Labshake search
Citations for Roche :
201 - 250 of 304 citations for Streptavidin AP Conjugate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... Signal was then detected by use of: “Anti-Digoxigenin-AP Fab fragments” (Roche, Basel, CH), “CDP-Star” solution (Roche ...
-
bioRxiv - Developmental Biology 2023Quote: ... and for whole mount in situ hybridization (WISH) we used anti-digoxigenin-AP antibody (Roche cat ...
-
bioRxiv - Developmental Biology 2024Quote: ... RBR buffer was removed and replaced with 1:5000 anti-fluorscein-AP antibody (Roche #11681460001) in RBR buffer and incubated on the 4C orbital shaker overnight ...
-
bioRxiv - Neuroscience 2023Quote: Incorporated DIG was detected with anti-DIG Fab fragments conjugated to alkaline phosphatase (AP, Roche). This conjugate was preadsorbed with octopus embryo powder in 5% DIG buffer in TBST for at least one hour ...
-
bioRxiv - Developmental Biology 2024Quote: ... the sections were incubated with sheep anti-digoxigenin-alkaline phosphatase (AP) antibody (1:3000, Roche) for 90 min at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... Sections were incubated overnight at 4°C with Anti-Digoxigenin-AP antibody (1:2000, Roche) in blocking solution ...
-
bioRxiv - Genetics 2019Quote: ... the streptavidin magnetic beads were subjected to PCR amplification with HiFi Kapa (Kapa Biosystems) for 12 cycles ...
-
bioRxiv - Microbiology 2022Quote: ... detection of bound conjugate was achieved by the use of an enhanced chemiluminescence assay (ECL) kit (Roche Applied Science). Images were acquired using a BIO-RAD ChemiDoc imaging system.
-
bioRxiv - Genomics 2021Quote: ... Samples were then incubated overnight at 4°C with a rabbit anti-DIG HRP-conjugate antibody (1:500, Roche). Then ...
-
bioRxiv - Neuroscience 2022Quote: ... Tissue was incubated with the probe overnight and Arc positive cells were detected with anti-digoxigenin-HRP conjugate (Roche Applied Science Ref # ...
-
bioRxiv - Biochemistry 2023Quote: ... the Dig at the end of the DNA-bead conjugate was first blocked with anti-digoxigenin Fab fragment (Roche) in a ratio of 1:2 respectively in Buffer C with 7 mM MgCl2 ...
-
bioRxiv - Developmental Biology 2020Quote: ... the embryos were washed with PBST and incubated in BM Purple AP Substrate (Roche, Basel, Switzerland) at room temperature in the dark ...
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with alkaline phosphatase (AP)-conjugated anti-DIG antibody (1:1000, 11093274910; Roche) at 4°C for two days ...
-
bioRxiv - Microbiology 2020Quote: ... before electrophoretic transfer to a nylon membrane and IB using anti-Digoxigenin-AP Fab fragments (Roche). Visualisation of bands used CDP-Star chemiluminescence reagents (Roche).
-
bioRxiv - Neuroscience 2022Quote: ... for 1 hr at RT and incubated overnight with anti-Digoxigenin-AP (1:4000, Roche 11093274910) in Blocking Buffer ...
-
bioRxiv - Developmental Biology 2022Quote: ... Visualization of the labeled probe was done using anti-DIG-AP (alkaline phosphatase) antibodies (Roche Biochemicals) followed by histochemical enzymatic staining reagents (Roche Biochemicals) ...
-
bioRxiv - Developmental Biology 2022Quote: ... The samples were incubated with alkaline phosphatase (AP)-conjugated anti-DIG antibodies (1:2000, Roche, 11175041910) in 1% blocking buffer for 4 h at room temperature ...
-
bioRxiv - Developmental Biology 2019Quote: ... Probes were detected by over-night incubation with anti-Digoxigenin-AP Fab fragments (1:5000) (Roche) and stained with standard Nitro Blue Tetrazolium (NBT ...
-
bioRxiv - Molecular Biology 2022Quote: ... at 42 °C and detection was performed by chemiluminescence using anti-Digoxigenin-AP Fab fragments (Roche) and CDP-Star chemiluminescent substrate (Roche ...
-
bioRxiv - Plant Biology 2022Quote: ... and incubated for 30 mins in 1:5000 Anti-Digoxigenin- AP Fab Fragment antibodies (Roche; 11093274910). After washing the membrane twice in buffer 1 for 15 mins ...
-
bioRxiv - Neuroscience 2022Quote: ... then incubated for four hours at RT in AP-conjugated anti-DIG Fab fragments (Roche, 11093274910) diluted 1:2000 in 2% DIG blocking reagent ...
-
bioRxiv - Developmental Biology 2023Quote: ... the embryos were washed with PBST and incubated in BM Purple AP Substrate (Roche, Basel, Switzerland) at room temperature in the dark ...
-
bioRxiv - Physiology 2024Quote: ... and incubated overnight with the alkaline phosphatase (AP) coupled anti-digoxigenin or anti-fluorescein antibody (Roche) at respectively 1/5000 or 1/1000 in blocking buffer ...
-
bioRxiv - Plant Biology 2019Quote: ... Expression of prey fusion proteins was confirmed by immunoblotting with anti-HA-HRP conjugate (clone 3F10, Roche, Indianapolis, IN, USA). Proteins were detected using chemiluminescent Pierce ECL 2 substrate (Thermo Fisher Scientific ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Labelling efficiencies were tested by dot blotting with anti-digoxigenin alkaline phosphatase conjugate and CSPD chemiluminescence substrate for alkaline phosphatase from DIG High Prime DNA Labelling and Detection Starter Kit II (Roche) according to the manufacturer`s protocol ...
-
bioRxiv - Developmental Biology 2021Quote: ... brn3a1 and brn3a2(l) transcripts were visualized with anti digoxigenin-alkaline phosphatase conjugates and BCIP/NBT or BM purple substrates (Roche). For two color WISH krx-20 (Oxytoby and Jowett ...
-
bioRxiv - Developmental Biology 2023Quote: ... samples were blocked for 30 minutes in 1% Roche Western Blocking Reagent prior to incubating overnight at 4°C with either anti-FITC with horseradish peroxidase conjugate (Roche) at a 1:2000 concentration or anti-DIG-with horseradish peroxidase conjugate (Roche ...
-
bioRxiv - Biophysics 2021Quote: ... at one end through biotin-streptavidin interactions and to the anti-digoxigenin (Roche, Basel, Switzerland) coated coverglass surface at the other end through digoxigenin-antibody interactions ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plates were then incubated with streptavidin-conjugated horseradish peroxidase (HRP) (Roche Molecular Biochemicals, #1089153) at 1 U/ml for 1 h at 4°C and further washed three times with TPBS in 30 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... Signals of transcripts were detected using Anti-DIG-AP (alkaline phosphatase) and Fab fragments from sheep (Roche) and developed in Fast Red TR and naphthol-AS-MX-phosphate in 0.1 M Tris-HCl pH 8.2 (tablet set ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... Following hybridization, the sections were treated with secondary antibody anti-dig-ap (1:1000, 11093274910, Merk (Roche), UK) ...
-
bioRxiv - Developmental Biology 2022Quote: ... The embryos were then transferred to TBST containing 10% HISS and anti-DIG-AP antibody (Roche, 11093274910) at a dilution of 1:2000 overnight at 4C with constant rocking ...
-
bioRxiv - Physiology 2019Quote: ... at RT followed by incubation with mouse anti-DIG alkaline phosphatase (AP) antibody (1:2000) (#11093274910, Roche) in Roche blockingbuffer (1:10 in PBT ...
-
bioRxiv - Developmental Biology 2022Quote: ... slides were incubated with Blocking buffer II containing 0.1% Anti-DIG-AP fab anti body (Roche, 11093274910) overnight at 4°C ...
-
Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... For NBT/BCIP staining sections were incubated overnight with sheep anti-DIG-AP (1:1000, Roche 11093274910). After washing staining was performed using NBT/BCIP in NTMT until satisfactory intensity ...
-
bioRxiv - Physiology 2023Quote: ... sections were incubated for 3 hours with Anti-Digoxigenin-AP Fab fragments (dilution 1:3000) (Roche, 11093274910) at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... Detection with anti-digoxigenin antibody conjugated with alkaline phosphatase (Anti-Digoxigenin-AP Fab fragments, 11093274910, Roche, Switzerland) and activation with the chemiluminescent substrate CDP-Star (11759051001 ...
-
bioRxiv - Developmental Biology 2024Quote: ... the antibody incubation was performed with 1:5000 diluted Anti-Dig-AP antibody (Roche, Cat. No. 11093274910) at 4°C overnight ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... and subsequently captured with streptavidin-coated magnetic capture beads using the NimbleGen SeqCap EZ Kits (Roche NimbleGen Inc ...
-
bioRxiv - Plant Biology 2021Quote: ... and incubated at room-temperature with a 1:500 dilution of anti-Dioxigenin-AP Fab fragments (Roche, #11093274910) in 1% BSA/0.3 % Triton-X100/TBS for 1hour ...
-
bioRxiv - Neuroscience 2019Quote: ... In situ hybridization signals were detected with sheep anti-digoxigenin-AP Fab fragments (1:10,000; Roche Diagnostics, Germany). The color staining was carried out with chromogen substrates (nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate ...
-
bioRxiv - Microbiology 2021Quote: ... The terminal phosphate of the pGpG product is cleaved by the coupling enzyme AP (20 U/µl, Roche), and the phosphate concentration is determined from the fluorescence increase through binding of phosphate to the phosphate sensor (0.5 µM ...
-
bioRxiv - Neuroscience 2020Quote: ... Detection of hybridized probes was performed with anti-DIG antibodies AP fragments (Roche, Basel Switzerland; dilution 1:1000) overnight at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... In situ hybridization signals were detected with sheep anti-digoxigenin-AP Fab fragments (1:10000; Roche Diagnostics, Germany) conjugated with alkaline phosphatase ...
-
bioRxiv - Developmental Biology 2022Quote: ... blocked in TBTX/BSA/Sheep serum and then treated overnight with anti-DIG-AP antibody (1:2000; Roche). Following extensive washing in TBTX ...
-
bioRxiv - Developmental Biology 2022Quote: ... 1 % blocking reagent before incubation in a blocking solution with anti-DIG alkaline phosphatase (AP) conjugated antibody (Roche) diluted 1 ...
-
bioRxiv - Plant Biology 2022Quote: ... Hybridized probes were detected by anti-DIG antibodies conjugated with alkaline phosphatase enzyme (anti-DIG-AP) (Roche, Germany). Photographs were captured using an Olympus BX51 compound microscope using a DP74 Olympus camera.
-
bioRxiv - Plant Biology 2024Quote: ... Immunodetection was performed using an anti-DIG antibody coupled to alkaline phosphatase (Anti-Digoxygenin-AP, Fab fragments, Roche), whose activity was detected by the chromogenic method using NBT/BCIP (Roche) ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissue was incubated in the generated probes overnight and labeled with an anti–digoxigenin-HRP conjugate or anti-fluorescein-(Ref#: 11207733910, Lot # 43500600 & REF#11426346910; LOT#45220020; Roche Applied Science). Fluorescein (Fluorescein Direct FISH ...
-
bioRxiv - Biochemistry 2021Quote: ... the presynaptic filament was formed by incubating 5 μl of streptavidin-coated magnetic resin (Roche Molecular Biochemicals) with 5’-biotinylated 80-mer ssDNA oligonucleotide (5 μM ...