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Citations for Roche :
601 - 650 of 1965 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Single cell dissociation was performed through enzymatic digestion (5mg/ml collagenase (Roche, 57981821) and 200U/ml hyaluronidase (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... erythrocytes were lysed in 2 mL red blood cell lysis buffer (Roche, 11814389001) for 5 min at room temperature before the addition of 10mL AdDF+++ and centrifugation at 400 rcf ...
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bioRxiv - Cell Biology 2023Quote: Cells were collected and lysed in RIPA buffer (Pierce) containing protease inhibitors (Roche). Extracts were sonicated ...
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bioRxiv - Cancer Biology 2023Quote: ... CRISPRa- and CRISPRi-expressing cells were selected with hygromycin (0.8 µg/ml, Roche). Lentivirus was produced in 293 cells by co-transfecting the lentiviral constructs with pCMV-dR8.2 (Addgene plasmid #8455 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed in RIPA buffer supplemented with a protease inhibitor cocktail (Roche). Whole cell extracts were resolved by SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with X-tremeGENE 9 DNA Transfection Reagent (Roche, Basel, Switzerland) following manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: Cell proliferation evaluation was determined using the WST-1 colorimetric assay (Roche, Germany). ECM hydrogels were prepared in wells of 48 well plates ...
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bioRxiv - Cell Biology 2023Quote: ... Apoptosis was evaluated using the cell death detection ELISA kit (Roche, Basel, Switzerland). HUVEC were trypsinized ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated in ice-cold RIPA buffer containing cOmplete protease inhibitor (Roche) for 10 minutes with rotation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The cell proliferation ELISA BrdU (5′-bromo-2′-deoxyuridine) colorimetric assay (Roche,11647229001) was performed as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: RNA was extracted from HeLa cells (conditions 1,2,3) using TriPure® (Roche®) according to manufacturerś protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Electroporated (EP) cells were then selected with 400 ug/mL Neomycin (Roche, #04727878001) and resistant clones (mES cell line expressing Cre ...
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bioRxiv - Cell Biology 2023Quote: Plasmids containing a sgRNA were transfected into HeLa cells with X-tremeGENE8 (Roche). Single GFP-positive cells were sorted by fluorescence-activated cell sorting (FACS ...
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bioRxiv - Immunology 2024Quote: ... Cell nuclei were stained with 5 µg/ml of DAPI (10236276001, Roche Diagnostics).
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bioRxiv - Cancer Biology 2024Quote: ... and digested into single cells (tissues) using a mix of collagenase/dispase (Roche), accutase (Millipore) ...
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bioRxiv - Microbiology 2024Quote: ... and incubated with the In Situ Cell Death Detection Kit Fluorescein mix (Roche) at 37°C for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed in CERI buffer containing the Complete Protease Inhibitor Cocktail (Roche). Cells were vortexed on the highest setting for 15 seconds and incubated on ice for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... cell nuclei were counterstained using 40,60-diamidino-2-phenylindol (DAPI, Roche, Mannheim, Germany). Labeled cells were washed three times with TBS before being covered with Fluoromount mounting medium (Southern Biotech ...
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bioRxiv - Cancer Biology 2024Quote: Cells were lysed in RIPA buffer supplemented with a protease inhibitor cocktail (Roche) and phosphatase inhibitor (Cell Signaling 5870s) ...
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bioRxiv - Cancer Biology 2021Quote: ... All human cell lines were provided by the Roche Non-Clinical Biorepository from Roche Basel or the Roche-Innovation Center Zurich (RICZ) ...
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bioRxiv - Cell Biology 2020Quote: ... and single islet cells were obtained from islets using Dispase II (Roche, Indianapolis, USA) digestion ...
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bioRxiv - Cell Biology 2020Quote: ... siRNA treated HeLa cells were lysed with RIPA buffer containing protease inhibitor (Roche, #11836170001) for 30 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... The concentration of the 10x single cell library was determined via qPCR (Kapa Biosystems). The libraries were normalized ...
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bioRxiv - Microbiology 2021Quote: ... Disruption cell was achieved with sonication after addition of antiprotease EDTA-Free cocktail (Roche) and 30U/ml benzonase (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: Cells were lysed in RIPA buffer supplemented with protease and phosphatase inhibitors (Roche, #4906837001) for protein extractions and separated by polyacrylamide gel electrophoresis using 4-12% gradient gels (Novex by Life Tech ...
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bioRxiv - Immunology 2021Quote: ... cells were collected in RIPA lysis buffer containing both protease and phosphatase inhibitors (Roche) and standardised to 1 μg/μl as mentioned above ...
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bioRxiv - Molecular Biology 2021Quote: ... Briefly, cells were lysed in lysis buffer (2% SDS, 1X protease inhibitor cocktail (Roche), and 1 mM sodium azide in 1X TBS ...
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bioRxiv - Developmental Biology 2021Quote: ... BrdU incorporation was determined colorimetrically with the Cell proliferation ELISA kit (Roche, Basilea, Switzerland) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed twice in 50mL PBS with protease inhibitors (Complete EDTA free, Roche) before being pelleted and flash frozen in liquid nitrogen and stored at −80C ...
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bioRxiv - Molecular Biology 2020Quote: ... HT1080 cells were incubated with 20 µM EdU and 100 ng/mL colcemid (Roche) for 75 min to arrest cells in metaphase ...
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bioRxiv - Immunology 2021Quote: Thymic cell suspensions were obtained via enzymatic digestion of thymic lobes using Liberase (Roche) and DNaseI (Roche) ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lysed in RIPA buffer supplemented with protease and phosphatase inhibitors (Roche, #4906837001) for protein extractions and separated by polyacrylamide gel electrophoresis using 4-12% gradient gels (Novex by Life Tech ...
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bioRxiv - Microbiology 2019Quote: HEK 293 cells were transfected with pcDNA4T/O-3FLAG::susF using FuGENE HD (Roche) transfection reagent according to manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... 500,000 cells from limb buds were then dissociated with 100ug/mL Liberase (Roche 05401119001), resuspended in CSKT buffer (10mM PIPES pH6.8 ...
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bioRxiv - Immunology 2020Quote: ... The cells were plated in pMCPECs media on 50ug/ml laminin (Roche, Mannheim, Germany) coated inverted filters of 5μm pore size and 6mm diameter (Corning ...
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bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA was extracted using the DNA Isolation Kit for Cells and Tissues (Roche), according manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2019Quote: ... The cell monolayer was then washed with PBS containing cOmplete Protease Inhibitor Cocktail (Roche), collected by scraping and concentrated by centrifugation (2000xg ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were lysed in HR lysis buffer supplemented with protease inhibitor cocktail (11836145001; Roche). Samples were kept on ice and lysed by sonication (10 cycles of 30 seconds on and 30 seconds off) ...
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bioRxiv - Physiology 2020Quote: Epithelial cells were isolated from piglet tracheas by enzymatic digestion (Pronase, Roche; DNase, Sigma) seeded onto collagen (Rat Tail Corning Collagen I ...
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bioRxiv - Biochemistry 2019Quote: ... Bacterial densities were counted with a CASY-Model TT cell counter and analyzer (Roche Innovatis AG ...
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bioRxiv - Developmental Biology 2019Quote: ... The tissues were stained with an In Situ Cell Death Detection Fluorescein kit (Roche) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... cells were lysed in 1% SDS containing PBS supplemented with protein inhibitor cocktail (Roche) and 250 U/ml bensonase (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated with rat anti-HA antibodies (Roche, 3F10; 0.2 μg/ml) for 1 hr ...
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bioRxiv - Developmental Biology 2020Quote: ... For TUNEL staining using the “In Situ Cell Death Detection Kit TMR” (Roche #12156792910), embryos were fixed for four hours in Immunobuffer ...
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bioRxiv - Molecular Biology 2020Quote: Cells were lysed in ice-cold RIPA buffer containing a protease inhibitor cocktail (Roche) before cellular debris was cleared by centrifugation at max speed in a refrigerated tabletop centrifuge ...
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bioRxiv - Immunology 2021Quote: Cell pellets were resuspended in RIPA buffer supplemented with Complete Protease Inhibitor Cocktail (Roche), incubated for 30 minutes on ice and sonicated using a Bioruptor Plus sonicator ...
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bioRxiv - Bioengineering 2022Quote: ... TUNEL staining was performed using the In Situ Cell Death Detection Kit (Roche, 11684795910) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... Cells were lysed on ice with sonication after which DNAse I (1 μL, Roche) and MgCl2 (to 1 mM ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were lysed on ice with sonication after which DNAse I (1 μL, Roche) and MgCl2 (to 1 mM ...
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bioRxiv - Cancer Biology 2020Quote: ... Vectors were transfected into HEK293FT cells using X-tremeGENE 9 DNA Transfection Reagent (Roche). Retroviral supernatants isolated at 48 h were diluted 1:1 in culture medium and used to infect the ARID1A-wildtype ES2 and KLE cell lines ...