SYBR FAST qPCR Master Mix (Roche) according to manufacturer’s conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR was performed with Kapa Hifi HotStart polymerase using conditions recommended for a <500 base pair (bp) product (Kapa Biosystems, Roche Molecular Systems ...
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bioRxiv - Neuroscience 2021Quote: ... The sequences of the specific forward and reverse primer pairs were constructed using the Primer-BLAST tool or using the “Universal Probe Library” software (Roche Diagnostics). Sequences of the different primer pairs used are listed in Supplementary Table S1 for humans and Supplementary Table S2 for rats ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were ligated to the mate pair fragments and 8 cycles of PCR was used to enrich for the final library (KAPA Biosystems). The prepared libraries were quantified using KAPA Biosystems’ next-generation sequencing library qPCR kit and run on a Roche LightCycler 480 real-time PCR instrument ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Illumina compatible adapters (IDT, Inc) were ligated to the mate pair fragments and amplified with 8-12 cycles of PCR (KAPA Biosystems).
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bioRxiv - Developmental Biology 2021Quote: ... was carried out using 10 ng of cDNA and 100 nM of each primer pair with a Roche LightCycler 480 machine and LightCycler 480 SYBR Green I Master 2X (Roche, 04887352001). The PCR program was ...
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bioRxiv - Microbiology 2022Quote: The V4 hypervariable region of the 16S rRNA gene was amplified with the primer pair 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3) on a Fast Start High Fidelity PCR System (Roche, PQ) using the following conditions ...
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bioRxiv - Cell Biology 2024Quote: ... PCR reactions were conducted with 100 ng of genomic DNA and following primer pairs spanning the sgRNA target site in KAPA HiFi HotStart ReadyMix (Roche KK2602) according to the manufacturer’s instructions ...