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Citations for Roche :
451 - 500 of 6001 citations for Trypsin 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... N-Glycans were enzymatically released by peptide N-glycosidase F (E.C. 3.5.1.52; Roche Applied Science) digestion then purified by C18-Sep-Pak (Waters Corp. ...
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bioRxiv - Evolutionary Biology 2021Quote: ... a second blocking step was performed followed by the addition of an anti-Digoxigenin-POD (11207733910, Roche) antibody that was incubated overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... DIG Washing and DIG Blocking Buffers were prepared using the DIG Wash and Block Set (Roche, USA). The blot was washed twice with Washing Buffer for 10 minutes at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Panagene) diluted in hybridization solution (10 mM Tris-HCl pH 7.2, 70% formamide, 0.5% blocking solution (Roche)) was applied onto the slides followed by incubation at 80°C for 5 min and at room temperature for 2 h ...
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bioRxiv - Developmental Biology 2022Quote: ... slides were incubated with Blocking buffer II containing 0.1% Anti-DIG-AP fab anti body (Roche, 11093274910) overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... pH 7.5) and placed in RBR buffer (20% Goat serum, 2% Roche Blocking Reagent in 1x MAB) on the orbital rocker for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolated cortices were incubated in 500 μl 0.05% Trypsin-EDTA + 0.02 mg/mL DNase I (Roche) for 15 minutes at 37°C ...
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bioRxiv - Biochemistry 2021Quote: The LLPS model in solution was made by (PR)12 peptide and poly-rA RNA (Roche) in water or PBS18 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells fixed with formaldehyde were permeabilised with 0.1% Triton in PBS and then incubated with blocking reagent (Roche) or 2-5% BSA for 30 min at room temperature ...
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bioRxiv - Genetics 2019Quote: ... After 3h at 4°C slides were washed and blocked with 0.5x blocking buffer (1.5% BSA, 5% goat serum, 0.05% Triton X-100) containing protease inhibitors (Roche 1836153) for 1h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were blocked with 10 mL of 1x blocking solution diluted in 1x Maleic Acid Buffer (Roche, 115857262001) with 0.3% TWEEN 20 for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Separated proteins were transferred to PVDF membranes (GE Water & Process Technologies) and treated with Western blocking reagent (Roche) for overnight at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... The experiments with no barcodes were performed in phosphate buffered saline (1x PBS buffer, pH 7.4) supplemented with 0.01% (w/v) Roche Blocking solution (Roche) at room temperature (23 °C) ...
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bioRxiv - Cell Biology 2019Quote: ... Gel pieces were then rehydrated in 50 mM ammonium bicarbonate and digested with Trypsin (Sequencing Grade, Roche) overnight at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... at 37 °C for 3 hours followed by 19 μg/sample of trypsin (Roche Cat. Nr. 11418025001) at 37 °C for 13 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... PBS was aspirated and 5 ml 0.25% pre-warmed trypsin-EDTA with 10 U/µl DNaseI (Roche) was added and put into a 37°C water bath for 30 minutes with gentle inversion every 5 minutes ...
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bioRxiv - Genetics 2021Quote: ... Then they were incubated overnight at 4°C with primary antibodies diluted in a dilution buffer (0.5% blocking reagent (Roche), 2% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then washed 3 times for 5 min each in PBST and blocked with 1X Blocking Reagent (Roche) in PBST ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were then rinsed in methanol followed by PTX (0.3% Triton X-100 in PBS 1x) and incubated in blocking reagent (Roche) for 1 hour at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... the embryos are rinsed in TBS/0.1% Tween-20 (TBST) then blocked for 2 h in 2% blocking solution (Roche) and incubated O/N in the same solution containing 1:2,500 anti-digoxigenin antibody (Roche) ...
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bioRxiv - Physiology 2021Quote: ... Sections were dried and denatured for 10 min at 80°C in hybridization buffer (0.1M Tris, pH 7.2, 25mM MgCl2, 70% formamide [Sigma-Aldrich, Saint Louis, MO], 5% blocking reagent [Roche] with 1.0 μg/mL of Cy-3-labeled telomere-specific [CCCTAA] peptide nucleic acid [PNA] probe [TelC-Cy3 ...
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were first pre-incubated with 2.4G2 antibody (kindly provided by Louis Boon) for blocking Fc receptors and next stained for surface markers in PBS supplemented with 0.5% BSA (Roche) and 2 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... DIG-labelled cDNA probe was incubated at 95 °C for 10 min and immediately placed in the ice for 5 min before adding to the hybridization buffer (5x SSC, 50 % formamide, 0.02 % SDS, 2 % blocking agent (Roche), DEPC-treated water) ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were incubated overnight at 4° C on a rotator with primary antibody in Western Blocking Reagent (Roche), if not stated otherwise ...
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bioRxiv - Cell Biology 2023Quote: ... Hybridization of the first PNA probe (TelC-Cy3) was then carried out in hybridization solution (70% deionized formamide, 0.25% blocking reagent (Roche), 10 mM Tris-HCl pH 7.2 ...
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bioRxiv - Physiology 2024Quote: ... Sections were dried and denatured for 10 min at 80°C in hybridization buffer (0.1M Tris, pH 7.2, 25mM MgCl2, 70% formamide [Sigma-Aldrich, Saint Louis, MO], 5% blocking reagent [Roche] with 1.0 μg/mL of Cy-3-labeled telomere-specific [CCCTAA] peptide nucleic acid [PNA] probe [TelC-Cy3 ...
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bioRxiv - Cancer Biology 2019Quote: ... Purified carcinoma organoids were dissociated into single cell suspension in 0.05% trypsin with 0.1% EDTA supplemented with 2 U/ml of DNase I (Roche) for 2-3 min ...
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bioRxiv - Neuroscience 2021Quote: ... Trypsin was aspirated and the digestion was stopped by adding 0.5 mg/mL DNase I (Roche, Basel, Switzerland) in microglia medium [Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2020Quote: ... This was followed by 8 min of perfusion with digestion buffer (4mg/mL trypsin, 5mg/mL liberase (Roche) and 0.3mM CaCl2) ...
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bioRxiv - Cell Biology 2022Quote: ... Put the uterine horns into a digestion solution containing 0.2% Trypsin and 0.6% Dispase II (Roche BR, 4942078001) in HBSS ...
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bioRxiv - Immunology 2024Quote: ... and gently digested at 4C in RPMI+10% FBS containing 100mg/mL soy trypsin inhibitor (Roche, cat #10109886001), 250U DNase I (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... fixed with 0.2% gluteraldehyde/4% PFA at room temperature and incubated overnight in hybridization buffer (5% Dextran sulphate, 2% blocking powder from Roche, 5X SSC ...
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bioRxiv - Developmental Biology 2021Quote: After blocking in 20% heat-inactivated sheep serum (Cat. # ZLI-9021, Beijing Zhongshan Jinqiao Biotechnology Company) and 2% blocking reagent (Cat. # 12039672910, Roche) for 1 h ...
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bioRxiv - Molecular Biology 2019Quote: ... and between WT mice treated with either 10 mg/kg of a functional blocking antibody against CDH11 (SYN0012; used with permission from Roche) or an isotype control antibody (IgG2a) ...
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bioRxiv - Immunology 2019Quote: ... Samples were washed in 1X maleic acid buffer with 0.1% Tween-20 (MBST) and then incubated in Roche Blocking Reagent (Roche, 1096176) with 10% heat inactivated sheep serum (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 1 µg of Capture-C library per sample was mixed with mouse COT1 DNA and universal as well as index-specific blocking oligos from SeqCap EZ HE-oligo (Roche). 4.5 µl pool of biotinylated probes (xGen Lockdown Probe Pools from IDT) ...
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bioRxiv - Neuroscience 2022Quote: Mounted coronal cryosections were rinsed in PBS for 3 times (5 min) and thereafter incubated in Blocking Reagent (Roche Diagnostics) for 15 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were then incubated overnight at 4 °C with primary antibody of interest diluted in blocking solution (Western Blotting Reagent, Roche). The appropriate species of HRP-conjugated secondary antibody (from Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... Samples were washed in 1X maleic acid buffer with 0.1% Tween-20 (MBST) and then incubated in Roche Blocking Reagent (Roche, #1096176) with 10% heat inactivated sheep serum (Sigma ...
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bioRxiv - Genomics 2021Quote: ... 1μg of Capture-C library per sample was mixed with mouse COT1 DNA and universal as well as index-specific blocking oligos from SeqCap EZ HE-oligo (Roche). 4.5μl pool of biotinylated probes (xGen Lockdown Probe Pools from IDT) ...
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bioRxiv - Developmental Biology 2021Quote: ... 5X SSC, 100 µg/ml heparin, 100 µg/ml yeast RNA, 0.1% TritonX-100, 0.1% CHAPS, 2% Roche blocking reagent) for more than an hour at 60–65 °C ...
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bioRxiv - Immunology 2020Quote: ... 14 1224PL-01) in hybridization buffer (1M Tris pH 7.2; magnesium chloride buffer; deionised formamide; blocking buffer, Roche; deionised water). DNA denaturation was performed 10 min at 82 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were blocked for 30 minutes in 1% Roche Western Blocking Reagent prior to incubating overnight at 4°C with either anti-FITC with horseradish peroxidase conjugate (Roche) at a 1:2000 concentration or anti-DIG-with horseradish peroxidase conjugate (Roche ...
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bioRxiv - Cell Biology 2019Quote: ... mechanically disintegrated and partially digested in a solution of collagenase and trypsin [2 mg/ml collagenase (Roche or Sigma), 2 mg/ml trypsin (Dutcher Dominique or Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... The reaction was stopped by rinsing the retinas once with Lo-Ovo (DPBS, 1.5% BSA, 1.5% Trypsin inhibitor (Roche Diagnostics), pH 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... The Glu-C treated gel pieces were then dried by SpeedVac and rehydrated in a small volume (15 μL) of 50 mM AmBic supplemented with 0.5 μg of trypsin (Roche) at 37 °C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: The tissue was dissociated in 300 µL of 0.25% trypsin-EDTA solution supplemented with 10 U/mL recombinant DNase I (Roche) for 15 minutes at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... beads were washed into 50 mM Ammonium Bicarbonate (FLUKA-40867) and digested with 0.1ug/ul of Trypsin (ROCHE-11047841001) overnight at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were detached 24 h after transfection with Trypsin-EDTA (0.25% wt/vol) and re-plated onto 10 µg/ml fibronectin (Roche) pre-coated MatTek dishes with glass bottoms ...
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bioRxiv - Immunology 2021Quote: ... membranes were washed three times in TBS-T followed by blocking in 10% (v/v) western blotting reagent (Roche, Basel, Switzerland) for 1–2 h at room temperature ...