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Citations for Roche :
1 - 50 of 1095 citations for Sodium 40 wt. % dispersion in oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Whole cell extracts were prepared by lysing cells in RIPA buffer (50 mM Tris HCl pH 7.4/150 mM NaCl/0.5% sodium deoxycholate/0.1% sodium dodecyl sulfate/1% NP-40) containing cOmplete protease inhibitor cocktail (Roche), followed by quantification using Bradford assays ...
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bioRxiv - Microbiology 2023Quote: ... 0.5% NP-40 and 0.5% sodium deoxycholate) supplemented with a protease inhibitor cocktail (Roche) and incubated on ice for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... 0,1% sodium deoxycholate and 1% NP-40 with Complete EDTA-free protease inhibitors (Roche) and sonicated 10 s on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... The adhered cells were then gently washed with cold PBS and lysed in 1% NP-40 lysis buffer (50mM Tris-HCl pH 8.0, 150 mM sodium chloride, 1% NP-40, Roche Complete protease inhibitor). The lysate was then cleared by centrifugation at 17,000 x g for 10 min at 4°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10 mM Tris-HCl pH 7.5/140 mM NaCl/1% NP-40/1% sodium deoxycholate/0.1% sodium dodecyl sulfate (SDS)) and 150 μL of Complete Mini protease inhibitor cocktail (Roche Diagnostic Systems, Laval, Canada). Harvested cells were sonicated using three 12-second bursts of a Sonic Dismembrator (model 500 ...
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bioRxiv - Cell Biology 2022Quote: ... containing 1% NP-40 and 0.25% sodium deoxycholate plus a complete protease inhibitor cocktail (Roche) on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% NP-40 and 0.5% sodium deoxycholate) with protease inhibitor cocktail (Roche Diagnostics, Mannheim, Germany) and kept on ice for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5 % sodium deoxycholate and 1 % NP-40) supplemented with cOmplete mini EDTA-free protease inhibitors (Roche). After protein quantification using bicinchoninic acid assay (BCA ...
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bioRxiv - Immunology 2021Quote: Sorted cells or stimulated cells were lysed in RIPA buffer (50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM PMSF, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1x Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Neuroscience 2020Quote: ... The protein was extracted by adding ice-cold 10 v/w RIPA buffer (250 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris HCl, pH 8.0, containing Roche cOmplete™ protease inhibitor cocktail and PhosSTOP phosphatase inhibitor cocktail and 1mM AEBSF ...
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bioRxiv - Neuroscience 2024Quote: ... and 100,000 worms were collected in 1 ml of RIPA buffer (50 mM Tris HCl pH 7.4, 150 mM NaCl, 1% Nonidet NP-40, 0.1% SDS, 0.5% Sodium deoxycholate, Roche cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Neuroscience 2024Quote: ... The agarose resin was washed once with high salt buffer (50 mM Tris HCl pH 7.4, 1M NaCl, 1 mM EDTA, 1% Nonidet NP-40, 0.1% SDS, 0.5% Sodium deoxycholate, Roche cOmplete EDTA-free Protease Inhibitor Cocktail and RNase Inhibitor ...
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bioRxiv - Microbiology 2024Quote: ... 50CmM HEPES, 5CmM EDTA, 0.10% NP-40, 0.5CmM sodium orthovanadate, 0.5CmM NaF, protease inhibitor cocktail from Roche), and cell-free virions were purified by centrifugation of cell culture supernatants through a 20% sucrose cushion at 20,800 × g for 90 min at 4°C and lysed in co-IP lysis buffer ...
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bioRxiv - Developmental Biology 2024Quote: Pellets were thawed by adding 800 μL ice-cold lysis buffer (50 mM HEPES pH 7.5, 100 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, 1x Roche cOmplete ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was replaced with RIPA buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM MgCl2, 1% NP-40, 0.5% sodium deoxycholate, 1x complete protease inhibitor [Roche]) including 1% SDS ...
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bioRxiv - Genetics 2021Quote: ... Harvested cells in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA and 1X protease inhibitor cocktail; Roche). Anti-FLAG M2 Magnetic Beads (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: Adherent cells were washed twice with PBS and then scraped and digested on ice for 30 min with a homemade RIPA (50 mM Tris-HCl pH 7.5, 15 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate and 0.1% SDS, supplemented with Protease Inhibitors [Roche] and Phosphatase Inhibitors [Sigma-Aldrich]) ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed with RIPA buffer (25 mM Tris HCl [pH 7.6], 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche]). The lysates were subjected to SDS-PAGE under denaturing conditions in 12% polyacrylamide gels using Bio-Rad Mini PROTEAN electrophoresis system (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was aspirated and the pellet was resuspended in RIPA buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS supplemented with Roche protease and phosphatase inhibitor tablet just before use) ...
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bioRxiv - Genomics 2020Quote: ... Cells were collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, 0.1% SDS, 2 mM EDTA, 1 mM PMSF, Roche protease and phosphate inhibitor cocktails ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor (#11873580001, Roche) in PBS)39 ...
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bioRxiv - Microbiology 2020Quote: Whole cell lysates were generated by lysing cells in RIPA buffer (50 mM Tris-Cl [pH 7.4], 150 mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, 1 mM phenylmethylsulfonyl fluoride [PMSF], 1× Roche complete mini-protease inhibitor cocktail ...
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bioRxiv - Genomics 2021Quote: ... 150 mM NaCl, 2 mM EDTA pH8, 1% NP-40, 0.5% Sodium Deoxycholate, 0.1% SDS, supplemented with ROCHE Complete protease inhibitor tablets (no EDTA) ...
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bioRxiv - Developmental Biology 2022Quote: ... 150 mM NaCl, 2 mM EDTA pH8, 1% NP-40, 0.5% Sodium Deoxycholate, 0.1% SDS, supplemented with ROCHE Complete protease inhibitor tablets (no EDTA) ...
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bioRxiv - Cell Biology 2023Quote: ... 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 2x Complete EDTA-free protease inhibitor [Roche]), and insoluble material and lipid were cleared by centrifugation at 16,000 g for 15 min at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with glass beads in 250 µl RIPA buffer (25mM Tris/HCl pH 7.6, 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, Roche complete protease inhibitor cocktail ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were rinsed twice with PBS and resuspended in 1 mL lysis buffer (1X PBS, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS) supplemented with protease inhibitor cocktail (Roche, #11836170001). Chromatin was sheared to 200-500 base pairs using a Covaris E220 sonicator and cleared by centrifugation for 15 minutes at 19,000 × g ...
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bioRxiv - Biochemistry 2020Quote: ... 1% v/v NP-40, 0.1% w/v SDS, 1% w/v sodium deoxycholate, 1× complete mini-protease mixture; Roche), and incubated on ice for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... and then homogenized with a glass tissue homogenizer in lysis buffer (50 mM Tris pH 7.5, 150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate) containing protease inhibitors (Roche, 11697498001). Supernatant fractions were collected by centrifugation and boiled with 2× Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... and LiCl (250 mM LiCl, 1% NP-40, 1% sodium deoxycholate, 1 mM EDTA, 20 mM Tris-HCl PH8.0, complete protease inhibitor [Roche]) washing buffers ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 1% NP-40 and 0.25% sodium deoxycholate (modified RIPA) plus a Complete Protease Inhibitor Cocktail (Roche Applied Science) on ice ...
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bioRxiv - Bioengineering 2023Quote: Liver tissue was lysed using RIPA buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 0.1% SDS, 1% NP-40, 0.5% sodium deoxycholate, cOmplete® Mini Protease Inhibitor Cocktail [Roche Diagnosis ...
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bioRxiv - Biochemistry 2020Quote: ... 1% NP-40, 1 mM EDTA, 1 mM EGTA, 1 mM NaF, 1 mM sodium orthovanadate, 1 mM PMSF and Roche’s protease inhibitor tablet (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: Cells were lysed in lysis buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 1% Nonidet P-40, 1 mM sodium orthovanadate and 1× complete protease inhibitor cocktail from Roche) at 4°C for 15 minutes ...
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bioRxiv - Genomics 2023Quote: ... and collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor [#11873580001, Roche] in PBS). Chromatin was sonicated with a Covaris E220 sonicator (140PIP ...
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bioRxiv - Neuroscience 2023Quote: ... 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche], and PhosStop [Roche]; pH 7.5) and sonicated for 30 s ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with glass beads in 250 µl RIPA buffer (25mM Tris/HCl pH 7.6, 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, Roche complete protease inhibitor cocktail; Roche PhosStop tablet) using a FastPrep (MP biomedicals) ...
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bioRxiv - Immunology 2021Quote: ... 1mM PMSF, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1x Roche Complete Protease Inhibitor Cocktail, and 1x Roche Phosphatase Inhibitor Cocktail). Protein concentrations were measured using a BCA kit (Pierce) ...
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bioRxiv - Microbiology 2021Quote: ... The adherent cells were lysed with ASM lysis buffer (250 mM sodium acetate, pH 5.0, 0.1% Nonidet P-40, 1.3 mM EDTA, and 1x Roche Complete protease inhibitor cocktail) for at least 15 min and were scraped off with a pipette tip and collected ...
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bioRxiv - Molecular Biology 2024Quote: ... then resuspended in 880 uL RIPA buffer (1x PBS, 1% NP-40, 0.5% Sodium deoxycholate, 0.1% SGS, Roche protease inhibitor cocktail), and sheared using Covaris E220 Focused-Ultrasonicator ...
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bioRxiv - Neuroscience 2020Quote: Cells were washed with ice-cold PBS and lysed in RIPA buffer (50 mM pH 8.0 Tris-HCl, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1mM NaVO4, 1mM NaF, Roche complete protease inhibitor tablet). Lysates were cleared by centrifuging at 16,000 Xg at 4°C for 10 m ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... 1% NP-40, 0.5% sodium deoxycholate, 1 mM EDTA pH 8 and 1 tablet of protease inhibitor cocktail Roche cOmplete (Roche, Basel, Switzerland) and 1 tablet of phosphatase inhibitor PhosSTOP (Roche)) ...
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bioRxiv - Microbiology 2023Quote: ... 1% NP-40, 1 mM EDTA, 1 mM EGTA, 0.1% SDS, 1X EDTA-free protease inhibitor cocktail [#04693132001, Roche], and 0.5% sodium deoxycholate) and collected using a cell scraper ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM EDTA, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche], and PhosStop [Roche]; pH 7.5) and sonicated for 30 s ...
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bioRxiv - Molecular Biology 2023Quote: Cell pellets were lysed (75mM NaCl, 50mM HEPES pH 8.5, 10mM Sodium pyrophosphate, 10mM Sodium Fluoride, 10mM B-Glycerophosphate, 10mM Sodium Orthovanadate, Roche complete mini EDTA free protease inhibitors ...
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bioRxiv - Molecular Biology 2023Quote: ... BSA fraction V (2% wt/vol) (Roche Diagnostics), 2-mercaptoethanol (50 µM) ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.5 wt% bovine serum albumin (BSA, Roche) in PBS for 3 hours ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5% Nonidet P-40 (NP-40) and 1X protease inhibitor cocktail (Roche)] and centrifuged at 3,500×g for 15 min ...