Labshake search
Citations for Roche :
251 - 300 of 1617 citations for Thyroid Peroxidase TPO Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... and pBlueScript II KS reverse (5’-GCT GGG TCT CGT GGT TTC CCT TTA GTG AGG GTT AAT TG) primers and either 60 µM biotin-16-dUTP or digoxigenin-11-dUTP (Roche). This results in a 5 kb DNA tether which can be attached to a streptavidin coated magnetic bead at one end and an anti-digoxigenin coverslip surface at the other.
-
bioRxiv - Bioengineering 2021Quote: Biotin-labeled dsDNA template was first amplified from the plasmid encoding the template design with biotin-labeled primers using KAPA HiFi HotStart ReadyMix PCR Kit (Roche) for 35 cycles (98°C for 20 s ...
-
bioRxiv - Biophysics 2019Quote: ... The central fragment of the molecule is flanked by oligonucleotides labelled either with digoxigenin (3’ end) or biotin (5’ end) that specifically bind either to a glass surface covered with Anti-digoxigenin (Roche) or to superparamagnetic beads (MyOne ...
-
bioRxiv - Genetics 2021Quote: ... TUNEL (terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling) assay was performed on retinal sections following the manuals (Cat # 11684795910, Roche).
-
bioRxiv - Molecular Biology 2022Quote: ... the fragment of interest was synthesized with Q5 DNA polymerase and handles were synthesized with Taq using either biotin-16-dUTP (11093070910, Roche) or digoxigenin-11-dUTP (11093088910 ...
-
bioRxiv - Molecular Biology 2023Quote: 100 pmol gapmers (Supplemental Table S4) were labeled with biotin-16-ddUTP (Jena) and a 2nd generation DIG-oligonucleotide 3’end-labeling kit (Roche) using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer ...
-
bioRxiv - Genomics 2023Quote: ... GenBank accession number Z69354.1) and BlSat1 (Hong Ha 2018) were labelled by PCR in the presence of biotin-16-dUTP (Roche Diagnostics) detected by streptavidin-Cy3 (Sigma–Aldrich ...
-
bioRxiv - Genetics 2023Quote: DNA fragments of an Alu sequence with T7 promoter on the 5’ end (ssAlu) or both ends (dsAlu) were in vitro transcribed with the biotin RNA-labeling mix (Roche) and T7 transcription kit (Roche) ...
-
bioRxiv - Plant Biology 2023Quote: ... and the probe pXV1 for the 5S rRNA gene (Schmidt et al. 1994) labeled with biotin-11-dUTP by PCR (Roche). The telomeric probe pLT11 from Arabidopsis (Richards & Ausubel 1988 ...
-
bioRxiv - Cell Biology 2024Quote: ... The chromosome V was visualized with a biotin-labelled probe generated by amplifying the endogenous 5s rDNA locus by PCR and labelling was performed with the Biotin-nick translation mix (Roche) according with manufacturer instructions ...
-
bioRxiv - Plant Biology 2019Quote: ... The presence of the proteins of interest was tested by immunodetection using rat anti-HA-peroxidase (3F10, Roche) or chicken anti-c-Myc primary antibody (A2128 ...
-
bioRxiv - Plant Biology 2019Quote: ... water was replaced with 100 μl of a 2 μg/ml horseradish peroxidase (type II; Roche, Rotkreuz, Switzerland) and 5 μM L-012 (WAKO Chemicals ...
-
bioRxiv - Neuroscience 2023Quote: ... brain sections were incubated with horseradish peroxidase (HRP)-conjugated anti-Dig (Roche Applied Science cat#11207733910, 1:500) and anti-GFP (Aves Labs cat#GFP-1010 ...
-
bioRxiv - Plant Biology 2023Quote: ... membranes were cut and immunodetected with either Horse Radish Peroxidase (HRP)-conjugated 3F10 anti-HA (1:2000, Roche) or HRP-conjugated Flag M2 (1:2000 ...
-
bioRxiv - Biochemistry 2023Quote: ... HA-and GFP-tagged proteins were detected using horseradish peroxidase–conjugated monoclonal anti-HA (Roche, 3F10, 1:5,000) and anti-GFP (Roche ...
-
bioRxiv - Plant Biology 2020Quote: ... The sites of hybridization of digoxigenin- and biotin-labeled probes were detected using anti-digoxigenin-FITC (Roche Applied Science, Penzberg, Germany) and streptavidin-Cy3 (ThermoFisher Scientific/Invitrogen ...
-
bioRxiv - Plant Biology 2022Quote: ... Hybridization signals of probes labelled with digoxigenin-11-dUTP and biotin-16-dUTP were detected with antidigoxigenin conjugated to fluorescein isothiocyanate (FITC; Roche Diagnostics) and streptavidin conjugated to Alexa 594 or Alexa 647 (Life Technologies-Molecular Probes) ...
-
bioRxiv - Immunology 2023Quote: ... Apoptosis also was detected using a terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end-labeling (TUNEL) assay with Cell Death Detection Kit (Roche, Germany). Nuclei were stained with 4-6-diamidino-2-phenylindole (DAPI ...
-
bioRxiv - Molecular Biology 2023Quote: Antisense and sense digoxigenin- and biotin-labeled riboprobes of AcerOr11 were synthesized using linearized pGEMHE plasmids containing appropriate insertion sequences as a template using the DIG and Biotin RNA Labeling Mix (Roche, Germany) and T7 RNA Polymerase (Roche ...
-
bioRxiv - Developmental Biology 2021Quote: ... The mRNA expression patterns were visualized by immunoreactivity with anti-digoxigenin horseradish peroxidase-conjugated Fab-fragments (Roche, Basel, Switzerland), according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with horseradish peroxidase (HRP)-conjugated anti-DIG (Roche Applied Science cat#11207733910, 1:500) and anti-GFP (Aves Labs cat#GFP-1010 ...
-
bioRxiv - Neuroscience 2020Quote: The extent of cell death following MPP+ treatment with and without LMX1B overexpression was assessed by using the terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end-labeling (TUNEL) method (In situ cell death detection kit, TMR red, Roche, Cat# 12156792910), according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2020Quote: ... the water was removed with a pipette and 75 μl of Luminol-Mastermix (2 μg/ml horseradish peroxidase (Type II, Roche), 5μM L-012 (WAKO chemicals) ...
-
bioRxiv - Plant Biology 2021Quote: ... Elicitation was performed with the indicated concentration of peptides and 2 μg/ml horseradish peroxidase (Type II, Roche, Penzberg, Germany) and 5μM L-012 (FUJIFILM Wako chemicals ...
-
bioRxiv - Plant Biology 2021Quote: ... water was exchanged for 100 µL water with 5 µM L-012 (WAKO chemicals) and 2 µg/mL horseradish peroxidase (Type II, Roche). After the leaf discs were treated with 1 µM 3-OH-C10:0 (stock in MeOH ...
-
bioRxiv - Developmental Biology 2022Quote: ... before being incubated in TNTw/block for 1 hour followed by an ON incubation with anti-DIG or anti-Fluo horseradish peroxidase (Roche). Post-antibody washes and the TSA reactions were repeated as for the first probe ...
-
bioRxiv - Molecular Biology 2023Quote: ... for the detection of the protein A fragment of the TAP tag or anti-HA peroxidase 1/500 (clone 3F10, Roche).
-
bioRxiv - Plant Biology 2023Quote: ... Proteins expressed from bait and prey vectors were detected by using anti-Myc-tag mAb-HRP-DirectT (MBL, Nagoya, Japan) and anti-HA-Peroxidase 3F10 (Roche), respectively.
-
bioRxiv - Developmental Biology 2023Quote: ... samples were blocked for 30 minutes in 1% Roche Western Blocking Reagent prior to incubating overnight at 4°C with either anti-FITC with horseradish peroxidase conjugate (Roche) at a 1:2000 concentration or anti-DIG-with horseradish peroxidase conjugate (Roche ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein bands were detected using horseradish peroxidase-conjugated and detection with the Lumi- Light Plus Western Blotting Substrate (Roche). Luminescence was detected with an ImageQuant LAS 4000 chemiluminescence imager (GE Healthcare).
-
Proto-sex locus in large yellow croaker provides insights into early evolution of the sex chromosomebioRxiv - Evolutionary Biology 2020Quote: ... The DNA probes constructed from a BAC clone containing dmrt1 (supplementary note 11) were labeled with biotin-11-dUTP using a Nick Translation System (Roche Diagnostics, Basel, Switzerland). The probes were added to the hybridization buffer ...
-
bioRxiv - Genetics 2022Quote: ... 1µg of DNA was labeled with digoxigenin (for chromosome III) or biotin (for chromosome V) by nick-translation according to manufacturer instructions (Roche, 11745816910 and 11745824910).
-
bioRxiv - Cancer Biology 2019Quote: ... Immunostaining was carried out using the BenchMark XT (Ventana Medical Systems, Illkirch Graffenstaden, France) with the OmniMap kit (a “biotin-free” system using multimer technology, Roche, Boulogne Billancourt, France) and a Tris borate EDTA pH8 buffer for antigen retrieval ...
-
bioRxiv - Cell Biology 2023Quote: ... probe was amplified by PCR as described previously (Cox et al., 1993) and labeled using the biotin-nick translation mix (Roche Applied Science, # 11745824910), according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2022Quote: ... Antibodies used: Mouse anti-GFP primary antibody (Roche) at 1:3,000 dilution ...
-
bioRxiv - Neuroscience 2020Quote: ... they were treated with 3% H2O2 for 10 min to inactivate endogenous peroxidases and then closed in 5% bovine serum albumin (BSA; 10735078001, Roche, Switzerland) for 20 min ...
-
bioRxiv - Microbiology 2023Quote: ... one half was immunoblotted with NB13 (7 µg/mL) followed by washing and probing with α-HA-peroxidase (1:1000, Roche), whereas the other half was probed with α-His-peroxidase only (1:4000 ...
-
bioRxiv - Physiology 2023Quote: ... and 1 μL of hemolymph or synthetic HA::spacer::FLAG peptide standard (DYKDDDDKGGGGSYPYDVPDYA) was diluted into 50 μL PBST + 25 ng/mL mouse anti-HA peroxidase (Roche, #12013819001) + 1% non-fat dry milk ...
-
bioRxiv - Developmental Biology 2019Quote: ... These steps were followed by an incubation of the embryos in anti-digoxigenin-POD (peroxidase) Fab fragments (Roche, 1:50 in TNB). The riboprobe signals were finally detected using fluorescein-tyramide (FITC ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated with a solution containing horseradish peroxidase (HRP)- conjugated anti-Dig (at a dilution of 1:500; #11207733910, Roche Applied Science) and goat anti-mCherry (at a dilution of 1:250 ...
-
bioRxiv - Microbiology 2021Quote: ... Western-blot antibody detection was used using antibodies from Roche Diagnostics Mannheim Germany (Anti-HA ...
-
bioRxiv - Cancer Biology 2023Quote: ... Antibodies were diluted in antibody dilution buffer (catalog# 5280524001, Roche Diagnostics ...
-
bioRxiv - Developmental Biology 2019Quote: ... POD antibodies (Roche) in western blocking reagent overnight at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary antibody solution containing anti-DIG-POD antibody (1:250, Roche, RRID:AB_514500) and mouse anti-GFP antibody (1:500 ...
-
bioRxiv - Microbiology 2020Quote: Primary antibody dilutions were used as follows: rat α-HA antibody (Roche) 1:2,000 ...
-
bioRxiv - Cell Biology 2022Quote: ... Primary antibody: rat-derived monoclonal anti-HA antibody (dilution 1:250; Roche). Secondary antibody ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were incubated overnight with primary antibody α HA antibodies (Roche 3F10) (1:1000 dilution in 20% blocking solution I ...
-
bioRxiv - Immunology 2022Quote: ... cells were incubated with anti-TLR7 antibody and anti-HA antibody (Roche) at 37 °C for 90 min ...
-
bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used: monoclonal anti GFP antibody (1:500; Roche), monoclonal anti HA antibody (1:1000 ...
-
bioRxiv - Developmental Biology 2021Quote: ... Fab fragment antibody (Roche) solution in blocking buffer at room temperature for 2 hours with no blocking step ...