Labshake search
Citations for Roche :
651 - 700 of 5092 citations for Starch Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... The level of secreted prostate specific antigen (PSA) and T3 in the growth medium was determined using a validated Elecsys electrochemiluminescence immune assay (Roche, Rotkreuz, Switzerland) on a Cobas e601system (Roche ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell protein lysates were prepared by lysing cells in Radioimmunoprecipitation assay buffer (RIPA) supplemented with cOmplete Protease inhibitor (Roche, Basel, Switzerland) and PhosphStop (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... The thermofluor assay and calculation of melting temperatures were performed on a LightCycler® 480 II using LightCycler® 480 Software (Roche).
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bioRxiv - Cancer Biology 2022Quote: For antibody incubation 5 µl of the DigiWest Bead mixes were added to 50 µl assay buffer (Blocking Reagent for ELISA (Roche, Rotkreuz, Switzerland) supplemented with 0.2% milk powder ...
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bioRxiv - Epidemiology 2020Quote: ... plasmids or DNA samples (Table 1) by real-time TaqMan PCRs assays on a LightCycler® 480 (LC480) (Roche Applied Science, Germany). Real-time PCR assays were performed with LightCycler® 480 Probe Master Mix 1× (Roche Applied Science ...
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bioRxiv - Physiology 2019Quote: Quantitative real time PCR (qPCR) was performed with primer sets (Supplemental Table 1) designed using Roche Universal Probe Library Assay Design Center (Roche Diagnostics, UK) in conjunction with corresponding FAM-labelled probes ...
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bioRxiv - Pathology 2020Quote: ... The specific pairs of primers used for the real-time PCR are listed in Supplementary Table 4 (designed by Universal ProbeLibrary Assay Design Center tool from Roche, Indianapolis, IN). The real-time PCR was performed with FastStart Universal Probe Master mix ...
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bioRxiv - Biochemistry 2021Quote: ... washed twice with PBS and subsequently resuspended in assay buffer (20 mM HEPES pH 7.5, 150 mM NaCl, and 0.1% Triton X-100 supplemented with Roche cOmplete Protease Inhibitor Cocktail). The cells were lysed by sonication and cleared by centrifugation for 5 min at 14’000 rpm and 4°C ...
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bioRxiv - Cancer Biology 2022Quote: Whole-cell protein lysates were prepared by lysing cells in Radioimmunoprecipitation assay buffer (RIPA) supplemented with cOmplete Protease inhibitor (Roche, Basel, Switzerland) and PhosphStop (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... Respiratory viruses were detected from specimens by real-time PCR and reverse transcription (RT)-PCR assays with LightCycler instruments (Roche, Basel, Switzerland) as previously described (22 ...
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bioRxiv - Microbiology 2022Quote: ... The viral stocks were quantified by measuring the reverse transcriptase (RT) activity using either a qPCR-based product-enhanced RT (PERT) assay68 or a colorimetric RT assay (Roche Life Sciences).
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bioRxiv - Molecular Biology 2023Quote: We designed a multiplex quantitative PCR (qPCR) with four parallel assays to be run on the Roche 480 light cycler (Roche, Basel, Switzerland). Compatible fluorescent dyes (FAM ...
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bioRxiv - Neuroscience 2023Quote: ... alpha synuclein and NfL) were measured in both cohorts using Elecsys assays in accordance with the manufacturer’s instructions (Roche Diagnostics International Ltd).52 CSF analyses were performed by technicians blinded to all clinical and imaging data ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Pooled library quality was assessed on the Fragment Analyzer and molarity determined using a qPCR-based Library Quantification assay (Roche, Pleasanton, CA).
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bioRxiv - Zoology 2023Quote: ... which were quality checked on an Agilent 4200 Tapestation and quantitated using a Lightcycler qPCR assay (Roche Life Science, St. Louis, MO). Libraries were paired-end sequenced (2 x 150 bp ...
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bioRxiv - Neuroscience 2024Quote: ... neurofilament light (NfL) and sTREM2 were measured as part of the NeuroToolKit assay panel in accordance with the manufacturer’s instructions (Roche Diagnostics International Ltd)(64) ...
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bioRxiv - Plant Biology 2019Quote: ... one with the Illumina Genomic DNA Sample Prep kit and one with the Kapa Hyper kit (Kapa Biosystem, Roche). Three mate-pair libraries were constructed using Illumina Nextera Mate Pair Library Prep kit with insert sizes ranging between 2-4 kb ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were validated for quality on Agilent DNA1000 Kit and quantified with the qPCR NGS Library Quantification kit (Roche). The final libraries were equimolar pooled and sequenced using the High Output 1×75 kit on the Illumina NextSeq500 platform producing 75bp single-end reads ...
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bioRxiv - Developmental Biology 2022Quote: ... These were synthesised by in vitro transcription with DIG-RNA labelling Kit or Fluorescein-RNA labelling kit (Roche, Switzerland). The integrity of probes was determined by gel electrophoresis and storage in 50% RNAlater (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... Capture library concentrations were determined by qPCR with a KAPA qPCR kit (KAPA Library Quant Kit, Kapa Biosystems, USA) and pooled at the same concentration for sequencing ...
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bioRxiv - Genetics 2021Quote: ... Capture-C libraries were made using the Arima HiC kit (Arima Genomics) and the KAPA HyperPrep kit (KAPA Biosystems) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The absence of kit/reagent contamination was verified in the High Pure Viral Nucleic Acid Kit (Roche Applied Science) and Illustra™ GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2023Quote: ... and the second-strand cDNA was synthesized using a KAPA Biosystems kit (Roche KAPA HiFi Hotstart PCR kit, KK2502) with VHgene-specific primers (No ...
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bioRxiv - Molecular Biology 2020Quote: ... targeting the HBV_circ_1 B site was labelled with DIG-11-dUTP by digoxin using a Dig high primer DNA labelling kit and detection starter kit II (Roche). Hepatic tissue paraffin sections of HCC patients were treated with dewaxing and then digested with protease K ...
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bioRxiv - Neuroscience 2021Quote: ... quantified using High Sensitivity DNA Kit run on Agilent 2100 Bioanalyzer and also KAPA Illumina library quantification kit (Roche, 7960140001) run on LightCycler 480 ...
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bioRxiv - Developmental Biology 2021Quote: Final RNA-seq libraries were quantified using the KAPA Library Quantification Kits for Illumina sequencing platforms (Roche, Kit code KK4824). 100bp paired-end sequencing reads (Illumina HiSeq2000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated from each sample using RNeasy Mini Kit (QUIAGEN) and cDNA was synthesized using Transcriptor First Strand cDNA Synthesis Kit (Roche). qPCR was performed in SYBR Green I Master Mix with sequence specific primers for genes of interest (Table 1. ...
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bioRxiv - Neuroscience 2021Quote: ... was used to capture lncRNAs in accordance with the SeqCap EZ Hybridization and Wash Kit and SeqCap EZ Accessory Kit (Roche). Captured lncRNA libraries were sequenced on an Illumina HiSeq 4000 platform with a read length of 150 bp x 2.
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bioRxiv - Cell Biology 2022Quote: ... genomic DNA was amplified using ResolveDNA Whole Genome Amplification Kit (BioSkryb Genomics) and then libraries were prepared for sequencing (Kapa Hyperplus Kit, Roche). All libraries were sequenced on either an Illumina MiniSeq or NextSeq instrument (Illumina ...
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bioRxiv - Pathology 2022Quote: Total RNA was purified from cells using a Tiangen Easy Fast RNA Extraction kit and reverse-transcribed using Transcriptor First Strand cDNA Synthesis kit (Roche). Expression of mtDNA encoded genes were determined by quantitative real-time RT-PCR using SYBR Green PCR Master Mix (Roche Applied Science) ...
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bioRxiv - Neuroscience 2020Quote: Tissue samples were homogenized and RNA was extracted using an RNAeasy Mini Kit (Quiagen, Hilden, Germany) and reverse transcribed using a Transcriptor First-Strand cDNA Synthesis kit (Roche Diagnostics ...
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bioRxiv - Synthetic Biology 2019Quote: ... purified by gel electrophoresis and extracted from the gel via a commercial kit (HighPure PCR Product Purification Kit, Roche, DE). Sample preparation (Illumina TruSeq amplicon library ...
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bioRxiv - Plant Biology 2019Quote: ... The blots were hybridized with probes that were labeled using PCR DIG Probe Synthesis Kit and hybridization was detected with DIG Luminescent Detection Kit following the manufacturer’s instructions (Roche, Switzerland).
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bioRxiv - Genomics 2021Quote: ... Both libraries were quality controlled using a Fragment analyzer NGS kit (AATI) and qPCR with the Kapa library quantification kit (Roche) prior to sequencing.
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bioRxiv - Genomics 2022Quote: ... whole transcriptome mRNA-enriched RNA-Seq libraries were constructed from cell lysates using a KAPA mRNA Capture Kit and mRNA HyperPrep Kit (Roche) as per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted the DNA with the MagAttract HMW DNA extraction kit and prepared short-inserted paired-end libraries for the whole genome with the KAPA HyperPrep kit (Roche) PCR-free protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 ug of total RNA was used for the construction of ribosome- depleted sequencing libraries using the KAPA RNA HyperPrep Kit with RiboErase kit (HMR, Roche) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2023Quote: ... anti-DIG antibody (Anti-Digoxigenin-AP Fab fragments) and Chemiluminescent detection with CSPD following the instructions of the kit (all products and kits from ROCHE).
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bioRxiv - Plant Biology 2023Quote: ... The resulting library was assessed with Fragment Analyzer (Advanced Analytical Technologies) using the Genomic DNA Kit DNF-467 and quantified with KAPA Library Quantification Kit (Roche). A single lane of HiSeq3000 was used for sequencing with 2×151 bp read length in presence of 1% PhiX (Illumina) ...
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BTK and MMP9 regulate NLRP3 inflammasome-dependent cytokine and NET responses in primary neutrophilsbioRxiv - Immunology 2024Quote: ... the amount of LDH released during the in vitro experiments were quantified using the kit Cytotoxicity Detection Kit (LDH) provided by Roche. Triton X-100 (Applichem ...
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bioRxiv - Cancer Biology 2024Quote: ... and DAB staining was detected using the UltraView Universal DAB Detection Kit or the OptiView DAB IHC Detection Kit (all from Roche). The following antibodies were used ...
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bioRxiv - Cell Biology 2020Quote: Mammalian cell lysates were prepared in radioimmuno-precipitation assay (RIPA) buffer supplemented with 1 tablet of EDTA-free protease inhibitor (Roche, Cat No. C762Q77) per 25ml buffer ...
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bioRxiv - Microbiology 2020Quote: Deidentified remnant patient samples that underwent routine clinical testing with the cobas SARS-CoV-2 assay on the 6800 platform (Roche Diagnostics, Indianapolis, IN) were used to evaluate the Xpert and ID Now assays ...
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bioRxiv - Bioengineering 2019Quote: ... The qPCR assay was performed on a Roche LightCycler® 480 Instrument II using LightCycler® 480 Probes Master (Roche Applied Science, Germany), UniWDVfw and UniWDVrv primers [33] and a TaqMan probe (6-FAM-AGGCGAAGAATGATTCACCCT-BHQ-1) ...
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bioRxiv - Plant Biology 2020Quote: ... Real-time quantitative PCR assays were performed using the KOD SYBR® qPCR Mix (TOYOBO) on the Light Cycler 96 system (Roche Diagnostics, Germany). ΔCt method was used for estimating transcript abundance ...
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bioRxiv - Microbiology 2022Quote: ... and thiosulfohydrolase (soxB) of sulfide oxidation were quantified by SYBR-Green based qPCR assays using a LightCycler 480 II (Roche Life Science, Switzerland). Published primer pairs and a newly designed primer set for soxB were applied (Tables S1 ...
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bioRxiv - Cell Biology 2023Quote: The spaced potassium assay has been carried out as described before (Piccioli and Littleton, 2014; Roche et al., 2002; Ataman et al., 2008). Briefly ...
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bioRxiv - Pathology 2023Quote: Sections were stained for CD8 and pan-cytokeratin (CK) in a dual chromogenic IHC assay on a Discovery ULTRA platform (Roche Tissue Diagnostics (RTD), Tucson ...
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bioRxiv - Zoology 2021Quote: ... quantified by qPCR Kapa Library quantification kit (Roche) and sequenced on an Illumina MiSeq (PE300 ...
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bioRxiv - Cell Biology 2020Quote: ... Signal was detected using LumiLight Plus Kit (Roche) on the Chemidoc MP imaging system (Biorad).