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Citations for Roche :
151 - 200 of 374 citations for T 25 Flasks since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... and incubated with anti-c-Myc primary antibody solution (GenScript, 1: 2,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche]) overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... These cells were then lysed in T-PER tissue protein extraction reagent (Pierce) containing protease inhibitor cocktail (Roche) and phosphatase inhibitor cocktail 1(Sigma) ...
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bioRxiv - Plant Biology 2019Quote: ... and incubated with anti-Mouse IgG-HRP secondary antibody solution (Sigma, 1:10,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche]) for 1-2 hrs ...
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bioRxiv - Cell Biology 2022Quote: Troponin T plasma levels were quantified using an electrochemiluminescence immunoassay analyzer “ECLIA” (cobas e 801 immunoanalysis system, Roche) in the hematology laboratory of the Centre Hospitalier Universitaire Vaudois (CHUV) ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.15M NaCl) for 1 hour at R/T and then incubated with anti-DIG antibody (Roche; 1:5000) overnight ...
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bioRxiv - Genetics 2019Quote: Digoxigenin (DIG)-labelled anti-sense myl7 and mef2cb [25] riboprobes were synthesized from linearized pGEM-T easy and pCMV-SPORT6.1 vectors respectively using the DIG RNA labeling mix (Roche) and the T7 RNA polymerase (Roche) ...
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bioRxiv - Immunology 2021Quote: ... Cells producing SpyC-Dps were re-suspended in T-buffer1 (30 mM Tris, 250 mM NaCl, pH 8.0) with one tablet of Complete Protease Inhibitors (Roche) per 10 g cells wet weight ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were washed 2x in PBS + 0,1% Tween-20 (PBS-T) followed by 15 min permeabilization in 1- μg/ml proteinase K (Roche) and 3 washes in PBS-T ...
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bioRxiv - Developmental Biology 2020Quote: ... The amplified sequence was cloned into a pGEM-T Easy Vector before digestion and DIG RNA labeling (Roche #11277073910).
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bioRxiv - Immunology 2020Quote: ... These were identified by immunohistochemical staining (IHC) with antibodies against T cells: CD3 (Roche Cat # 790-4341, pre-diluted), CD4 (Roche ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... These cells were cultured with healthy donor T cells (E:T ratio of 5:1) and different concentrations of Mosunetuzumab (Genentech, Roche): 0 ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA molecules were enriched from 500 ng of total RNA using poly-T oligo-attached magnetic beads (Kapa Biosystems). The first-strand cDNA was synthesized using a reverse transcriptase ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... the fasting glucose levels were measured at t=0 via tail vein blood using a glucometer (Accu-Check, Roche). For OGTT ...
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bioRxiv - Microbiology 2019Quote: ... after which they were centrifuged through a TE Select-D G-25 spin column (Roche Applied Science) to remove unincorporated [32P] dATP ...
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bioRxiv - Genomics 2020Quote: ... 25 μl of 2x KAPA Hifi HotStart Ready Mix (Kappa Biosystems, Roche Sequencing and Life Science, USA) and 10 μl of nuclease-free water ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mm EDTA in ddH2O) supplemented with 1:25 cOmplete Protease Inhibitor Cocktail (Roche Diagnostic, catalog #11697498001) and 1:200 PMSF ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed in 25 µl reaction mix containing 2x KAPA HIFI HotStart Ready-mix (Roche #07958935001), 10µM primers and 10 ng of gDNA template from stool or tissue under the following conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 µl anti-CENP-ACnp1 (sheep in-house antiserum) and 25 µl Protein-G-Agarose beads (Roche) or 2 µl of anti-GFP (A11122 ...
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bioRxiv - Genetics 2020Quote: ... we combined 800 ng of plasmid library with 25 µL 2x Kapa library amplification mix (Roche, #KK2702), 1 µL forward primer (10 µM ...
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bioRxiv - Plant Biology 2020Quote: ... PCR was performed in 25 μl reactions containing 12.5 μl KAPA HiFi Hotstart ReadyMix [2X] (Kapa biosystems), 1.25 μl 5 μM mPing fusion primer (Supplemental Table 3) ...
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bioRxiv - Cell Biology 2022Quote: ... 25 mM potassium chloride and 1 mM EDTA) in the presence of a protease inhibitor cocktail (Roche). Cell lysis was performed using mechanical homogenisation with constant incubation of the samples on ice ...
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bioRxiv - Cell Biology 2019Quote: ... the tryptic digests were cleaved by chymotrypsin (5 ng/μl, sequencing grade, Roche, in 25 mM AB) for 2 hours at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 ng of dsDNA was then used as input for the KAPA Hyper Prep Kit (KK8504, Roche) and ligated to KAPA single indexed adapters Set B (KK8702 ...
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bioRxiv - Cancer Biology 2021Quote: ... 21.5 μl of the DNA samples were mixed with 25 μl KAPA HiFi HotStart ReadyMix (KAPA Biosystems), 1 μl PE1.0 primer (100 μM ...
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bioRxiv - Microbiology 2023Quote: ... and the cell suspension were digested in HBSS containg 25 μg /ml of DNAse I (#11284932001, Roche) and 50 μg/ml Liberase (#5401119001 ...
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bioRxiv - Plant Biology 2023Quote: ... Each reaction was performed in 25 μL total volume with 12.5 μL KAPA HiFi HotStart ReadyMix (Roche), one μL from 10 μM of each primer ...
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bioRxiv - Immunology 2023Quote: ... minced whole spleens were digested in basal RPMI 1640 supplemented with 25 ug/ml Liberase TM (Roche) and 25 ug/ml DNase I (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Triplicate qPCR reactions of the Telomeric (T) product and the Single copy gene (S) (HBB) were amplified using a Roche LightCycler 480 II (Roche) using the following programs ...
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bioRxiv - Cell Biology 2020Quote: ... bovine serum albumin in TBS-T and incubated for 16 h at 4°C: mouse anti-GFP (1:1000) (Roche), sheep anti-CK1α (1:100 ...
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bioRxiv - Developmental Biology 2021Quote: ... Digxigenin (DIG)-labeled sense and antisense probes were performed from the linearized pGEM-T-easy plasmids using the DIG RNA Labeling Kit (Roche).
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bioRxiv - Neuroscience 2022Quote: ... At DIV 17 cells were washed with ice-cold PBS and immediately extracted with ice-cold T-PER protein extraction buffer (Pierce) supplemented with inhibitors for proteases and phosphatases (Roche). The lysates were centrifuged at 15000 g for 15 minutes at 4°C and the supernatants were used for further analysis.
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bioRxiv - Genetics 2022Quote: ... RNA-seq libraries of template molecules suitable for high throughput sequencing were established from 300ng of total RNA using the KAPA RNA HyperPrep Kit after a first step of purification using poly-T oligo-attached magnetic beads (Roche). Libraries were sequenced on the Illumina Hiseq 4000 sequencer as paired-end 100 base reads ...
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bioRxiv - Neuroscience 2022Quote: ... homogenized on ice in 5 volumes (w/v) of T-per extraction buffer complemented with protease inhibitor tablets (Complete Mini Protease Inhibitor Tablets, Roche) and phosphatase inhibitor cocktail tablets (phosSTOP ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were homogenized on ice in 5 volumes (w/v) of T-per extraction buffer (Pierce, USA) complemented with protease inhibitor tablets (Complete Mini Protease Inhibitor Tablets, Roche) and phosphatase inhibitor cocktail tablets (phosSTOP ...
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bioRxiv - Bioengineering 2021Quote: ... P14 CD8+ T cells were activated for 24 h as described above and resuspended in T cell media + 30 U/ml rhIL-2 (Roche) at 2 × 106 cell/ml ...
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bioRxiv - Biophysics 2021Quote: ... including 200 μM final concentration of each dNTP (G,C,T,A) and 10 µM Bio-16-dUTP or Dig-11-dUTP (all from Roche). Labelled handles specifically bind either to a glass surface covered with anti-digoxigenins or to superparamagnetic beads covered with streptavidin ...
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bioRxiv - Physiology 2020Quote: 1 μg RNA was reverse transcribed into cDNA with Oligo(d)T primers using the Transcriptor First Strand cDNA Synthesis kit (Roche). The qRT-PCR was performed with the LightCycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Immunology 2022Quote: ... the slides were washed in TBS/T 3 times and then incubated with secondary antibodies (2 µg/ml) and DAPI (1 µg/ml, Roche) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T-cells were plated in 200ul of medium (RPMI, 10% human serum) containing IL-2 (Roche, 10 IU/mL) and IL-7 (Peprotech ...
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bioRxiv - Biochemistry 2023Quote: ... cell pellets were resuspended in HR buffer (50 mM Tris-HCl, 5 mM MgCl2, 250 mM sucrose, 2 mM TCEP and protease inhibitor t (Roche), pH 7.4) ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked in 5% low-fat milk in TBS-T for 2 hours and probed with 1:5000-diluted mouse anti-GFP (Roche) or rabbit anti-ATG8 (Agrisera ...
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bioRxiv - Bioengineering 2023Quote: ... where a small opening (≈ 0.5 mm) was made using Accu-Check® Safe-T-Pro Plus lancing device (Roche AG) pricking needle ...
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bioRxiv - Cell Biology 2022Quote: ... All siRNA were transfected or co-transfected with emerin plasmids at 25 nM using X-tremeGENE HP (Roche). When associated with lentiviral expression of emerin ...
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bioRxiv - Molecular Biology 2020Quote: ... The 1st round PCR was performed by adding 25 μl 2x KAPA HiFi HotStart Ready Mix (KAPA biosystems) with primers of NEB Oligos kit ...
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bioRxiv - Neuroscience 2020Quote: ... 25 mM Tris-HCl pH 7.6) containing phosphatase and protease inhibitors (PhosSTOP™, cOmplete™; both from Roche). Protein concentration was determined with the Pierce™ BCA Protein Assay Kit (Pierce™ ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting pure nuclear pellet was first resuspended with 70 μL low salt buffer (20 mM HEPES, pH 7.6, 1.5 mM MgCl2, 10 mM KCl, 25% glycerol, protease inhibitor (Roche)) and 2.5 μL benzonase (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... purified GST-RomA was incubated with equal amounts of HIS6-LphD or HIS6-LphD Y392F in protein binding buffer (25 mM Tris pH 8.0, 140 mM NaCl, 3 mM KCl, 0.1% NP40 with protease inhibitors (ROCHE)) overnight at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... A plasmid (25 ng reporter, 100 ng effector) was transfected with 0.4 μl FuGENE HD (Roche, Basel, Switzerland) per well at a cell confluence level of 50-70% ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells are pelleted and resuspended in 1 mL of Lysis Buffer (200 mM NaCl, 25 mM HEPES, 1 mM MgCl2, 0.2 mM EDTA, 0.5% NP-40, 1X Roche complete inhibitor ...