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Citations for Roche :
1 - 50 of 895 citations for Zika Virus VLP E prM M Proteins since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 3 μm sections were stained with H&E and Masson trichrome (Trichrome II Blue staining kit at Nexus special stainer; Roche). Immunohistochemical analysis was performed on cryosections and paraffin sections using a Bench Mark XT automatic staining platform (Ventana ...
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bioRxiv - Microbiology 2022Quote: ... influenza virus hemagglutinin (Roche), followed by 1:4000 anti-rat horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2022Quote: ... libraries were constructed from 2.5 ng VLP DNA using a KAPA HyperPrep Kit (KAPA Biosystems) with 12 cycles of amplification ...
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bioRxiv - Immunology 2023Quote: ... Stimulated cultures were lysed in M-PER mammalian protein extraction reagent supplemented with PhosphoSTOP (Roche) and cOmplete Tablets (Roche ...
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bioRxiv - Microbiology 2021Quote: ... or LightCycler Multiplex RNA Virus Master (Roche). SYBR green based RT-qPCRs were performed using Luna Universal One-Step RT-qPCR Kit (NEB).
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bioRxiv - Evolutionary Biology 2020Quote: ... The protein extraction was performed by adding 10 M urea containing complete protease inhibitor cocktail (Roche) and Halt™ Phosphatase Inhibitor (Thermo ...
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bioRxiv - Cell Biology 2023Quote: ... HBE were processed for protein lysates and stored at -20°C (Complete Lysis-M Roche diagnostics). NET dosage was an average of extracellular dsDNA concentration (QuantiFluor ONE dsDNA System ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed and proteins were purified by Complete™ Lysis-M EDTA-free kit (Roche, 04719964001). Protein samples were run on Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... cells/tissue were lysed in mammalian protein extraction reagent (M-PER) supplemented with protease inhibitor cocktail (Roche). Soluble proteins and insoluble matrix were separated by centrifugation at 10,000 × g ...
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bioRxiv - Molecular Biology 2019Quote: ... avian myeloblastosis virus reverse transcriptase (AMV RT; Roche) were used to synthesize cDNA from total RNA (2.5 µg ...
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bioRxiv - Microbiology 2020Quote: ... or LightCycler® Multiplex RNA Virus Master (Roche) using CFX96 Real-Time System ...
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bioRxiv - Microbiology 2022Quote: ... or LightCycler® Multiplex RNA Virus Master (Roche) using the CFX96 Real-Time System ...
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bioRxiv - Physiology 2019Quote: ... The Cobas e 601 (Roche, Germany) electrochemiluminescence device was used to determine the concentrations of serum FSH ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed in 8 M urea buffer (8 M urea, 1 M Tris pH 7.4, 5 M NaCl) containing protease and phosphatase inhibitors (Roche) followed by sonication at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated in staining solution (1 M Tris-HCl pH 9.5, 5 M NaCl, 1 M MgCl2, NBT-BCIP, H2O) (Roche) in the dark for 10 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The Realtime Ready RNA Virus Master (Roche, Cat # 05619416001) was used to amplify viral RNA with the following primers/probe ...
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bioRxiv - Immunology 2021Quote: Cells were collected in RIPA buffer (0.5 M EDTA, 1 M Tris-Cl pH7.5, 1 M NaCl, 200 mM, Roche protease inhibitor) at 0h ...
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bioRxiv - Cancer Biology 2024Quote: ... or protein extraction (50 mM Tris-HCl, 1% NP40, 0.15 M NaCl, pH 8) supplemented with phosphatase (PhoStop, Roche) and protease inhibitors (Complete ...
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bioRxiv - Cell Biology 2022Quote: ... HUVEC were seeded to confluency on the microelectrodes of the E-plate (E-plate 16, Roche Applied Science). Electrical impedance readings were taken every 2 min for 5h ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 800 μg of VLP lysate was then reduced and alkylated as described above and treated with 5 U PNGase F (Roche Cat. Nr. 11365177001) at 37 °C for 12 hours followed by 12 hours with 11 μg of trypsin ...
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bioRxiv - Developmental Biology 2023Quote: ... HUVEC were seeded at a density of 60,000cells/well of the E-plate (E-plate 16, Roche Applied Science), then electrical impedance readings acquired every 2 min for 24 hr ...
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bioRxiv - Neuroscience 2020Quote: ... MSNs were harvested in M-PER Mammalian Protein Extraction Reagent (Pierce) with cOmplete Mini EDTA-free protease inhibitor (1 tablet/10 mL) (Roche). Whole-cell lysates were sonicated with a 5 second pulse followed by a 5 second rest (X 5 times ...
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bioRxiv - Cancer Biology 2020Quote: Protein extracts were prepared from drug-treated cells by lysis on ice for 20 minutes in M-PER Mammalian Protein Extraction Reagent (ThermoScientific) supplemented with protease and phosphatase inhibitors (Roche). Lysates were centrifuged at 16,000 RPM for 20 minutes at 4°C and the supernatants were assessed for concentration via a Bradford assay ...
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bioRxiv - Microbiology 2020Quote: Western blot analysis of infected and uninfected erythrocytic material performed on protein extracts prepared by resuspending cells in M-PER mammalian protein extraction reagent (Pierce) supplemented with 1x protease inhibitory cocktail (EDTA free) (Roche), 300 mM benzamidine ...
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bioRxiv - Microbiology 2020Quote: ... For IVD approved LightCycler® Multiplex RNA Virus Master (Roche) 5 μl of template RNA were used ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in M-PER mammalian protein extraction reagent (Thermo, MA, USA) with protease and phosphatase inhibitors (Roche, Basel, Switzerland), and proteins were quantified using protein assays (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2019Quote: ... cells were incubated for 48 h at 37°C in 5% CO2, harvested and lysed using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78501) (with 1X Roche Complete Protease Inhibitor and 100 mM NaCl) ...
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bioRxiv - Microbiology 2022Quote: ... For the IVD-approved LightCycler® Multiplex RNA Virus Master (Roche), 5 µL of template RNA in a total reaction volume of 20 µL was used ...
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bioRxiv - Cell Biology 2023Quote: ... with virus packaging and envelope expressing plasmids using Fugene-6 (Roche). Medium was replaced with DMEM with 10% FCS the next day and supernatant was harvested 3 days post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Virus passages were then amplified in penicillin-streptomycin-containing media (Roche) until cells became visibly swollen and demonstrated reduced viability statistics ...
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bioRxiv - Cell Biology 2021Quote: Total protein from cells or mouse tissues (n=3 per genotype) were extracted using the M-PER protein lysis buffer (ThermoScientific, Beverly, MA) containing protease inhibitors (Roche, Indianapolis, IN). Approximately 25 μg of total protein was electrophoresed on 4-12% SDS-PAGE gels and transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with PBS and lysed (200 µl) with M-PER Mammalian Protein (ThermoScientific®) plus 1x Protease inhibitor (cOmplete Mini EDTA, Roche®). Cells were detached using a scraper (on ice) ...
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bioRxiv - Cell Biology 2020Quote: ... HUVEC were pre-treated with siRNAs or drug for 24 hr prior to plating an equal cell number onto the microelectrode surface of the E-plate (E-plate 16, Roche Applied Science). Impedance readings were taken automatically every 5 min for 24 hr.
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bioRxiv - Microbiology 2022Quote: ... using the LightCycler Multiplex DNA Virus Master kit (Roche Diagnostics, Mannheim, Germany) on a LightCycler 480 instrument (Roche Diagnostics).
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bioRxiv - Evolutionary Biology 2019Quote: Dengue virus was quantified via RT-qPCR using the LightCycler 480 (Roche). We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... including 5 μL template using LightCycler Multiplex RNA Virus Master (Roche, Switzerland). All assays were carried out in triplicate on the Roche Lightcycler 96 platform (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus was produced in 293T cells transfected using X-tremeGENE HP (Roche) with viral constructs along with VSV-G and gag-pol ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR analysis was conducted using anti-SARS-CoV-2 primer pairs specific to the E-gene (Corman et al. 2020) and Lightmix Modular SARS and Wuhan CoV E-gene (Roche Lifescience, Basel, Switzerland) in an ABI 7500 Fast PCR cycler (PE applied biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... Forebrains were finely chopped and then homogenized in 0.32 M sucrose solution (0.32 M sucrose, 10 mM Hepes pH 7.4, with cOmplete protease (Roche) and phosphatase inhibitors ...
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bioRxiv - Developmental Biology 2021Quote: ... 5000 cells were seeded in a 96 E-plates (Roche) and the electrical impedance was acquired every 15 minutes for seven days to establish a cell index value extracted from the linear regression of the proliferation curve ...
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bioRxiv - Cell Biology 2022Quote: ... pH 7.5) supplemented with e-complete protease inhibitor cocktail (Roche) was used as lysis buffer ...
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bioRxiv - Immunology 2023Quote: ... A cobas e 411 analyzer ® (Roche Diagnostics, Indianapolis, IN) measured endogenous estrogen (E2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µg·mL-1 E-64 and protease inhibitor cocktail (Roche). For polysome dissociation ...
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bioRxiv - Bioengineering 2023Quote: ... 3 % NaCl (5 M, v/v), 0.08 % CaCl2 (2.5 M, v/v) and cOmpleteTM ULTRA Protease Inhibitor Cocktail tablets (Roche) in H2O ...
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bioRxiv - Plant Biology 2024Quote: ... 0.5 M EDTA and Protease Inhibitor (Roche). The cell lysate was collected by centrifuging the homogenate at 20,000g for 10 min ...
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bioRxiv - Genetics 2020Quote: ... Cells pellets were combined and lysed in a denaturing urea buffer (8 M urea, 0.1 M ammonium bicarbonate, pH 8, 150 mM NaCl, 1 Roche mini protease inhibitor tablet without EDTA/10 ml ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1.5 ml 5 M NaCl, 12.5 μL 2 M Spermidine, final volume brought to 50 ml with dH2O, complemented with one Roche Complete Protease Inhibitor EDTA-Free tablet) ...
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bioRxiv - Molecular Biology 2024Quote: ... The remaining supernatant was diluted in 1 mL pre-warmed capturing buffer (0.5 M LiCl, 4 M urea, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche) and 10 µL from an equimolar stock (10 µM ...
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bioRxiv - Pathology 2020Quote: ... The measurements were done on a Cobas e 311 (Roche, Switzerland). The inter-assay coefficient of variation was <1.65% using a frozen serum pool and <1.66% using quality control samples from Roche.
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bioRxiv - Pathology 2020Quote: ... a sandwich electrochemiluminescence immunoassay ECLIA on Cobas e 411 (Roche, Switzerland). The inter-assay coefficient of variation was < 5.06% using a frozen serum pool and < 2.36% using quality control samples from Roche ...