Labshake search
Citations for Roche :
351 - 400 of 5323 citations for Total Carbohydrate Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... cDNA was synthesized from total RNA with anchored-oligo(dT)18 primer and random hexamer primer using Transcriptor First Strand cDNA Synthesis Kit (Roche, Germany). The template-primer mix was denaturized at 65 °C ...
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bioRxiv - Genetics 2020Quote: ... cDNA was synthesised from the total RNA with anchored-oligo(dT)18 primer and random hexamer primer using Transcriptor First Strand cDNA Synthesis Kit (Roche, Germany). One μg of RNA was added to each 20 μl RT reaction ...
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bioRxiv - Cancer Biology 2020Quote: ... mRNA sequencing libraries were generated from up to 250ng of total RNA and were prepared using a KAPA RNA HyperPrep kit (Roche Diagnostics) with polyA selection and indexed using a KAPA Dual-Indexed adapter kit (Roche Diagnostics) ...
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bioRxiv - Microbiology 2020Quote: ... One μg of intact total RNA per condition was used to make stranded mRNA-seq libraries with the Stranded mRNA-Seq kit (KAPA Biosystems) as per manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Synthesis of cDNA was performed with 150 ng of total RNA using the First Strand cDNA Synthesis kit (Roche Applied Science) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... 250 ng of total RNA was used to prepare barcoded RNA-seq libraries using Stranded RNA-Seq Kit with RiboErase (KAPA Biosystems). Samples were sequenced on the HiSeq 2500 (Figure 2 ...
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bioRxiv - Cell Biology 2022Quote: ... Bulk RNA-seq libraries were prepared from 500 ng of total RNA for each sample using KAPA mRNA HyperPrep Kit for Illumina (Roche, #KR1352) following manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... Library construction of 300 ng total RNA for each sample was made using KAPA Stranded mRNA-Seq Kit (Illumina) (Kapa Biosystems), using 10 cycles of PCR amplification ...
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bioRxiv - Immunology 2023Quote: ... One step real time RT-PCR was performed using 100 ng of total RNA and KAPA SYBR FAST One-Step qRT-PCR Kits (Kapa Biosystems) according to the directions provided by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were constructed by amplifying 500 ng of total RNA using 9 PCR cycles with the KAPA mRNA HyperPrep Kit (KAPA Biosystems). cDNA libraries were validated using the Bioanalyzer 2100 on a High Sensitivity DNA assay and quantified using the KAPA Library Quantification Kit for Illumina Platforms on a Roche Lightcycler 480 ...
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bioRxiv - Cell Biology 2023Quote: Library preparation was performed with 500 ng of total RNA where the ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems), fragmented in reverse transcriptase buffer and heated to 94°C for 8 minutes ...
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bioRxiv - Microbiology 2023Quote: RNA from specimens from Cyprus underwent automated total nucleic acid extraction using the MagNA Pure 96 DNA AND Viral NA Small Volume kit (Roche Diagnostics). RT-PCR for FCoV was performed at Laboklin Bad Kissingen ...
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bioRxiv - Developmental Biology 2024Quote: ... Per sample 200 ng of total RNA was used for library preparations with the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Kapa Biosystems), including steps of the oligonucleotide hybridization ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA sequencing (RNA-seq) libraries were prepared from 4 µg of total RNA using the KAPA stranded mRNA-seq kit (Roche, KK8421) according to manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA-sequencing (RNA-seq) libraries were prepared using 750 ng of total RNA using the KAPA stranded mRNAseq kit (Roche, 8098123702) according to the manufacturer’s specifications ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real time PCRs (qPCRs) were performed in a total volume of 10 µl with Kapa SYBR Fast qPCR kit (KAPA Biosystems) on an ABI 7500 fast machine operated with ABI 7500 software (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... a combination of a branched DNA assay and an ultra-sensitive PCR assay from Roche were used to measure HIV load ...
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bioRxiv - Microbiology 2019Quote: ... All reverse transcriptase qPCR assays for a given target were performed simultaneously for all samples using the LightCycler Multiplex RNA Virus Master Kit (Roche diagnostics). Each sample was normalized with the geometric mean of the quantification cycle (Cq ...
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bioRxiv - Cell Biology 2022Quote: TUNEL assay was performed on N153S and WT lumbar disc tissue sections using an in situ cell death detection kit (Roche Diagnostic). Briefly ...
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bioRxiv - Immunology 2021Quote: ... Quality of libraries was determined via Agilent 4200 Tapestation using a High Sensitivity D1000 ScreenTape Assay kit and quantified by KAPA qPCR (KAPA BioSystems). Approximately 60–80 million paired-end 150 bp reads were generated per sample using Illumina HiSeq 4000 platform ...
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bioRxiv - Cancer Biology 2020Quote: Cell proliferation was measured by determining the extent of 5-Bromo-2’-deoxy-uridine (BrdU) incorporation into DNA of U87-MG cells using the BrdU cell proliferation assay ELISA kit (Roche, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Quantitative expression assays were performed by using the 2x M5 HiPer SYBR Premix EsTag kit (Mei5bio) and a LightCycle®96 (Roche) real-time PCR detection system ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell death was determined by the TUNEL assay using the In Situ Cell Death Detection Kit Fluorescein (Cat # 11684795910; Roche, Germany); only a signal overlapping a DAPI-stained nucleus was considered positive ...
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bioRxiv - Immunology 2023Quote: ... Apoptosis also was detected using a terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end-labeling (TUNEL) assay with Cell Death Detection Kit (Roche, Germany). Nuclei were stained with 4-6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Developmental Biology 2021Quote: ... One microgram of total RNA was reverse transcribed to cDNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics, Tokyo Japan) using an anchored-oligo(dT)18 primer and random hexamer primer ...
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bioRxiv - Genomics 2020Quote: ... a total of 100 ng DNA from each bacterial strain was used according to KAPA Hyper Prep kit (KAPA Biosystems, MA, USA) at the Georgia Genomics and Bioinformatics Core (GGBC) ...
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bioRxiv - Neuroscience 2020Quote: Total genomic DNA was extracted from myoblasts from all participating patients using a High Pure PCR Template Preparation Kit (Roche, Basel, Switzerland).
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bioRxiv - Neuroscience 2020Quote: ... One microgram of the total RNA was reverse-transcribed using the Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics, Florham Park, NJ). qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2019Quote: ... Nucleic acids from these 200μL mosquito pools and from 100μL venous and finger prick whole blood samples in RNAprotect Cell Reagent were isolated using the bead-based MagNAPure LC automatic extractor (Total Nucleic Acid Isolation Kit—High Performance, Roche Applied Science) and eluted in 50μL of water ...
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bioRxiv - Cell Biology 2022Quote: cDNA was synthesized from 100–500 ng of total RNA in 10 µl reactions with a Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland) according to the manufacturer’s protocol using random hexamer primers and an MJ Mini Personal thermal cycler (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: Min6.luc and Min6.CD44 cells were harvested for total RNA isolation using the High Pure RNA isolation kit (Roche Applied Science) and reverse-transcribed using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized by a reverse transcriptase reaction with 500 ng of total RNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche, Basal, Switzerland), and was used as a template for qPCR with LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Strand-specific and barcode-indexed RNA-Seq libraries were generated from 300 ng total RNA each after poly-A enrichment using the mRNA-Seq Hyper Kit (Kapa Biosystems, KK8581) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... A DNA library targe9ng polyadenylated transcripts was constructed for each sample (100ng total RNA) with the KAPA hyper mRNA stranded library prep kit (Roche, catalogue# KK8581) in a 12-cycle PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared by the Van Andel Institute Genomics Core from 500 ng of total RNA using the KAPA stranded mRNA kit (v4.16) (Kapa Biosystems, Wilmington, MA). RNA was sheared to 300-400 bp ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA isolation was performed using the MagNA Pure LC system (Total nucleic acid isolation kit, Roche Molecular System, Laval, QC, Canada) or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0 ...
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bioRxiv - Pathology 2022Quote: ... For WST-1 assay (Roche, 1644807) 200 µL of the working reagent was added per slice and incubated for 45 min in the incubator ...
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bioRxiv - Cell Biology 2023Quote: ... A SYBR Green assay (Roche, Germany) was used to perform qRT□PCR analysis of cDNA templates ...
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bioRxiv - Cell Biology 2019Quote: ... with ATP standard solutions used at concentrations between 10−8 and 10−5 M (luciferin/luciferase ATP bioluminescence assay kit CLSII, Roche, Basel, Switzerland). Data are expressed as nmol ATP produced/min/106 cells.
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bioRxiv - Neuroscience 2022Quote: ... The cell viability following 4-AP treatment was assayed by MTT assay according to manufacturer’s protocols (Roche, cell proliferation kit I (MTT), # 11465007001) ...
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bioRxiv - Physiology 2020Quote: ... Tissues were homogenised in assay buffer provided in the kit with the addition of 5 μL of protease inhibitor cocktail (Roche, Basel Switzerland) and centrifuged at 800 × g for 10 minutes at 4°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and the product was used for the second round PCR using the primers F and R-T7 for generating antisense probe and primers R and F-T7 for generating sense probe used in ISH assays with the DIG RNA Labeling Kit (Roche, Mannheim, Germany),the primers were shown in table 1 ...
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bioRxiv - Developmental Biology 2022Quote: ... in situ terminal deoxynucleotidyl transferase deoxyuridin triphosphate nick end labeling (TUNEL) assay was performed using in situ cell death kit from Roche (Basel, Switzerland).
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bioRxiv - Molecular Biology 2023Quote: ... The proliferative activities of VSMCs were quantified by BrdU incorporation using an enzyme-linked immunosorbent assay (ELISA) detecting kit (Roche, Mannheim, Germany) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: Apoptotic cells present in gonadal histological preparations were revealed by the terminal deoxyribonucleotidyl transferase (TDT)-mediated dUTP-digoxigenin nick end labelling (TUNEL) assay using the Fluorescent In Situ Cell Death Detection Kit (Roche ref. 11684795910, Roche, Mannheim, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: The TUNEL assay was performed to detect apoptosis using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and In-Situ cell death detection Fluorescein kit (Roche Cat#11684795910), as published ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was treated with DNaseI (Roche) and 1µg was converted to cDNA using Super Script III reverse transcriptase (+RT ...
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bioRxiv - Genomics 2020Quote: ... Total RNA was extracted using TriPure (Roche) and further purified using an RNeasy column (Qiagen) ...
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The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate bloodbioRxiv - Cell Biology 2019Quote: ... Total RNA was treated with DNase (Roche) to degrade any genomic DNA contamination ...
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bioRxiv - Biochemistry 2023Quote: ... and 40 µL DNase (Roche; 400U total). The sample was incubated at 37 °C for 1 hour with 250 rpm shaking ...