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Citations for Roche :
651 - 700 of 1389 citations for U Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Amplification plots were analyzed using the LightCycler 96 System Application Software (Roche). Gene expression was quantified by comparing the fluorescence generated by each sample with a standard curve of known quantities ...
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bioRxiv - Cancer Biology 2020Quote: ... or using a LightCycler® 96 (Roche Applied Science, Indianapolis, IN, USA) with FastStart SYBR Green Master Mix (Roche Applied Science ...
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bioRxiv - Microbiology 2021Quote: ... on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics, Indianapolis, IN). Viral RNA concentration was determined by interpolation onto an internal standard curve composed of seven 10-fold serial dilutions of a synthetic ZIKV RNA fragment based on a ZIKV strain derived from French Polynesia that shares >99% similarity at the nucleotide level to the Puerto Rican strain used in the infections described in this manuscript.
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bioRxiv - Microbiology 2021Quote: ... Thermal cycling was carried out using a LightCycler 96 instrument (Roche Diagnostics) under the following conditions ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed with the Light Cycler 96 (Roche, Geneva, Switzerland) using iQ SYBR Green Supermix (170-8882AP ...
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bioRxiv - Biochemistry 2020Quote: ... The following method was used in a LightCycler 96 (Roche, Indianapolis, IN): 3 steps amplification (45 cycles ...
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bioRxiv - Cell Biology 2019Quote: ... The cDNA was amplified using a fluorescence ratio PCR instrument (Roche 96). The final volume of the solution was 20 μl ...
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bioRxiv - Cell Biology 2020Quote: ... in a LightCycler® 96 Real-Time PCR System (Roche Life Science). Primers ...
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bioRxiv - Microbiology 2020Quote: ... on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics, Indianapolis, IN). Viral RNA concentration was determined by interpolation onto an internal standard curve composed of seven 10-fold serial dilutions of a synthetic ZIKV RNA fragment based on a ZIKV strain derived from French Polynesia that shares >99% similarity at the nucleotide level to the Puerto Rican strain used in the infections described in this manuscript.
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bioRxiv - Plant Biology 2022Quote: Quantitative RT-PCR was performed using a LightCycler 96 (Roche, Basel, Switzerland). Thunderbird SYBR qPCR Mix (Toyobo ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR reactions were performed on a LightCycler 96 (Roche, Mannheim, Germany) using qPCRBIO SyGreen Mix (PCR Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... with 100 ng RNA per sample on a LightCycler 96 instrument (Roche). PCR primers for Rpl8 and Ifna4 were used as given above together with the universal probe library probes (UPL ...
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A laboratory framework for ongoing optimisation of amplification based genomic surveillance programsbioRxiv - Genomics 2023Quote: ... with MagNA pure 96 DNA and Viral NA Small Volume Kit (Roche). cDNA was generated for all samples using LunaScript RT SuperMix Kit (New England BioLabs) ...
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bioRxiv - Cancer Biology 2023Quote: ... using GAPDH as an endogenous control on LightCycler 96 PCR system (Roche) using Faststart Essential DNA master mix (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... was performed according to the manufacturer’s instructions on LightCycler 96 (Roche, Germany). Data were evaluated by ΔΔCT method with normalization against Actin levels.
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bioRxiv - Microbiology 2022Quote: ... on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics, Indianapolis, IN). The viral RNA concentration was determined by interpolation onto an internal standard curve composed of seven 10-fold serial dilutions of a synthetic ZIKV or SPONV RNA fragment ...
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bioRxiv - Physiology 2023Quote: ... qRT-PCR was carried out with Lightcycler 96 (Roche Diagnostics, Basel, Switzerland), using KAPA SYBR Fast qPCR kit (NIPPON Genetics Co. ...
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bioRxiv - Systems Biology 2023Quote: ... The qPCR reactions were carried out on a LightCycler®96 (Roche). The rrfD gene (5S rRNA ...
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bioRxiv - Physiology 2023Quote: ... Transcript levels were measured by real-time PCR (Light Cycler 96; Roche), taking advantage of the TaqMan and Sybr Gene Expression Assay System (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2023Quote: ... We measured RT-qPCR reaction on a LightCycler® 96 Instrument (Roche) using the SYBR-green channel and with cycling conditions as follows ...
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bioRxiv - Synthetic Biology 2023Quote: ... We analysed RT-qPCR reaction using the LightCycler® 96 Software (Roche). We calculated viral genome concentration (Vg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... The following qPCR protocol was used on the LightCycler® 96 (Roche): 3 min of activation phase at 95°C ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescence of SYBRgreen was measured using LightCycler 96 (Roche Diagnosis, Vilvoorde, Belgium).
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bioRxiv - Cancer Biology 2024Quote: ... 10 μL of 2× Sybr Green (LightCycler 96, Roche Molecular Systems, Inc.), 200 nM of each primer ...
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bioRxiv - Developmental Biology 2020Quote: ... iWAT depots were digested in PBS containing collagenase D (Roche, 1.5 U/ml) and dispase II (Sigma ...
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bioRxiv - Pathology 2019Quote: ... 1.5 μg RNA was treated with 0.6 U DNAase I (Roche, Basel, Switzerland) for 30 min at 37 °C ...
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bioRxiv - Bioengineering 2020Quote: ... frozen PBMCs were thawed and treated with 10,000 U/ml DNase I (Roche) in RPMI 1640 supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... TdT buffer was combined with terminal deoxynucleotidyl transferase (Roche Diagnostics, 400 U/slide) and Biotin-16-dUTP (Roche Diagnostics ...
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bioRxiv - Neuroscience 2020Quote: ... Pellet was enzymatically digested in collagenase/liberase TL (2 U/mL) (Roche Diagnostics) for 1 h at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... the supernatants were harvested and treated with 37.5 U/ml DNase I (Roche) at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... the supernatants were harvested and treated with 37.5 U/ml DNase I (Roche) at 37 °C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Lymph nodes were incubated in 400 U/ml collagenase D (Roche Applied Science) for 30 min before filtration through a 70 μm filter ...
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bioRxiv - Developmental Biology 2022Quote: ... and 200 U/ml of recombinant human IL-2 (Roche, Nutley NJ, USA). All cell lines were grown in cell culture incubators at 37°C in the presence of 5% CO2.
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bioRxiv - Immunology 2019Quote: ... assays were performed in the presence of 1000 U/mL IL-2 (Roche). Effector-target conjugates were incubated in 200 mL in round-bottom 96-well plates (Corning ...
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bioRxiv - Microbiology 2020Quote: ... the supernatants were harvested and treated with 37.5 U/ml DNase I (Roche) at 37°C for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... Fat depots were digested in PBS containing collagenase D (Roche, 1.5 U/ml) and dispase II (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... 2 mM Na3VO4 and 0.1 U/ml Complete Protease Inhibitor Cocktail Tablets (Roche)) and incubated for 15 mins on ice ...
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bioRxiv - Genetics 2020Quote: ... and incubated with 400 U collagenase D (#11-088-858, Roche applied science) for 30 min at 37°C ...
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bioRxiv - Immunology 2021Quote: ... and digested in RPMI basal medium with 0.14 U/mL Liberase TM (Roche) and 10 U/mL DNase I (Roche ...
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bioRxiv - Genetics 2022Quote: ... 1% NP-40) containing Benzonase (12.5 u/mL) and Protease Inhibitor Cocktail (Roche) and incubated at 4□ for 1 h with rotation ...
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bioRxiv - Microbiology 2023Quote: ... 1X antibiotic-antimycotic) with 100 U/mL recombinant interleukin (IL)-2 (Roche #11147528001) and activated or frozen in freezing media (90% FBS ...
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bioRxiv - Neuroscience 2023Quote: ... mechanically dissociated and digested for 15 minutes with liberase (0.4 U/mL; Roche) and DNAse I (120 U/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... 40 U of RNase inhibitor and with cOmplete TM Protease Inhibitor Cocktail (Roche). For the input for Western blot analysis ...
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bioRxiv - Genomics 2023Quote: ... 6 μL of Ligation-1 mix (3.75 U T4 DNA ligase (Roche, 10799009001), 33.3 mM DTT (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... and recombinant mouse interleukin-2 (mIL-2, 100 U/ml; Roche, Basel, Switzerland), and subjected to flow cytometry as described elsewhere ...
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bioRxiv - Cell Biology 2023Quote: ... 2.4 U/ml Dispase II and 1 mg/ml DNAse-I (Roche Diagnostics) in Ca+Mg+ phosphate buffered saline in GentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... Fragments were digested with RPMI 1640 medium containing Liberase (Roche, 0.05 U/mL) and DNase I (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: Aag2 or U4.4 cells were plated in 48-well plates and transfected with 100 ng of dsRNA per well using 0.4 µL of X-tremeGENE HD (Roche). The medium was refreshed after 3 h and cells were incubated for 48 h ...
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bioRxiv - Cancer Biology 2019Quote: Cells were grown to 200,000 per well in six-well plates and metaphase-arrested using Colcemid (20μl/2ml; Roche #10295892001) for 30min-3h ...
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bioRxiv - Physiology 2022Quote: ... In each reaction 50 ng luciferase reporter and 150 ng expression vectors were transfected into C2C12 myoblasts in 24-well plates (50,000 cells per well) using X-tremeGENE 9 (Roche). 5 ng modified pRL null plasmid (25 ...