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Citations for Roche :
1 - 50 of 1688 citations for Tris 4 pyridin 4 ylethynyl phenyl amine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... before to add chemiluminescent substrate CSPD® (disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2’-(5’-chloro)tricyclo [3.3.1.13,7]decan}-4-yl) phenyl phosphate) (Roche). The chemio-luminescent signal was detected by using a G-Box (Syngene).
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bioRxiv - Microbiology 2024Quote: ... 1ml of CDP-Star® Chemiluminescent Substrate (Disodium 2-chloro-5-(4-methoxyspiro[1,2-dioxetane-3,2′-(5-chlorotricyclo[3.3.1.13.7]decan])-4-yl]-1-phenyl phosphate) (Roche, Cat No. 11685627001) was added to 9ml of DIG-detection buffer and membranes were then incubated with the substrate for 5 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... 4°) in lysis buffer (50 Tris-HCl, 150 mM NaCl, Roche complete EDTA-free protease inhibitor); centrifugation for 30 min at 4° and 21,000xg allowed isolation of the soluble fraction supernatant.
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bioRxiv - Cell Biology 2020Quote: ... Magnetic beads were washed 2 times with lysis buffer and 4 times with washing buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1 mg/ml Pefabloc SC (Roche), and EDTA-free protease inhibitor cocktail (cOmplete Tablets ...
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bioRxiv - Genomics 2021Quote: ... These cells were re-suspended in lysis buffer (4% SDS, 50 mM Tris-HCl [pH 8.0], 10 mM EDTA, 100 mM NaCl) with Protease inhibitor (Roche) and RNase inhibitor ...
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bioRxiv - Microbiology 2024Quote: ... Cells were resuspended in cold extraction buffer (50 mM Tris-HCl pH 7.5 containing 1 mM EDTA, 4 mM DTT, cOmplete Protease Inhibitor Cocktail (Roche), and 1 mM PSMF ...
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bioRxiv - Cancer Biology 2024Quote: ... concentrations was assessed using reduction in WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]- 1,3-benzene Disulfonate) to water-soluble formazan (Roche, France). Cells were seeded in 96-well plates at 2 × 104 cells/well and treated with the different NAM concentrations at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... 4 mg DNAse (Roche) and 4 mM MgCl2 was added and incubated for another hour ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed once with PBS and resuspend with Tris-HCl containing 4 mM EDTA and 1× complete protease inhibitor cocktail (Roche). Dounce homogenizer is used to fractionate the cell membranes and then 3 min 600 g centrifugation was used to obtain Post-Nuclear Supernatants (PNS) ...
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bioRxiv - Cell Biology 2023Quote: Cells were lysed in SDS lysis buffer (4% SDS, 20% glycerol, 125 mM Tris-HCl pH=6.8) supplemented with protease inhibitors (cOmplete ™ Mini (Roche)) ...
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bioRxiv - Genomics 2023Quote: One hundred mg of 14-day-old seedlings were ground and nuclei were isolated with 4°C Buffer (0,25M Sucrose, 10mM Tris-HCl, 10mM MgCL2, 1% Triton, 5mM ß- mercaptoethanol) containing proteinase inhibitor cocktail (Roche) and filtered in 63 µm ...
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bioRxiv - Neuroscience 2020Quote: WB was performed on cell lysates obtained by harvesting cells with lysis buffer (Tris 125 mM pH 6.8, 4% sodium dodecyl sulfate, 20% glycerol) with Complete Protease Inhibitor Cocktail (Roche, Basel, Switzerland) and sonicating ...
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bioRxiv - Developmental Biology 2020Quote: ... 4-toluidine salt (BCIP, Roche) in NTMT buffer ...
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bioRxiv - Microbiology 2023Quote: ... 4 U DNase I (Roche) with the reaction buffer provided with the enzyme for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 mM MgCl2 (Roche Diagnostics), 0.3 µM of each primer (RB1_80F and RB1_235R ...
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bioRxiv - Cancer Biology 2024Quote: ... 4×PIC (Roche; Cat#05056489001)) was added to the pellets ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 mM ATP (Roche, 10519979001)) ...
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bioRxiv - Cancer Biology 2024Quote: ... Version 4 (Roche, Mannheim, Germany), using MagNa Lyser Green Beads (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... NimbleGen 4-plex arrays containing 4 × 72,000 arrays per slide were used (Roche, Mannheim, Germany). Construction of this chip was initially based on combining two previous genome annotations (Amselem et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 μg of plasmid and 4 μl of X-tremeGENE HP DNA Transfection Reagent (Roche) were used per well ...
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bioRxiv - Developmental Biology 2024Quote: ... NBT/BCIP (4-nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolylphosphate, Roche, 11681451001) was added after thoroughly washing samples ...
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bioRxiv - Microbiology 2020Quote: ... Bound proteins were eluted with a 30 minute incubation at 4°C with Elution Buffer (50 mM Tris pH 8.0, 40 mM reduced glutathione, and Roche Complete protease inhibitors). At the end of the incubation ...
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bioRxiv - Genetics 2020Quote: ... Proteins were separated by SDS-PAGE on 4-12% Bis-TRIS SDS-polyacrylamide gels (Novex, NP0322BOX) and analysis was done against primary antibodies α-HA (1:1000, Roche, 12CA5) or α-Tub2 (1:4500 ...
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bioRxiv - Genetics 2020Quote: ... The eluted protein was resolved on a 4-12% Bis-Tris gel (Novex, NP0322BOX) and ubiquitinated Cse4 was detected by Western blot using anti-HA antibody (Roche Inc., 12CA5). Levels of ubiquitinated Cse4 relative to the non-modified Cse4 in the input were quantified using software provided by the Syngene imaging system ...
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bioRxiv - Systems Biology 2023Quote: ... Then the pulverized samples were solubilized with 1000 μL extraction buffer (4% SDS, 100 mM Tris pH 8 supplemented with a cocktail of 1X protease inhibitors (Complete mini, Roche Diagnostics GmbH), 5 mM tris(2-carboxyethyl)phosphine (TCEP) ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were pelleted and resuspended in 2× SDS-PAGE solvent (4% SDS, 20% glycerol, 0.1 M DTT, 0.125 M Tris-HCl pH 7.5 and Roche EDTA-free protease inhibitor cocktail) and boiled at 95°C for 5min ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 tetrazolium]-bis(4-methoxy-6-nitro)-benzene sulfonic acid hydrate (XTT)-based colorimetric assay (Roche) according to manufacturer-recommended protocols ...
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bioRxiv - Developmental Biology 2022Quote: ... and 4 mg/ml dispase (Roche) in complete culture media for 15 min at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... 4 mg/mL DNAase (Roche, 04716728001). The cells were resuspended by mechanical agitation through Pasteur pipettes flamed with decreasing diameters ...
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bioRxiv - Systems Biology 2021Quote: The digested lysates were mixed 1:1 with 2x IP buffer (200 mM Tris, pH 8.0; 600 mM NaCl; 4% Triton X-100; 2x Roche Complete EDTA-free protease inhibitors), and then kept on ice for no more than a few hours prior to antibody addition ...
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bioRxiv - Plant Biology 2021Quote: ... 4 μl of PCR-grade water (Roche), 1 μl of the corresponding primer pair (10 μM each ...
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bioRxiv - Neuroscience 2021Quote: ... 4°C) supplemented with protease (Roche, #11697498001) and phosphatase inhibitors (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... 4 μg/ml Laminin (Roche Basel, Switzerland), and 2 μg/ml Fibronectin (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... Epidermal and dermal sheets were prepared after incubation overnight at 4°C with 4 U/ml dispase II (Roche) in PBS by gently separating dermis and epidermis each as intact sheets (Rahn et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... Signal was revealed with 4 μL of NitroBlue Tetrazolium/5-Bromo-4-Chloro-3-Indolyl Phosphate (NBT/BCIP) Stock Solution (Roche)/ml of AP reaction buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The signals were detected by 4-nitro-blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP, Roche Diagnostics) in a humidified container for 72 h at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... The signals were detected by 4-nitro-blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP, Roche Diagnostics) in a humidified container for 12 h at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... supplemented with 4 ug/ml of insulin (Roche), 15 µg/ml transferrin (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with 4 mg/ml Proteinase K (Roche) in PK buffer (100 mM Tris HCL pH7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... and Biotin-16-dUTP (Roche Diagnostics, 4 μm) and added to slides for 1 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... 4 μg anti-HA antibody (Roche Sigma 11867423001) was used for immunoprecipitation with BioVision immunoprecipitation kit (K286-25) ...
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bioRxiv - Neuroscience 2020Quote: ... and 4-nitro blue tetrazolium chloride (NBT) (Roche) overnight ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM DTT and protease inhibitor cocktail (Roche)) to remove unbound protein ...
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bioRxiv - Cancer Biology 2020Quote: ... containing DNase I (4 U/ml, #4716728001, Roche) at 37 °C for 45 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and 4-nitro blue tetrazolium chloride (Roche Diagnostics) were used for detection ...
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bioRxiv - Neuroscience 2023Quote: ... at 4°C with protease inhibitors cocktail (Roche). After 20 minutes centrifugation at 13000 g at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 mM MgCl2) supplemented with protease inhibitors (Roche cOmplete 1 tablet/10 mL ...
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bioRxiv - Molecular Biology 2023Quote: ... and 4 complete-EDTA protease-inhibitor tablets (Roche) per 500 mL ...
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bioRxiv - Biochemistry 2024Quote: ... and phosphatase inhibitor (Roche; 4-906-837-001) on ice for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... at 4°C with protease inhibitors cocktail (Roche). After 20 minutes of centrifugation at 13000 g at 4°C ...