Labshake search
Citations for Roche :
101 - 150 of 156 citations for Streptavidin FITC Conjugate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... Tissue was incubated with the probe overnight and Arc positive cells were detected with anti-digoxigenin-HRP conjugate (Roche Applied Science Ref # ...
-
bioRxiv - Biochemistry 2023Quote: ... the Dig at the end of the DNA-bead conjugate was first blocked with anti-digoxigenin Fab fragment (Roche) in a ratio of 1:2 respectively in Buffer C with 7 mM MgCl2 ...
-
bioRxiv - Plant Biology 2019Quote: ... Expression of prey fusion proteins was confirmed by immunoblotting with anti-HA-HRP conjugate (clone 3F10, Roche, Indianapolis, IN, USA). Proteins were detected using chemiluminescent Pierce ECL 2 substrate (Thermo Fisher Scientific ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Labelling efficiencies were tested by dot blotting with anti-digoxigenin alkaline phosphatase conjugate and CSPD chemiluminescence substrate for alkaline phosphatase from DIG High Prime DNA Labelling and Detection Starter Kit II (Roche) according to the manufacturer`s protocol ...
-
bioRxiv - Developmental Biology 2021Quote: ... brn3a1 and brn3a2(l) transcripts were visualized with anti digoxigenin-alkaline phosphatase conjugates and BCIP/NBT or BM purple substrates (Roche). For two color WISH krx-20 (Oxytoby and Jowett ...
-
bioRxiv - Biophysics 2021Quote: ... at one end through biotin-streptavidin interactions and to the anti-digoxigenin (Roche, Basel, Switzerland) coated coverglass surface at the other end through digoxigenin-antibody interactions ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plates were then incubated with streptavidin-conjugated horseradish peroxidase (HRP) (Roche Molecular Biochemicals, #1089153) at 1 U/ml for 1 h at 4°C and further washed three times with TPBS in 30 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin- or FITC-labeled antisense probes were synthesized using an RNA Labeling Kit (SP6/T7; Roche). The staining was revealed with NBT/BCIP or INT/BCIP substrate (Roche) ...
-
bioRxiv - Genomics 2022Quote: ... and digoxigenin probes were detected with 4μg/ml anti-digoxigenin conjugated to FITC (fluorescein isothiocyanate, Roche). Slides were simultaneously counterstained and mounted by applying antifade mixture [6μl DAPI (4’ ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Hybridization signals were detected by Anti-Digoxigenin-FITC (Roche; dilution 1:10 in 0.5% BSA/PBS) and Streptavidin-Cy3 (Invitrogen Life Technologies ...
-
bioRxiv - Neuroscience 2023Quote: ... Sst sense and antisense probes were transcribed using a DIG or FITC RNA labeling kit (Roche) and purified with RNA Clean & Concentrator (Zymo Research) ...
-
bioRxiv - Developmental Biology 2023Quote: ... FITC-labelled probes were detected first by incubating with sheep Anti-Fluorescein-POD Fab fragments (Roche) at a 1:50 dilution ...
-
bioRxiv - Neuroscience 2024Quote: ... Larvae were incubated overnight at 4°C in horseradish peroxidase-coupled anti-FITC antiserum (11426346910, Roche) diluted 1:500 in blocking solution ...
-
bioRxiv - Neuroscience 2024Quote: ... Tissues were incubated overnight at 4°C in alkaline phosphatase-coupled anti-FITC antiserum (11426338910, Roche) diluted 1:5000 in blocking buffer ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... and subsequently captured with streptavidin-coated magnetic capture beads using the NimbleGen SeqCap EZ Kits (Roche NimbleGen Inc ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cytofluorimetric recordings of phosphatidylserine exposure on the cell surface (increased staining of FITC-conjugated Annexin-V; Roche) and loss of plasma membrane integrity (7-AAD staining ...
-
bioRxiv - Molecular Biology 2021Quote: ... which was labelled with digoxygenin (DIG) and detected with sheep anti-DIG FITC (11207741910, Roche, RRID: AB_514498) and rabbit anti-sheep FITC antibodies (FI-6000 ...
-
bioRxiv - Cell Biology 2023Quote: ... RNA probes were transcribed and labeled with either FITC (fluorescein isothiocyanate) or DIG (digoxigenin) NTPs (Roche Cat#).
-
bioRxiv - Neuroscience 2023Quote: ... Tissue was incubated in the generated probes overnight and labeled with an anti–digoxigenin-HRP conjugate or anti-fluorescein-(Ref#: 11207733910, Lot # 43500600 & REF#11426346910; LOT#45220020; Roche Applied Science). Fluorescein (Fluorescein Direct FISH ...
-
bioRxiv - Biochemistry 2021Quote: ... the presynaptic filament was formed by incubating 5 μl of streptavidin-coated magnetic resin (Roche Molecular Biochemicals) with 5’-biotinylated 80-mer ssDNA oligonucleotide (5 μM ...
-
bioRxiv - Biochemistry 2019Quote: ... Bound biotin peptides were then detected by incubating with 1 U/ml of HRP-conjugated streptavidin (Roche) on ice for 45 min in PBS/0.1% Triton X-100 ...
-
bioRxiv - Genomics 2022Quote: ... Slides were washed at 42°C in 0.1xSSC and hybridization sites were detected with anti-digoxigenin-FITC (2µg/ml; Roche) and Streptavidin-Alexa594 (1µg/ml ...
-
bioRxiv - Developmental Biology 2019Quote: ... After a series of washes the probes were detected using FITC conjugated anti-Digoxigenin antibody (1:20, Roche) amplified with anti-sheep Alexa Fluor 488 (1:100 ...
-
bioRxiv - Genetics 2023Quote: ... ssRNA FISH probes were labelled using FITC-UTP and purified through Mini Quick Spin RNA columns (Roche, #11814427001). Labelled double-stranded and ssRNA FISH probes were then precipitated in presence of 0.3M sodium acetate (Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... the brain sections were incubated with peroxidase (POD)-conjugated anti-FITC antibody (Roche Applied Science, Germany; 1:2,000) or POD-conjugated anti-Digoxigenin antibody (Roche Applied Science ...
-
bioRxiv - Neuroscience 2020Quote: ... and then incubated for one hour in donkey anti-mouse FITC antibody (1:200) in 0.3% Triton-X100 supplemented with 1X blocking buffer (Roche). After washing with PBS for 5 min ...
-
bioRxiv - Neuroscience 2022Quote: ... Digoxigenin (DIG)-labeled or fluorescein (FITC)-labeled riboprobes were synthesized with T7 or T3 or SP6 RNA polymerase (Roche) from the linearized plasmids with DIG or FITC RNA labeling mix (Roche) ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2011) or Tyramide Signal Amplification (TSA) after incubation with anti-digoxigenin-AP or anti-FITC-POD (Roche, 1:300) antibodies ...
-
bioRxiv - Neuroscience 2020Quote: ... and then incubated for in donkey anti-mouse FITC antibody diluted in 10 X blocking buffer (1:100; Roche) + 0.3 % Triton-X100 for 1 h ...
-
bioRxiv - Neuroscience 2022Quote: ... Antisense and sense single-strand riboprobes were transcribed in vitro using DIG or FITC RNA labeling kit (Roche Diagnostics). cRNA probes for Gad155 and Slc17a756 were used.
-
bioRxiv - Developmental Biology 2020Quote: ... gbx1 and pax6a digoxigenin and FITC antisense probes were generated from linearized plasmids using an RNA labelling and detection kit (Roche)87 ...
-
bioRxiv - Neuroscience 2019Quote: ... serine/threonine kinase A-Raf (Araf) were in vitro transcribed from template cDNA using T7/T3/SP6 RNA polymerase (DIG/FITC RNA labeling kit, Roche) and purified with RNA Clean & Concentrator (Zymo Research) ...
-
bioRxiv - Physiology 2019Quote: ... Tissue slices were incubated with hybridization probes for 18 h at 55°C and then incubated with sheep anti-DIG and anti-FITC antibodies conjugated with peroxidase (POD; 1:250; Roche) for probe labeling using the InSituPro VSI robot (Intavis ...
-
bioRxiv - Developmental Biology 2019Quote: ... RNA probes were generated using PCR with T7 promoter sequence linkers and subsequently transcribed [DIG or FITC labeled] using T7 polymerase (Roche). Primer sequences are all found in Table 3 ...
-
bioRxiv - Neuroscience 2021Quote: ... Probes were detected by horseradish peroxidase (POD)-conjugated antibodies (anti-FITC-POD at 1/250 dilution, Roche; anti-DIG-POD at 1/500 dilution, Roche). Signals were amplified by biotin-conjugated tyramide (PerkinElmer ...
-
bioRxiv - Developmental Biology 2020Quote: ... 500 ng DNA was subjected to in vitro transcription with T3 polymerase and DIG- or FITC-labeled ribonucleotides (All Roche) for 2 hours at 37°C ...
-
bioRxiv - Developmental Biology 2023Quote: ... Resulting amplicons were used as templates for the synthesis of either digoxigenin (DIG) or fluorescein (FITC)-labelled anti-sense probes by in vitro transcription using T7 or T3 polymerases (Roche) and DIG or FITC RNA labelling mixes (Roche).
-
bioRxiv - Synthetic Biology 2021Quote: ... the efficiency of biotinylation was checked in a Western blot revealed with POD-anti-biotin (horseradish peroxidase conjugated with Streptavidin, Roche).
-
bioRxiv - Pathology 2021Quote: ... Tissue sections were then blocked with blocking solution buffer II (containing 0.1% Triton X-100 and 2% normal sheep serum) at room temperature for 30 min before covering with anti-DIG alkaline phosphatase conjugate anti-digoxigenin antibody (Roche, diluted 1:500 in buffer II) for 1 h at 45°C ...
-
bioRxiv - Plant Biology 2020Quote: ... The sites of hybridization of digoxigenin- and biotin-labeled probes were detected using anti-digoxigenin-FITC (Roche Applied Science, Penzberg, Germany) and streptavidin-Cy3 (ThermoFisher Scientific/Invitrogen ...
-
bioRxiv - Plant Biology 2020Quote: ... were labelled by PCR in the presence of digoxygenin-11-dUTP detected by antidigoxigenin-fluorescein isothiocyanate (FITC; both from Roche Diagnostics) and biotin-16-dUTP (Roche Diagnostics ...
-
bioRxiv - Genetics 2019Quote: ... Coverslips were blocked in 3% BSA (wt/vol) and digoxigenin was detected with sheep anti-digoxigenin FITC 1/50 (Roche, 11207741910) followed by rabbit anti–sheep FITC 1/100 (Vector Laboratories ...
-
bioRxiv - Neuroscience 2022Quote: ... for 18 hours at 55°C and incubated with different combinations of anti-fluorescein isothiocyanate (FITC)- and anti-digoxigenin (DIG)-conjugated antibodies (Roche Diagnostics), followed by tetramethylrhodamine (TAMRA)-(Thermofisher ...
-
bioRxiv - Plant Biology 2022Quote: ... Hybridization signals of probes labelled with digoxigenin-11-dUTP and biotin-16-dUTP were detected with antidigoxigenin conjugated to fluorescein isothiocyanate (FITC; Roche Diagnostics) and streptavidin conjugated to Alexa 594 or Alexa 647 (Life Technologies-Molecular Probes) ...
-
bioRxiv - Genomics 2023Quote: ... was indirectly labeled by PCR in the presence of digoxigenin-11-dUTP detected by antidigoxigenin-fluorescein isothiocyanate (FITC; both from Roche Diagnostics). The remaining satDNAs (‘pTS5’ ...
-
bioRxiv - Neuroscience 2023Quote: ... and Vglut3 and fluorescein (FITC)-labeled mRNA against DBH using reverse-transcribed mouse cDNA as a template and an RNA DIG or FITC-labeling kits from Roche (Sigma). Primer and probe sequences for the Vglut1 ...
-
bioRxiv - Neuroscience 2023Quote: ... and incubated at room temperature for 30 minutes with HRP-labeled sheep anti-FITC antibody (1/500 in TNB, Roche/Sigma). Following washes in TNT ...
-
bioRxiv - Cancer Biology 2024Quote: ... The consecutive IF detection system for the above-mentioned sequence of antibodies was developed by using the Discovery FITC kit (catalog no. 760-232, Roche-Ventana), Discovery Red 610 (catalog no ...
-
bioRxiv - Molecular Biology 2022Quote: ... Biotin-labeled LPA1-HA was detected using streptavidin beads (Pierce) and conjugated anti-HA antibody (3F10 from Roche Diagnostics; 1:1000).
-
bioRxiv - Biochemistry 2019Quote: ... and the resulting duplex was captured on 3 mg streptavidin-coated magnetic beads (Roche, rotation for 2 h at 37°C). After extraction with phenol-chloroform and precipitation with ethanol ...